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electrotransfer solution  (Solarbio Inc)

 
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    Solarbio Inc electrotransfer solution
    Electrotransfer Solution, supplied by Solarbio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/electrotransfer+solution/electrotransfer+solution/pm37505544-89-15-17
    Average 90 stars, based on 1 article reviews
    electrotransfer solution - by Bioz Stars, 2026-09
    90/100 stars

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    Electrotransfer:

    Article Title: PTEN Overexpression Alters Autophagy Levels and Slows Sodium Arsenite-Induced Hepatic Stellate Cell Fibrosis.
    Article Snippet: Protein samples (30 μg) were separated using sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) with electrophoresis solution (Solarbio Co., Beijing, China). .. The proteins were further transferred to 0.22-μm polyvinylidene fluoride membranes (Sangon Biotech, Shanghai, China) with electrotransfer solution (Solarbio Co., Beijing, China). .. After blocking in 5% skimmed milk for 1 h at 37 ◦C, the blots were incubated overnight at 4 ◦C with the following primary antibodies: LC3 (1:1000) (Proteintech, Rosemont, IL, USA, 14600-1-AP), Beclin-1 (dilution 1:2000) (Bioss, Woburn, MA, USA, bs1353R), α-smooth muscle actin (α-SMA) (1:1000) (Abcam Co., Cambridge, UK), Collagen I (1:1000) (Abcam Co., UK, ab124964), HES1 (1:1000) (Abcam Co, UK), PTEN (1:1000) (CST, 11988S), NOTCH1 (1:500) (Abcam Co., UK, ab52627), GAPDH (1:10,000) (Abcam Co., UK), and β-actin (1:10,000) (Abcam Co., UK).



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