luciferase validation vector ori empty plasmid (Addgene inc)
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Luciferase Validation Vector Ori Empty Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+vector/STARR-seq+luciferase+validation+vector_mP_empty+(Plasmid+%2399298)/pmc09712620-220-10-16
Average 93 stars, based on 2 article reviews
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Amplification:Article Title: Estrogen Receptor 1 chromatin profiling in human breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions Article Snippet: For luciferase assays the regions of interest (WT) were PCR amplified from pooled male human genomic DNA (Promega). .. The amplified regions were cloned by Gibson assembly into a STARR Article Title: Estrogen receptor 1 chromatin profiling in human breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions. Article Snippet: breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions Stacey E.P.. Joosten, Sebastian Gregoricchio, Suzan Stelloo, Elif Yapıcı, Chia-Chi Flora Huang, Kerim Yavuz, Maria Donaldson Collier, Tunç Morova, Umut Berkay Altintas,̧ Yongsoo Kim, Sander Canisius, Cathy B. Moelans, Paul J. van Diest, Gozde Korkmaz, Nathan A.. Lack, Michiel Vermeulen, Sabine C. Linn, and Wilbert Zwart Division of Oncogenomics, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Oncode Institute, The Netherlands; Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen, 6500HB Nijmegen, The Netherlands; Koç University School of Medicine, 34450 Istanbul, Turkey; Koç University Research Center for Translational Medicine (KUTTAM), 34450 Istanbul, Turkey; Vancouver Prostate Centre, University of British Columbia, Vancouver, British Columbia, V6H 3Z6 Canada; Department of Pathology, Amsterdam University Medical Center, Cancer Center Amsterdam, 1081 HV Amsterdam, The Netherlands; Division of Molecular Pathology, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Department of Pathology, Utrecht University Medical Centre, 3584 CX Utrecht, The Netherlands; Division of Molecular Genetics, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Department of Medical Oncology, Antoni van Leeuwenhoek Hospital, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Department of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, The Netherlands Article Title: Extensive androgen receptor enhancer heterogeneity in primary prostate cancers underlies transcriptional diversity and metastatic potential Article Snippet: For performing luciferase assays, regions of interest were PCR amplified from pooled male human genomic DNA (Promega) with overhangs added for Gibson assembly. .. The amplified regions were then cloned into a modified STARR Clone Assay:Article Title: Estrogen Receptor 1 chromatin profiling in human breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions Article Snippet: For luciferase assays the regions of interest (WT) were PCR amplified from pooled male human genomic DNA (Promega). .. The amplified regions were cloned by Gibson assembly into a STARR Article Title: Estrogen receptor 1 chromatin profiling in human breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions. Article Snippet: breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions Stacey E.P.. Joosten, Sebastian Gregoricchio, Suzan Stelloo, Elif Yapıcı, Chia-Chi Flora Huang, Kerim Yavuz, Maria Donaldson Collier, Tunç Morova, Umut Berkay Altintas,̧ Yongsoo Kim, Sander Canisius, Cathy B. Moelans, Paul J. van Diest, Gozde Korkmaz, Nathan A.. Lack, Michiel Vermeulen, Sabine C. Linn, and Wilbert Zwart Division of Oncogenomics, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Oncode Institute, The Netherlands; Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen, 6500HB Nijmegen, The Netherlands; Koç University School of Medicine, 34450 Istanbul, Turkey; Koç University Research Center for Translational Medicine (KUTTAM), 34450 Istanbul, Turkey; Vancouver Prostate Centre, University of British Columbia, Vancouver, British Columbia, V6H 3Z6 Canada; Department of Pathology, Amsterdam University Medical Center, Cancer Center Amsterdam, 1081 HV Amsterdam, The Netherlands; Division of Molecular Pathology, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Department of Pathology, Utrecht University Medical Centre, 3584 CX Utrecht, The Netherlands; Division of Molecular Genetics, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Department of Medical Oncology, Antoni van Leeuwenhoek Hospital, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Department of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, The Netherlands Article Title: Extensive androgen receptor enhancer heterogeneity in primary prostate cancers underlies transcriptional diversity and metastatic potential Article Snippet: For performing luciferase assays, regions of interest were PCR amplified from pooled male human genomic DNA (Promega) with overhangs added for Gibson assembly. .. The amplified regions were then cloned into a modified STARR Luciferase:Article Title: Estrogen Receptor 1 chromatin profiling in human breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions Article Snippet: For luciferase assays the regions of interest (WT) were PCR amplified from pooled male human genomic DNA (Promega). .. The amplified regions were cloned by Gibson assembly into a STARR Article Title: Estrogen receptor 1 chromatin profiling in human breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions. Article Snippet: breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions Stacey E.P.. Joosten, Sebastian Gregoricchio, Suzan Stelloo, Elif Yapıcı, Chia-Chi Flora Huang, Kerim Yavuz, Maria Donaldson Collier, Tunç Morova, Umut Berkay Altintas,̧ Yongsoo Kim, Sander Canisius, Cathy B. Moelans, Paul J. van Diest, Gozde Korkmaz, Nathan A.. Lack, Michiel Vermeulen, Sabine C. Linn, and Wilbert Zwart Division of Oncogenomics, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Oncode Institute, The Netherlands; Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen, 6500HB Nijmegen, The Netherlands; Koç University School of Medicine, 34450 Istanbul, Turkey; Koç University Research Center for Translational Medicine (KUTTAM), 34450 Istanbul, Turkey; Vancouver Prostate Centre, University of British Columbia, Vancouver, British Columbia, V6H 3Z6 Canada; Department of Pathology, Amsterdam University Medical Center, Cancer Center Amsterdam, 1081 HV Amsterdam, The Netherlands; Division of Molecular Pathology, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Department of Pathology, Utrecht University Medical Centre, 3584 CX Utrecht, The Netherlands; Division of Molecular Genetics, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Department of Medical Oncology, Antoni van Leeuwenhoek Hospital, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Department of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, The Netherlands Article Title: Extensive androgen receptor enhancer heterogeneity in primary prostate cancers underlies transcriptional diversity and metastatic potential Article Snippet: For performing luciferase assays, regions of interest were PCR amplified from pooled male human genomic DNA (Promega) with overhangs added for Gibson assembly. .. The amplified regions were then cloned into a modified STARR Plasmid Preparation:Article Title: Estrogen Receptor 1 chromatin profiling in human breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions Article Snippet: For luciferase assays the regions of interest (WT) were PCR amplified from pooled male human genomic DNA (Promega). .. The amplified regions were cloned by Gibson assembly into a STARR Article Title: Estrogen receptor 1 chromatin profiling in human breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions. Article Snippet: breast tumors reveals high inter-patient heterogeneity with enrichment of risk SNPs and enhancer activity at most-conserved regions Stacey E.P.. Joosten, Sebastian Gregoricchio, Suzan Stelloo, Elif Yapıcı, Chia-Chi Flora Huang, Kerim Yavuz, Maria Donaldson Collier, Tunç Morova, Umut Berkay Altintas,̧ Yongsoo Kim, Sander Canisius, Cathy B. Moelans, Paul J. van Diest, Gozde Korkmaz, Nathan A.. Lack, Michiel Vermeulen, Sabine C. Linn, and Wilbert Zwart Division of Oncogenomics, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Oncode Institute, The Netherlands; Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Radboud University Nijmegen, 6500HB Nijmegen, The Netherlands; Koç University School of Medicine, 34450 Istanbul, Turkey; Koç University Research Center for Translational Medicine (KUTTAM), 34450 Istanbul, Turkey; Vancouver Prostate Centre, University of British Columbia, Vancouver, British Columbia, V6H 3Z6 Canada; Department of Pathology, Amsterdam University Medical Center, Cancer Center Amsterdam, 1081 HV Amsterdam, The Netherlands; Division of Molecular Pathology, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Department of Pathology, Utrecht University Medical Centre, 3584 CX Utrecht, The Netherlands; Division of Molecular Genetics, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Department of Medical Oncology, Antoni van Leeuwenhoek Hospital, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands; Department of Biomedical Engineering, Eindhoven University of Technology, 5600 MB Eindhoven, The Netherlands Article Title: Extensive androgen receptor enhancer heterogeneity in primary prostate cancers underlies transcriptional diversity and metastatic potential Article Snippet: For performing luciferase assays, regions of interest were PCR amplified from pooled male human genomic DNA (Promega) with overhangs added for Gibson assembly. .. The amplified regions were then cloned into a modified STARR Modification:Article Title: Extensive androgen receptor enhancer heterogeneity in primary prostate cancers underlies transcriptional diversity and metastatic potential Article Snippet: For performing luciferase assays, regions of interest were PCR amplified from pooled male human genomic DNA (Promega) with overhangs added for Gibson assembly. .. The amplified regions were then cloned into a modified STARR Biomarker Discovery:Article Title: Extensive androgen receptor enhancer heterogeneity in primary prostate cancers underlies transcriptional diversity and metastatic potential Article Snippet: For performing luciferase assays, regions of interest were PCR amplified from pooled male human genomic DNA (Promega) with overhangs added for Gibson assembly. .. The amplified regions were then cloned into a modified STARR |
