test plasmids (Promega)
90
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Promega
test plasmids
Test Plasmids, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+vector+(pgl3-basic)/test+plasmid/pm25050743-83-7-18
Average 90 stars, based on 1 article reviews
Test Plasmids, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+vector+(pgl3-basic)/test+plasmid/pm25050743-83-7-18
Average 90 stars, based on 1 article reviews
test plasmids - by Bioz Stars,
2026-09
90/100 stars
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Plasmid Preparation:Article Title: Gene expression of the dibasic-pair cleaving enzyme NRD convertase (N-arginine dibasic convertase) is differentially regulated in the GH3 pituitary and Mat-Lu prostate cell lines Article Snippet: .. Each well contained 150 ng of Article Title: Aberrant over-expression of a forkhead family member, FOXO1A , in a brain tumor cell line Article Snippet: Wild-type and mutant genomic PCR products spanning 1161 bp of sequence from -866 to +296 relative to the FOXO1A transcription start site were generated from PER-453 and cloned into the luciferase vector, pGL3-basic (Promega). .. Human embryonic kidney (HEK) cells were transfected simultaneously with 400 ng of Article Title: The p38 Mitogen-activated Kinase Pathway Regulates the Human Interleukin-10 Promoter via the Activation of Sp1 Transcription Factor in Lipopolysaccharide-stimulated Human Macrophages Article Snippet: Transient transfection of cells and measurement of luciferase activity - Transfection of THP-1 and CD14-transfected THP-1 (THP-1/CD14) cells with plasmids containing various IL-10 promoter fragments was performed using LIPOFECTAMINE Reagent (Life Technologies, Inc. by guest on D ecem ber 28, 2016 http://w w w .jbc.org/ D ow nloaded from 10 Burlington, ON) following the manufacture’s instructions. .. 10 μg of the Article Title: AP-2α downregulation by cigarette smoke condensate is counteracted by p53 in human lung cancer cells. Article Snippet: The Dual-Luciferase® Reporter Assay System (Promega, Madison, WI, USA) was used to detect AP-2α promoter activity. .. Briefly, the cells were co-transfected with the Article Title: Multiple transcripts of sodium channel SCN8A (Na(V)1.6) with alternative 5'- and 3'-untranslated regions and initial characterization of the SCN8A promoter. Article Snippet: To identify the transcriptional start sites of the neuronal channel SCN8A, we carried out 5V -RACE (rapid amplification of cDNA ends) with RNA from human and mouse brain.. We recovered four mutually exclusive 5V -untranslated exons (exon 1a to exon 1d) that map to a 1.8-kb region of genomic DNA located ~70 kb upstream of the first coding exon.. The same 5V -untranslated exons are expressed in central, peripheral and sympathetic nervous system and in embryonic and adult brain. Article Title: Genetic analysis of the calcineurin pathway identifies members of the EGR gene family, specifically EGR3 , as potential susceptibility candidates in schizophrenia Article Snippet: The day before transfection, NB1 or IMR32 cells (the Japanese Collection of Research Bioresources Gene Bank, http://genebank.nibio.go.jp/gbank/index_e.html ) were plated at 1 × 10 5 cells per well in a 24-well plate and grown in DMEM (Sigma, St Louis, MO) supplemented with 10% FBS (Equitech-Bio, Kerrville, TX). .. One microgram of a Article Title: Functional structure of the promoter regions for the predominant low molecular weight isoforms of tropomyosin in human kidney cells. Article Snippet: High and low molecular weight (LMW) tropomyosin isoforms, by regulation of actin filaments, have a major role in the regulation of cell behaviour.. They affect malignant transformation, motility, differentiation, metastasis and cell membrane protein presentation.. Expression of LMW isoforms from the TPM1 and TPM3 genes have an important role in these effects but the regulation of their expression is unknown. Article Title: Transcriptional regulatory defects in the first intron ofBruton’s tyrosine kinase Article Snippet: Background : X-linked agammaglobulinemia (XLA), characterized by the early onset of recurrent bacterial infections, profound hypogammaglobulinemia, and a markedly diminished number of peripheral B lymphocytes, is caused by mutations in the Bruton’s tyrosine kinase ( BTK ) gene.. The >600 unique mutations identifi ed to date include single base pair substitutions, small insertions or deletions, and gross deletions.. A few cases, however, have been found to have no mutations in the coding region even with reduced BTK mRNA or protein expression. Transfection:Article Title: Aberrant over-expression of a forkhead family member, FOXO1A , in a brain tumor cell line Article Snippet: Wild-type and mutant genomic PCR products spanning 1161 bp of sequence from -866 to +296 relative to the FOXO1A transcription start site were generated from PER-453 and cloned into the luciferase vector, pGL3-basic (Promega). .. Human embryonic kidney (HEK) cells were transfected simultaneously with 400 ng of Article Title: Multiple transcripts of sodium channel SCN8A (Na(V)1.6) with alternative 5'- and 3'-untranslated regions and initial characterization of the SCN8A promoter. Article Snippet: To identify the transcriptional start sites of the neuronal channel SCN8A, we carried out 5V -RACE (rapid amplification of cDNA ends) with RNA from human and mouse brain.. We recovered four mutually exclusive 5V -untranslated exons (exon 1a to exon 1d) that map to a 1.8-kb region of genomic DNA located ~70 kb upstream of the first coding exon.. The same 5V -untranslated exons are expressed in central, peripheral and sympathetic nervous system and in embryonic and adult brain. Article Title: Transcriptional regulatory defects in the first intron ofBruton’s tyrosine kinase Article Snippet: Background : X-linked agammaglobulinemia (XLA), characterized by the early onset of recurrent bacterial infections, profound hypogammaglobulinemia, and a markedly diminished number of peripheral B lymphocytes, is caused by mutations in the Bruton’s tyrosine kinase ( BTK ) gene.. The >600 unique mutations identifi ed to date include single base pair substitutions, small insertions or deletions, and gross deletions.. A few cases, however, have been found to have no mutations in the coding region even with reduced BTK mRNA or protein expression. Expressing:Article Title: Aberrant over-expression of a forkhead family member, FOXO1A , in a brain tumor cell line Article Snippet: Wild-type and mutant genomic PCR products spanning 1161 bp of sequence from -866 to +296 relative to the FOXO1A transcription start site were generated from PER-453 and cloned into the luciferase vector, pGL3-basic (Promega). .. Human embryonic kidney (HEK) cells were transfected simultaneously with 400 ng of Control:Article Title: Aberrant over-expression of a forkhead family member, FOXO1A , in a brain tumor cell line Article Snippet: Wild-type and mutant genomic PCR products spanning 1161 bp of sequence from -866 to +296 relative to the FOXO1A transcription start site were generated from PER-453 and cloned into the luciferase vector, pGL3-basic (Promega). .. Human embryonic kidney (HEK) cells were transfected simultaneously with 400 ng of Article Title: The p38 Mitogen-activated Kinase Pathway Regulates the Human Interleukin-10 Promoter via the Activation of Sp1 Transcription Factor in Lipopolysaccharide-stimulated Human Macrophages Article Snippet: Transient transfection of cells and measurement of luciferase activity - Transfection of THP-1 and CD14-transfected THP-1 (THP-1/CD14) cells with plasmids containing various IL-10 promoter fragments was performed using LIPOFECTAMINE Reagent (Life Technologies, Inc. by guest on D ecem ber 28, 2016 http://w w w .jbc.org/ D ow nloaded from 10 Burlington, ON) following the manufacture’s instructions. .. 10 μg of the Article Title: Functional structure of the promoter regions for the predominant low molecular weight isoforms of tropomyosin in human kidney cells. Article Snippet: High and low molecular weight (LMW) tropomyosin isoforms, by regulation of actin filaments, have a major role in the regulation of cell behaviour.. They affect malignant transformation, motility, differentiation, metastasis and cell membrane protein presentation.. Expression of LMW isoforms from the TPM1 and TPM3 genes have an important role in these effects but the regulation of their expression is unknown. Article Title: Transcriptional regulatory defects in the first intron ofBruton’s tyrosine kinase Article Snippet: Background : X-linked agammaglobulinemia (XLA), characterized by the early onset of recurrent bacterial infections, profound hypogammaglobulinemia, and a markedly diminished number of peripheral B lymphocytes, is caused by mutations in the Bruton’s tyrosine kinase ( BTK ) gene.. The >600 unique mutations identifi ed to date include single base pair substitutions, small insertions or deletions, and gross deletions.. A few cases, however, have been found to have no mutations in the coding region even with reduced BTK mRNA or protein expression. Incubation:Article Title: The p38 Mitogen-activated Kinase Pathway Regulates the Human Interleukin-10 Promoter via the Activation of Sp1 Transcription Factor in Lipopolysaccharide-stimulated Human Macrophages Article Snippet: Transient transfection of cells and measurement of luciferase activity - Transfection of THP-1 and CD14-transfected THP-1 (THP-1/CD14) cells with plasmids containing various IL-10 promoter fragments was performed using LIPOFECTAMINE Reagent (Life Technologies, Inc. by guest on D ecem ber 28, 2016 http://w w w .jbc.org/ D ow nloaded from 10 Burlington, ON) following the manufacture’s instructions. .. 10 μg of the Luciferase:Article Title: Multiple transcripts of sodium channel SCN8A (Na(V)1.6) with alternative 5'- and 3'-untranslated regions and initial characterization of the SCN8A promoter. Article Snippet: To identify the transcriptional start sites of the neuronal channel SCN8A, we carried out 5V -RACE (rapid amplification of cDNA ends) with RNA from human and mouse brain.. We recovered four mutually exclusive 5V -untranslated exons (exon 1a to exon 1d) that map to a 1.8-kb region of genomic DNA located ~70 kb upstream of the first coding exon.. The same 5V -untranslated exons are expressed in central, peripheral and sympathetic nervous system and in embryonic and adult brain. |
