scanning electron microscopy sem experiment s aureus atcc 6538  (ATCC)

Journal: Molecular Therapy. Nucleic Acids

Article Title: Lnc-CCNH-8 promotes immune escape by up-regulating PD-L1 in hepatocellular carcinoma

doi: 10.1016/j.omtn.2024.102125

Figure Lengend Snippet: Lnc-CCNH-8 as a potential predictive marker for immunotherapy response in HCC (A) Morphology of exosomes by electron microscopy. Scale bar: 200 nm. (B) Particle size analysis of exosomes. (C) Exosome marker proteins Alix, CD63, and CD81 were detected using western blot. (D) Expression level of Lnc-CCNH-8 (detected using RT-PCR) in plasma-derived exosomes from HCC patients (n = 79) and healthy individuals (n = 30). ∗∗p < 0.01. (E) Expression level of Lnc-CCNH-8 (detected using RT-PCR) under therapeutic efficacy. PD, progressive disease; CR, complete response; PR, partial response; SD, stable disease. ∗p < 0.05. (F) Schematic diagram: Lnc-CCNH-8 promotes immune escape by up-regulating PD-L1 via dual mechanisms in hepatocellular carcinoma.

Article Snippet: The exosome was suspended in PBS and divided into two parts; one was used for the electron microscopy experiment (Servicebio) and the other was analyzed for particle size using a particle size analyzer (Malvern Zetasizer Nano-ZS 300).

Techniques: Marker, Electron Microscopy, Particle Size Analysis, Western Blot, Expressing, Reverse Transcription Polymerase Chain Reaction, Clinical Proteomics, Derivative Assay, Drug discovery