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rvfv  (ProSci Incorporated)


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    Structured Review

    ProSci Incorporated rvfv
    Fig. 1. Increased ROS in <t>RVFV</t> infected HepG2 cells. (A) HepG2 cells were infected with the MP-12 strain of RVFV (MOI: 3) and stained after 3 h using MitoSox stain and DAPI. The stained cells were visualized by confocal microscopy. (B) HepG2 cells infected with MP-12 (MOI: 3) were lysed at 24 h post infection and total protein lysate obtained. Lysates from uninfected cells were obtained as controls and both lysates were resolved by SDS-PAGE and western blots, carried out using anti-RVFV and <t>anti-β-actin</t> <t>antibodies.</t>
    Rvfv, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/envelope+prosci/Rift+Valley+Fever+Virus+Antibody/pm24418562-362-3-4
    Average 90 stars, based on 7 article reviews
    rvfv - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Reactive oxygen species activate NFκB (p65) and p53 and induce apoptosis in RVFV infected liver cells."

    Article Title: Reactive oxygen species activate NFκB (p65) and p53 and induce apoptosis in RVFV infected liver cells.

    Journal: Virology

    doi: 10.1016/j.virol.2013.11.023

    Fig. 1. Increased ROS in RVFV infected HepG2 cells. (A) HepG2 cells were infected with the MP-12 strain of RVFV (MOI: 3) and stained after 3 h using MitoSox stain and DAPI. The stained cells were visualized by confocal microscopy. (B) HepG2 cells infected with MP-12 (MOI: 3) were lysed at 24 h post infection and total protein lysate obtained. Lysates from uninfected cells were obtained as controls and both lysates were resolved by SDS-PAGE and western blots, carried out using anti-RVFV and anti-β-actin antibodies.
    Figure Legend Snippet: Fig. 1. Increased ROS in RVFV infected HepG2 cells. (A) HepG2 cells were infected with the MP-12 strain of RVFV (MOI: 3) and stained after 3 h using MitoSox stain and DAPI. The stained cells were visualized by confocal microscopy. (B) HepG2 cells infected with MP-12 (MOI: 3) were lysed at 24 h post infection and total protein lysate obtained. Lysates from uninfected cells were obtained as controls and both lysates were resolved by SDS-PAGE and western blots, carried out using anti-RVFV and anti-β-actin antibodies.

    Techniques Used: Infection, Staining, Confocal Microscopy, SDS Page, Western Blot

    Related Articles

    Incubation:

    Article Title: Curcumin Inhibits Rift Valley Fever Virus Replication in Human Cells
    Article Snippet: .. Primary antibodies to RVFV (ProSci, catalog No. 4519), Total p65 (Abcam, catalog No. ab7970), phospho-p65 (Ser-536; Santa Cruz Biotechnology, Inc., catalog No. 33020), phospho-IκBα (Santa Cruz Biotechnology, catalog No. sc-21869), HRP-conjugated actin (Abcam, catalog No. ab49900), IKK-α (Santa Cruz Biotechnology, catalog No. sc-7182), IKK-β (Santa Cruz Biotechnology, catalog No. sc-7329), and IKK-γ (Cell Signaling, catalog No. 2685P) were used according to manufacturer's instructions, and the blots were incubated overnight at 4 °C. .. The blots were then washed three times with PBS-T and incubated with secondary HRP-coupled goat anti-rabbit antibody (Cell Signaling, catalog No. 7074).

    Article Title: Protein Phosphatase-1 Regulates Rift Valley Fever Virus Replication
    Article Snippet: .. Membranes were probed with anti-RVFV N, anti-RVFV Gn (#4519, ProSci), anti-V5 (Serotec), anti-Flag (Sigma Aldritch), anti-PP1α and β (Cell Signaling), anti-PP1β (Abcam), anti-peIF2α (S51) (Cell Signaling) and HRP conjugated actin, diluted in blocking buffer, then incubated overnight at 4°C. ..

    Article Title: An equine herpesvirus type 1 (EHV-1) vector expressing Rift Valley fever virus (RVFV) Gn and Gc induces neutralizing antibodies in sheep
    Article Snippet: .. RK13 cells were infected either with rH_Gn-Gc or rH for 24 h and then incubated with rabbit anti-RVFV(CT) (ProSci catalog no. 4521) or rabbit anti-RVFV(IN) (ProSci catalog no. 4519), that recognize the RVFV Gn or Gc, respectively, for 1 h at RT. .. After extensive washing with PBS, the secondary antibody, anti-rabbit IgG conjugated with Alexa 488 (Invitrogen), was added at a 1:500 dilution and incubated for 30 min at RT.

    Article Title: Reactive oxygen species activate NFκB (p65) and p53 and induce apoptosis in RVFV infected liver cells.
    Article Snippet: .. Primary antibodies to RVFV (ProSci, Cat# 4519), total p65 (Santa Cruz Biotechnology, Inc. Cat# sc7151), phospho-p65 (ser536) (Santacruz biotechnology, Inc., Cat# 33020), total p53 (Cell Signaling, Cat# 9282S), phospho-p53 (Ser15) (Cell Signaling, Cat# 9284S), Cytochrome C (Abcam, Cat# ab13575) and HRP conjugated actin (Abcam, Cat# ab49900) were used according to manufacturer’s instructions and the blots were incubated overnight at 4 1C. .. The blots were washed three times with PBS-T and incubated with secondary HRP coupled goat anti-rabbit antibody (Cell Signaling, Cat# 7074).

    Cell-Signaling:

    Article Title: Protein Phosphatase-1 Regulates Rift Valley Fever Virus Replication
    Article Snippet: .. Membranes were probed with anti-RVFV N, anti-RVFV Gn (#4519, ProSci), anti-V5 (Serotec), anti-Flag (Sigma Aldritch), anti-PP1α and β (Cell Signaling), anti-PP1β (Abcam), anti-peIF2α (S51) (Cell Signaling) and HRP conjugated actin, diluted in blocking buffer, then incubated overnight at 4°C. ..

    Blocking Assay:

    Article Title: Protein Phosphatase-1 Regulates Rift Valley Fever Virus Replication
    Article Snippet: .. Membranes were probed with anti-RVFV N, anti-RVFV Gn (#4519, ProSci), anti-V5 (Serotec), anti-Flag (Sigma Aldritch), anti-PP1α and β (Cell Signaling), anti-PP1β (Abcam), anti-peIF2α (S51) (Cell Signaling) and HRP conjugated actin, diluted in blocking buffer, then incubated overnight at 4°C. ..

    other:

    Article Title: Curcumin Inhibits Rift Valley Fever Virus Replication in Human Cells
    Article Snippet: To prepare whole cell extracts, the supernatant was removed from the wells, and cells were lysed in lysis buffer (1:1 mixture of T-PER reagent (Pierce), 2× Tris-glycine SDS sample buffer (Novex, Invitrogen), 2.5% β-mercaptoethanol, and a protease and phosphatase inhibitor mixture (1× Halt mixture, Pierce)) and boiled for 10 min prior to electrophoresis.

    Infection:

    Article Title: An equine herpesvirus type 1 (EHV-1) vector expressing Rift Valley fever virus (RVFV) Gn and Gc induces neutralizing antibodies in sheep
    Article Snippet: .. RK13 cells were infected either with rH_Gn-Gc or rH for 24 h and then incubated with rabbit anti-RVFV(CT) (ProSci catalog no. 4521) or rabbit anti-RVFV(IN) (ProSci catalog no. 4519), that recognize the RVFV Gn or Gc, respectively, for 1 h at RT. .. After extensive washing with PBS, the secondary antibody, anti-rabbit IgG conjugated with Alexa 488 (Invitrogen), was added at a 1:500 dilution and incubated for 30 min at RT.



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