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nebnext ultra ii dna library prep kit for illumina  (New England Biolabs)


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    New England Biolabs nebnext ultra ii dna library prep kit for illumina
    Nebnext Ultra Ii Dna Library Prep Kit For Illumina, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 12134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/enzymatic+chip-seq+kit/NEBNext+Ultra+II+DNA+Library+Prep+Kit+for+Illumina/custom%40e7645%4033397398
    Average 99 stars, based on 12134 article reviews
    nebnext ultra ii dna library prep kit for illumina - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: Gut virome remodeling after fecal microbiota transplantation is associated with virus-bacteria co-variation and metabolic response in obesity and type 2 diabetes.
    Article Snippet: .. Sequencing libraries were prepared from 1 μg DNA per sample using the NEBNext Ultra DNA Library Prep Kit (Illumina) and sequenced on an Illumina NovaSeq 6000 platform with pairedend 150 bp reads, generating a median of 49.8 million reads per sample. ..

    Article Title: A comprehensive HA-tagged PRC1 toolkit enables standardized chromatin profiling and proteomic analysis in Drosophila melanogaster.
    Article Snippet: Following decrosslinking, DNA was purified using 220 MicroChIP DiaPure columns (Diagenode). .. DNA libraries for sequencing were prepared using 221 the NEBNext Ultra II DNA Library Prep Kit for Illumina. .. Paired-end sequencing (sequencing 222 (150 bp; approximately 2 Gb per sample) was performed by Novogene 223 (https://en.novogene.com/).

    Article Title: Electroacupuncture Alleviates Comorbid Depression‐Like Behaviors and Dyspepsia‐Like Symptoms in Rats Accompanied by Alterations in Hippocampal Neuroinflammation and Gut Microbiota
    Article Snippet: The product was quantified using the Quant‐iT PicoGreen dsDNA Assay Kit. .. Sequencing libraries were constructed using the NEB Next Ultra II DNA Library Prep Kit (New England Biolabs, Inc., USA). .. After passing quality control, the library was sequenced at both ends on the Illumina NovaSeq 6000 platform (Illumina, Inc., USA).

    Article Title: Identical consensus genomes of bovine coronavirus in respiratory and enteric specimens from a single cow and sequence variation after cell culture passage.
    Article Snippet: 1 Laboratory of Veterinary Microbiology, Joint Department of Veterinary Medicine, Gifu University, Yanagido, Gifu 501-1193, Japan 2 Joint Graduate School of Veterinary Sciences, Gifu University, Yanagido, Gifu 501-1193, Japan 3 Education and Research Center for Food Animal Health, Gifu University, Yanagido, Gifu 501-1193, Japan 4 Center for One Medicine Innovative Translational Research, Gifu University, Yanagido, Gifu 501-1193, Japan 5 Gifu Prefectural Chuo Livestock Hygiene Service Center, Yanagido, Gifu 501-1193, Japan 6 Joint Graduate School of Veterinary Sciences, Gifu University, 1-1, Yanagido, Gifu 501-1193, Japan Abstract Bovine coronavirus (BCoV) causes respiratory and enteric diseases in cattle, but the relationship between viral genetic variation and anatomical distribution remains unclear.. We compared BCoV genomes detected in a tracheal swab and diarrheic intestinal content from the same adult cow.. The two clinical specimens had identical 31,015-nt consensus genomes.

    Article Title: Clonal circulating tumor cell mobilization following chimeric antigen receptor T-cell therapy in metastatic castration-resistant prostate cancer
    Article Snippet: Afterward, DNA was purified with a QIAquick PCR Purification Kit (Qiagen, Cat#. 28106) with elution in 50 μL of Tris-EDTA buffer. .. Libraries for sequencing were constructed using the NEBNext Ultra DNA Library Preparation Kit from Illumina (New England Biolabs, Cat#. E7370L), which amplified and sonicated 50 μg of the previously purified single-cell DNA. .. The library concentration and anticipated size distribution of 300–500 bp were quantified via Qubit (Thermo Fisher) and the Agilent 2100 Bioanalyzer (High-Sensitivity DNA Assay and Kit, Agilent Technologies, Cat#. 5067–4626), respectively.

    Construct:

    Article Title: Electroacupuncture Alleviates Comorbid Depression‐Like Behaviors and Dyspepsia‐Like Symptoms in Rats Accompanied by Alterations in Hippocampal Neuroinflammation and Gut Microbiota
    Article Snippet: The product was quantified using the Quant‐iT PicoGreen dsDNA Assay Kit. .. Sequencing libraries were constructed using the NEB Next Ultra II DNA Library Prep Kit (New England Biolabs, Inc., USA). .. After passing quality control, the library was sequenced at both ends on the Illumina NovaSeq 6000 platform (Illumina, Inc., USA).

    Article Title: Clonal circulating tumor cell mobilization following chimeric antigen receptor T-cell therapy in metastatic castration-resistant prostate cancer
    Article Snippet: Afterward, DNA was purified with a QIAquick PCR Purification Kit (Qiagen, Cat#. 28106) with elution in 50 μL of Tris-EDTA buffer. .. Libraries for sequencing were constructed using the NEBNext Ultra DNA Library Preparation Kit from Illumina (New England Biolabs, Cat#. E7370L), which amplified and sonicated 50 μg of the previously purified single-cell DNA. .. The library concentration and anticipated size distribution of 300–500 bp were quantified via Qubit (Thermo Fisher) and the Agilent 2100 Bioanalyzer (High-Sensitivity DNA Assay and Kit, Agilent Technologies, Cat#. 5067–4626), respectively.

    DNA Library Preparation:

    Article Title: Clonal circulating tumor cell mobilization following chimeric antigen receptor T-cell therapy in metastatic castration-resistant prostate cancer
    Article Snippet: Afterward, DNA was purified with a QIAquick PCR Purification Kit (Qiagen, Cat#. 28106) with elution in 50 μL of Tris-EDTA buffer. .. Libraries for sequencing were constructed using the NEBNext Ultra DNA Library Preparation Kit from Illumina (New England Biolabs, Cat#. E7370L), which amplified and sonicated 50 μg of the previously purified single-cell DNA. .. The library concentration and anticipated size distribution of 300–500 bp were quantified via Qubit (Thermo Fisher) and the Agilent 2100 Bioanalyzer (High-Sensitivity DNA Assay and Kit, Agilent Technologies, Cat#. 5067–4626), respectively.

    Amplification:

    Article Title: Clonal circulating tumor cell mobilization following chimeric antigen receptor T-cell therapy in metastatic castration-resistant prostate cancer
    Article Snippet: Afterward, DNA was purified with a QIAquick PCR Purification Kit (Qiagen, Cat#. 28106) with elution in 50 μL of Tris-EDTA buffer. .. Libraries for sequencing were constructed using the NEBNext Ultra DNA Library Preparation Kit from Illumina (New England Biolabs, Cat#. E7370L), which amplified and sonicated 50 μg of the previously purified single-cell DNA. .. The library concentration and anticipated size distribution of 300–500 bp were quantified via Qubit (Thermo Fisher) and the Agilent 2100 Bioanalyzer (High-Sensitivity DNA Assay and Kit, Agilent Technologies, Cat#. 5067–4626), respectively.

    Sonication:

    Article Title: Clonal circulating tumor cell mobilization following chimeric antigen receptor T-cell therapy in metastatic castration-resistant prostate cancer
    Article Snippet: Afterward, DNA was purified with a QIAquick PCR Purification Kit (Qiagen, Cat#. 28106) with elution in 50 μL of Tris-EDTA buffer. .. Libraries for sequencing were constructed using the NEBNext Ultra DNA Library Preparation Kit from Illumina (New England Biolabs, Cat#. E7370L), which amplified and sonicated 50 μg of the previously purified single-cell DNA. .. The library concentration and anticipated size distribution of 300–500 bp were quantified via Qubit (Thermo Fisher) and the Agilent 2100 Bioanalyzer (High-Sensitivity DNA Assay and Kit, Agilent Technologies, Cat#. 5067–4626), respectively.

    Purification:

    Article Title: Clonal circulating tumor cell mobilization following chimeric antigen receptor T-cell therapy in metastatic castration-resistant prostate cancer
    Article Snippet: Afterward, DNA was purified with a QIAquick PCR Purification Kit (Qiagen, Cat#. 28106) with elution in 50 μL of Tris-EDTA buffer. .. Libraries for sequencing were constructed using the NEBNext Ultra DNA Library Preparation Kit from Illumina (New England Biolabs, Cat#. E7370L), which amplified and sonicated 50 μg of the previously purified single-cell DNA. .. The library concentration and anticipated size distribution of 300–500 bp were quantified via Qubit (Thermo Fisher) and the Agilent 2100 Bioanalyzer (High-Sensitivity DNA Assay and Kit, Agilent Technologies, Cat#. 5067–4626), respectively.

    Single Cell:

    Article Title: Clonal circulating tumor cell mobilization following chimeric antigen receptor T-cell therapy in metastatic castration-resistant prostate cancer
    Article Snippet: Afterward, DNA was purified with a QIAquick PCR Purification Kit (Qiagen, Cat#. 28106) with elution in 50 μL of Tris-EDTA buffer. .. Libraries for sequencing were constructed using the NEBNext Ultra DNA Library Preparation Kit from Illumina (New England Biolabs, Cat#. E7370L), which amplified and sonicated 50 μg of the previously purified single-cell DNA. .. The library concentration and anticipated size distribution of 300–500 bp were quantified via Qubit (Thermo Fisher) and the Agilent 2100 Bioanalyzer (High-Sensitivity DNA Assay and Kit, Agilent Technologies, Cat#. 5067–4626), respectively.



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    Lactylation-driven expression of an oncogene set. a A heatmap depicts alterations in lactylation-associated gene expression and chromatin accessibility pre- and post-lactate treatment in the oral squamous cell carcinoma (OSCC) cell line CAL27. b Correlation analysis between lactylation-driven gene expression levels and chromatin accessibility. c Variations in lactylation-driven genes and chromatin accessibility in CAL27 cells exposed to high lactate concentrations. d Enrichment analysis of lactylation-driven genes and gene sets positively and negatively correlated with chromatin accessibility via single-sample gene set enrichment analysis (ssGSEA). e Intersection analysis of lactylation-driven gene sets positively correlated with chromatin accessibility and differentially expressed genes, determined by cleavage under targets and tagmentation <t>sequencing</t> (CUT&TAG-seq), assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq), and RNA sequencing (RNA-seq). f Validation of increased lactylation enrichment in the promoter regions of CASP3 and TPR in OSCC tissues through chromatin immunoprecipitation-quantitative polymerase chain reaction <t>(ChIP-qPCR,</t> two-tailed unpaired Student’s t -test, ** P < 0.01) and prediction of promoter sequences potentially affected by lactylation. g Validation of elevated expression of CASP3 , HSP90AA1 , HSP90B1 , TPR , RAS , and CCND1 in OSCC tissues through reverse transcription-quantitative polymerase chain reaction (RT-qPCR, two-tailed paired Student’s t -test, * P < 0.05; ** P < 0.01). Lac: lactate
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    Image Search Results


    Lactylation-driven expression of an oncogene set. a A heatmap depicts alterations in lactylation-associated gene expression and chromatin accessibility pre- and post-lactate treatment in the oral squamous cell carcinoma (OSCC) cell line CAL27. b Correlation analysis between lactylation-driven gene expression levels and chromatin accessibility. c Variations in lactylation-driven genes and chromatin accessibility in CAL27 cells exposed to high lactate concentrations. d Enrichment analysis of lactylation-driven genes and gene sets positively and negatively correlated with chromatin accessibility via single-sample gene set enrichment analysis (ssGSEA). e Intersection analysis of lactylation-driven gene sets positively correlated with chromatin accessibility and differentially expressed genes, determined by cleavage under targets and tagmentation sequencing (CUT&TAG-seq), assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq), and RNA sequencing (RNA-seq). f Validation of increased lactylation enrichment in the promoter regions of CASP3 and TPR in OSCC tissues through chromatin immunoprecipitation-quantitative polymerase chain reaction (ChIP-qPCR, two-tailed unpaired Student’s t -test, ** P < 0.01) and prediction of promoter sequences potentially affected by lactylation. g Validation of elevated expression of CASP3 , HSP90AA1 , HSP90B1 , TPR , RAS , and CCND1 in OSCC tissues through reverse transcription-quantitative polymerase chain reaction (RT-qPCR, two-tailed paired Student’s t -test, * P < 0.05; ** P < 0.01). Lac: lactate

    Journal: Genome Biology

    Article Title: Multi-omics reveals lactylation-driven regulatory mechanisms promoting tumor progression in oral squamous cell carcinoma

    doi: 10.1186/s13059-024-03383-8

    Figure Lengend Snippet: Lactylation-driven expression of an oncogene set. a A heatmap depicts alterations in lactylation-associated gene expression and chromatin accessibility pre- and post-lactate treatment in the oral squamous cell carcinoma (OSCC) cell line CAL27. b Correlation analysis between lactylation-driven gene expression levels and chromatin accessibility. c Variations in lactylation-driven genes and chromatin accessibility in CAL27 cells exposed to high lactate concentrations. d Enrichment analysis of lactylation-driven genes and gene sets positively and negatively correlated with chromatin accessibility via single-sample gene set enrichment analysis (ssGSEA). e Intersection analysis of lactylation-driven gene sets positively correlated with chromatin accessibility and differentially expressed genes, determined by cleavage under targets and tagmentation sequencing (CUT&TAG-seq), assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq), and RNA sequencing (RNA-seq). f Validation of increased lactylation enrichment in the promoter regions of CASP3 and TPR in OSCC tissues through chromatin immunoprecipitation-quantitative polymerase chain reaction (ChIP-qPCR, two-tailed unpaired Student’s t -test, ** P < 0.01) and prediction of promoter sequences potentially affected by lactylation. g Validation of elevated expression of CASP3 , HSP90AA1 , HSP90B1 , TPR , RAS , and CCND1 in OSCC tissues through reverse transcription-quantitative polymerase chain reaction (RT-qPCR, two-tailed paired Student’s t -test, * P < 0.05; ** P < 0.01). Lac: lactate

    Article Snippet: ChIP sequencing (ChIP-seq) was conducted using a ChIP-seq kit (9005S, Cell Signaling Technology) according to the provided protocol.

    Techniques: Expressing, Gene Expression, Sequencing, Next-Generation Sequencing, RNA Sequencing, Biomarker Discovery, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, ChIP-qPCR, Two Tailed Test, Reverse Transcription, Quantitative RT-PCR

    Journal: Cell Reports

    Article Title: Integrator-Dependent and Allosteric/Intrinsic Mechanisms Ensure Efficient Termination of snRNA Transcription

    doi: 10.1016/j.celrep.2020.108319

    Figure Lengend Snippet:

    Article Snippet: ChIP-seq Sample preparation kit , Cell Signaling Technologies , 9003S.

    Techniques: Recombinant, Sample Prep, Sequencing, Plasmid Preparation, Gene Expression, Control, RNA Sequencing, Western Blot, Software