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ezh2 gene expression  (Addgene inc)


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    Structured Review

    Addgene inc ezh2 gene expression
    Ezh2 Gene Expression, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector/pFUGW-H1+empty+vector+(Plasmid+%2325870)/us11938129-1194-14-20
    Average 93 stars, based on 27 article reviews
    ezh2 gene expression - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Isolation:

    Article Title: Methods and compositions for the treatment of vascular disease
    Article Snippet: .. At passage one, isolated VSMC cells were transduced with lentivirus overexpressing CMV-cre to deplete Ezh2 gene expression (pFUGW-H1 empty vector, Addgene plasmid #25870). ..

    Transduction:

    Article Title: Methods and compositions for the treatment of vascular disease
    Article Snippet: .. At passage one, isolated VSMC cells were transduced with lentivirus overexpressing CMV-cre to deplete Ezh2 gene expression (pFUGW-H1 empty vector, Addgene plasmid #25870). ..

    Gene Expression:

    Article Title: Methods and compositions for the treatment of vascular disease
    Article Snippet: .. At passage one, isolated VSMC cells were transduced with lentivirus overexpressing CMV-cre to deplete Ezh2 gene expression (pFUGW-H1 empty vector, Addgene plasmid #25870). ..

    Plasmid Preparation:

    Article Title: Methods and compositions for the treatment of vascular disease
    Article Snippet: .. At passage one, isolated VSMC cells were transduced with lentivirus overexpressing CMV-cre to deplete Ezh2 gene expression (pFUGW-H1 empty vector, Addgene plasmid #25870). ..

    Article Title: Whole-brain in vivo base editing reverses autistic-like behaviors in mice
    Article Snippet: In addition, two different shRNA were inserted into FUGW-H1 vector (Addgene plasmid 25870) for knock-down assays. .. In addition, two different shRNA were inserted into FUGW-H1 vector (Addgene plasmid 25870) for knock-down assays. ..

    Article Title: Whole-brain in vivo base editing reverses behavioral changes in Mef2c-mutant mice.
    Article Snippet: .. In addition, two different shRNA were inserted into the FUGW-H1 vector (Addgene plasmid no. 25870) for the knockdown assays. ..

    Article Title: Whole-brain in vivo base editing reverses autistic-like behaviors in mice
    Article Snippet: In addition, two different shRNA were inserted into FUGW-H1 vector (Addgene plasmid 25870) for knock-down assays. .. In addition, two different shRNA were inserted into FUGW-H1 vector (Addgene plasmid 25870) for knock-down assays. ..

    Article Title: Novel IL1RAP mutation associated with schizophrenia interferes with neuronal growth and related NF-κB signal pathways.
    Article Snippet: Schizophrenia is a complex, severe psychiatric disorder with a high heritability that affects approximately 1% of the world’s population.. Numerous schizophrenia-related risk genes have been reported in large-scale studies, but the role of most genetic abnormalities in the pathogenesis of the disease is still obscure.. In this study, using whole-exome sequencing, we identified a novel nonsense mutation c.1324C > T in the Interleukin 1 receptor accessory protein (IL1RAP) gene in four affected individuals with schizophrenia of a Chinese family.

    Article Title: Tet1 Suppresses p21 to Ensure Proper Cell Cycle Progression in Embryonic Stem Cells
    Article Snippet: .. HEK293T cells were cultured in 10% FBS Dulbecco’s Modified Eagle Medium (DMEM) and transfected with a 10 μg pFUGW-H1 empty vector or pFUGW-H1 p21 shRNA1 (Addgene Watertown, MA, USA, plasmid # 25868) [ ] along with lentiviral packaging plasmids pPAX2 (7.5 μg) and pMDG (2.5 μg) using an Xtremegene Transfection reagent (06365787001, Roche, Basel, Switzerland). .. The media were changed after 16 h. The following day, the lentivirus-containing supernatant was collected at 24 h and at 48 h and concentrated by a Lenti-X Concentrator (631232,Takara, Tokyo, Japan), according to the manufacturer’s protocol.

    shRNA:

    Article Title: Whole-brain in vivo base editing reverses autistic-like behaviors in mice
    Article Snippet: In addition, two different shRNA were inserted into FUGW-H1 vector (Addgene plasmid 25870) for knock-down assays. .. In addition, two different shRNA were inserted into FUGW-H1 vector (Addgene plasmid 25870) for knock-down assays. ..

    Article Title: Whole-brain in vivo base editing reverses behavioral changes in Mef2c-mutant mice.
    Article Snippet: .. In addition, two different shRNA were inserted into the FUGW-H1 vector (Addgene plasmid no. 25870) for the knockdown assays. ..

    Article Title: Whole-brain in vivo base editing reverses autistic-like behaviors in mice
    Article Snippet: In addition, two different shRNA were inserted into FUGW-H1 vector (Addgene plasmid 25870) for knock-down assays. .. In addition, two different shRNA were inserted into FUGW-H1 vector (Addgene plasmid 25870) for knock-down assays. ..

    Knockdown:

    Article Title: Whole-brain in vivo base editing reverses autistic-like behaviors in mice
    Article Snippet: In addition, two different shRNA were inserted into FUGW-H1 vector (Addgene plasmid 25870) for knock-down assays. .. In addition, two different shRNA were inserted into FUGW-H1 vector (Addgene plasmid 25870) for knock-down assays. ..

    Article Title: Whole-brain in vivo base editing reverses behavioral changes in Mef2c-mutant mice.
    Article Snippet: .. In addition, two different shRNA were inserted into the FUGW-H1 vector (Addgene plasmid no. 25870) for the knockdown assays. ..

    Article Title: Whole-brain in vivo base editing reverses autistic-like behaviors in mice
    Article Snippet: In addition, two different shRNA were inserted into FUGW-H1 vector (Addgene plasmid 25870) for knock-down assays. .. In addition, two different shRNA were inserted into FUGW-H1 vector (Addgene plasmid 25870) for knock-down assays. ..

    Clone Assay:

    Article Title: Novel IL1RAP mutation associated with schizophrenia interferes with neuronal growth and related NF-κB signal pathways.
    Article Snippet: Schizophrenia is a complex, severe psychiatric disorder with a high heritability that affects approximately 1% of the world’s population.. Numerous schizophrenia-related risk genes have been reported in large-scale studies, but the role of most genetic abnormalities in the pathogenesis of the disease is still obscure.. In this study, using whole-exome sequencing, we identified a novel nonsense mutation c.1324C > T in the Interleukin 1 receptor accessory protein (IL1RAP) gene in four affected individuals with schizophrenia of a Chinese family.

    Control:

    Article Title: Novel IL1RAP mutation associated with schizophrenia interferes with neuronal growth and related NF-κB signal pathways.
    Article Snippet: Schizophrenia is a complex, severe psychiatric disorder with a high heritability that affects approximately 1% of the world’s population.. Numerous schizophrenia-related risk genes have been reported in large-scale studies, but the role of most genetic abnormalities in the pathogenesis of the disease is still obscure.. In this study, using whole-exome sequencing, we identified a novel nonsense mutation c.1324C > T in the Interleukin 1 receptor accessory protein (IL1RAP) gene in four affected individuals with schizophrenia of a Chinese family.

    Cell Culture:

    Article Title: Tet1 Suppresses p21 to Ensure Proper Cell Cycle Progression in Embryonic Stem Cells
    Article Snippet: .. HEK293T cells were cultured in 10% FBS Dulbecco’s Modified Eagle Medium (DMEM) and transfected with a 10 μg pFUGW-H1 empty vector or pFUGW-H1 p21 shRNA1 (Addgene Watertown, MA, USA, plasmid # 25868) [ ] along with lentiviral packaging plasmids pPAX2 (7.5 μg) and pMDG (2.5 μg) using an Xtremegene Transfection reagent (06365787001, Roche, Basel, Switzerland). .. The media were changed after 16 h. The following day, the lentivirus-containing supernatant was collected at 24 h and at 48 h and concentrated by a Lenti-X Concentrator (631232,Takara, Tokyo, Japan), according to the manufacturer’s protocol.

    Modification:

    Article Title: Tet1 Suppresses p21 to Ensure Proper Cell Cycle Progression in Embryonic Stem Cells
    Article Snippet: .. HEK293T cells were cultured in 10% FBS Dulbecco’s Modified Eagle Medium (DMEM) and transfected with a 10 μg pFUGW-H1 empty vector or pFUGW-H1 p21 shRNA1 (Addgene Watertown, MA, USA, plasmid # 25868) [ ] along with lentiviral packaging plasmids pPAX2 (7.5 μg) and pMDG (2.5 μg) using an Xtremegene Transfection reagent (06365787001, Roche, Basel, Switzerland). .. The media were changed after 16 h. The following day, the lentivirus-containing supernatant was collected at 24 h and at 48 h and concentrated by a Lenti-X Concentrator (631232,Takara, Tokyo, Japan), according to the manufacturer’s protocol.

    Transfection:

    Article Title: Tet1 Suppresses p21 to Ensure Proper Cell Cycle Progression in Embryonic Stem Cells
    Article Snippet: .. HEK293T cells were cultured in 10% FBS Dulbecco’s Modified Eagle Medium (DMEM) and transfected with a 10 μg pFUGW-H1 empty vector or pFUGW-H1 p21 shRNA1 (Addgene Watertown, MA, USA, plasmid # 25868) [ ] along with lentiviral packaging plasmids pPAX2 (7.5 μg) and pMDG (2.5 μg) using an Xtremegene Transfection reagent (06365787001, Roche, Basel, Switzerland). .. The media were changed after 16 h. The following day, the lentivirus-containing supernatant was collected at 24 h and at 48 h and concentrated by a Lenti-X Concentrator (631232,Takara, Tokyo, Japan), according to the manufacturer’s protocol.



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    Image Search Results


    a, HCT116 cells stably expressing BRIP1 R162Q were transiently transfected using PEI with empty vector (EV-EGFP) or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.

    Journal: bioRxiv

    Article Title: Role of a childhood cancer-linked BRIP1/FANCJ germline variant in genomic instability and cancer cell vulnerability

    doi: 10.64898/2026.03.24.714005

    Figure Lengend Snippet: a, HCT116 cells stably expressing BRIP1 R162Q were transiently transfected using PEI with empty vector (EV-EGFP) or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.

    Article Snippet: For R-loop removal, cells were transiently transfected with an EGFP empty vector control (pEGFP-C1, Takara Bio, #632470), EGFP-tagged RNaseH1 wild-type (WT), or the catalytically inactive RNaseH1 D145N mutant as indicated.

    Techniques: Stable Transfection, Expressing, Transfection, Plasmid Preparation, Fluorescence, Western Blot, Construct, Immunofluorescence, Labeling, Confocal Microscopy, Two Tailed Test