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empty vector plasmid  (OriGene)


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    Structured Review

    OriGene empty vector plasmid
    Empty Vector Plasmid, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 1675 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector/pCMV6-Entry+Mammalian+Expression+Vector/pmc13191034-111-4-13
    Average 96 stars, based on 1675 article reviews
    empty vector plasmid - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Overexpression of FOXO1 may suppress proliferation and migration and correlate with tumor immune cells in nasopharyngeal carcinoma
    Article Snippet: NP69 cells were cultured in Keratinocyte-SFM medium supplemented with recombinant epidermal growth factor and bovine pituitary extract (Gibco, 17005042). .. PCMV6-entry-FOXO1 plasmid (RC200477) and empty vector plasmid ( PS100001 ) were purchased from OriGene Technologies in Wuxi. .. The Cell Counting Kit-8 (CCK-8) assay (Dojindo, CK04) and a colony formation assay were used to evaluate the impact of FOXO1 on cell proliferation and colony formation, respectively.

    Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling
    Article Snippet: Mouse Nrp1 Plasmid , Origene , Cat# MR211197. .. pCMV6-Entry Plasmid , Origene , Cat# PS100001. .. Mouse Aldh1a3 CRISPR Plasmid , Santa Cruz Biotechnology , Cat# sc-425322.

    Article Title: Glutaredoxin-1 attenuates transactive response DNA-binding protein 43–induced neurotoxicity by suppressing oxidative stress and transactive response DNA-binding protein 43 aggregation
    Article Snippet: Plasmids and siRNAs were transfected to cells using Lipofectamine 2000 (Invitrogen, Waltham, Massachusetts, USA) and Lipofectamine RNAiMAX (Invitrogen), respectively. .. The coding sequences of full-length human wild-type TDP-43 (hTDP-43 , NM_007375 ) and Grx1( NM_002064.3 ) were cloned into pAAV-MCS (VPK-410, Cell Biolabs, San Diego, California, USA) and pCMV6-AC-Myc-DDK vector ( PS100001 , Origene Technologies, Rockville, Maryland, USA), respectively. .. Grx1-specific siRNA (D-001810-10-05) and negative control siRNAs (L-063838-01-0005) were purchased from Dharmacon.

    Article Title: Glutaredoxin-1 attenuates transactive response DNA-binding protein 43–induced neurotoxicity by suppressing oxidative stress and transactive response DNA-binding protein 43 aggregation
    Article Snippet: Plasmids and siRNAs were transfected to cells using Lipofectamine 2000 (Invitrogen, Waltham, Massachusetts, USA) and Lipofectamine RNAiMAX (Invitrogen), respectively. .. The coding sequences of fulllength human wild-type TDP-43 (hTDP-43, NM_007375) and Grx1(NM_002064.3) were cloned into pAAV-MCS (VPK-410, Cell Biolabs, San Diego, California, USA) and pCMV6-AC-Myc-DDK vector (PS100001, Origene Technologies, Rockville, Maryland, USA), respectively. .. Grx1-specific siRNA (D-001810-10-05) and negative control siRNAs (L-063838-01-0005) were purchased from Dharmacon.

    Article Title: Dual-site phosphorylation of SLX4 stabilizes the SLX4–MUS81 interface to promote mitotic SMX assembly and genome protection
    Article Snippet: .. To construct plasmid pCMV6 Venus-3XFLAG-SLX4, Venus-3XFLAG-SLX4resist M1A was amplified with primers HDW0910 and HDW0911 and cloned into the SalI and PmeI sites of pCMV6-Entry (Origene) using seamless cloning. ..

    Cell Culture:

    Article Title: Combination of antibody-pyrrolobenzodiazepine derivative conjugate and PARP inhibitor
    Article Snippet: .. Human CLDN3/pCMV6-Entry, human CLDN4/pCMV6-Entry, human CLDN6/pCMV-Entry, human CLDN9/pCMV6-Entry, or pCMV6-Entry purchased from OriGene Technologies, Inc. was transiently transferred into 293T cells (Thermo Fisher Scientific, HCL4517) by using Lipofectamine 2000 (Thermo Fisher Scientific), and the cells were cultured under conditions of 37° C. and 5% CO2 overnight, and then a cell suspension was prepared. .. The transfected 293T cell suspension was centrifuged to remove the supernatant, and a mouse anti-CLDN6 antibody (clone number: B1 or C7) or a mouse IgG1 control antibody (R&D Systems, Inc.) was then added and suspended to a final concentration of 30 μg/mL, 10 μg/mL, 3.3 μg/mL, or 1.1 μg/mL, and the resultant was left to stand at 4° C. for 1 hour.

    Suspension:

    Article Title: Combination of antibody-pyrrolobenzodiazepine derivative conjugate and PARP inhibitor
    Article Snippet: .. Human CLDN3/pCMV6-Entry, human CLDN4/pCMV6-Entry, human CLDN6/pCMV-Entry, human CLDN9/pCMV6-Entry, or pCMV6-Entry purchased from OriGene Technologies, Inc. was transiently transferred into 293T cells (Thermo Fisher Scientific, HCL4517) by using Lipofectamine 2000 (Thermo Fisher Scientific), and the cells were cultured under conditions of 37° C. and 5% CO2 overnight, and then a cell suspension was prepared. .. The transfected 293T cell suspension was centrifuged to remove the supernatant, and a mouse anti-CLDN6 antibody (clone number: B1 or C7) or a mouse IgG1 control antibody (R&D Systems, Inc.) was then added and suspended to a final concentration of 30 μg/mL, 10 μg/mL, 3.3 μg/mL, or 1.1 μg/mL, and the resultant was left to stand at 4° C. for 1 hour.

    Immunocytochemistry:

    Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2.
    Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy.

    Flow Cytometry:

    Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2.
    Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy.

    Western Blot:

    Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2.
    Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy.

    Expressing:

    Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2.
    Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy.

    Construct:

    Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2.
    Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy.

    Article Title: Dual-site phosphorylation of SLX4 stabilizes the SLX4–MUS81 interface to promote mitotic SMX assembly and genome protection
    Article Snippet: .. To construct plasmid pCMV6 Venus-3XFLAG-SLX4, Venus-3XFLAG-SLX4resist M1A was amplified with primers HDW0910 and HDW0911 and cloned into the SalI and PmeI sites of pCMV6-Entry (Origene) using seamless cloning. ..

    Clone Assay:

    Article Title: Glutaredoxin-1 attenuates transactive response DNA-binding protein 43–induced neurotoxicity by suppressing oxidative stress and transactive response DNA-binding protein 43 aggregation
    Article Snippet: Plasmids and siRNAs were transfected to cells using Lipofectamine 2000 (Invitrogen, Waltham, Massachusetts, USA) and Lipofectamine RNAiMAX (Invitrogen), respectively. .. The coding sequences of full-length human wild-type TDP-43 (hTDP-43 , NM_007375 ) and Grx1( NM_002064.3 ) were cloned into pAAV-MCS (VPK-410, Cell Biolabs, San Diego, California, USA) and pCMV6-AC-Myc-DDK vector ( PS100001 , Origene Technologies, Rockville, Maryland, USA), respectively. .. Grx1-specific siRNA (D-001810-10-05) and negative control siRNAs (L-063838-01-0005) were purchased from Dharmacon.

    Article Title: Glutaredoxin-1 attenuates transactive response DNA-binding protein 43–induced neurotoxicity by suppressing oxidative stress and transactive response DNA-binding protein 43 aggregation
    Article Snippet: Plasmids and siRNAs were transfected to cells using Lipofectamine 2000 (Invitrogen, Waltham, Massachusetts, USA) and Lipofectamine RNAiMAX (Invitrogen), respectively. .. The coding sequences of fulllength human wild-type TDP-43 (hTDP-43, NM_007375) and Grx1(NM_002064.3) were cloned into pAAV-MCS (VPK-410, Cell Biolabs, San Diego, California, USA) and pCMV6-AC-Myc-DDK vector (PS100001, Origene Technologies, Rockville, Maryland, USA), respectively. .. Grx1-specific siRNA (D-001810-10-05) and negative control siRNAs (L-063838-01-0005) were purchased from Dharmacon.

    Article Title: Dual-site phosphorylation of SLX4 stabilizes the SLX4–MUS81 interface to promote mitotic SMX assembly and genome protection
    Article Snippet: .. To construct plasmid pCMV6 Venus-3XFLAG-SLX4, Venus-3XFLAG-SLX4resist M1A was amplified with primers HDW0910 and HDW0911 and cloned into the SalI and PmeI sites of pCMV6-Entry (Origene) using seamless cloning. ..

    Over Expression:

    Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Other BGISEQ BGI Tech, Shenzhen, China N/A Agilent Seahorse XF24 bioanalyzer Agilent Technologies, CA, USA N/A Fluorescence microscope Olympus, Tokyo, Japan N/A iScience 29, 115950, June 19, 2026 e3 .. For stable overexpression of Aldh1a3, Plxnb1, or Nrp1, 908 or 399 cells were transfected with 2 μg of either mouse cDNA ORF clone or control ORF clone (MG222097, MR222673, MR211197, and PS100001, Origene). .. Subsequently, 0.5 mg/mL of geneticin (10131027, Thermo Fisher Scientific) was supplemented to the culture medium for selection.

    Transfection:

    Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Other BGISEQ BGI Tech, Shenzhen, China N/A Agilent Seahorse XF24 bioanalyzer Agilent Technologies, CA, USA N/A Fluorescence microscope Olympus, Tokyo, Japan N/A iScience 29, 115950, June 19, 2026 e3 .. For stable overexpression of Aldh1a3, Plxnb1, or Nrp1, 908 or 399 cells were transfected with 2 μg of either mouse cDNA ORF clone or control ORF clone (MG222097, MR222673, MR211197, and PS100001, Origene). .. Subsequently, 0.5 mg/mL of geneticin (10131027, Thermo Fisher Scientific) was supplemented to the culture medium for selection.

    Control:

    Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Other BGISEQ BGI Tech, Shenzhen, China N/A Agilent Seahorse XF24 bioanalyzer Agilent Technologies, CA, USA N/A Fluorescence microscope Olympus, Tokyo, Japan N/A iScience 29, 115950, June 19, 2026 e3 .. For stable overexpression of Aldh1a3, Plxnb1, or Nrp1, 908 or 399 cells were transfected with 2 μg of either mouse cDNA ORF clone or control ORF clone (MG222097, MR222673, MR211197, and PS100001, Origene). .. Subsequently, 0.5 mg/mL of geneticin (10131027, Thermo Fisher Scientific) was supplemented to the culture medium for selection.

    Amplification:

    Article Title: Dual-site phosphorylation of SLX4 stabilizes the SLX4–MUS81 interface to promote mitotic SMX assembly and genome protection
    Article Snippet: .. To construct plasmid pCMV6 Venus-3XFLAG-SLX4, Venus-3XFLAG-SLX4resist M1A was amplified with primers HDW0910 and HDW0911 and cloned into the SalI and PmeI sites of pCMV6-Entry (Origene) using seamless cloning. ..

    Cloning:

    Article Title: Dual-site phosphorylation of SLX4 stabilizes the SLX4–MUS81 interface to promote mitotic SMX assembly and genome protection
    Article Snippet: .. To construct plasmid pCMV6 Venus-3XFLAG-SLX4, Venus-3XFLAG-SLX4resist M1A was amplified with primers HDW0910 and HDW0911 and cloned into the SalI and PmeI sites of pCMV6-Entry (Origene) using seamless cloning. ..



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    Image Search Results


    a, HCT116 cells stably expressing BRIP1 R162Q were transiently transfected using PEI with empty vector (EV-EGFP) or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.

    Journal: bioRxiv

    Article Title: Role of a childhood cancer-linked BRIP1/FANCJ germline variant in genomic instability and cancer cell vulnerability

    doi: 10.64898/2026.03.24.714005

    Figure Lengend Snippet: a, HCT116 cells stably expressing BRIP1 R162Q were transiently transfected using PEI with empty vector (EV-EGFP) or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.

    Article Snippet: For R-loop removal, cells were transiently transfected with an EGFP empty vector control (pEGFP-C1, Takara Bio, #632470), EGFP-tagged RNaseH1 wild-type (WT), or the catalytically inactive RNaseH1 D145N mutant as indicated.

    Techniques: Stable Transfection, Expressing, Transfection, Plasmid Preparation, Fluorescence, Western Blot, Construct, Immunofluorescence, Labeling, Confocal Microscopy, Two Tailed Test