empty pgadt7 vector (TaKaRa)
TaKaRa is a verified supplier 
TaKaRa manufactures this product 
99
Structured Review
TaKaRa
empty pgadt7 vector
Empty Pgadt7 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 4006 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+vector+pgadt7/pGADT7+AD+Vector/pmc13161086-261-5-36
Average 99 stars, based on 4006 article reviews
Empty Pgadt7 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 4006 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+vector+pgadt7/pGADT7+AD+Vector/pmc13161086-261-5-36
Average 99 stars, based on 4006 article reviews
empty pgadt7 vector - by Bioz Stars,
2026-11
99/100 stars
Images
Related Articles
cDNA Library Assay:Article Title: Screening human brain targets for Japanese encephalitis virus NS5 via yeast two-hybrid assay. Article Snippet: The recombinant pGBKT7-JEV NS5 and the empty pGBKT7 plasmids were transformed into the Y2HGold yeast strain using a highefficiency polyethylene glycol (PEG)/LiAc-based method (Clontech, USA) and spread on the following plates; minimal yeast medium without tryptophan (SD/-Trp), SD/-Trp supplemented with 40 μg/mL X-alpha-Gal (SD/-Trp/X), and SD/-Trp supplemented with 40 μg/mL X-alpha-Gal and 125 ng/mL Aureobasidin A (SD/-Trp/X/A) and incubated for 3-5 days. .. Preparation of human brain cDNA library strain Human brain prey coding sequences were recombined into Article Title: Daam-, FMNL-, and mDia-family formins jointly regulate cortical actin dynamics in melanoma cells. Article Snippet: .. The cDNAs encoding murine FHOD1-GBD/FH3 (Uniprot ID:Q6P9Q4, residues 53–462), FMNL2-GBD/FH3 (Uniprot ID:A2APV2-3, residues 23–469), FMNL3-GBD/FH3 (Uniprot ID:Q6ZPF4-1, residues 26–472), and Daam1-GBD/FH3 (Uniprot ID:Q8BPM0-2, residues 45–420) were amplified from the B16-F1 cDNA library and inserted into the BamHI and XhoI sites of Article Title: A novel CsRNF‐CsHSF24‐ CsF3′5′H2 module regulates epigallocatechin gallate biosynthesis in Camellia sinensis Article Snippet: .. A cDNA library was constructed from tea plant (FDDB) leaf mRNA in the Activation Assay:Article Title: Screening human brain targets for Japanese encephalitis virus NS5 via yeast two-hybrid assay. Article Snippet: The recombinant pGBKT7-JEV NS5 and the empty pGBKT7 plasmids were transformed into the Y2HGold yeast strain using a highefficiency polyethylene glycol (PEG)/LiAc-based method (Clontech, USA) and spread on the following plates; minimal yeast medium without tryptophan (SD/-Trp), SD/-Trp supplemented with 40 μg/mL X-alpha-Gal (SD/-Trp/X), and SD/-Trp supplemented with 40 μg/mL X-alpha-Gal and 125 ng/mL Aureobasidin A (SD/-Trp/X/A) and incubated for 3-5 days. .. Preparation of human brain cDNA library strain Human brain prey coding sequences were recombined into Article Title: Three PRR proteins interact with ARF10 and ARF16 to enhance ABI5 activation and auxin-stimulated ABA responses during Arabidopsis seed germination. Article Snippet: 950 951 Y2H assays 952 The full-length PRR5 CDS were inserted into pGBKT7 vector (Clontech) to produce 953 the bait vector (BD-PRR5) containing the Gal4 DNA-binding domain. .. The full-length 954 ARF16 CDS were incorporated into Article Title: WSSV355 enhances IDH1 enzymatic activity and promotes efficient WSSV replication. Article Snippet: Jo ur na l P re -p ro of Journal Pre-proof 2 White spot syndrome virus (WSSV) drives extensive metabolic reprogramming to support viral replication, including activation of reductive carboxylation, a pathway in which host isocitrate dehydrogenase 1 (IDH1) has a central role.. However, the mechanisms by which viral factors regulate IDH1 and associated metabolic networks remain unclear.. Here, we identified WSSV355 as a key viral regulator that contributed to IDH1 binding and activation during infection. Transformation Assay:Article Title: Screening human brain targets for Japanese encephalitis virus NS5 via yeast two-hybrid assay. Article Snippet: The recombinant pGBKT7-JEV NS5 and the empty pGBKT7 plasmids were transformed into the Y2HGold yeast strain using a highefficiency polyethylene glycol (PEG)/LiAc-based method (Clontech, USA) and spread on the following plates; minimal yeast medium without tryptophan (SD/-Trp), SD/-Trp supplemented with 40 μg/mL X-alpha-Gal (SD/-Trp/X), and SD/-Trp supplemented with 40 μg/mL X-alpha-Gal and 125 ng/mL Aureobasidin A (SD/-Trp/X/A) and incubated for 3-5 days. .. Preparation of human brain cDNA library strain Human brain prey coding sequences were recombined into Article Title: Phosphorylation of the plant photoreceptor UVR8 regulates its dimer/monomer status and enhances UV-B signaling across species. Article Snippet: Yeast two-hybrid and three-hybrid assays The UVR8, UVR8T356A, and UVR8T356D coding sequences were cloned into pGBKT7 and transformed into the Y2H Gold strain (Clontech). .. The UVR8, UVR8T356A, UVR8T356D, COP1, and RUP2 sequences were cloned into Yeast:Article Title: Screening human brain targets for Japanese encephalitis virus NS5 via yeast two-hybrid assay. Article Snippet: The recombinant pGBKT7-JEV NS5 and the empty pGBKT7 plasmids were transformed into the Y2HGold yeast strain using a highefficiency polyethylene glycol (PEG)/LiAc-based method (Clontech, USA) and spread on the following plates; minimal yeast medium without tryptophan (SD/-Trp), SD/-Trp supplemented with 40 μg/mL X-alpha-Gal (SD/-Trp/X), and SD/-Trp supplemented with 40 μg/mL X-alpha-Gal and 125 ng/mL Aureobasidin A (SD/-Trp/X/A) and incubated for 3-5 days. .. Preparation of human brain cDNA library strain Human brain prey coding sequences were recombined into Article Title: Identification of GEI-14 as a candidate pro-crossover factor by TurboID in C. elegans Article Snippet: .. For yeast two-hybrid assays, full-length coding sequences for GEI-14 and known pro-crossover factors ( CDK-2 , COSA-1 /COSA-1-6A, MSH-4, MSH-5 , ZHP-3 ) were cloned into Plasmid Preparation:Article Title: Three PRR proteins interact with ARF10 and ARF16 to enhance ABI5 activation and auxin-stimulated ABA responses during Arabidopsis seed germination. Article Snippet: 950 951 Y2H assays 952 The full-length PRR5 CDS were inserted into pGBKT7 vector (Clontech) to produce 953 the bait vector (BD-PRR5) containing the Gal4 DNA-binding domain. .. The full-length 954 ARF16 CDS were incorporated into Article Title: WSSV355 enhances IDH1 enzymatic activity and promotes efficient WSSV replication. Article Snippet: Jo ur na l P re -p ro of Journal Pre-proof 2 White spot syndrome virus (WSSV) drives extensive metabolic reprogramming to support viral replication, including activation of reductive carboxylation, a pathway in which host isocitrate dehydrogenase 1 (IDH1) has a central role.. However, the mechanisms by which viral factors regulate IDH1 and associated metabolic networks remain unclear.. Here, we identified WSSV355 as a key viral regulator that contributed to IDH1 binding and activation during infection. Article Title: Identification of GEI-14 as a candidate pro-crossover factor by TurboID in C. elegans Article Snippet: .. For yeast two-hybrid assays, full-length coding sequences for GEI-14 and known pro-crossover factors ( CDK-2 , COSA-1 /COSA-1-6A, MSH-4, MSH-5 , ZHP-3 ) were cloned into Article Title: MdAGL29 positively regulates MdBRC1/2 and negatively controls shoot branching in apple. Article Snippet: Key message The MADS-box transcription factor MdAGL29 serves as a negative regulator of branching in apple by modulating MdBRC1/2 expression.. Abstract Branch number is one of the major determinants in apple tree architecture.. MdAGL29, a member of the MADS-box transcription factor family, is downregulated by 6-benzylaminopurine, a synthetic cytokinin. Article Title: Daam-, FMNL-, and mDia-family formins jointly regulate cortical actin dynamics in melanoma cells. Article Snippet: .. The cDNAs encoding murine FHOD1-GBD/FH3 (Uniprot ID:Q6P9Q4, residues 53–462), FMNL2-GBD/FH3 (Uniprot ID:A2APV2-3, residues 23–469), FMNL3-GBD/FH3 (Uniprot ID:Q6ZPF4-1, residues 26–472), and Daam1-GBD/FH3 (Uniprot ID:Q8BPM0-2, residues 45–420) were amplified from the B16-F1 cDNA library and inserted into the BamHI and XhoI sites of Article Title: A novel CsRNF‐CsHSF24‐ CsF3′5′H2 module regulates epigallocatechin gallate biosynthesis in Camellia sinensis Article Snippet: .. A cDNA library was constructed from tea plant (FDDB) leaf mRNA in the Construct:Article Title: WSSV355 enhances IDH1 enzymatic activity and promotes efficient WSSV replication. Article Snippet: Jo ur na l P re -p ro of Journal Pre-proof 2 White spot syndrome virus (WSSV) drives extensive metabolic reprogramming to support viral replication, including activation of reductive carboxylation, a pathway in which host isocitrate dehydrogenase 1 (IDH1) has a central role.. However, the mechanisms by which viral factors regulate IDH1 and associated metabolic networks remain unclear.. Here, we identified WSSV355 as a key viral regulator that contributed to IDH1 binding and activation during infection. Article Title: MdAGL29 positively regulates MdBRC1/2 and negatively controls shoot branching in apple. Article Snippet: Key message The MADS-box transcription factor MdAGL29 serves as a negative regulator of branching in apple by modulating MdBRC1/2 expression.. Abstract Branch number is one of the major determinants in apple tree architecture.. MdAGL29, a member of the MADS-box transcription factor family, is downregulated by 6-benzylaminopurine, a synthetic cytokinin. Article Title: A novel CsRNF‐CsHSF24‐ CsF3′5′H2 module regulates epigallocatechin gallate biosynthesis in Camellia sinensis Article Snippet: .. A cDNA library was constructed from tea plant (FDDB) leaf mRNA in the Clone Assay:Article Title: Identification of GEI-14 as a candidate pro-crossover factor by TurboID in C. elegans Article Snippet: .. For yeast two-hybrid assays, full-length coding sequences for GEI-14 and known pro-crossover factors ( CDK-2 , COSA-1 /COSA-1-6A, MSH-4, MSH-5 , ZHP-3 ) were cloned into Article Title: MdAGL29 positively regulates MdBRC1/2 and negatively controls shoot branching in apple. Article Snippet: Key message The MADS-box transcription factor MdAGL29 serves as a negative regulator of branching in apple by modulating MdBRC1/2 expression.. Abstract Branch number is one of the major determinants in apple tree architecture.. MdAGL29, a member of the MADS-box transcription factor family, is downregulated by 6-benzylaminopurine, a synthetic cytokinin. Article Title: Phosphorylation of the plant photoreceptor UVR8 regulates its dimer/monomer status and enhances UV-B signaling across species. Article Snippet: Yeast two-hybrid and three-hybrid assays The UVR8, UVR8T356A, and UVR8T356D coding sequences were cloned into pGBKT7 and transformed into the Y2H Gold strain (Clontech). .. The UVR8, UVR8T356A, UVR8T356D, COP1, and RUP2 sequences were cloned into Sequencing:Article Title: MdAGL29 positively regulates MdBRC1/2 and negatively controls shoot branching in apple. Article Snippet: Key message The MADS-box transcription factor MdAGL29 serves as a negative regulator of branching in apple by modulating MdBRC1/2 expression.. Abstract Branch number is one of the major determinants in apple tree architecture.. MdAGL29, a member of the MADS-box transcription factor family, is downregulated by 6-benzylaminopurine, a synthetic cytokinin. Amplification:Article Title: MdAGL29 positively regulates MdBRC1/2 and negatively controls shoot branching in apple. Article Snippet: Key message The MADS-box transcription factor MdAGL29 serves as a negative regulator of branching in apple by modulating MdBRC1/2 expression.. Abstract Branch number is one of the major determinants in apple tree architecture.. MdAGL29, a member of the MADS-box transcription factor family, is downregulated by 6-benzylaminopurine, a synthetic cytokinin. Article Title: Daam-, FMNL-, and mDia-family formins jointly regulate cortical actin dynamics in melanoma cells. Article Snippet: .. The cDNAs encoding murine FHOD1-GBD/FH3 (Uniprot ID:Q6P9Q4, residues 53–462), FMNL2-GBD/FH3 (Uniprot ID:A2APV2-3, residues 23–469), FMNL3-GBD/FH3 (Uniprot ID:Q6ZPF4-1, residues 26–472), and Daam1-GBD/FH3 (Uniprot ID:Q8BPM0-2, residues 45–420) were amplified from the B16-F1 cDNA library and inserted into the BamHI and XhoI sites of |
