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drosophila s2 cell line  (ATCC)


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    Structured Review

    ATCC drosophila s2 cell line

    Drosophila S2 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/embryo/D%2E+Mel%2E+(2)%3B+Embryo%3B+Fruit+Fly/pmc08428331-88-0-5
    Average 95 stars, based on 89 article reviews
    drosophila s2 cell line - by Bioz Stars, 2026-11
    95/100 stars

    Images

    1) Product Images from "BAP1 enhances Polycomb repression by counteracting widespread H2AK119ub1 deposition and chromatin condensation"

    Article Title: BAP1 enhances Polycomb repression by counteracting widespread H2AK119ub1 deposition and chromatin condensation

    Journal: Molecular Cell

    doi: 10.1016/j.molcel.2021.06.020


    Figure Legend Snippet:

    Techniques Used: Control, Virus, Recombinant, Transfection, Purification, Extraction, Sequencing, Imaging, Plasmid Preparation, Software

    Related Articles

    Mouse Assay:

    Article Title: Mapping catalytically engaged TOP2B in neurons reveals the principles of topoisomerase action within the genome.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-TOP2B Santa Cruz RRID:AB_628384 Rabbit monoclonal anti-CTCF Cell Signaling RRID:AB_2086791 Chemicals, peptides, and recombinant proteins Etoposide Sigma Cat#:E1383 ICRF-193 Sigma Cat#I4659 Pierce ChIP-grade Protein A/G magnetic beads ThermoFisher Cat#:26162 Lambda Exonuclease NEB Cat#:M0262S Phusion DNA Polymerase NEB Cat#:M0530S AMPure XP Beads Beckman Cat#:A63881 rGTP VWR Cat#:PAE6031 TdT NEB Cat#:M0315S Proteinase K NEB Cat#:P8107S Superase-IN ThermoFisher Cat#:AM2694 Trizol LS ThermoFisher Cat#:10296010 4-thio-UTP FisherScientific Cat#:NC0091659 4-thiouridine Sigma Cat#:T4509 EZ-link HPDP-biotin ThermoFisher Cat#:A35390 M280 Streptavidin Dynabeads ThermoFisher Cat#:11205D Critical commercial assays KAPA HyperPrep Kit Roche Cat#: 07962363001 Qiagen RNeasy Plus Universal Kit Qiagen Cat#:74034 TruSeq stranded mRNA library prep Illumina Cat#:20020594 Zymo Clean and Concentrator Kit Zymo Cat#R1013 KAPA RNA HyperPrep Kit KAPA Cat#:08098093702 Deposited data H3K9me3 – P0 Forebrain ENCODE ENCSR093DWU H3K27me3 – P0 Forebrain ENCODE ENCSR070MOK H3K36me3– P0 Forebrain ENCODE ENCSR069TDC H3K4me2– P0 Forebrain ENCODE ENCSR906UJW H3K4me3– P0 Forebrain ENCODE ENCSR258YWW H3K4me1– P0 Forebrain ENCODE ENCSR465PLB H3K9ac– P0 Forebrain ENCODE ENCSR369RBO H3K27ac– P0 Forebrain ENCODE ENCSR094TTT POL2A – E14.5 Forebrain ENCODE ENCSR000CEI HiC – Cortical Neurons Bonev et al.48 GEO: GSE96107 END-seq Canela et al.26 GEO: GSE99194 TOP2B-ChIP seq Madabhushi et al.24 GEO: GSE61887 gH2AX ChIP-seq Madabhushi et al.24 GEO: GSE61887 TOP2Bcc-seq This Paper GEO: GSE227463 fastGro seq – Control Neurons This Paper GEO: GSE227463 fastGro seq – ETP treated Neurons This Paper GEO: GSE227463 fastGro seq – ICRF-193 treated Neurons This Paper GEO: GSE227463 RNA-seq Control Neurons This Paper GEO: GSE227463 (Continued on next page) 18 Cell Reports 43, 113809, February 27, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines Schneider’s Drosophila Line 2 (S2) ATCC CRL-1963 Experimental models: Organisms/strains Swiss Webster Mice Charles River Strain 024 Oligonucleotides P7 extension primer: CAAGCAGAAGACGGCATACGA*G (* denotes phophorothioate linkage) This paper, supplied by IDT N/A CCC overhang adapter oligo1: AATGATACGGCGACCACCGAGATCTACA CTCTTTCCCTACACGACGCTCTTCCGATC Teves et al.,35 supplied by IDT N/A CCC overhang adapter oligo2: (Phosphate)GA TCGGAAGAGCGGTTCAGCAGGAATGCCGAG Teves et al.,35 supplied by IDT N/A Software and algorithms FastQC S. Andrew72 https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ Trimmomatic Bolger et al.73 http://www.usadellab.org/cms/ index.php?page=trimmomatic BWA Li et al.74 https://github.com/lh3/bwa Sambamba Tarasov et al.75 https://github.com/biod/sambamba/releases Samtools Danecek et al.76 http://www.htslib.org/ MACS2 Zhang et al.36 https://github.com/macs3-project/MACS UCSC Kent tools Kuhn et al.77 http://hgdownload.soe.ucsc.edu/downloads. html#source_downloads TopHat Kim et al.78 https://ccb.jhu.edu/software/tophat/index.shtml Cutadapt M. Martin79 https://github.com/marcelm/cutadapt/ Deeptools Ramirez et al.80 https://github.com/deeptools/deepTools HOMER Heinz et al.81 Wang et al.82 http://homer.ucsd.edu/homer/download.html DESeq2 Love et al.56 https://bioconductor.org/packages/release/ bioc/html/DESeq2.html dREG Wang et al.83 https://dreg.dnasequence.org/ RunOnBamToBigWig Chu et al.84 https://github.com/Danko-Lab/RunOnBamToBigWig Juicer Durand et al.85 https://github.com/aidenlab/juicer HiCCUPS Rao et al.47 https://github.com/aidenlab/juicer/wiki/HiCCUPS Arrowhead Rao et al.47 https://github.com/aidenlab/juicer/wiki/Arrowhead MEME-ChIP Machanick et al.86 https://meme-suite.org/meme/doc/meme-chip.html ChromHMM Ernst et al.40 http://compbio.mit.edu/ChromHMM/#: :text= ChromHMM%20is%20based%20on%20a, one%20or%20more%20cell%20types. ..

    Countercurrent Chromatography:

    Article Title: Mapping catalytically engaged TOP2B in neurons reveals the principles of topoisomerase action within the genome.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-TOP2B Santa Cruz RRID:AB_628384 Rabbit monoclonal anti-CTCF Cell Signaling RRID:AB_2086791 Chemicals, peptides, and recombinant proteins Etoposide Sigma Cat#:E1383 ICRF-193 Sigma Cat#I4659 Pierce ChIP-grade Protein A/G magnetic beads ThermoFisher Cat#:26162 Lambda Exonuclease NEB Cat#:M0262S Phusion DNA Polymerase NEB Cat#:M0530S AMPure XP Beads Beckman Cat#:A63881 rGTP VWR Cat#:PAE6031 TdT NEB Cat#:M0315S Proteinase K NEB Cat#:P8107S Superase-IN ThermoFisher Cat#:AM2694 Trizol LS ThermoFisher Cat#:10296010 4-thio-UTP FisherScientific Cat#:NC0091659 4-thiouridine Sigma Cat#:T4509 EZ-link HPDP-biotin ThermoFisher Cat#:A35390 M280 Streptavidin Dynabeads ThermoFisher Cat#:11205D Critical commercial assays KAPA HyperPrep Kit Roche Cat#: 07962363001 Qiagen RNeasy Plus Universal Kit Qiagen Cat#:74034 TruSeq stranded mRNA library prep Illumina Cat#:20020594 Zymo Clean and Concentrator Kit Zymo Cat#R1013 KAPA RNA HyperPrep Kit KAPA Cat#:08098093702 Deposited data H3K9me3 – P0 Forebrain ENCODE ENCSR093DWU H3K27me3 – P0 Forebrain ENCODE ENCSR070MOK H3K36me3– P0 Forebrain ENCODE ENCSR069TDC H3K4me2– P0 Forebrain ENCODE ENCSR906UJW H3K4me3– P0 Forebrain ENCODE ENCSR258YWW H3K4me1– P0 Forebrain ENCODE ENCSR465PLB H3K9ac– P0 Forebrain ENCODE ENCSR369RBO H3K27ac– P0 Forebrain ENCODE ENCSR094TTT POL2A – E14.5 Forebrain ENCODE ENCSR000CEI HiC – Cortical Neurons Bonev et al.48 GEO: GSE96107 END-seq Canela et al.26 GEO: GSE99194 TOP2B-ChIP seq Madabhushi et al.24 GEO: GSE61887 gH2AX ChIP-seq Madabhushi et al.24 GEO: GSE61887 TOP2Bcc-seq This Paper GEO: GSE227463 fastGro seq – Control Neurons This Paper GEO: GSE227463 fastGro seq – ETP treated Neurons This Paper GEO: GSE227463 fastGro seq – ICRF-193 treated Neurons This Paper GEO: GSE227463 RNA-seq Control Neurons This Paper GEO: GSE227463 (Continued on next page) 18 Cell Reports 43, 113809, February 27, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines Schneider’s Drosophila Line 2 (S2) ATCC CRL-1963 Experimental models: Organisms/strains Swiss Webster Mice Charles River Strain 024 Oligonucleotides P7 extension primer: CAAGCAGAAGACGGCATACGA*G (* denotes phophorothioate linkage) This paper, supplied by IDT N/A CCC overhang adapter oligo1: AATGATACGGCGACCACCGAGATCTACA CTCTTTCCCTACACGACGCTCTTCCGATC Teves et al.,35 supplied by IDT N/A CCC overhang adapter oligo2: (Phosphate)GA TCGGAAGAGCGGTTCAGCAGGAATGCCGAG Teves et al.,35 supplied by IDT N/A Software and algorithms FastQC S. Andrew72 https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ Trimmomatic Bolger et al.73 http://www.usadellab.org/cms/ index.php?page=trimmomatic BWA Li et al.74 https://github.com/lh3/bwa Sambamba Tarasov et al.75 https://github.com/biod/sambamba/releases Samtools Danecek et al.76 http://www.htslib.org/ MACS2 Zhang et al.36 https://github.com/macs3-project/MACS UCSC Kent tools Kuhn et al.77 http://hgdownload.soe.ucsc.edu/downloads. html#source_downloads TopHat Kim et al.78 https://ccb.jhu.edu/software/tophat/index.shtml Cutadapt M. Martin79 https://github.com/marcelm/cutadapt/ Deeptools Ramirez et al.80 https://github.com/deeptools/deepTools HOMER Heinz et al.81 Wang et al.82 http://homer.ucsd.edu/homer/download.html DESeq2 Love et al.56 https://bioconductor.org/packages/release/ bioc/html/DESeq2.html dREG Wang et al.83 https://dreg.dnasequence.org/ RunOnBamToBigWig Chu et al.84 https://github.com/Danko-Lab/RunOnBamToBigWig Juicer Durand et al.85 https://github.com/aidenlab/juicer HiCCUPS Rao et al.47 https://github.com/aidenlab/juicer/wiki/HiCCUPS Arrowhead Rao et al.47 https://github.com/aidenlab/juicer/wiki/Arrowhead MEME-ChIP Machanick et al.86 https://meme-suite.org/meme/doc/meme-chip.html ChromHMM Ernst et al.40 http://compbio.mit.edu/ChromHMM/#: :text= ChromHMM%20is%20based%20on%20a, one%20or%20more%20cell%20types. ..

    Software:

    Article Title: Mapping catalytically engaged TOP2B in neurons reveals the principles of topoisomerase action within the genome.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-TOP2B Santa Cruz RRID:AB_628384 Rabbit monoclonal anti-CTCF Cell Signaling RRID:AB_2086791 Chemicals, peptides, and recombinant proteins Etoposide Sigma Cat#:E1383 ICRF-193 Sigma Cat#I4659 Pierce ChIP-grade Protein A/G magnetic beads ThermoFisher Cat#:26162 Lambda Exonuclease NEB Cat#:M0262S Phusion DNA Polymerase NEB Cat#:M0530S AMPure XP Beads Beckman Cat#:A63881 rGTP VWR Cat#:PAE6031 TdT NEB Cat#:M0315S Proteinase K NEB Cat#:P8107S Superase-IN ThermoFisher Cat#:AM2694 Trizol LS ThermoFisher Cat#:10296010 4-thio-UTP FisherScientific Cat#:NC0091659 4-thiouridine Sigma Cat#:T4509 EZ-link HPDP-biotin ThermoFisher Cat#:A35390 M280 Streptavidin Dynabeads ThermoFisher Cat#:11205D Critical commercial assays KAPA HyperPrep Kit Roche Cat#: 07962363001 Qiagen RNeasy Plus Universal Kit Qiagen Cat#:74034 TruSeq stranded mRNA library prep Illumina Cat#:20020594 Zymo Clean and Concentrator Kit Zymo Cat#R1013 KAPA RNA HyperPrep Kit KAPA Cat#:08098093702 Deposited data H3K9me3 – P0 Forebrain ENCODE ENCSR093DWU H3K27me3 – P0 Forebrain ENCODE ENCSR070MOK H3K36me3– P0 Forebrain ENCODE ENCSR069TDC H3K4me2– P0 Forebrain ENCODE ENCSR906UJW H3K4me3– P0 Forebrain ENCODE ENCSR258YWW H3K4me1– P0 Forebrain ENCODE ENCSR465PLB H3K9ac– P0 Forebrain ENCODE ENCSR369RBO H3K27ac– P0 Forebrain ENCODE ENCSR094TTT POL2A – E14.5 Forebrain ENCODE ENCSR000CEI HiC – Cortical Neurons Bonev et al.48 GEO: GSE96107 END-seq Canela et al.26 GEO: GSE99194 TOP2B-ChIP seq Madabhushi et al.24 GEO: GSE61887 gH2AX ChIP-seq Madabhushi et al.24 GEO: GSE61887 TOP2Bcc-seq This Paper GEO: GSE227463 fastGro seq – Control Neurons This Paper GEO: GSE227463 fastGro seq – ETP treated Neurons This Paper GEO: GSE227463 fastGro seq – ICRF-193 treated Neurons This Paper GEO: GSE227463 RNA-seq Control Neurons This Paper GEO: GSE227463 (Continued on next page) 18 Cell Reports 43, 113809, February 27, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental models: Cell lines Schneider’s Drosophila Line 2 (S2) ATCC CRL-1963 Experimental models: Organisms/strains Swiss Webster Mice Charles River Strain 024 Oligonucleotides P7 extension primer: CAAGCAGAAGACGGCATACGA*G (* denotes phophorothioate linkage) This paper, supplied by IDT N/A CCC overhang adapter oligo1: AATGATACGGCGACCACCGAGATCTACA CTCTTTCCCTACACGACGCTCTTCCGATC Teves et al.,35 supplied by IDT N/A CCC overhang adapter oligo2: (Phosphate)GA TCGGAAGAGCGGTTCAGCAGGAATGCCGAG Teves et al.,35 supplied by IDT N/A Software and algorithms FastQC S. Andrew72 https://www.bioinformatics.babraham. ac.uk/projects/fastqc/ Trimmomatic Bolger et al.73 http://www.usadellab.org/cms/ index.php?page=trimmomatic BWA Li et al.74 https://github.com/lh3/bwa Sambamba Tarasov et al.75 https://github.com/biod/sambamba/releases Samtools Danecek et al.76 http://www.htslib.org/ MACS2 Zhang et al.36 https://github.com/macs3-project/MACS UCSC Kent tools Kuhn et al.77 http://hgdownload.soe.ucsc.edu/downloads. html#source_downloads TopHat Kim et al.78 https://ccb.jhu.edu/software/tophat/index.shtml Cutadapt M. Martin79 https://github.com/marcelm/cutadapt/ Deeptools Ramirez et al.80 https://github.com/deeptools/deepTools HOMER Heinz et al.81 Wang et al.82 http://homer.ucsd.edu/homer/download.html DESeq2 Love et al.56 https://bioconductor.org/packages/release/ bioc/html/DESeq2.html dREG Wang et al.83 https://dreg.dnasequence.org/ RunOnBamToBigWig Chu et al.84 https://github.com/Danko-Lab/RunOnBamToBigWig Juicer Durand et al.85 https://github.com/aidenlab/juicer HiCCUPS Rao et al.47 https://github.com/aidenlab/juicer/wiki/HiCCUPS Arrowhead Rao et al.47 https://github.com/aidenlab/juicer/wiki/Arrowhead MEME-ChIP Machanick et al.86 https://meme-suite.org/meme/doc/meme-chip.html ChromHMM Ernst et al.40 http://compbio.mit.edu/ChromHMM/#: :text= ChromHMM%20is%20based%20on%20a, one%20or%20more%20cell%20types. ..


    other:

    Article Title: Ehbp1 orchestrates orderly sorting of Wnt/Wingless to the basolateral and apical cell membranes
    Article Snippet: Schneider 2 ( D. melanogaster ) , ATCC , CRL-1963, CVCL_Z232.

    Recombinant:


    Isolation:


    Sequencing:


    Activation Assay:


    Bioassay:


    Derivative Assay:

    Article Title: Linker histone H1 regulates homeostasis of heterochromatin-associated cRNAs.
    Article Snippet: .. Drosophila melanogaster Schneider S2 cell line (ATCC-CRL-1963) used in these studies is a stable male cell line derived from late Oregon R embryos and was maintained in culture at 25 C in Schneider’s Insect Medium (L0207-500, Biowest) supplemented with 10% heat-inactivated FBS (10270, Gibco) and 1% penicillin-streptomycin (15140-122, Gibco). ..

    Cell Culture:

    Article Title: Heat Shock Factor Regulation of Antimicrobial Peptides Expression Suggests a Conserved Defense Mechanism Induced by Febrile Temperature in Arthropods
    Article Snippet: .. Drosophila S2 cells (ATCC CRL 1963) were cultured at 28 °C in Schneider’s Insect Medium (Sigma-Aldrich, St. Louis, MO, USA) containing 10% fetal bovine serum (Gibco, Grand Island, NY). .. To knockdown DmHSF1 in vitro , 8 ×10 6 S2 were transfected with 15 μg dsRNA in a 6-well plate by using FuGENE ® HD Transfection Reagent (Promega Shanghai, China), and transfected cells were incubated at 27 °C for 3 days before the transfection and DCV infection assays according to a previously published method ( ).

    Article Title: An extracellular vesicle targeting ligand that binds to Arc proteins and facilitates Arc transport in vivo
    Article Snippet: Samples were mounted in VECTASHIELD (Vector Laboratories) and analyzed on a Zeiss LSM 880. .. Cell culture and preparation of EVs and cell lysates EVs and cell lysates were prepared from S2 cells (ATCC, CRL- 1963) that were cultured for four days at 22°C in Schneider’s medium (Gibco) supplemented with 10% exosome- free FBS (#EXO- FBSHI- 50A- 1, SBI) to avoid contamination from Bovine serum exosomes. .. S2 cells were authenticated and confirmed to be free of mycoplasma by ATCC.

    Drosophila:

    Article Title: Mapping catalytically engaged TOP2B in neurons reveals the principles of topoisomerase action within the genome
    Article Snippet: .. Schneider’s Drosophila Line 2 (S2) , ATCC , CRL-1963. ..



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