empty vector plasmid (OriGene)
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Empty Vector Plasmid, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 1675 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+vector/pCMV6-Entry+Mammalian+Expression+Vector/pmc13191034-111-4-13
Average 96 stars, based on 1675 article reviews
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Plasmid Preparation:Article Title: Overexpression of FOXO1 may suppress proliferation and migration and correlate with tumor immune cells in nasopharyngeal carcinoma Article Snippet: NP69 cells were cultured in Keratinocyte-SFM medium supplemented with recombinant epidermal growth factor and bovine pituitary extract (Gibco, 17005042). .. PCMV6-entry-FOXO1 plasmid (RC200477) and Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling Article Snippet: Mouse Nrp1 Plasmid , Origene , Cat# MR211197. .. Article Title: Glutaredoxin-1 attenuates transactive response DNA-binding protein 43–induced neurotoxicity by suppressing oxidative stress and transactive response DNA-binding protein 43 aggregation Article Snippet: Plasmids and siRNAs were transfected to cells using Lipofectamine 2000 (Invitrogen, Waltham, Massachusetts, USA) and Lipofectamine RNAiMAX (Invitrogen), respectively. .. The coding sequences of full-length human wild-type TDP-43 (hTDP-43 , NM_007375 ) and Grx1( NM_002064.3 ) were cloned into pAAV-MCS (VPK-410, Cell Biolabs, San Diego, California, USA) and Article Title: Glutaredoxin-1 attenuates transactive response DNA-binding protein 43–induced neurotoxicity by suppressing oxidative stress and transactive response DNA-binding protein 43 aggregation Article Snippet: Plasmids and siRNAs were transfected to cells using Lipofectamine 2000 (Invitrogen, Waltham, Massachusetts, USA) and Lipofectamine RNAiMAX (Invitrogen), respectively. .. The coding sequences of fulllength human wild-type TDP-43 (hTDP-43, NM_007375) and Grx1(NM_002064.3) were cloned into pAAV-MCS (VPK-410, Cell Biolabs, San Diego, California, USA) and Article Title: Dual-site phosphorylation of SLX4 stabilizes the SLX4–MUS81 interface to promote mitotic SMX assembly and genome protection Article Snippet: .. To construct plasmid pCMV6 Venus-3XFLAG-SLX4, Venus-3XFLAG-SLX4resist M1A was amplified with primers HDW0910 and HDW0911 and cloned into the SalI and PmeI sites of Cell Culture:Article Title: Combination of antibody-pyrrolobenzodiazepine derivative conjugate and PARP inhibitor Article Snippet: .. Suspension:Article Title: Combination of antibody-pyrrolobenzodiazepine derivative conjugate and PARP inhibitor Article Snippet: .. Immunocytochemistry:Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2. Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy. Flow Cytometry:Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2. Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy. Western Blot:Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2. Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy. Expressing:Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2. Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy. Construct:Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2. Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy. Article Title: Dual-site phosphorylation of SLX4 stabilizes the SLX4–MUS81 interface to promote mitotic SMX assembly and genome protection Article Snippet: .. To construct plasmid pCMV6 Venus-3XFLAG-SLX4, Venus-3XFLAG-SLX4resist M1A was amplified with primers HDW0910 and HDW0911 and cloned into the SalI and PmeI sites of Clone Assay:Article Title: Glutaredoxin-1 attenuates transactive response DNA-binding protein 43–induced neurotoxicity by suppressing oxidative stress and transactive response DNA-binding protein 43 aggregation Article Snippet: Plasmids and siRNAs were transfected to cells using Lipofectamine 2000 (Invitrogen, Waltham, Massachusetts, USA) and Lipofectamine RNAiMAX (Invitrogen), respectively. .. The coding sequences of full-length human wild-type TDP-43 (hTDP-43 , NM_007375 ) and Grx1( NM_002064.3 ) were cloned into pAAV-MCS (VPK-410, Cell Biolabs, San Diego, California, USA) and Article Title: Glutaredoxin-1 attenuates transactive response DNA-binding protein 43–induced neurotoxicity by suppressing oxidative stress and transactive response DNA-binding protein 43 aggregation Article Snippet: Plasmids and siRNAs were transfected to cells using Lipofectamine 2000 (Invitrogen, Waltham, Massachusetts, USA) and Lipofectamine RNAiMAX (Invitrogen), respectively. .. The coding sequences of fulllength human wild-type TDP-43 (hTDP-43, NM_007375) and Grx1(NM_002064.3) were cloned into pAAV-MCS (VPK-410, Cell Biolabs, San Diego, California, USA) and Article Title: Dual-site phosphorylation of SLX4 stabilizes the SLX4–MUS81 interface to promote mitotic SMX assembly and genome protection Article Snippet: .. To construct plasmid pCMV6 Venus-3XFLAG-SLX4, Venus-3XFLAG-SLX4resist M1A was amplified with primers HDW0910 and HDW0911 and cloned into the SalI and PmeI sites of Over Expression:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Other BGISEQ BGI Tech, Shenzhen, China N/A Agilent Seahorse XF24 bioanalyzer Agilent Technologies, CA, USA N/A Fluorescence microscope Olympus, Tokyo, Japan N/A iScience 29, 115950, June 19, 2026 e3 .. For stable overexpression of Aldh1a3, Plxnb1, or Nrp1, 908 or 399 cells were transfected with 2 μg of either mouse cDNA ORF clone or control ORF Transfection:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Other BGISEQ BGI Tech, Shenzhen, China N/A Agilent Seahorse XF24 bioanalyzer Agilent Technologies, CA, USA N/A Fluorescence microscope Olympus, Tokyo, Japan N/A iScience 29, 115950, June 19, 2026 e3 .. For stable overexpression of Aldh1a3, Plxnb1, or Nrp1, 908 or 399 cells were transfected with 2 μg of either mouse cDNA ORF clone or control ORF Control:Article Title: ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signaling. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Other BGISEQ BGI Tech, Shenzhen, China N/A Agilent Seahorse XF24 bioanalyzer Agilent Technologies, CA, USA N/A Fluorescence microscope Olympus, Tokyo, Japan N/A iScience 29, 115950, June 19, 2026 e3 .. For stable overexpression of Aldh1a3, Plxnb1, or Nrp1, 908 or 399 cells were transfected with 2 μg of either mouse cDNA ORF clone or control ORF Amplification:Article Title: Dual-site phosphorylation of SLX4 stabilizes the SLX4–MUS81 interface to promote mitotic SMX assembly and genome protection Article Snippet: .. To construct plasmid pCMV6 Venus-3XFLAG-SLX4, Venus-3XFLAG-SLX4resist M1A was amplified with primers HDW0910 and HDW0911 and cloned into the SalI and PmeI sites of Cloning:Article Title: Dual-site phosphorylation of SLX4 stabilizes the SLX4–MUS81 interface to promote mitotic SMX assembly and genome protection Article Snippet: .. To construct plasmid pCMV6 Venus-3XFLAG-SLX4, Venus-3XFLAG-SLX4resist M1A was amplified with primers HDW0910 and HDW0911 and cloned into the SalI and PmeI sites of |
