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Structured Review

Becton Dickinson sv40 prey fusion
( A ) Photographic film of immunoblot detection of BD-VP15 fusion protein with anti c-Myc antibody, confirming its expression in transformed Y187 compared to un-transformed Y187 (-ve). ( B ) Yeast two-hybrid screen results indicating the presence of BD and AD-plasmids in transformant cells growing on an SD/-Leu/-Trp plate. Putative interaction of BD-VP15 and AD-PmFKBP46c is indicated by growth and blue color on selective medium. The positive control comprised interaction between murine p53 bait fusion and <t>SV40</t> prey fusion (BD Biosciences). The negative controls comprised yeast cells containing BD-VP15 and empty pGADT7 vector or empty pGBKT7 vector and AD-PmFKBP46c.
Sv40 Prey Fusion, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Co-Interactive DNA-Binding between a Novel, Immunophilin-Like Shrimp Protein and VP15 Nucleocapsid Protein of White Spot Syndrome Virus"

Article Title: Co-Interactive DNA-Binding between a Novel, Immunophilin-Like Shrimp Protein and VP15 Nucleocapsid Protein of White Spot Syndrome Virus

Journal: PLoS ONE

doi: 10.1371/journal.pone.0025420

( A ) Photographic film of immunoblot detection of BD-VP15 fusion protein with anti c-Myc antibody, confirming its expression in transformed Y187 compared to un-transformed Y187 (-ve). ( B ) Yeast two-hybrid screen results indicating the presence of BD and AD-plasmids in transformant cells growing on an SD/-Leu/-Trp plate. Putative interaction of BD-VP15 and AD-PmFKBP46c is indicated by growth and blue color on selective medium. The positive control comprised interaction between murine p53 bait fusion and SV40 prey fusion (BD Biosciences). The negative controls comprised yeast cells containing BD-VP15 and empty pGADT7 vector or empty pGBKT7 vector and AD-PmFKBP46c.
Figure Legend Snippet: ( A ) Photographic film of immunoblot detection of BD-VP15 fusion protein with anti c-Myc antibody, confirming its expression in transformed Y187 compared to un-transformed Y187 (-ve). ( B ) Yeast two-hybrid screen results indicating the presence of BD and AD-plasmids in transformant cells growing on an SD/-Leu/-Trp plate. Putative interaction of BD-VP15 and AD-PmFKBP46c is indicated by growth and blue color on selective medium. The positive control comprised interaction between murine p53 bait fusion and SV40 prey fusion (BD Biosciences). The negative controls comprised yeast cells containing BD-VP15 and empty pGADT7 vector or empty pGBKT7 vector and AD-PmFKBP46c.

Techniques Used: Western Blot, Expressing, Transformation Assay, Two Hybrid Screening, Positive Control, Plasmid Preparation

Related Articles

other:

Article Title: Revisiting G3BP1 as a RasGAP Binding Protein: Sensitization of Tumor Cells to Chemotherapy by the RasGAP 317–326 Sequence Does Not Involve G3BP1
Article Snippet: The monoclonal mouse anti-SV40 large T antigen was from BD Pharmingen (reference n°554149).

Article Title: Immunoregulatory Sertoli Cell Allografts Engineered to Express Human Insulin Survive Humoral-Mediated Rejection.
Article Snippet: For cell survival, tissues were incubated with monoclonal mouse anti-SV40 large T antigen antibody (1:100 dilution: BD Biosciences, San Jose, CA, USA).

Article Title: Immunoregulatory Sertoli Cell Allografts Engineered to Express Human Insulin Survive Humoral-Mediated Rejection
Article Snippet: For cell survival, tissues were incubated with monoclonal mouse anti-SV40 large T antigen antibody (1:100 dilution: BD Biosciences, San Jose, CA, USA).

Article Title: The Role of SRC-1 in Murine Prostate Carcinogenesis Is Nonessential due to a Possible Compensation of SRC-3/AIB1 Overexpression
Article Snippet: Sections were subsequently incubated overnight at 4 o C with following primary antibodies: Mouse monoclonal anti-T antigen (BD Transduction Laboratory) (for detection of SV40 large T-antigen), goat polyclonal anti-SRC-1 (Santa Cruz) and rabbit polyclonal anti-SRC-3 (Cell Signaling) antibodies.

Positive Control:

Article Title: Co-Interactive DNA-Binding between a Novel, Immunophilin-Like Shrimp Protein and VP15 Nucleocapsid Protein of White Spot Syndrome Virus
Article Snippet: .. The empty vectors pGADT7 or pGBKT7 were used as negative controls and interaction between murine p53 bait fusion and SV40 prey fusion (BD Biosciences) served as a positive control. ..



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Image Search Results


Analysis of the interaction between LrWRKY3 and LrDef1 promoter. a EMSA revealed that the LrWRKY3 specifically bound to W box. 1, reaction solution containing only free probes; 2, LrWRKY3 can interact with biotin labelled probes containing the W box sequence; 3, LrWRKY3 can both interact with biotin labelled probes containing the W box sequence and unlabeled probes containing the W box sequence; 4, LrWRKY3 can’t interact with mutant probes; Complex: the combination of LrWRKY3 recombinant protein and probes; Free probe: the unbound probes. b Analysis of the transactivation effect of LrWRKY3 on LrDef1 promoter. LrWRKY3 + pLrDef1 : transcriptional activity analysis of pGADT7- LrWRKY3 interaction with pLrDef1 ; Positive control: pGADT7- p53 can interact with pAbAi- p53 as a positive control; Negative control: pAbAi empty vector was used a negative control. c GUS activity was higher in transgenic tobacco lines co-expressing of LrWRKY3 and LrDef1 promoter than in the transgenic tobacco lines expressing of LrDef1 promoter. The results were shown as average values calculated from three replicates and calculated by the 2 -ΔΔCt method and analyzed by the Student’s t test. pBI121: pBI121- GUS transgenic tobacco; PD-2/3/7/10: pLrDef1 transgenic tobacco lines. Co-1/7/9/12: LrWRKY3 / pLrDef1 transgenic tobacco lines

Journal: BMC Plant Biology

Article Title: Lilium regale Wilson WRKY3 modulates an antimicrobial peptide gene, LrDef1 , during response to Fusarium oxysporum

doi: 10.1186/s12870-022-03649-y

Figure Lengend Snippet: Analysis of the interaction between LrWRKY3 and LrDef1 promoter. a EMSA revealed that the LrWRKY3 specifically bound to W box. 1, reaction solution containing only free probes; 2, LrWRKY3 can interact with biotin labelled probes containing the W box sequence; 3, LrWRKY3 can both interact with biotin labelled probes containing the W box sequence and unlabeled probes containing the W box sequence; 4, LrWRKY3 can’t interact with mutant probes; Complex: the combination of LrWRKY3 recombinant protein and probes; Free probe: the unbound probes. b Analysis of the transactivation effect of LrWRKY3 on LrDef1 promoter. LrWRKY3 + pLrDef1 : transcriptional activity analysis of pGADT7- LrWRKY3 interaction with pLrDef1 ; Positive control: pGADT7- p53 can interact with pAbAi- p53 as a positive control; Negative control: pAbAi empty vector was used a negative control. c GUS activity was higher in transgenic tobacco lines co-expressing of LrWRKY3 and LrDef1 promoter than in the transgenic tobacco lines expressing of LrDef1 promoter. The results were shown as average values calculated from three replicates and calculated by the 2 -ΔΔCt method and analyzed by the Student’s t test. pBI121: pBI121- GUS transgenic tobacco; PD-2/3/7/10: pLrDef1 transgenic tobacco lines. Co-1/7/9/12: LrWRKY3 / pLrDef1 transgenic tobacco lines

Article Snippet: Moreover, the yeast prey empty vector pGADT7 AD was transferred into bait strain pAbAi- pLrDef1- Y1HGold competent cells as a negative control, and the pGADT7 AD- P53 vector (kit provided, Takara) was transferred into bait vector pAbAi- p53 -Y1HGOLD competent cells as a positive control.

Techniques: Sequencing, Mutagenesis, Recombinant, Activity Assay, Positive Control, Negative Control, Plasmid Preparation, Transgenic Assay, Expressing