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pgl3 basic plasmids  (Addgene inc)


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    Structured Review

    Addgene inc pgl3 basic plasmids
    Pgl3 Basic Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+pgl3+basic+plasmid/pGL3+(-BRE)+Luciferase+(Plasmid+%2345127)/pm38429817-196-9-11
    Average 95 stars, based on 195 article reviews
    pgl3 basic plasmids - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Method for preparing
    Article Snippet: .. COS7 monkey kidney cells (ATCC) were cultured on a 24-well plate at 1.5×105 cells/well and then a pGL3-PGC-1α-Luc plasm id (Addgene, Cambridge, MA, USA) was transfected into the cells using Lipofector (Aptabio, Yongin, Korea). ..

    Transfection:

    Article Title: Method for preparing
    Article Snippet: .. COS7 monkey kidney cells (ATCC) were cultured on a 24-well plate at 1.5×105 cells/well and then a pGL3-PGC-1α-Luc plasm id (Addgene, Cambridge, MA, USA) was transfected into the cells using Lipofector (Aptabio, Yongin, Korea). ..

    Plasmid Preparation:

    Article Title: The natural L370F ERα variant confers endocrine resistance and sensitivity to ATRA in metastatic breast cancer cells
    Article Snippet: .. To generate the plasmid pGL2Basic_Neo_RARE-NLuc-PEST, the RARE cassette was KpnI and HindIII excised from the pGL3-RARE-luciferase [ ] purchased from Addgene (USA) and ligated into the corresponding restriction sites in the pGL2Basic_Neo_NLuc-PEST [ , ]. ..

    Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1
    Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the pGL3-basic luciferase reporter plasmid (Addgene). ..

    Article Title: The natural L370F ERα variant confers endocrine resistance and sensitivity to ATRA in metastatic breast cancer cells.
    Article Snippet: .. To generate the plasmid pGL2Basic_Neo_RARE-NLuc-PEST, the RARE cassette was KpnI and HindIII excised from the pGL3-RAREluciferase [25] purchased from Addgene (USA) and ligated into the corresponding restriction sites in the pGL2Basic_Neo_NLuc-PEST [22, 23]. ..

    Article Title: Evidence for G-quadruplex-mediated transactivation by the immediate-early 2 protein of human cytomegalovirus
    Article Snippet: .. To produce the UL146 promoter-driven luciferase reporter plasmid (UL146p-Luc), the UL146 promoter region (−927 to +108) was PCR-amplified as an NheI/NcoI fragment and cloned into pGL3-basic (Addgene). ..

    Article Title: METTL3 Methylation Induces Decay of Endogenous Retroelement Transcripts to Promote Tumor Immune Evasion
    Article Snippet: The genes of SMYD1-5, 177 SETD7, SETD8 were inserted into pcDNA3.1(+) vector (RRID:Addgene_52535). .. The 178 SETD1A and E2F4 promoter region, spanning nucleotides −2000 to +100 relative to 179 the transcription start site (TSS), was PCR-amplified and ligated into the pGL3-Basic 180 luciferase reporter plasmid (RRID:Addgene_212936). ..

    Article Title: Ebselen Suppresses Breast Cancer Tumorigenesis by Inhibiting YTHDF1-Mediated c-Fos Expression.
    Article Snippet: To design the translation reporter construct, the putative m6A modification site of FOS (chr4:5278828–75282230) was PCR-amplified from MCF7 cDNA and cloned into the 3′-UTR of RLuc at the XhoI site of the psiCHECK3 vector (a gift from Anthony Leung; Addgene plasmid #136010). .. The pGL3-FOS-Luc plasmid was a gift from Ron Prywes (Addgene plasmid #11983). ..

    Amplification:

    Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1
    Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the pGL3-basic luciferase reporter plasmid (Addgene). ..

    Polymerase Chain Reaction:

    Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1
    Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the pGL3-basic luciferase reporter plasmid (Addgene). ..

    Article Title: Evidence for G-quadruplex-mediated transactivation by the immediate-early 2 protein of human cytomegalovirus
    Article Snippet: .. To produce the UL146 promoter-driven luciferase reporter plasmid (UL146p-Luc), the UL146 promoter region (−927 to +108) was PCR-amplified as an NheI/NcoI fragment and cloned into pGL3-basic (Addgene). ..

    Article Title: METTL3 Methylation Induces Decay of Endogenous Retroelement Transcripts to Promote Tumor Immune Evasion
    Article Snippet: The genes of SMYD1-5, 177 SETD7, SETD8 were inserted into pcDNA3.1(+) vector (RRID:Addgene_52535). .. The 178 SETD1A and E2F4 promoter region, spanning nucleotides −2000 to +100 relative to 179 the transcription start site (TSS), was PCR-amplified and ligated into the pGL3-Basic 180 luciferase reporter plasmid (RRID:Addgene_212936). ..

    Clone Assay:

    Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1
    Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the pGL3-basic luciferase reporter plasmid (Addgene). ..

    Article Title: Evidence for G-quadruplex-mediated transactivation by the immediate-early 2 protein of human cytomegalovirus
    Article Snippet: .. To produce the UL146 promoter-driven luciferase reporter plasmid (UL146p-Luc), the UL146 promoter region (−927 to +108) was PCR-amplified as an NheI/NcoI fragment and cloned into pGL3-basic (Addgene). ..

    Luciferase:

    Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1
    Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the pGL3-basic luciferase reporter plasmid (Addgene). ..

    Article Title: tRF-21 LeuTAA Promotes Oxidative Stress by Altering Glutathione Metabolic Enzymes to Support Prostate Cancer Progression
    Article Snippet: Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, and Reactome analyses and visualization were performed using OmicShare ( https://www.omicshare.com ) and SangerBox ( http://www.sangerbox.com/ ). .. The firefly luciferase–expressing plasmids pGL3-basic (RRID:Addgene_212936), pGL3-promoter (RRID:Addgene_172006), and Renilla pRL-CMV (RRID:Addgene_41046) were purchased from Promega (TM033, E2261). ..

    Article Title: Evidence for G-quadruplex-mediated transactivation by the immediate-early 2 protein of human cytomegalovirus
    Article Snippet: .. To produce the UL146 promoter-driven luciferase reporter plasmid (UL146p-Luc), the UL146 promoter region (−927 to +108) was PCR-amplified as an NheI/NcoI fragment and cloned into pGL3-basic (Addgene). ..

    Article Title: METTL3 Methylation Induces Decay of Endogenous Retroelement Transcripts to Promote Tumor Immune Evasion
    Article Snippet: The genes of SMYD1-5, 177 SETD7, SETD8 were inserted into pcDNA3.1(+) vector (RRID:Addgene_52535). .. The 178 SETD1A and E2F4 promoter region, spanning nucleotides −2000 to +100 relative to 179 the transcription start site (TSS), was PCR-amplified and ligated into the pGL3-Basic 180 luciferase reporter plasmid (RRID:Addgene_212936). ..



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    Results from gene reporter assays and transcriptional activity of the characterized promotor haplotypes.

    Journal: Genes

    Article Title: Functional Analysis of Promoter Variants in Genes Involved in Sex Steroid Action, DNA Repair and Cell Cycle Control

    doi: 10.3390/genes10030186

    Figure Lengend Snippet: Results from gene reporter assays and transcriptional activity of the characterized promotor haplotypes.

    Article Snippet: Similar experiments were performed with the empty promoterless pGL3-Basic plasmid (Promega) and the SV40-driven Firefly Luciferase pGL3-control plasmid (Promega, Madison, WI, USA) as negative and positive controls, respectively.

    Techniques: Activity Assay, Luciferase, Plasmid Preparation, Clone Assay, Control, Expressing