spectra max plus plate reader (Danaher Inc)

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Danaher Inc spectra max plus plate reader
Spectra Max Plus Plate Reader, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1424 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/elisa+reader/SpectraMax+ABS+and+ABS+Plus/pmc03395654-232-8-13
Average 96 stars, based on 1424 article reviews

spectra max plus plate reader - by Bioz Stars, 2026-09

96/100 stars

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Activity Assay:

Article Title: Rational scaffold design mitigates mitochondrial complex I off-target inhibition of bifunctional degraders
Article Snippet: .. Complex I activity was measured in bovine mitochondrial membranes by monitoring the rate of NADH:O 2 oxidoreduction spectrophotometrically using a SpectraMax Plus 348 96-well plate reader (Molecular Devices). .. Membranes (10 μg·mL -1 ) were assayed in the presence of 200 μM NADH and 3 μM horse heart cytochrome c (Sigma Aldrich) in buffer containing 10 mM MOPS (pH 7.5) and 50 mM KCl.

Article Title: A Prussian Blue Nanozyme-Adjuvanted Vaccine Presenting Phosphocholine Antigens for Induction of Immunotolerance in Inflammatory Bowel Disease.
Article Snippet: .. The absorbance of the color reaction at 650 nm was measured at specific time points using a SpectraMax Absorbance Reader (CMAx Plus, Molecular devices, USA) to quantify the POD-like activity. ..

Incubation:

Article Title: Comprehensive identification of the genes involved in the expression of Siglec-15 ligands on osteoclast precursors.
Article Snippet: .. The wells were blocked in 100 μL assay buffer (1% BSA, 0.02% sodium azide, D-PBS) at room temperature for 1 h. Siglec-15 (WT or R143A)-Fc (1 μg/mL, prepared in-house as described above) and alkaline phosphatase (AP)-conjugated anti-hIgG-Fc antibody (1 μg/mL, Jackson ImmunoResearch, #109-055-098) were mixed in assay buffer, applied at 100 μL per well and incubated at room temperature for 1 h. The AP substrate (100 mM Na 2 CO 3 (Millipore Sigma, #71345), 10 mM p-nitrophenyl phosphate (Millipore Sigma, #N3254), and 1 mM MgCl 2 (Millipore Sigma, #M2393)) was applied at 100 μL per well, and the absorbance at 405 nm was measured using a SpectraMax Paradigm Multi-Mode Detection platform (Molecular Devices). .. To evaluate the influence of LRPAP1 on the Siglec15-LRP1 cluster III interaction, recombinant mouse LRPAP1 (rmLRPAP1, R&D systems, #4480-LR) at 0.5 or 1 μg/mL in assay buffer, 100 μL/well was added to the wells on which LRP1 cluster III was immobilized, and incubated at room temperature for 1 h before probing with Siglec-15-Fc and AP-conjugated anti-hIgG Fc antibody.

Article Title: Effects of human immunoglobulins on Cryptococcus neoformans morphology and proteome.
Article Snippet: After washing three times with PBS containing 0.05% of Tween-20 (vol/vol) using an AquaMax 2000 microplate washer (Molecular Devices), plates were incubated with anti-human IgM, IgG, or IgA antibodies conjugated to alkaline phosphatase (AP) (Southern Biotech) for 1 h at 37°C. .. The plates were washed, incubated with p-nitrophenyl phosphate (PNPP) at room temperature (RT), and the resulting color was measured by a SpectraMax ABS reader (Molecular Devices) at 405 nm. .. Cn var. grubii H99 cells (heretofore Cn) or acapsular mutant cap59 (heretofore cap59) were cultured in Sabouraud (BD Biosciences) broth for 48 h at 30°C in a rotatory shaker at 150 rpm and seeded onto Sabouraud agar plates.

Luminescence Assay:

Article Title: Identification of Novel Small Molecule Compounds with Readthrough Activity in Nagashima-Type Palmoplantar Keratosis
Article Snippet: .. Second, cell viability was measured using the CellTiter-Glo® 2.0 luminescence assay, in which treated cells were equilibrated to room temperature, mixed with an equal volume of CellTiter-Glo reagent to induce lysis, and the resulting ATP-dependent luminescent signal was recorded using a SpectraMax Paradigm reader. ..

Lysis:

Microplate Reader Absorbance Measurement:

Sterility:

Article Title: Unique Bidentate Chelators of Functionalized Heterocyclic Fluoroquinolones with Dual Anti-Inflammatory and Selective Cytotoxic Effects via a C7-C8 Ethylene Diamine Bridge.
Article Snippet: School of Pharmacy, University of Jordan, Queen Ranya Street, Amman, 11942, Jordan; School of Pharmacy, AL-Zaytoonah University of Jordan, Amman, JORDAN; Faculty of Nursing/Head of Allied Health, Al-Balqa Applied University, Amman, Jordan; Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid P. O.. Box 3030, Jordan; College of Pharmacy, Bilad Alrafidain University, Diyala, Iraq; Department of Basic Sciences and Humanities, Faculty of Science, The American University of Madaba, Jordan; University of Applied Science and Arts Dortmund, FH Dortmund, Dortmund, Germany; Cell Therapy Center, The University of Jordan, Amman 11942, Jordan

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