sv40 prey fusion (Becton Dickinson)
Structured Review

Sv40 Prey Fusion, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/empty+pgadt7+vector/sv40/pmc03183051-48-19-22
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "Co-Interactive DNA-Binding between a Novel, Immunophilin-Like Shrimp Protein and VP15 Nucleocapsid Protein of White Spot Syndrome Virus"
Article Title: Co-Interactive DNA-Binding between a Novel, Immunophilin-Like Shrimp Protein and VP15 Nucleocapsid Protein of White Spot Syndrome Virus
Journal: PLoS ONE
doi: 10.1371/journal.pone.0025420
Figure Legend Snippet: ( A ) Photographic film of immunoblot detection of BD-VP15 fusion protein with anti c-Myc antibody, confirming its expression in transformed Y187 compared to un-transformed Y187 (-ve). ( B ) Yeast two-hybrid screen results indicating the presence of BD and AD-plasmids in transformant cells growing on an SD/-Leu/-Trp plate. Putative interaction of BD-VP15 and AD-PmFKBP46c is indicated by growth and blue color on selective medium. The positive control comprised interaction between murine p53 bait fusion and SV40 prey fusion (BD Biosciences). The negative controls comprised yeast cells containing BD-VP15 and empty pGADT7 vector or empty pGBKT7 vector and AD-PmFKBP46c.
Techniques Used: Western Blot, Expressing, Transformation Assay, Two Hybrid Screening, Positive Control, Plasmid Preparation
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