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luciferase validation vector ori empty plasmid  (Addgene inc)


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    Addgene inc luciferase validation vector ori empty plasmid
    Luciferase Validation Vector Ori Empty Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector/STARR-seq+luciferase+validation+vector_mP_empty+(Plasmid+%2399298)/pmc09712620-220-10-16
    Average 93 stars, based on 2 article reviews
    luciferase validation vector ori empty plasmid - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Amplification:

    Article Title: Estrogen Receptor 1 chromatin profiling in human breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions
    Article Snippet: For luciferase assays the regions of interest (WT) were PCR amplified from pooled male human genomic DNA (Promega). .. The amplified regions were cloned by Gibson assembly into a STARR luciferase vector ORI empty plasmid (Addgene #99298) (Muerdter et al. 2018) using the NEBuilder HiFi DNA Assembly master mix (NEB). ..

    Article Title: Estrogen receptor 1 chromatin profiling in human breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions.
    Article Snippet: breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions Stacey E.P.. Joosten, Sebastian Gregoricchio, Suzan Stelloo, Elif Yapıcı, Chia-Chi Flora Huang, Kerim Yavuz, Maria Donaldson Collier, Tunç Morova, Umut Berkay Altintas,̧ Yongsoo Kim, Sander Canisius, Cathy B. Moelans, Paul J. van Diest, Gozde Korkmaz, Nathan A.. Lack, Michiel Vermeulen, Sabine C. Linn, and Wilbert Zwart Division of Oncogenomics, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Oncode Institute, The Netherlands; Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen, 6500HB Nijmegen, The Netherlands; Koç University School of Medicine, 34450 Istanbul, Turkey; Koç University Research Center for Translational Medicine (KUTTAM), 34450 Istanbul, Turkey; Vancouver Prostate Centre, University of British Columbia, Vancouver, British Columbia, V6H 3Z6 Canada; Department of Pathology, Amsterdam University Medical Center, Cancer Center Amsterdam, 1081 HV Amsterdam, The Netherlands; Division of Molecular Pathology, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Department of Pathology, Utrecht University Medical Centre, 3584 CX Utrecht, The Netherlands; Division of Molecular Genetics, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Department of Medical Oncology, Antoni van Leeuwenhoek Hospital, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Department of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, The Netherlands

    Article Title: Extensive androgen receptor enhancer heterogeneity in primary prostate cancers underlies transcriptional diversity and metastatic potential
    Article Snippet: For performing luciferase assays, regions of interest were PCR amplified from pooled male human genomic DNA (Promega) with overhangs added for Gibson assembly. .. The amplified regions were then cloned into a modified STARR luciferase validation vector ORI empty plasmid (Addgene #99298) using the NEBuilder HiFi DNA Assembly master mix (NEB). ..

    Clone Assay:

    Article Title: Estrogen Receptor 1 chromatin profiling in human breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions
    Article Snippet: For luciferase assays the regions of interest (WT) were PCR amplified from pooled male human genomic DNA (Promega). .. The amplified regions were cloned by Gibson assembly into a STARR luciferase vector ORI empty plasmid (Addgene #99298) (Muerdter et al. 2018) using the NEBuilder HiFi DNA Assembly master mix (NEB). ..

    Article Title: Estrogen receptor 1 chromatin profiling in human breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions.
    Article Snippet: breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions Stacey E.P.. Joosten, Sebastian Gregoricchio, Suzan Stelloo, Elif Yapıcı, Chia-Chi Flora Huang, Kerim Yavuz, Maria Donaldson Collier, Tunç Morova, Umut Berkay Altintas,̧ Yongsoo Kim, Sander Canisius, Cathy B. Moelans, Paul J. van Diest, Gozde Korkmaz, Nathan A.. Lack, Michiel Vermeulen, Sabine C. Linn, and Wilbert Zwart Division of Oncogenomics, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Oncode Institute, The Netherlands; Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen, 6500HB Nijmegen, The Netherlands; Koç University School of Medicine, 34450 Istanbul, Turkey; Koç University Research Center for Translational Medicine (KUTTAM), 34450 Istanbul, Turkey; Vancouver Prostate Centre, University of British Columbia, Vancouver, British Columbia, V6H 3Z6 Canada; Department of Pathology, Amsterdam University Medical Center, Cancer Center Amsterdam, 1081 HV Amsterdam, The Netherlands; Division of Molecular Pathology, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Department of Pathology, Utrecht University Medical Centre, 3584 CX Utrecht, The Netherlands; Division of Molecular Genetics, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Department of Medical Oncology, Antoni van Leeuwenhoek Hospital, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Department of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, The Netherlands

    Article Title: Extensive androgen receptor enhancer heterogeneity in primary prostate cancers underlies transcriptional diversity and metastatic potential
    Article Snippet: For performing luciferase assays, regions of interest were PCR amplified from pooled male human genomic DNA (Promega) with overhangs added for Gibson assembly. .. The amplified regions were then cloned into a modified STARR luciferase validation vector ORI empty plasmid (Addgene #99298) using the NEBuilder HiFi DNA Assembly master mix (NEB). ..

    Luciferase:

    Article Title: Estrogen Receptor 1 chromatin profiling in human breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions
    Article Snippet: For luciferase assays the regions of interest (WT) were PCR amplified from pooled male human genomic DNA (Promega). .. The amplified regions were cloned by Gibson assembly into a STARR luciferase vector ORI empty plasmid (Addgene #99298) (Muerdter et al. 2018) using the NEBuilder HiFi DNA Assembly master mix (NEB). ..

    Article Title: Estrogen receptor 1 chromatin profiling in human breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions.
    Article Snippet: breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions Stacey E.P.. Joosten, Sebastian Gregoricchio, Suzan Stelloo, Elif Yapıcı, Chia-Chi Flora Huang, Kerim Yavuz, Maria Donaldson Collier, Tunç Morova, Umut Berkay Altintas,̧ Yongsoo Kim, Sander Canisius, Cathy B. Moelans, Paul J. van Diest, Gozde Korkmaz, Nathan A.. Lack, Michiel Vermeulen, Sabine C. Linn, and Wilbert Zwart Division of Oncogenomics, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Oncode Institute, The Netherlands; Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen, 6500HB Nijmegen, The Netherlands; Koç University School of Medicine, 34450 Istanbul, Turkey; Koç University Research Center for Translational Medicine (KUTTAM), 34450 Istanbul, Turkey; Vancouver Prostate Centre, University of British Columbia, Vancouver, British Columbia, V6H 3Z6 Canada; Department of Pathology, Amsterdam University Medical Center, Cancer Center Amsterdam, 1081 HV Amsterdam, The Netherlands; Division of Molecular Pathology, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Department of Pathology, Utrecht University Medical Centre, 3584 CX Utrecht, The Netherlands; Division of Molecular Genetics, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Department of Medical Oncology, Antoni van Leeuwenhoek Hospital, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Department of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, The Netherlands

    Article Title: Extensive androgen receptor enhancer heterogeneity in primary prostate cancers underlies transcriptional diversity and metastatic potential
    Article Snippet: For performing luciferase assays, regions of interest were PCR amplified from pooled male human genomic DNA (Promega) with overhangs added for Gibson assembly. .. The amplified regions were then cloned into a modified STARR luciferase validation vector ORI empty plasmid (Addgene #99298) using the NEBuilder HiFi DNA Assembly master mix (NEB). ..

    Plasmid Preparation:

    Article Title: Estrogen Receptor 1 chromatin profiling in human breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions
    Article Snippet: For luciferase assays the regions of interest (WT) were PCR amplified from pooled male human genomic DNA (Promega). .. The amplified regions were cloned by Gibson assembly into a STARR luciferase vector ORI empty plasmid (Addgene #99298) (Muerdter et al. 2018) using the NEBuilder HiFi DNA Assembly master mix (NEB). ..

    Article Title: Estrogen receptor 1 chromatin profiling in human breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions.
    Article Snippet: breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions Stacey E.P.. Joosten, Sebastian Gregoricchio, Suzan Stelloo, Elif Yapıcı, Chia-Chi Flora Huang, Kerim Yavuz, Maria Donaldson Collier, Tunç Morova, Umut Berkay Altintas,̧ Yongsoo Kim, Sander Canisius, Cathy B. Moelans, Paul J. van Diest, Gozde Korkmaz, Nathan A.. Lack, Michiel Vermeulen, Sabine C. Linn, and Wilbert Zwart Division of Oncogenomics, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Oncode Institute, The Netherlands; Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen, 6500HB Nijmegen, The Netherlands; Koç University School of Medicine, 34450 Istanbul, Turkey; Koç University Research Center for Translational Medicine (KUTTAM), 34450 Istanbul, Turkey; Vancouver Prostate Centre, University of British Columbia, Vancouver, British Columbia, V6H 3Z6 Canada; Department of Pathology, Amsterdam University Medical Center, Cancer Center Amsterdam, 1081 HV Amsterdam, The Netherlands; Division of Molecular Pathology, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Department of Pathology, Utrecht University Medical Centre, 3584 CX Utrecht, The Netherlands; Division of Molecular Genetics, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Department of Medical Oncology, Antoni van Leeuwenhoek Hospital, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Department of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, The Netherlands

    Article Title: Extensive androgen receptor enhancer heterogeneity in primary prostate cancers underlies transcriptional diversity and metastatic potential
    Article Snippet: For performing luciferase assays, regions of interest were PCR amplified from pooled male human genomic DNA (Promega) with overhangs added for Gibson assembly. .. The amplified regions were then cloned into a modified STARR luciferase validation vector ORI empty plasmid (Addgene #99298) using the NEBuilder HiFi DNA Assembly master mix (NEB). ..

    Modification:

    Article Title: Extensive androgen receptor enhancer heterogeneity in primary prostate cancers underlies transcriptional diversity and metastatic potential
    Article Snippet: For performing luciferase assays, regions of interest were PCR amplified from pooled male human genomic DNA (Promega) with overhangs added for Gibson assembly. .. The amplified regions were then cloned into a modified STARR luciferase validation vector ORI empty plasmid (Addgene #99298) using the NEBuilder HiFi DNA Assembly master mix (NEB). ..

    Biomarker Discovery:

    Article Title: Extensive androgen receptor enhancer heterogeneity in primary prostate cancers underlies transcriptional diversity and metastatic potential
    Article Snippet: For performing luciferase assays, regions of interest were PCR amplified from pooled male human genomic DNA (Promega) with overhangs added for Gibson assembly. .. The amplified regions were then cloned into a modified STARR luciferase validation vector ORI empty plasmid (Addgene #99298) using the NEBuilder HiFi DNA Assembly master mix (NEB). ..



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    Image Search Results


    a, HCT116 cells stably expressing BRIP1 R162Q were transiently transfected using PEI with empty vector (EV-EGFP) or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.

    Journal: bioRxiv

    Article Title: Role of a childhood cancer-linked BRIP1/FANCJ germline variant in genomic instability and cancer cell vulnerability

    doi: 10.64898/2026.03.24.714005

    Figure Lengend Snippet: a, HCT116 cells stably expressing BRIP1 R162Q were transiently transfected using PEI with empty vector (EV-EGFP) or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.

    Article Snippet: For R-loop removal, cells were transiently transfected with an EGFP empty vector control (pEGFP-C1, Takara Bio, #632470), EGFP-tagged RNaseH1 wild-type (WT), or the catalytically inactive RNaseH1 D145N mutant as indicated.

    Techniques: Stable Transfection, Expressing, Transfection, Plasmid Preparation, Fluorescence, Western Blot, Construct, Immunofluorescence, Labeling, Confocal Microscopy, Two Tailed Test