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3xflag tag  (Addgene inc)


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    Structured Review

    Addgene inc 3xflag tag
    3xflag Tag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector/CMV500+empty+vector+(Plasmid+%2333348)/us11220678-829-22-298
    Average 90 stars, based on 2 article reviews
    3xflag tag - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Role of Nudt2 in Anchorage-Independent Growth and Cell Migration of Human Melanoma
    Article Snippet: .. A mixture of vsvg/viral envelope plasmid, dvpr/viral packaging vector, PLX304 empty vector (Addgene) or Nudt2 WT vector or PLX304-Luciferase-V5 vector were co-transfected into the 293T cells. ..

    Article Title: Lin28a induces SOX9 and chondrocyte reprogramming via HMGA2 and blunts cartilage loss in mice
    Article Snippet: .. RNA-seq was performed in duplicate for the following conditions: primary murine chondrocytes were transduced for control conditions with empty vector (CMV500 empty vector; Addgene plasmid no. 33348) and for Lin28 overexpression with pMSCV-mLin28A (Addgene plasmid no. 26357). .. Total RNA was extracted and transferred for sequencing to IntegraGen SA Co. (Evry, France).

    Article Title: Fulminant Viral Hepatitis in Two Siblings with Inherited IL-10RB Deficiency.
    Article Snippet: Cells were transfected in the presence of Lipofectamine LTX (Invitrogen), in accordance with the manufacturer’s instructions. .. The pLX302-IL10RA-V5 plasmid and pLX302 empty vector were purchased from Addgene. pCMV3-IL22RA1C-His, pCMV3-SP-Myc-IL-10RB, and pCMV3 empty vector were purchased from Sinobiological. .. All other vectors were purchased from Origene: pCMV6-IL10RB-Myc-DDK, pCMV6-IFNLR1-Myc-DDK (transcript variant 1), pCMV6IL20RA-Myc-DDK, and pCMV6 empty vector.

    Article Title: CCDC50 mediates the clearance of protein aggregates to prevent cellular proteotoxicity.
    Article Snippet: CCDC50 mediates the clearance of protein aggregates to prevent cellular proteotoxicity Yu Ye, Penghui Jia, Jiafan Miao, Yicheng Wang, Zibo Li, Yuxin Lin, Miao He, Shurui Liu, Bi-Rong Zheng, Junyu Wu, Ji’an Pan, Chun-Mei Li, Panpan Hou & Deyin Guo To cite this article: Yu Ye, Penghui Jia, Jiafan Miao, Yicheng Wang, Zibo Li, Yuxin Lin, Miao He, Shurui Liu, Bi-Rong Zheng, Junyu Wu, Ji’an Pan, Chun-Mei Li, Panpan Hou & Deyin Guo (13 Jun 2024): CCDC50 mediates the clearance of protein aggregates to prevent cellular proteotoxicity, Autophagy, DOI: 10.1080/15548627.2024.2367183 To link to this article: https://doi.org/10.1080/15548627.2024.2367183

    Article Title: Lin28a induces SOX9 and chondrocyte reprogramming via HMGA2 and blunts cartilage loss in mice.
    Article Snippet: .. RNA-seq was performed in duplicate for the following conditions: primary murine chondrocytes were transduced for control conditions with empty vector (CMV500 empty vector; Addgene plasmid no. 33348) and for Lin28 overexpression with pMSCV-mLin28A (Addgene plasmid no. 26357). .. Total RNA was extracted and transferred for sequencing to IntegraGen SA Co. (Evry, France).

    Article Title: Lin28a induces SOX9 and chondrocyte reprogramming via HMGA2 and blunts cartilage loss in mice.
    Article Snippet: Ready-to-use packaging plasmid mix (Cellectar, Florham Park, NJ, USA) and ExGen 500 (Euromedex, Souffelweyersheim, France) transfection reagents were used for plasmid transfection. .. Plasmids (2 g) for Lin-28a (pMSCV-mLin28A, Addgene plasmid no. 26357) and HMGA2 (pBabe zeo HMGA2, Addgene plasmid no. 17411; pLKO.shHmga2, Addgene plasmid no. 32399) or empty vector (CMV500 empty vector, Addgene plasmid no. 33348) were transfected into cells. ..

    RNA Sequencing:

    Article Title: Lin28a induces SOX9 and chondrocyte reprogramming via HMGA2 and blunts cartilage loss in mice
    Article Snippet: .. RNA-seq was performed in duplicate for the following conditions: primary murine chondrocytes were transduced for control conditions with empty vector (CMV500 empty vector; Addgene plasmid no. 33348) and for Lin28 overexpression with pMSCV-mLin28A (Addgene plasmid no. 26357). .. Total RNA was extracted and transferred for sequencing to IntegraGen SA Co. (Evry, France).

    Article Title: Lin28a induces SOX9 and chondrocyte reprogramming via HMGA2 and blunts cartilage loss in mice.
    Article Snippet: .. RNA-seq was performed in duplicate for the following conditions: primary murine chondrocytes were transduced for control conditions with empty vector (CMV500 empty vector; Addgene plasmid no. 33348) and for Lin28 overexpression with pMSCV-mLin28A (Addgene plasmid no. 26357). .. Total RNA was extracted and transferred for sequencing to IntegraGen SA Co. (Evry, France).

    Control:

    Article Title: Lin28a induces SOX9 and chondrocyte reprogramming via HMGA2 and blunts cartilage loss in mice
    Article Snippet: .. RNA-seq was performed in duplicate for the following conditions: primary murine chondrocytes were transduced for control conditions with empty vector (CMV500 empty vector; Addgene plasmid no. 33348) and for Lin28 overexpression with pMSCV-mLin28A (Addgene plasmid no. 26357). .. Total RNA was extracted and transferred for sequencing to IntegraGen SA Co. (Evry, France).

    Article Title: CCDC50 mediates the clearance of protein aggregates to prevent cellular proteotoxicity.
    Article Snippet: CCDC50 mediates the clearance of protein aggregates to prevent cellular proteotoxicity Yu Ye, Penghui Jia, Jiafan Miao, Yicheng Wang, Zibo Li, Yuxin Lin, Miao He, Shurui Liu, Bi-Rong Zheng, Junyu Wu, Ji’an Pan, Chun-Mei Li, Panpan Hou & Deyin Guo To cite this article: Yu Ye, Penghui Jia, Jiafan Miao, Yicheng Wang, Zibo Li, Yuxin Lin, Miao He, Shurui Liu, Bi-Rong Zheng, Junyu Wu, Ji’an Pan, Chun-Mei Li, Panpan Hou & Deyin Guo (13 Jun 2024): CCDC50 mediates the clearance of protein aggregates to prevent cellular proteotoxicity, Autophagy, DOI: 10.1080/15548627.2024.2367183 To link to this article: https://doi.org/10.1080/15548627.2024.2367183

    Article Title: Lin28a induces SOX9 and chondrocyte reprogramming via HMGA2 and blunts cartilage loss in mice.
    Article Snippet: .. RNA-seq was performed in duplicate for the following conditions: primary murine chondrocytes were transduced for control conditions with empty vector (CMV500 empty vector; Addgene plasmid no. 33348) and for Lin28 overexpression with pMSCV-mLin28A (Addgene plasmid no. 26357). .. Total RNA was extracted and transferred for sequencing to IntegraGen SA Co. (Evry, France).

    Over Expression:

    Article Title: Lin28a induces SOX9 and chondrocyte reprogramming via HMGA2 and blunts cartilage loss in mice
    Article Snippet: .. RNA-seq was performed in duplicate for the following conditions: primary murine chondrocytes were transduced for control conditions with empty vector (CMV500 empty vector; Addgene plasmid no. 33348) and for Lin28 overexpression with pMSCV-mLin28A (Addgene plasmid no. 26357). .. Total RNA was extracted and transferred for sequencing to IntegraGen SA Co. (Evry, France).

    Article Title: Lin28a induces SOX9 and chondrocyte reprogramming via HMGA2 and blunts cartilage loss in mice.
    Article Snippet: .. RNA-seq was performed in duplicate for the following conditions: primary murine chondrocytes were transduced for control conditions with empty vector (CMV500 empty vector; Addgene plasmid no. 33348) and for Lin28 overexpression with pMSCV-mLin28A (Addgene plasmid no. 26357). .. Total RNA was extracted and transferred for sequencing to IntegraGen SA Co. (Evry, France).

    Generated:

    Article Title: CCDC50 mediates the clearance of protein aggregates to prevent cellular proteotoxicity.
    Article Snippet: CCDC50 mediates the clearance of protein aggregates to prevent cellular proteotoxicity Yu Ye, Penghui Jia, Jiafan Miao, Yicheng Wang, Zibo Li, Yuxin Lin, Miao He, Shurui Liu, Bi-Rong Zheng, Junyu Wu, Ji’an Pan, Chun-Mei Li, Panpan Hou & Deyin Guo To cite this article: Yu Ye, Penghui Jia, Jiafan Miao, Yicheng Wang, Zibo Li, Yuxin Lin, Miao He, Shurui Liu, Bi-Rong Zheng, Junyu Wu, Ji’an Pan, Chun-Mei Li, Panpan Hou & Deyin Guo (13 Jun 2024): CCDC50 mediates the clearance of protein aggregates to prevent cellular proteotoxicity, Autophagy, DOI: 10.1080/15548627.2024.2367183 To link to this article: https://doi.org/10.1080/15548627.2024.2367183

    Transfection:

    Article Title: Microvesicles-delivering Smad7 have advantages over microvesicles in suppressing fibroblast differentiation in a model of Peyronie’s disease
    Article Snippet: .. According to previous methods [ ], the serum starvation-induced BMSCs were transfected with 1 μg PEI25k/pCMV5-Smad7 vector or empty PEI25k/pCMV5 vector (Addgene, Cambridge, MA, USA) for 24 h. The transfected BMSCs were cultured for 48 h. ..

    Article Title: Lin28a induces SOX9 and chondrocyte reprogramming via HMGA2 and blunts cartilage loss in mice.
    Article Snippet: Ready-to-use packaging plasmid mix (Cellectar, Florham Park, NJ, USA) and ExGen 500 (Euromedex, Souffelweyersheim, France) transfection reagents were used for plasmid transfection. .. Plasmids (2 g) for Lin-28a (pMSCV-mLin28A, Addgene plasmid no. 26357) and HMGA2 (pBabe zeo HMGA2, Addgene plasmid no. 17411; pLKO.shHmga2, Addgene plasmid no. 32399) or empty vector (CMV500 empty vector, Addgene plasmid no. 33348) were transfected into cells. ..

    Cell Culture:

    Article Title: Microvesicles-delivering Smad7 have advantages over microvesicles in suppressing fibroblast differentiation in a model of Peyronie’s disease
    Article Snippet: .. According to previous methods [ ], the serum starvation-induced BMSCs were transfected with 1 μg PEI25k/pCMV5-Smad7 vector or empty PEI25k/pCMV5 vector (Addgene, Cambridge, MA, USA) for 24 h. The transfected BMSCs were cultured for 48 h. ..



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    Image Search Results


    a, HCT116 cells stably expressing BRIP1 R162Q were transiently transfected using PEI with empty vector (EV-EGFP) or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.

    Journal: bioRxiv

    Article Title: Role of a childhood cancer-linked BRIP1/FANCJ germline variant in genomic instability and cancer cell vulnerability

    doi: 10.64898/2026.03.24.714005

    Figure Lengend Snippet: a, HCT116 cells stably expressing BRIP1 R162Q were transiently transfected using PEI with empty vector (EV-EGFP) or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.

    Article Snippet: For R-loop removal, cells were transiently transfected with an EGFP empty vector control (pEGFP-C1, Takara Bio, #632470), EGFP-tagged RNaseH1 wild-type (WT), or the catalytically inactive RNaseH1 D145N mutant as indicated.

    Techniques: Stable Transfection, Expressing, Transfection, Plasmid Preparation, Fluorescence, Western Blot, Construct, Immunofluorescence, Labeling, Confocal Microscopy, Two Tailed Test