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calcein am staining solution  (Beyotime)


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    Structured Review

    Beyotime calcein am staining solution
    Fig. 5. High glucose induced cardiomyocyte pyroptosis.A.Immunofluorescence was used to identify the purity of <t>NRVMs.B.Calcein-AM/Hoechst</t> 33342 staining was used to observe cardiomyocyte membrane injury caused by high glucose.C.Scanning electron microscopy was used to observe the effect of high glucose on NRVMs damage.D-G.High glucose induced increased expressions of pyroptosis related proteins in NRVMs.Values are expressed as mean±SD (n=4).*P<0.05,**P<0.01vs Control group.
    Calcein Am Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 3164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/electron+microscopy+fixation+buffer/Calcein+AM/pm38657502-108-4-8
    Average 99 stars, based on 3164 article reviews
    calcein am staining solution - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "Protective role of hydrogen sulfide against diabetic cardiomyopathy by inhibiting pyroptosis and myocardial fibrosis."

    Article Title: Protective role of hydrogen sulfide against diabetic cardiomyopathy by inhibiting pyroptosis and myocardial fibrosis.

    Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

    doi: 10.1016/j.biopha.2024.116613

    Fig. 5. High glucose induced cardiomyocyte pyroptosis.A.Immunofluorescence was used to identify the purity of NRVMs.B.Calcein-AM/Hoechst 33342 staining was used to observe cardiomyocyte membrane injury caused by high glucose.C.Scanning electron microscopy was used to observe the effect of high glucose on NRVMs damage.D-G.High glucose induced increased expressions of pyroptosis related proteins in NRVMs.Values are expressed as mean±SD (n=4).*P<0.05,**P<0.01vs Control group.
    Figure Legend Snippet: Fig. 5. High glucose induced cardiomyocyte pyroptosis.A.Immunofluorescence was used to identify the purity of NRVMs.B.Calcein-AM/Hoechst 33342 staining was used to observe cardiomyocyte membrane injury caused by high glucose.C.Scanning electron microscopy was used to observe the effect of high glucose on NRVMs damage.D-G.High glucose induced increased expressions of pyroptosis related proteins in NRVMs.Values are expressed as mean±SD (n=4).*P<0.05,**P<0.01vs Control group.

    Techniques Used: Immunofluorescence, Staining, Membrane, Electron Microscopy, Control

    Related Articles

    CCK-8 Assay:

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    Article Snippet: .. As directed by the manufacturers, cell viability was evaluated using a CCK-8 test (Glpbio, Cat. No. GK10001) and calceinAM/PI live/dead staining (Beyotime, Cat. No. C2015S). .. Human umbilical vein endothelial cells (HUVECs) and RAW264.7 macrophages were obtained from iCell Bioscience.

    Live Dead Assay:

    Article Title: A Diabetic Wound Microenvironment-Responsive HAMA-PBA/PVA Hydrogel for Localized Delivery of Netrin-1-Enriched Adipose-Derived Stem Cell-Derived Exosomes Promotes Diabetic Wound Healing through Immunomodulation and Angiogenesis.
    Article Snippet: .. As directed by the manufacturers, cell viability was evaluated using a CCK-8 test (Glpbio, Cat. No. GK10001) and calceinAM/PI live/dead staining (Beyotime, Cat. No. C2015S). .. Human umbilical vein endothelial cells (HUVECs) and RAW264.7 macrophages were obtained from iCell Bioscience.

    BIA-KA:

    Article Title: Antibacterial Performance of Defect-Enriched PtPdCuFe High-Entropy Alloy Nanozymes.
    Article Snippet: Tungsten hexacarbonyl (W(CO)6), oleylamine (OAm), glucose, polyvinylpyrrolidone (PVP), 5,5-dimethyl-1-pyrroline N-oxide (DMPO), 2,2,6,6-tetramethylpiperidine (TEMP), sodium hypochlorite (NaClO), L-methionine, riboflavin, EDTA, and nitroblue tetrazolium chloride (NBT) were purchased from Macklin (Shanghai, China). .. DMEM, fetal bovine serum (FBS), trypsin–EDTA solution, Calcein-AM, propidium iodide (PI), DCFH-DA ROS probe, BCA protein assay kit, hydrogen peroxide assay kit, TMB assay kit, MB assay kit, and DMAO bacterial fluorescent dye were purchased from Beyotime (Shanghai, China). .. LB broth and LB agar were obtained from Solarbio (Beijing, China).

    H2O2 Assay:

    Article Title: Antibacterial Performance of Defect-Enriched PtPdCuFe High-Entropy Alloy Nanozymes.
    Article Snippet: Tungsten hexacarbonyl (W(CO)6), oleylamine (OAm), glucose, polyvinylpyrrolidone (PVP), 5,5-dimethyl-1-pyrroline N-oxide (DMPO), 2,2,6,6-tetramethylpiperidine (TEMP), sodium hypochlorite (NaClO), L-methionine, riboflavin, EDTA, and nitroblue tetrazolium chloride (NBT) were purchased from Macklin (Shanghai, China). .. DMEM, fetal bovine serum (FBS), trypsin–EDTA solution, Calcein-AM, propidium iodide (PI), DCFH-DA ROS probe, BCA protein assay kit, hydrogen peroxide assay kit, TMB assay kit, MB assay kit, and DMAO bacterial fluorescent dye were purchased from Beyotime (Shanghai, China). .. LB broth and LB agar were obtained from Solarbio (Beijing, China).

    Saline:

    Article Title: Icariin Protects Against Mitochondrial Damage and Ferroptosis-Related Injury in Synovial Fibroblast-Like Synoviocytes Through SUMO1-Dependent Regulation of SIRT1.
    Article Snippet: Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by persistent synovial inflammation and progressive joint destruction, in which fibroblastlike synoviocytes (FLSs) play a central pathogenic role.. Ferroptosis and oxidative stressdriven mitochondrial dysfunction have recently been implicated in synovial cell injury during RA progression.. Sirtuin 1 (SIRT1), a nicotinamide adenine dinucleotidedependent deacetylase, is known to regulate inflammation, redox homeostasis, and cell survival; however, the upstream regulatory mechanisms controlling SIRT1 function and their relevance to ferroptosis in RA remain poorly defined.

    Incubation:

    Article Title: Icariin Protects Against Mitochondrial Damage and Ferroptosis-Related Injury in Synovial Fibroblast-Like Synoviocytes Through SUMO1-Dependent Regulation of SIRT1.
    Article Snippet: Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by persistent synovial inflammation and progressive joint destruction, in which fibroblastlike synoviocytes (FLSs) play a central pathogenic role.. Ferroptosis and oxidative stressdriven mitochondrial dysfunction have recently been implicated in synovial cell injury during RA progression.. Sirtuin 1 (SIRT1), a nicotinamide adenine dinucleotidedependent deacetylase, is known to regulate inflammation, redox homeostasis, and cell survival; however, the upstream regulatory mechanisms controlling SIRT1 function and their relevance to ferroptosis in RA remain poorly defined.

    Article Title: Effect of Microcurrent Stimulation on Cartilage Regeneration in Engineered Chondrocyte Spheroids
    Article Snippet: .. Samples were washed with PBS and incubated in working solution containing 2 μM calcein-AM and 4 μM propidium iodide using a calcein-AM/propidium iodide Live/Dead staining kit (Beyotime Biotechnology, Shanghai, China) for 20 min at 37 °C in the dark. .. Fluorescence images were captured using an inverted fluorescence microscope (IX73, Olympus, Tokyo, Japan).

    Article Title: Effect of Microcurrent Stimulation on Cartilage Regeneration in Engineered Chondrocyte Spheroids.
    Article Snippet: .. Samples were 2026 washed with PBS and incubated in working solution containing 2 μM Calcein-AM and 4 μM propidium iodide (PI) using a Calcein-AM/PI Live/Dead staining kit (Beyotime Biotechnology, Shanghai, China) for 20 min at 37°C in the dark .. Fluorescence images were captured using an inverted fluorescence microscope (IX73, Olympus, Tokyo, Japan).

    Staining:

    Article Title: Icariin Protects Against Mitochondrial Damage and Ferroptosis-Related Injury in Synovial Fibroblast-Like Synoviocytes Through SUMO1-Dependent Regulation of SIRT1.
    Article Snippet: Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by persistent synovial inflammation and progressive joint destruction, in which fibroblastlike synoviocytes (FLSs) play a central pathogenic role.. Ferroptosis and oxidative stressdriven mitochondrial dysfunction have recently been implicated in synovial cell injury during RA progression.. Sirtuin 1 (SIRT1), a nicotinamide adenine dinucleotidedependent deacetylase, is known to regulate inflammation, redox homeostasis, and cell survival; however, the upstream regulatory mechanisms controlling SIRT1 function and their relevance to ferroptosis in RA remain poorly defined.

    Article Title: Effect of Microcurrent Stimulation on Cartilage Regeneration in Engineered Chondrocyte Spheroids
    Article Snippet: .. Samples were washed with PBS and incubated in working solution containing 2 μM calcein-AM and 4 μM propidium iodide using a calcein-AM/propidium iodide Live/Dead staining kit (Beyotime Biotechnology, Shanghai, China) for 20 min at 37 °C in the dark. .. Fluorescence images were captured using an inverted fluorescence microscope (IX73, Olympus, Tokyo, Japan).

    Article Title: Identification of the miR-210-3p-EFNA3-YAP axis and its targeted modulation via biomimetic supramolecular nanoparticles to promote ischemic flap survival.
    Article Snippet: .. HUVECs were stained with a cell-permeable dye (calcein AM; Beyotime, C2012) for 30 minutes. .. ImageJ software was utilized to determine tube lengths in duplicate wells.

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    Article Title: Effect of Microcurrent Stimulation on Cartilage Regeneration in Engineered Chondrocyte Spheroids.
    Article Snippet: .. Samples were 2026 washed with PBS and incubated in working solution containing 2 μM Calcein-AM and 4 μM propidium iodide (PI) using a Calcein-AM/PI Live/Dead staining kit (Beyotime Biotechnology, Shanghai, China) for 20 min at 37°C in the dark .. Fluorescence images were captured using an inverted fluorescence microscope (IX73, Olympus, Tokyo, Japan).



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