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blocking buffer  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology blocking buffer
    Blocking Buffer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 11926 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/electron+microscopy+fixation+buffer/Triton+X-100/pmc06198093-68-32-68
    Average 96 stars, based on 11926 article reviews
    blocking buffer - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Biomimetic scaffolds mimic human subretinal debris for preclinical testing of prosthetic vision
    Article Snippet: .. Samples were incubated for 48 h at room temperature with mouse anti-PKCα antibody (Santa Cruz Biotechnology, sc-8393) diluted in PBS containing 5% BSA and 0.5% Triton X-100. .. After washing with PBS containing 0.1% Tween-20, samples were incubated for 48 h at room temperature with Cy3-conjugated donkey anti-mouse secondary antibody (Jackson ImmunoResearch, 715-165-150).

    Article Title: Attractin-like protein 1 is an essential partner of MC4R to regulate body weight
    Article Snippet: Samples were embedded in Tissue-Tek OCT compound (Sakura Finetechical Co., Ltd., Tokyo) and sectioned at 35 μm thickness using a microtome (Leica Biosystems, Buffalo Grove, IL, USA). .. Free-floating sections were washed in PBS two times and incubated with blocking buffer containing 5% normal goat serum in PBS with 0.5% Triton X-100 for 1 h followed by rabbit polyclonal anti-cFos antibody (sc52, Santa Cruz Biotechnology, TX, USA) in blocking solution (1:1000) overnight at 4 °C. .. After washing with PBS containing 0.1% Tween-20 (PBST), the sections were incubated in 1:500 Alexa Fluor 594 goat anti-rabbit IgG (R37117, Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature.

    Article Title: Magnolol-encapsulated PLGA nanoparticles attenuate inflammatory responses and oxidative stress in human peritoneal macrophages isolated from patients with cirrhosis
    Article Snippet: .. Briefly, after 4 h and 24 h of incubation, the cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 2% bovine serum albumin and labeled with 1:100 dilution of LAMP1 antibody (H4A3, Santa Cruz Biotechnology, USA). .. After washing, anti-Mouse IgG secondary antibody conjugated with Alexa Fluor 488 was added to the cells according to the manufacturer’s instructions (Thermo Fisher Scientific, USA).

    Article Title: Beta‐Arrestin‐2 Binds to Nucleolar Organizing Regions With P2X7 Receptor Stimulation and During Cell Division
    Article Snippet: .. Cells, grown in chamber slides, were washed four times with PBS at room temperature, fixed with 2% (wt/vol) paraformaldehyde in PBS for 20 min and permeabilized with 0.1% (wt/vol) Triton X- 100 in PBS for 15 min. After incubation for 15 min in the blocking buffer (1% normal goat serum, 0.2% [vol/vol] Triton X- 100 in PBS), cells were incubated for 1 h at room temperature in 0.1% (wt/vol) BSA in PBS with the primary antibodies (Santa Cruz Biotechnology, Cat. Nos: Sc- 166 001, Sc- 55 486 and Sc- 13 125 for anti- fibrillarin, anti- nucleolin and anti- UBF, respectively) at a 1:50 dilution. .. After washing four times with 0.1% BSA in PBS, cells were incubated for 1 h at room temperature with Alexa Fluor 594- labeled anti- rabbit antibody (Jackson ImmunoResearch, Cat. No: 111–585- 045) at a 1:100 dilution.

    Article Title: YAP/TAZ-controlled ERK dynamics coordinate progenitor expansion and differentiation commitment
    Article Snippet: .. Primary antibodies were diluted in PBS containing 1% BSA and 0.3% Triton X-100 and incubated overnight at 4 °C: YAP (Cell Signaling Technology, D8H1X, cat. 14074, 1:1000), TAZ (Cell Signaling Technology, E8E9G, cat. 83669, 1:1000), PPARG (Santa Cruz Biotechnology, E-8, sc-7273, 1:1000), Pan-TEAD (Cell Signaling Technology, D3F7L, 1:1000), phospho-p44/42 MAPK (Thr202/Tyr204) (Cell Signaling Technology, 4370, 1:400), and total p44/42 MAPK (Cell Signaling Technology, 4696, 1:400). .. Cells were washed three times with PBS, incubated for 1.5 h with Alexa Fluor-conjugated anti-rabbit or anti-mouse secondary antibodies (Thermo Fisher Scientific, 1:1000) in PBS containing 1% BSA and 0.3% Triton X-100, then counterstained with DAPI for 30 min at room temperature and washed three times with PBS before imaging.

    Blocking Assay:

    Article Title: Attractin-like protein 1 is an essential partner of MC4R to regulate body weight
    Article Snippet: Samples were embedded in Tissue-Tek OCT compound (Sakura Finetechical Co., Ltd., Tokyo) and sectioned at 35 μm thickness using a microtome (Leica Biosystems, Buffalo Grove, IL, USA). .. Free-floating sections were washed in PBS two times and incubated with blocking buffer containing 5% normal goat serum in PBS with 0.5% Triton X-100 for 1 h followed by rabbit polyclonal anti-cFos antibody (sc52, Santa Cruz Biotechnology, TX, USA) in blocking solution (1:1000) overnight at 4 °C. .. After washing with PBS containing 0.1% Tween-20 (PBST), the sections were incubated in 1:500 Alexa Fluor 594 goat anti-rabbit IgG (R37117, Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature.

    Article Title: Beta‐Arrestin‐2 Binds to Nucleolar Organizing Regions With P2X7 Receptor Stimulation and During Cell Division
    Article Snippet: .. Cells, grown in chamber slides, were washed four times with PBS at room temperature, fixed with 2% (wt/vol) paraformaldehyde in PBS for 20 min and permeabilized with 0.1% (wt/vol) Triton X- 100 in PBS for 15 min. After incubation for 15 min in the blocking buffer (1% normal goat serum, 0.2% [vol/vol] Triton X- 100 in PBS), cells were incubated for 1 h at room temperature in 0.1% (wt/vol) BSA in PBS with the primary antibodies (Santa Cruz Biotechnology, Cat. Nos: Sc- 166 001, Sc- 55 486 and Sc- 13 125 for anti- fibrillarin, anti- nucleolin and anti- UBF, respectively) at a 1:50 dilution. .. After washing four times with 0.1% BSA in PBS, cells were incubated for 1 h at room temperature with Alexa Fluor 594- labeled anti- rabbit antibody (Jackson ImmunoResearch, Cat. No: 111–585- 045) at a 1:100 dilution.

    Labeling:

    Article Title: Magnolol-encapsulated PLGA nanoparticles attenuate inflammatory responses and oxidative stress in human peritoneal macrophages isolated from patients with cirrhosis
    Article Snippet: .. Briefly, after 4 h and 24 h of incubation, the cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 2% bovine serum albumin and labeled with 1:100 dilution of LAMP1 antibody (H4A3, Santa Cruz Biotechnology, USA). .. After washing, anti-Mouse IgG secondary antibody conjugated with Alexa Fluor 488 was added to the cells according to the manufacturer’s instructions (Thermo Fisher Scientific, USA).

    Cell-Signaling:

    Article Title: YAP/TAZ-controlled ERK dynamics coordinate progenitor expansion and differentiation commitment
    Article Snippet: .. Primary antibodies were diluted in PBS containing 1% BSA and 0.3% Triton X-100 and incubated overnight at 4 °C: YAP (Cell Signaling Technology, D8H1X, cat. 14074, 1:1000), TAZ (Cell Signaling Technology, E8E9G, cat. 83669, 1:1000), PPARG (Santa Cruz Biotechnology, E-8, sc-7273, 1:1000), Pan-TEAD (Cell Signaling Technology, D3F7L, 1:1000), phospho-p44/42 MAPK (Thr202/Tyr204) (Cell Signaling Technology, 4370, 1:400), and total p44/42 MAPK (Cell Signaling Technology, 4696, 1:400). .. Cells were washed three times with PBS, incubated for 1.5 h with Alexa Fluor-conjugated anti-rabbit or anti-mouse secondary antibodies (Thermo Fisher Scientific, 1:1000) in PBS containing 1% BSA and 0.3% Triton X-100, then counterstained with DAPI for 30 min at room temperature and washed three times with PBS before imaging.



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