Review



emyhc  (Developmental Studies Hybridoma Bank)


Bioz Verified Symbol Developmental Studies Hybridoma Bank is a verified supplier
Bioz Manufacturer Symbol Developmental Studies Hybridoma Bank manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Developmental Studies Hybridoma Bank emyhc
    Emyhc, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/emyhc/anti-Myosin+heavy+chain/pm42014394-237-28-29
    Average 96 stars, based on 19 article reviews
    emyhc - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Immunofluorescence:

    Article Title: EMILIN1 emerges as a TGFβ/SETDB1-regulated secreted biomarker in Duchenne muscular dystrophy.
    Article Snippet: Widefield fluorescence images were taken with an Axio Observer 7 AR TI CL E IN P RE SS microscope (Zeiss) equipped with a motorized stage coupled to an Orca Flash 4 Camera (Hamamatsu) and driven by the Zen software (Zeiss). .. Antibodies Antibody Reference Use and dilution Myosin heavy chain, MYH1E (mouse IgG2b) DSHB MF20 Immunofluorescence (1:10) PAI1 (rabbit) Proteintech, 13801-1-AP Western blot (1:1000) MyoD (rabbit) Cell Signaling Technology, 13812 Western blot (1:1000) Vinculin (mouse IgG1) Sigma-Aldrich, V9131 Western blot (1:500) Emilin1 (rabbit) Kind gift from Paola Spessotto, As-556 Immunofluorescence (1:1000) Emilin1 (rabbit) Abcam, AB185953 Immunofluorescence (1:100) Laminin (rabbit) Dako, Z0097 Immunofluorescence (1:400) .. Total RNA from cells was extracted using RNeasy Micro Kit (Qiagen, 74004) following the manufacturer’s procedures.

    Western Blot:

    Article Title: EMILIN1 emerges as a TGFβ/SETDB1-regulated secreted biomarker in Duchenne muscular dystrophy.
    Article Snippet: Widefield fluorescence images were taken with an Axio Observer 7 AR TI CL E IN P RE SS microscope (Zeiss) equipped with a motorized stage coupled to an Orca Flash 4 Camera (Hamamatsu) and driven by the Zen software (Zeiss). .. Antibodies Antibody Reference Use and dilution Myosin heavy chain, MYH1E (mouse IgG2b) DSHB MF20 Immunofluorescence (1:10) PAI1 (rabbit) Proteintech, 13801-1-AP Western blot (1:1000) MyoD (rabbit) Cell Signaling Technology, 13812 Western blot (1:1000) Vinculin (mouse IgG1) Sigma-Aldrich, V9131 Western blot (1:500) Emilin1 (rabbit) Kind gift from Paola Spessotto, As-556 Immunofluorescence (1:1000) Emilin1 (rabbit) Abcam, AB185953 Immunofluorescence (1:100) Laminin (rabbit) Dako, Z0097 Immunofluorescence (1:400) .. Total RNA from cells was extracted using RNeasy Micro Kit (Qiagen, 74004) following the manufacturer’s procedures.

    Article Title: PGC-1α pathway dysregulation disrupts myofiber specification in a mouse model of SBMA.
    Article Snippet: .. The following primary antibodies (antigen, dilution/concentration, vendor, catalog number) were used for these studies: Myh4, 1:100, Developmental Studies Hybridoma Bank at the University of Iowa (DSHB), BF-F3; Myh1, 1:10, DSHB, 6H1; PGC-1α (ChIP-seq), Novus, NBP1-04676; PGC-1α (Western blot), 1:1000, Abcam, ab191838; Vinculin, 1:10,000, Sigma Aldrich, V9131; MEF2A/C, 1:100, Abcam, ab197070; CBP, 1:200, Invitrogen, PA5-27369; CREB, 1:500, Cell Signaling Technology, D76D11; p62, 1:400, PROGEN, GP62-C; PG-21 (AR), 1:500, Millipore, 06- 680. ..

    Article Title: Diaphragm hypertrophy and spinal stress response accompany phrenic myelin defects in a murine model of Charcot-Marie-Tooth Disease Type 1E.
    Article Snippet: Charcot-Marie-Tooth disease type 1E (CMT1E) is a rare, early-onset dysmyelinating neuropathy linked with point mutations in the peripheral myelin protein 22 (PMP22) gene.. Respiratory problems are known to impact neuropathic patients, yet existing studies have not characterized this pathophysiology in corresponding animal models.. Trembler J (TrJ) mice carry the same Leu16Pro amino acid substitution in the PMP22 protein that is present in families diagnosed with CMT1E.

    Pyrolysis Gas Chromatography:

    Article Title: PGC-1α pathway dysregulation disrupts myofiber specification in a mouse model of SBMA.
    Article Snippet: .. The following primary antibodies (antigen, dilution/concentration, vendor, catalog number) were used for these studies: Myh4, 1:100, Developmental Studies Hybridoma Bank at the University of Iowa (DSHB), BF-F3; Myh1, 1:10, DSHB, 6H1; PGC-1α (ChIP-seq), Novus, NBP1-04676; PGC-1α (Western blot), 1:1000, Abcam, ab191838; Vinculin, 1:10,000, Sigma Aldrich, V9131; MEF2A/C, 1:100, Abcam, ab197070; CBP, 1:200, Invitrogen, PA5-27369; CREB, 1:500, Cell Signaling Technology, D76D11; p62, 1:400, PROGEN, GP62-C; PG-21 (AR), 1:500, Millipore, 06- 680. ..

    Chromatin Immunoprecipitation:

    Article Title: PGC-1α pathway dysregulation disrupts myofiber specification in a mouse model of SBMA.
    Article Snippet: .. The following primary antibodies (antigen, dilution/concentration, vendor, catalog number) were used for these studies: Myh4, 1:100, Developmental Studies Hybridoma Bank at the University of Iowa (DSHB), BF-F3; Myh1, 1:10, DSHB, 6H1; PGC-1α (ChIP-seq), Novus, NBP1-04676; PGC-1α (Western blot), 1:1000, Abcam, ab191838; Vinculin, 1:10,000, Sigma Aldrich, V9131; MEF2A/C, 1:100, Abcam, ab197070; CBP, 1:200, Invitrogen, PA5-27369; CREB, 1:500, Cell Signaling Technology, D76D11; p62, 1:400, PROGEN, GP62-C; PG-21 (AR), 1:500, Millipore, 06- 680. ..

    other:

    Article Title:
    Article Snippet: Mouse monoclonal anti-eMyHC , DSHB , Cat# F1.652, RRID: AB_528358.

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals
    Article Snippet: The fiber type of individual single fiber segments was determined using the dot blotting method previously described and now termed MyDoBID (Myosin heavy chain detection by Dot Blotting for IDentification of muscle fiber type)., Briefly, a polyvinylidene fluoride (PVDF) membrane was activated in 96% ethanol for 120 s and equilibrated in transfer buffer (Bio-Rad Laboratories Laboratories, Hercules, CA, USA) for 120 s. Following this, 1-μL (10%) of each fiber was spotted on activated PVDF membrane.

    Immunostaining:

    Article Title: Diaphragm hypertrophy and spinal stress response accompany phrenic myelin defects in a murine model of Charcot-Marie-Tooth Disease Type 1E.
    Article Snippet: Charcot-Marie-Tooth disease type 1E (CMT1E) is a rare, early-onset dysmyelinating neuropathy linked with point mutations in the peripheral myelin protein 22 (PMP22) gene.. Respiratory problems are known to impact neuropathic patients, yet existing studies have not characterized this pathophysiology in corresponding animal models.. Trembler J (TrJ) mice carry the same Leu16Pro amino acid substitution in the PMP22 protein that is present in families diagnosed with CMT1E.

    Blocking Assay:

    Article Title: Gut microbiota-derived taurolithocholic acid modulates myofiber-type switching via p38 MAPK/PGC-1α signaling underlying breed differences between Arbor Acres and Taoyuan chickens
    Article Snippet: .. Following blocking with 1% BSA, the sections were incubated with the primary antibody MYH1A (1:100, F59, DSHB, USA) and the AF647-labeled goat anti-mouse secondary antibody (1:500, A0473, Beyotime), with the primary antibody MYH7B (1:100, S58, DSHB) and the FITC-labeled goat anti-mouse secondary antibody (1:500, A0568, Beyotime). .. Cell nuclei were stained with DAPI (C1002, Beyotime).

    Incubation:

    Article Title: Gut microbiota-derived taurolithocholic acid modulates myofiber-type switching via p38 MAPK/PGC-1α signaling underlying breed differences between Arbor Acres and Taoyuan chickens
    Article Snippet: .. Following blocking with 1% BSA, the sections were incubated with the primary antibody MYH1A (1:100, F59, DSHB, USA) and the AF647-labeled goat anti-mouse secondary antibody (1:500, A0473, Beyotime), with the primary antibody MYH7B (1:100, S58, DSHB) and the FITC-labeled goat anti-mouse secondary antibody (1:500, A0568, Beyotime). .. Cell nuclei were stained with DAPI (C1002, Beyotime).



    Similar Products

    96
    Developmental Studies Hybridoma Bank emyhc
    Emyhc, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/emyhc/anti-Myosin+heavy+chain/pm42014394-237-28-29
    Average 96 stars, based on 1 article reviews
    emyhc - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    96
    Developmental Studies Hybridoma Bank mouse anti emyhc
    Mouse Anti Emyhc, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/emyhc/anti-Myosin+heavy+chain/pm41957353-480-15-17
    Average 96 stars, based on 1 article reviews
    mouse anti emyhc - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    96
    Developmental Studies Hybridoma Bank nd emyhc
    Nd Emyhc, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/emyhc/anti-Myosin+heavy+chain/pm41874466-233-47-49
    Average 96 stars, based on 1 article reviews
    nd emyhc - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    93
    Proteintech anti mouse embryonic myosin heavy chain emyhc
    ( A ) Experimental timeline of the transfer experiment. DT (400 ng/mouse) was administered intraperitoneally on the indicated days to LysM-Cre iDTR mice to induce macrophage depletion. Ly6C hi CX3CR1 lo PDPN + CD9 + MDMs and other MDMs (the remaining MDM population) were isolated from muscle tissues of WT mice at 1 dpi. The isolated cells, suspended in 10 μL PBS or the same volume of PBS alone, were then injected intramuscularly into the recipient mice. ( B and C ) Representative images of H&E ( B ) and immunofluorescence staining ( C ) of muscle sections from DT-treated LysM-Cre iDTR mice injected intramuscularly with PBS ( n = 3), other MDMs ( n = 5), or Ly6C hi CX3CR1 lo PDPN + CD9 + MDMs ( n = 6) at 4 dpi. Muscle sections were stained <t>with</t> <t>anti-mouse</t> <t>eMyHC</t> (red) to mark regenerating muscle fibers and Hoechst (blue) to label nuclei. The boxed areas in the main images are shown at higher magnification (×4) in the adjacent lower panels. H&E staining and corresponding immunofluorescence images represent the same field of view. Scale bars: 100 μm. ( D and E ) Quantification of muscle regeneration showing the distribution of the CSA of eMyHC + fiber ( D ) and the mean CSA of eMyHC + fibers in an individual mouse ( E ). The P values were calculated using 1-way ANOVA with Tukey’s multiple-comparison test. A P value < 0.05 was considered significant. Data are shown as the mean ± SEM, and symbols represent individual fibers ( D ) or individual mice ( E ).
    Anti Mouse Embryonic Myosin Heavy Chain Emyhc, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/emyhc/MYH3+Antibody/pmc12948425-237-10-18
    Average 93 stars, based on 1 article reviews
    anti mouse embryonic myosin heavy chain emyhc - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    96
    Developmental Studies Hybridoma Bank antibodies against emyhc
    ( A ) Experimental timeline of the transfer experiment. DT (400 ng/mouse) was administered intraperitoneally on the indicated days to LysM-Cre iDTR mice to induce macrophage depletion. Ly6C hi CX3CR1 lo PDPN + CD9 + MDMs and other MDMs (the remaining MDM population) were isolated from muscle tissues of WT mice at 1 dpi. The isolated cells, suspended in 10 μL PBS or the same volume of PBS alone, were then injected intramuscularly into the recipient mice. ( B and C ) Representative images of H&E ( B ) and immunofluorescence staining ( C ) of muscle sections from DT-treated LysM-Cre iDTR mice injected intramuscularly with PBS ( n = 3), other MDMs ( n = 5), or Ly6C hi CX3CR1 lo PDPN + CD9 + MDMs ( n = 6) at 4 dpi. Muscle sections were stained <t>with</t> <t>anti-mouse</t> <t>eMyHC</t> (red) to mark regenerating muscle fibers and Hoechst (blue) to label nuclei. The boxed areas in the main images are shown at higher magnification (×4) in the adjacent lower panels. H&E staining and corresponding immunofluorescence images represent the same field of view. Scale bars: 100 μm. ( D and E ) Quantification of muscle regeneration showing the distribution of the CSA of eMyHC + fiber ( D ) and the mean CSA of eMyHC + fibers in an individual mouse ( E ). The P values were calculated using 1-way ANOVA with Tukey’s multiple-comparison test. A P value < 0.05 was considered significant. Data are shown as the mean ± SEM, and symbols represent individual fibers ( D ) or individual mice ( E ).
    Antibodies Against Emyhc, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/emyhc/anti-Myosin+heavy+chain/pmc12877386-100-56-60
    Average 96 stars, based on 1 article reviews
    antibodies against emyhc - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    Image Search Results


    ( A ) Experimental timeline of the transfer experiment. DT (400 ng/mouse) was administered intraperitoneally on the indicated days to LysM-Cre iDTR mice to induce macrophage depletion. Ly6C hi CX3CR1 lo PDPN + CD9 + MDMs and other MDMs (the remaining MDM population) were isolated from muscle tissues of WT mice at 1 dpi. The isolated cells, suspended in 10 μL PBS or the same volume of PBS alone, were then injected intramuscularly into the recipient mice. ( B and C ) Representative images of H&E ( B ) and immunofluorescence staining ( C ) of muscle sections from DT-treated LysM-Cre iDTR mice injected intramuscularly with PBS ( n = 3), other MDMs ( n = 5), or Ly6C hi CX3CR1 lo PDPN + CD9 + MDMs ( n = 6) at 4 dpi. Muscle sections were stained with anti-mouse eMyHC (red) to mark regenerating muscle fibers and Hoechst (blue) to label nuclei. The boxed areas in the main images are shown at higher magnification (×4) in the adjacent lower panels. H&E staining and corresponding immunofluorescence images represent the same field of view. Scale bars: 100 μm. ( D and E ) Quantification of muscle regeneration showing the distribution of the CSA of eMyHC + fiber ( D ) and the mean CSA of eMyHC + fibers in an individual mouse ( E ). The P values were calculated using 1-way ANOVA with Tukey’s multiple-comparison test. A P value < 0.05 was considered significant. Data are shown as the mean ± SEM, and symbols represent individual fibers ( D ) or individual mice ( E ).

    Journal: The Journal of Clinical Investigation

    Article Title: Activin A secretion by muscle-repairing macrophages induces heterotopic ossification in mice

    doi: 10.1172/JCI193797

    Figure Lengend Snippet: ( A ) Experimental timeline of the transfer experiment. DT (400 ng/mouse) was administered intraperitoneally on the indicated days to LysM-Cre iDTR mice to induce macrophage depletion. Ly6C hi CX3CR1 lo PDPN + CD9 + MDMs and other MDMs (the remaining MDM population) were isolated from muscle tissues of WT mice at 1 dpi. The isolated cells, suspended in 10 μL PBS or the same volume of PBS alone, were then injected intramuscularly into the recipient mice. ( B and C ) Representative images of H&E ( B ) and immunofluorescence staining ( C ) of muscle sections from DT-treated LysM-Cre iDTR mice injected intramuscularly with PBS ( n = 3), other MDMs ( n = 5), or Ly6C hi CX3CR1 lo PDPN + CD9 + MDMs ( n = 6) at 4 dpi. Muscle sections were stained with anti-mouse eMyHC (red) to mark regenerating muscle fibers and Hoechst (blue) to label nuclei. The boxed areas in the main images are shown at higher magnification (×4) in the adjacent lower panels. H&E staining and corresponding immunofluorescence images represent the same field of view. Scale bars: 100 μm. ( D and E ) Quantification of muscle regeneration showing the distribution of the CSA of eMyHC + fiber ( D ) and the mean CSA of eMyHC + fibers in an individual mouse ( E ). The P values were calculated using 1-way ANOVA with Tukey’s multiple-comparison test. A P value < 0.05 was considered significant. Data are shown as the mean ± SEM, and symbols represent individual fibers ( D ) or individual mice ( E ).

    Article Snippet: For the evaluation of muscle regeneration, sections were stained with anti-mouse embryonic myosin heavy chain (eMyHC) (catalog 22287-1-AP; Proteintech) and corresponding Alexa Fluor 594 secondary antibody (catalog A-11012; Thermo Fisher Scientific) to visualize regenerating fibers.

    Techniques: Isolation, Injection, Immunofluorescence, Staining, Comparison

    ( A and B ) Representative images of H&E and immunofluorescence staining of muscle sections from Inhba fl/fl mice ( n = 8) and Inhba fl/fl LysM-Cre mice ( n = 8) at 4 dpi. In immunofluorescence staining, sections were stained for anti-mouse eMyHC (red) to indicate regenerating fibers and Hoechst (blue) to indicate nuclei. The boxed areas are magnified (×4) in the panels to the right. H&E staining and corresponding immunofluorescence images represent the same field of view. Scale bars: 100 μm. ( C – E ) Quantification of regeneration showing the distribution of the CSA of eMyHC + fiber ( C ), the mean CSA of eMyHC + fibers in an individual mouse ( D ), and the percentage of regeneration area (calculated as total eMyHC + area/injured area) ( E ). ( F and G ) Representative images of H&E and immunofluorescence staining of muscle sections from Inhba fl/fl mice ( n = 8) and Inhba fl/fl LysM-Cre mice ( n = 8) at 4 dpi. In immunofluorescence staining, sections were stained for mIgG (white) to indicate necrotic fibers. H&E staining and corresponding immunofluorescence images represent the same field of view. Scale bars: 50 μm. ( H ) The density of mIgG + fibers (calculated as the number mIgG + fibers/injured area). ( I ) Grip strength of the forelimb in Inhba fl/fl and LysM-Cre Inhba fl/fl mice at 7 dpi. gf, gram force. ( J and K ) Photographs of gastrocnemius of the Inhba fl/fl ( J ) and LysM-Cre Inhba fl/fl ( K ) mice at 14 dpi. Scale bars: 5 mm. ( L ) Mass of gastrocnemius of Inhba fl/fl and LysM-Cre Inhba fl/fl mice at 14 dpi. The P values were calculated using unpaired 2-tailed t test. A P value < 0.05 was considered significant. Data are shown as the mean ± SEM, and symbols represent individual fibers ( C ) or individual mice ( D , E , H , I , and L ).

    Journal: The Journal of Clinical Investigation

    Article Title: Activin A secretion by muscle-repairing macrophages induces heterotopic ossification in mice

    doi: 10.1172/JCI193797

    Figure Lengend Snippet: ( A and B ) Representative images of H&E and immunofluorescence staining of muscle sections from Inhba fl/fl mice ( n = 8) and Inhba fl/fl LysM-Cre mice ( n = 8) at 4 dpi. In immunofluorescence staining, sections were stained for anti-mouse eMyHC (red) to indicate regenerating fibers and Hoechst (blue) to indicate nuclei. The boxed areas are magnified (×4) in the panels to the right. H&E staining and corresponding immunofluorescence images represent the same field of view. Scale bars: 100 μm. ( C – E ) Quantification of regeneration showing the distribution of the CSA of eMyHC + fiber ( C ), the mean CSA of eMyHC + fibers in an individual mouse ( D ), and the percentage of regeneration area (calculated as total eMyHC + area/injured area) ( E ). ( F and G ) Representative images of H&E and immunofluorescence staining of muscle sections from Inhba fl/fl mice ( n = 8) and Inhba fl/fl LysM-Cre mice ( n = 8) at 4 dpi. In immunofluorescence staining, sections were stained for mIgG (white) to indicate necrotic fibers. H&E staining and corresponding immunofluorescence images represent the same field of view. Scale bars: 50 μm. ( H ) The density of mIgG + fibers (calculated as the number mIgG + fibers/injured area). ( I ) Grip strength of the forelimb in Inhba fl/fl and LysM-Cre Inhba fl/fl mice at 7 dpi. gf, gram force. ( J and K ) Photographs of gastrocnemius of the Inhba fl/fl ( J ) and LysM-Cre Inhba fl/fl ( K ) mice at 14 dpi. Scale bars: 5 mm. ( L ) Mass of gastrocnemius of Inhba fl/fl and LysM-Cre Inhba fl/fl mice at 14 dpi. The P values were calculated using unpaired 2-tailed t test. A P value < 0.05 was considered significant. Data are shown as the mean ± SEM, and symbols represent individual fibers ( C ) or individual mice ( D , E , H , I , and L ).

    Article Snippet: For the evaluation of muscle regeneration, sections were stained with anti-mouse embryonic myosin heavy chain (eMyHC) (catalog 22287-1-AP; Proteintech) and corresponding Alexa Fluor 594 secondary antibody (catalog A-11012; Thermo Fisher Scientific) to visualize regenerating fibers.

    Techniques: Immunofluorescence, Staining

    ( A – C ) H&E and immunofluorescence staining of muscle sections from WT ( A ; n = 4), Tlr4 –/– ( B ; n = 5), and Ticam1 –/– mice ( C ; n = 5) at 4 dpi. Immunofluorescence staining showing eMyHC-labeled (red) regenerating muscle fibers and Hoechst-stained (blue) nuclei. The boxed areas are shown at higher magnification (×4) in the panels to the right. H&E staining and the corresponding immunofluorescence images represent the same field. Scale bars: 100 μm. ( D and E ) Quantification of regeneration showing the distribution of eMyHC + CSA ( D ) and the mean CSA of eMyHC + fibers ( E ). The P values were calculated using 1-way ANOVA with Tukey’s multiple-comparison test. A P value < 0.05 was considered significant. Data are shown as the mean ± SEM, and symbols represent individual fibers ( D ) or individual mice ( E ).

    Journal: The Journal of Clinical Investigation

    Article Title: Activin A secretion by muscle-repairing macrophages induces heterotopic ossification in mice

    doi: 10.1172/JCI193797

    Figure Lengend Snippet: ( A – C ) H&E and immunofluorescence staining of muscle sections from WT ( A ; n = 4), Tlr4 –/– ( B ; n = 5), and Ticam1 –/– mice ( C ; n = 5) at 4 dpi. Immunofluorescence staining showing eMyHC-labeled (red) regenerating muscle fibers and Hoechst-stained (blue) nuclei. The boxed areas are shown at higher magnification (×4) in the panels to the right. H&E staining and the corresponding immunofluorescence images represent the same field. Scale bars: 100 μm. ( D and E ) Quantification of regeneration showing the distribution of eMyHC + CSA ( D ) and the mean CSA of eMyHC + fibers ( E ). The P values were calculated using 1-way ANOVA with Tukey’s multiple-comparison test. A P value < 0.05 was considered significant. Data are shown as the mean ± SEM, and symbols represent individual fibers ( D ) or individual mice ( E ).

    Article Snippet: For the evaluation of muscle regeneration, sections were stained with anti-mouse embryonic myosin heavy chain (eMyHC) (catalog 22287-1-AP; Proteintech) and corresponding Alexa Fluor 594 secondary antibody (catalog A-11012; Thermo Fisher Scientific) to visualize regenerating fibers.

    Techniques: Immunofluorescence, Staining, Labeling, Comparison