plko.1-puro plasmid (Merck KGaA)
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Plko.1 Puro Plasmid, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+vector+control/plko+1+puro+empty+vector+control+transduction+particles/pm33968201-91-3-17
Average 90 stars, based on 1 article reviews
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Control:Article Title: All-trans retinoic acid-mediated ADAR1 degradation synergizes with PD-1 blockade to suppress pancreatic cancer Article Snippet: The pLKO.1-shLuc control plasmid (TRCN0000072243), pLKO.1-shLacZ control plasmid (TRCN0000072237), and ADAR short hairpin RNAs (TRCN0000301036 and TRCN0000050788), used for Article Title: Interactions of Antibodies to the Gram-Negative Gastric Bacterium Helicobacter pylori with the Synaptic Calcium Sensor Synaptotagmin 5, Correlate to Impaired Vesicle Recycling in SiMa Human Neuroblastoma Cells. Article Snippet: For Article Title: SPARC promotes pancreatic cancer cell proliferation and migration through autocrine secretion into the extracellular milieu. Article Snippet: The sequence of siRNA1 was used for the construction of SPARC shRNA using a pLKO.1‐puro plasmid (Sigma‐Aldrich; Merck KGaA). Article Title: ALDH1A1 confers resistance to RAF/MEK inhibitors in melanoma cells by maintaining stemness phenotype and activating PI3K/AKT signaling Article Snippet: Page 5/23 To achieve a stable ALDH1A1 knockdown (ALDH1A1KD), 1.5×105 melanoma cells were seeded on 6- multiplates and transduced at 70% Article Title: ALDH1A1 overexpression in melanoma cells promotes tumor angiogenesis by activating the IL-8/Notch signaling cascade Article Snippet: To achieve a stable knockdown, 1.5×10 5 melanoma cells were seeded on 6-multiplates and transduced at 70% confluence with Article Title: ALDH1A1 confers resistance to RAF/MEK inhibitors in melanoma cells by maintaining stemness phenotype and activating PI3K/AKT signaling. Article Snippet: When 70 % of confluence was reached, the Article Title: Interactions of Antibodies to the Gram-Negative Gastric Bacterium Helicobacter pylori with the Synaptic Calcium Sensor Synaptotagmin 5, Correlate to Impaired Vesicle Recycling in SiMa Human Neuroblastoma Cells Article Snippet: For Plasmid Preparation:Article Title: All-trans retinoic acid-mediated ADAR1 degradation synergizes with PD-1 blockade to suppress pancreatic cancer Article Snippet: The pLKO.1-shLuc control plasmid (TRCN0000072243), pLKO.1-shLacZ control plasmid (TRCN0000072237), and ADAR short hairpin RNAs (TRCN0000301036 and TRCN0000050788), used for Article Title: Interactions of Antibodies to the Gram-Negative Gastric Bacterium Helicobacter pylori with the Synaptic Calcium Sensor Synaptotagmin 5, Correlate to Impaired Vesicle Recycling in SiMa Human Neuroblastoma Cells. Article Snippet: For Article Title: SPARC promotes pancreatic cancer cell proliferation and migration through autocrine secretion into the extracellular milieu. Article Snippet: The sequence of siRNA1 was used for the construction of SPARC shRNA using a pLKO.1‐puro plasmid (Sigma‐Aldrich; Merck KGaA). Article Title: ALDH1A1 confers resistance to RAF/MEK inhibitors in melanoma cells by maintaining stemness phenotype and activating PI3K/AKT signaling Article Snippet: Page 5/23 To achieve a stable ALDH1A1 knockdown (ALDH1A1KD), 1.5×105 melanoma cells were seeded on 6- multiplates and transduced at 70% Article Title: ALDH1A1 overexpression in melanoma cells promotes tumor angiogenesis by activating the IL-8/Notch signaling cascade Article Snippet: To achieve a stable knockdown, 1.5×10 5 melanoma cells were seeded on 6-multiplates and transduced at 70% confluence with Article Title: ALDH1A1 confers resistance to RAF/MEK inhibitors in melanoma cells by maintaining stemness phenotype and activating PI3K/AKT signaling. Article Snippet: When 70 % of confluence was reached, the Article Title: Interactions of Antibodies to the Gram-Negative Gastric Bacterium Helicobacter pylori with the Synaptic Calcium Sensor Synaptotagmin 5, Correlate to Impaired Vesicle Recycling in SiMa Human Neuroblastoma Cells Article Snippet: For Transduction:Article Title: All-trans retinoic acid-mediated ADAR1 degradation synergizes with PD-1 blockade to suppress pancreatic cancer Article Snippet: The pLKO.1-shLuc control plasmid (TRCN0000072243), pLKO.1-shLacZ control plasmid (TRCN0000072237), and ADAR short hairpin RNAs (TRCN0000301036 and TRCN0000050788), used for Article Title: Interactions of Antibodies to the Gram-Negative Gastric Bacterium Helicobacter pylori with the Synaptic Calcium Sensor Synaptotagmin 5, Correlate to Impaired Vesicle Recycling in SiMa Human Neuroblastoma Cells. Article Snippet: For Article Title: SPARC promotes pancreatic cancer cell proliferation and migration through autocrine secretion into the extracellular milieu. Article Snippet: The sequence of siRNA1 was used for the construction of SPARC shRNA using a pLKO.1‐puro plasmid (Sigma‐Aldrich; Merck KGaA). Article Title: ALDH1A1 confers resistance to RAF/MEK inhibitors in melanoma cells by maintaining stemness phenotype and activating PI3K/AKT signaling Article Snippet: Page 5/23 To achieve a stable ALDH1A1 knockdown (ALDH1A1KD), 1.5×105 melanoma cells were seeded on 6- multiplates and transduced at 70% Article Title: ALDH1A1 overexpression in melanoma cells promotes tumor angiogenesis by activating the IL-8/Notch signaling cascade Article Snippet: To achieve a stable knockdown, 1.5×10 5 melanoma cells were seeded on 6-multiplates and transduced at 70% confluence with Article Title: ALDH1A1 confers resistance to RAF/MEK inhibitors in melanoma cells by maintaining stemness phenotype and activating PI3K/AKT signaling. Article Snippet: When 70 % of confluence was reached, the Article Title: Interactions of Antibodies to the Gram-Negative Gastric Bacterium Helicobacter pylori with the Synaptic Calcium Sensor Synaptotagmin 5, Correlate to Impaired Vesicle Recycling in SiMa Human Neuroblastoma Cells Article Snippet: For Expressing:Article Title: All-trans retinoic acid-mediated ADAR1 degradation synergizes with PD-1 blockade to suppress pancreatic cancer Article Snippet: The pLKO.1-shLuc control plasmid (TRCN0000072243), pLKO.1-shLacZ control plasmid (TRCN0000072237), and ADAR short hairpin RNAs (TRCN0000301036 and TRCN0000050788), used for Article Title: Interactions of Antibodies to the Gram-Negative Gastric Bacterium Helicobacter pylori with the Synaptic Calcium Sensor Synaptotagmin 5, Correlate to Impaired Vesicle Recycling in SiMa Human Neuroblastoma Cells. Article Snippet: For Article Title: SPARC promotes pancreatic cancer cell proliferation and migration through autocrine secretion into the extracellular milieu. Article Snippet: The sequence of siRNA1 was used for the construction of SPARC shRNA using a pLKO.1‐puro plasmid (Sigma‐Aldrich; Merck KGaA). Article Title: ALDH1A1 confers resistance to RAF/MEK inhibitors in melanoma cells by maintaining stemness phenotype and activating PI3K/AKT signaling Article Snippet: Page 5/23 To achieve a stable ALDH1A1 knockdown (ALDH1A1KD), 1.5×105 melanoma cells were seeded on 6- multiplates and transduced at 70% Article Title: ALDH1A1 overexpression in melanoma cells promotes tumor angiogenesis by activating the IL-8/Notch signaling cascade Article Snippet: To achieve a stable knockdown, 1.5×10 5 melanoma cells were seeded on 6-multiplates and transduced at 70% confluence with Article Title: ALDH1A1 confers resistance to RAF/MEK inhibitors in melanoma cells by maintaining stemness phenotype and activating PI3K/AKT signaling. Article Snippet: When 70 % of confluence was reached, the Article Title: Interactions of Antibodies to the Gram-Negative Gastric Bacterium Helicobacter pylori with the Synaptic Calcium Sensor Synaptotagmin 5, Correlate to Impaired Vesicle Recycling in SiMa Human Neuroblastoma Cells Article Snippet: For Incubation:Article Title: All-trans retinoic acid-mediated ADAR1 degradation synergizes with PD-1 blockade to suppress pancreatic cancer Article Snippet: The pLKO.1-shLuc control plasmid (TRCN0000072243), pLKO.1-shLacZ control plasmid (TRCN0000072237), and ADAR short hairpin RNAs (TRCN0000301036 and TRCN0000050788), used for Article Title: Interactions of Antibodies to the Gram-Negative Gastric Bacterium Helicobacter pylori with the Synaptic Calcium Sensor Synaptotagmin 5, Correlate to Impaired Vesicle Recycling in SiMa Human Neuroblastoma Cells. Article Snippet: For Article Title: SPARC promotes pancreatic cancer cell proliferation and migration through autocrine secretion into the extracellular milieu. Article Snippet: The sequence of siRNA1 was used for the construction of SPARC shRNA using a pLKO.1‐puro plasmid (Sigma‐Aldrich; Merck KGaA). Article Title: ALDH1A1 confers resistance to RAF/MEK inhibitors in melanoma cells by maintaining stemness phenotype and activating PI3K/AKT signaling Article Snippet: Page 5/23 To achieve a stable ALDH1A1 knockdown (ALDH1A1KD), 1.5×105 melanoma cells were seeded on 6- multiplates and transduced at 70% Article Title: ALDH1A1 overexpression in melanoma cells promotes tumor angiogenesis by activating the IL-8/Notch signaling cascade Article Snippet: To achieve a stable knockdown, 1.5×10 5 melanoma cells were seeded on 6-multiplates and transduced at 70% confluence with Article Title: ALDH1A1 confers resistance to RAF/MEK inhibitors in melanoma cells by maintaining stemness phenotype and activating PI3K/AKT signaling. Article Snippet: When 70 % of confluence was reached, the Article Title: Interactions of Antibodies to the Gram-Negative Gastric Bacterium Helicobacter pylori with the Synaptic Calcium Sensor Synaptotagmin 5, Correlate to Impaired Vesicle Recycling in SiMa Human Neuroblastoma Cells Article Snippet: For Transfection:Article Title: All-trans retinoic acid-mediated ADAR1 degradation synergizes with PD-1 blockade to suppress pancreatic cancer Article Snippet: The pLKO.1-shLuc control plasmid (TRCN0000072243), pLKO.1-shLacZ control plasmid (TRCN0000072237), and ADAR short hairpin RNAs (TRCN0000301036 and TRCN0000050788), used for Article Title: Interactions of Antibodies to the Gram-Negative Gastric Bacterium Helicobacter pylori with the Synaptic Calcium Sensor Synaptotagmin 5, Correlate to Impaired Vesicle Recycling in SiMa Human Neuroblastoma Cells. Article Snippet: For Article Title: SPARC promotes pancreatic cancer cell proliferation and migration through autocrine secretion into the extracellular milieu. Article Snippet: The sequence of siRNA1 was used for the construction of SPARC shRNA using a pLKO.1‐puro plasmid (Sigma‐Aldrich; Merck KGaA). Article Title: ALDH1A1 confers resistance to RAF/MEK inhibitors in melanoma cells by maintaining stemness phenotype and activating PI3K/AKT signaling Article Snippet: Page 5/23 To achieve a stable ALDH1A1 knockdown (ALDH1A1KD), 1.5×105 melanoma cells were seeded on 6- multiplates and transduced at 70% Article Title: ALDH1A1 overexpression in melanoma cells promotes tumor angiogenesis by activating the IL-8/Notch signaling cascade Article Snippet: To achieve a stable knockdown, 1.5×10 5 melanoma cells were seeded on 6-multiplates and transduced at 70% confluence with Article Title: ALDH1A1 confers resistance to RAF/MEK inhibitors in melanoma cells by maintaining stemness phenotype and activating PI3K/AKT signaling. Article Snippet: When 70 % of confluence was reached, the Article Title: Interactions of Antibodies to the Gram-Negative Gastric Bacterium Helicobacter pylori with the Synaptic Calcium Sensor Synaptotagmin 5, Correlate to Impaired Vesicle Recycling in SiMa Human Neuroblastoma Cells Article Snippet: For shRNA:Article Title: All-trans retinoic acid-mediated ADAR1 degradation synergizes with PD-1 blockade to suppress pancreatic cancer Article Snippet: The pLKO.1-shLuc control plasmid (TRCN0000072243), pLKO.1-shLacZ control plasmid (TRCN0000072237), and ADAR short hairpin RNAs (TRCN0000301036 and TRCN0000050788), used for Article Title: Interactions of Antibodies to the Gram-Negative Gastric Bacterium Helicobacter pylori with the Synaptic Calcium Sensor Synaptotagmin 5, Correlate to Impaired Vesicle Recycling in SiMa Human Neuroblastoma Cells. Article Snippet: For Article Title: SPARC promotes pancreatic cancer cell proliferation and migration through autocrine secretion into the extracellular milieu. Article Snippet: The sequence of siRNA1 was used for the construction of SPARC shRNA using a pLKO.1‐puro plasmid (Sigma‐Aldrich; Merck KGaA). Article Title: ALDH1A1 confers resistance to RAF/MEK inhibitors in melanoma cells by maintaining stemness phenotype and activating PI3K/AKT signaling Article Snippet: Page 5/23 To achieve a stable ALDH1A1 knockdown (ALDH1A1KD), 1.5×105 melanoma cells were seeded on 6- multiplates and transduced at 70% Article Title: ALDH1A1 overexpression in melanoma cells promotes tumor angiogenesis by activating the IL-8/Notch signaling cascade Article Snippet: To achieve a stable knockdown, 1.5×10 5 melanoma cells were seeded on 6-multiplates and transduced at 70% confluence with Article Title: ALDH1A1 confers resistance to RAF/MEK inhibitors in melanoma cells by maintaining stemness phenotype and activating PI3K/AKT signaling. Article Snippet: When 70 % of confluence was reached, the Article Title: Interactions of Antibodies to the Gram-Negative Gastric Bacterium Helicobacter pylori with the Synaptic Calcium Sensor Synaptotagmin 5, Correlate to Impaired Vesicle Recycling in SiMa Human Neuroblastoma Cells Article Snippet: For Sequencing:Article Title: All-trans retinoic acid-mediated ADAR1 degradation synergizes with PD-1 blockade to suppress pancreatic cancer Article Snippet: The pLKO.1-shLuc control plasmid (TRCN0000072243), pLKO.1-shLacZ control plasmid (TRCN0000072237), and ADAR short hairpin RNAs (TRCN0000301036 and TRCN0000050788), used for Article Title: Interactions of Antibodies to the Gram-Negative Gastric Bacterium Helicobacter pylori with the Synaptic Calcium Sensor Synaptotagmin 5, Correlate to Impaired Vesicle Recycling in SiMa Human Neuroblastoma Cells. Article Snippet: For Article Title: SPARC promotes pancreatic cancer cell proliferation and migration through autocrine secretion into the extracellular milieu. Article Snippet: The sequence of siRNA1 was used for the construction of SPARC shRNA using a pLKO.1‐puro plasmid (Sigma‐Aldrich; Merck KGaA). Article Title: ALDH1A1 confers resistance to RAF/MEK inhibitors in melanoma cells by maintaining stemness phenotype and activating PI3K/AKT signaling Article Snippet: Page 5/23 To achieve a stable ALDH1A1 knockdown (ALDH1A1KD), 1.5×105 melanoma cells were seeded on 6- multiplates and transduced at 70% Article Title: ALDH1A1 overexpression in melanoma cells promotes tumor angiogenesis by activating the IL-8/Notch signaling cascade Article Snippet: To achieve a stable knockdown, 1.5×10 5 melanoma cells were seeded on 6-multiplates and transduced at 70% confluence with Article Title: ALDH1A1 confers resistance to RAF/MEK inhibitors in melanoma cells by maintaining stemness phenotype and activating PI3K/AKT signaling. Article Snippet: When 70 % of confluence was reached, the Article Title: Interactions of Antibodies to the Gram-Negative Gastric Bacterium Helicobacter pylori with the Synaptic Calcium Sensor Synaptotagmin 5, Correlate to Impaired Vesicle Recycling in SiMa Human Neuroblastoma Cells Article Snippet: For |

