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anti phospho pi3 kinase p85 (Proteintech)

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Proteintech anti phospho pi3 kinase p85
Anti Phospho Pi3 Kinase P85, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/elastase/Elafin%2FSkalp+Antibody/pmc12191257-32-49-80
Average 94 stars, based on 51 article reviews

anti phospho pi3 kinase p85 - by Bioz Stars, 2026-11

94/100 stars

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Article Title: Enterohemorrhagic Escherichia coli targets Annexin A6 and ATG16L1 to inhibit autophagy and induce inflammation
Article Snippet: monoclonal mouse anti-PI3 Kinase p85α , Proteintech , 60225 , 1:1000.

Recombinant:

Article Title: Circ_0005372 targets the miR-153-3p/ITGB3 axis to stimulate the PI3K/AKT signaling pathway to facilitate the occurrence and development of congenital heart disease and pulmonary arterial hypertension in children.
Article Snippet: Background: Congenital heart disease (CHD) is the most common and highest incidence of congenital malformations in newborn infants.. Pulmonary arterial hypertension (PAH) is the most serious complication associated with CHD.. It is a great threat to the safety and health of newborns.

Article Title: Gexia Zhuyu Decoction Alleviates Carbon Tetrachloride-Induced Hepatic Fibrosis in Mice: Mechanistic Insights from Integrated Network Analysis and Experimental Validation Analysis
Article Snippet: Gexia Zhuyu Decoction (GXZYD) is a classic Chinese medicine formula with significant efficacy in alleviating fibrosis.. Remarkably, despite the established link between anti angiogenesis, the PI3K/AKT signaling pathway, and the advancement of hepatic fibrosis (HF), it is still uncertain whether GXZYD achieves its anti fibrotic effects by regulating these pathways.. This study aimed to verify the potential mechanism by which GXZYD fights against HF through network analysis and experiments.

Incubation:

Article Title: Embryonic stem cell-derived small extracellular vesicles alleviate lead-induced cochlear spiral ganglion neurons injury by activating PI3 K/AKT signaling pathway
Article Snippet: .. The membranes were blocked with TBST containing 5% bovine serum albumin for 2 h and then incubated with TSG101 polyclonal antibody (Proteintech, 282283, 1:1000, CHINA), CD81 monoclonal antibody (Proteintech, 66866, 1:1000, CHINA), CALNEXIN polyclonal antibody (Proteintech, 10427, 1:1000, CHINA), PI3 kinase p110 alpha monoclonal antibody (Proteintech, 670071, 1:1000, CHINA), AKT rabbit polyclonal antibody (Proteintech, 10176, 1:1000, CHINA), phospho-AKT rabbit monoclonal antibody (Proteintech, 66444, 1:1000, CHINA), mTOR rabbit polyclonal antibody (Cell Signaling Technology, 2972S, 1:1000, USA), p-mTOR rabbit polyclonal antibody (Cell Signaling Technology, 2974 T, 1:1000, USA), Bcl-2 rabbit polyclonal antibody (Proteintech, 26593, 1:1000, CHINA), BAX rabbit polyclonal antibody (Proteintech, 50599, 1:1000, CHINA), cleaved caspase-3 rabbit monoclonal antibody (Cell Signaling Technology, 9664 T, 1:1000, USA), or ACTIN mouse monoclonal antibody (Zen Bioscience, 200068, 1:10,000, CHINA) at 4°C overnight. .. Thereafter, the membranes were incubated with goat anti-rabbit IgG (H + L)-HRP (1:10,000, Abcam, USA) or goat anti-mouse IgG (H + L)-HRP (1:10,000, Abcam, USA) for 1 h. Finally, the protein bands were visualized with ECL reagent using the Azure Biosystems C300 (Azure Biotech, C300, USA).

Article Title: Single-cell analysis identifies PI3+S100A7+keratinocytes in early cervical squamous cell carcinoma with HPV infection
Article Snippet: .. The slides were then blocked for 10 min before incubation with one of the following primary antibodies for 1 h to perform immunofluorescence staining: Elafin/Skalp (1:50, 15963-1-AP, Proteintech), S100A7 (1:3000, ER65940, Huabio), CD68 (Ready-to-use, PA014, Abcam), CD163 (Ready-to-use, PA059, Abcam), and HLA–DRA (1:500, ET1610-66, Huabio). .. After washing with tris-buffered saline with tween (TBST), the slides were treated with the secondary antibody (K8000, DAKO, Santa Clara, CA, USA) for 10 min, after which the Opal dye diluent was added and incubated at room temperature for 10 min. Microwave treatment was then applied to remove the antibody complexes.

Article Title: Single-cell analysis identifies PI3+S100A7+ keratinocytes in early cervical squamous cell carcinoma with HPV infection
Article Snippet: .. The slides were then blocked for 10 min before incubation with one of the following primary antibodies for 1 h to perform immunofluorescence staining: Elafin/Skalp (1:50, 15963- 1-AP, Proteintech), S100A7 (1:3000, ER65940, Huabio), CD68 (Ready-to-use, PA014, Abcam), CD163 (Ready-touse, PA059, Abcam), and HLA–DRA (1:500, ET1610-66, Huabio). .. After washing with tris-buffered saline with tween (TBST), the slides were treated with the secondary antibody (K8000, DAKO, Santa Clara, CA, USA) for 10 min, after which the Opal dye diluent was added and incubated at room temperature for 10 min. Microwave treatment was then applied to remove the antibody complexes.

Immunofluorescence:

Article Title: Single-cell analysis identifies PI3+S100A7+ keratinocytes in early cervical squamous cell carcinoma with HPV infection
Article Snippet: .. The slides were then blocked for 10 min before incubation with one of the following primary antibodies for 1 h to perform immunofluorescence staining: Elafin/Skalp (1:50, 15963- 1-AP, Proteintech), S100A7 (1:3000, ER65940, Huabio), CD68 (Ready-to-use, PA014, Abcam), CD163 (Ready-touse, PA059, Abcam), and HLA–DRA (1:500, ET1610-66, Huabio). .. After washing with tris-buffered saline with tween (TBST), the slides were treated with the secondary antibody (K8000, DAKO, Santa Clara, CA, USA) for 10 min, after which the Opal dye diluent was added and incubated at room temperature for 10 min. Microwave treatment was then applied to remove the antibody complexes.

Staining:

Article Title: Single-cell analysis identifies PI3+S100A7+ keratinocytes in early cervical squamous cell carcinoma with HPV infection
Article Snippet: .. The slides were then blocked for 10 min before incubation with one of the following primary antibodies for 1 h to perform immunofluorescence staining: Elafin/Skalp (1:50, 15963- 1-AP, Proteintech), S100A7 (1:3000, ER65940, Huabio), CD68 (Ready-to-use, PA014, Abcam), CD163 (Ready-touse, PA059, Abcam), and HLA–DRA (1:500, ET1610-66, Huabio). .. After washing with tris-buffered saline with tween (TBST), the slides were treated with the secondary antibody (K8000, DAKO, Santa Clara, CA, USA) for 10 min, after which the Opal dye diluent was added and incubated at room temperature for 10 min. Microwave treatment was then applied to remove the antibody complexes.

Image Search Results

Single-cell transcriptomic analysis uncovers macrophages as a key immune cell population in DMED. ( A ) t-SNE visualizing the annotated cell populations across 60,232 cells in corpus cavernosum from 5 patients with DMED and 3 males with normal erections. ( B ) Bubble diagram showing the expression of the used marker genes across the cell populations. ( C ) t-SNE visualizing the cell population distribution in DMED and control groups, respectively. ( D ) The immune activity score of the cell populations based on the immune-related genes. ( E ) Comparison of the immune activity score of the cell populations between DMED and control groups. ( F and G ) Macrophages-mediated communication network in ( F ) DMED and ( G ) control groups. ( H and I ) Ligand-receptor pairs in macrophages-mediated communications in ( H ) DMED and ( I ) control groups. ( J ) Volcano diagram depicting the DEGs in macrophages between DMED and control groups. ( K and L ) Biological processes and KEGG pathways enriched by the DEGs. **, P<0.01; ***, P<0.001 derived from Wilcoxon test.

Journal: Journal of Inflammation Research

Article Title: HMGCS2 Palmitoylation Promotes Diabetes-Induced Erectile Dysfunction Through PPARα-Mediated Macrophage Extracellular Trap Formation

doi: 10.2147/JIR.S623377

Figure Lengend Snippet: Single-cell transcriptomic analysis uncovers macrophages as a key immune cell population in DMED. ( A ) t-SNE visualizing the annotated cell populations across 60,232 cells in corpus cavernosum from 5 patients with DMED and 3 males with normal erections. ( B ) Bubble diagram showing the expression of the used marker genes across the cell populations. ( C ) t-SNE visualizing the cell population distribution in DMED and control groups, respectively. ( D ) The immune activity score of the cell populations based on the immune-related genes. ( E ) Comparison of the immune activity score of the cell populations between DMED and control groups. ( F and G ) Macrophages-mediated communication network in ( F ) DMED and ( G ) control groups. ( H and I ) Ligand-receptor pairs in macrophages-mediated communications in ( H ) DMED and ( I ) control groups. ( J ) Volcano diagram depicting the DEGs in macrophages between DMED and control groups. ( K and L ) Biological processes and KEGG pathways enriched by the DEGs. **, P<0.01; ***, P<0.001 derived from Wilcoxon test.

Article Snippet: Primary antibodies included: HMGCS2 (ab305,227; Abcam, USA), peptidyl arginine deiminase 4 (PAD4; 17,373-1-AP; Proteintech, China), macrophage elastase (ME; 22,989-1-AP; Proteintech), MPO (22,225-1-AP; Proteintech), GAPDH (T0004; Affinity, USA), β-Tubulin (66,240-1-Ig; Proteintech), and PPARα (66,826-1-Ig; Proteintech).

Techniques: Single Cell, Analysis, Immune Cell, Expressing, Marker, Control, Activity Assay, Comparison, Derivative Assay

HG and PA treatments induce high expression of HMGCS2 and MET formation in macrophages. ( A and B ) Volcano and heatmap diagrams depicting the DEGs in corpus cavernosum between DMED rats and controls. ( C ) Heatmap visualizing the top 20 up- and downregulated DEGs in DMED, respectively. ( D and E ) Biological processes and KEGG pathways enriched by the DEGs. ( F ) Visualization of PPAR signaling pathway enriched by the DEGs. ( G ) qRT-PCR for detecting the mRNA expression of HMGCS2 in control RAW264.7 macrophages and those with treatments of 25 mmol/L HG and 60 μM PA for 48 h. ( H ) Representative Western blot of HMGCS2, PAD4, ME, and MPO in control and HG- and PA-induced RAW264.7 macrophages. ( I-L ) Quantification of the expression levels of HMGCS2, PAD4, ME, and MPO in control and HG- and PA-induced RAW264.7 macrophages. ***, P<0.001; ****, P<0.0001 derived from Student’s t test.

Journal: Journal of Inflammation Research

Article Title: HMGCS2 Palmitoylation Promotes Diabetes-Induced Erectile Dysfunction Through PPARα-Mediated Macrophage Extracellular Trap Formation

doi: 10.2147/JIR.S623377

Figure Lengend Snippet: HG and PA treatments induce high expression of HMGCS2 and MET formation in macrophages. ( A and B ) Volcano and heatmap diagrams depicting the DEGs in corpus cavernosum between DMED rats and controls. ( C ) Heatmap visualizing the top 20 up- and downregulated DEGs in DMED, respectively. ( D and E ) Biological processes and KEGG pathways enriched by the DEGs. ( F ) Visualization of PPAR signaling pathway enriched by the DEGs. ( G ) qRT-PCR for detecting the mRNA expression of HMGCS2 in control RAW264.7 macrophages and those with treatments of 25 mmol/L HG and 60 μM PA for 48 h. ( H ) Representative Western blot of HMGCS2, PAD4, ME, and MPO in control and HG- and PA-induced RAW264.7 macrophages. ( I-L ) Quantification of the expression levels of HMGCS2, PAD4, ME, and MPO in control and HG- and PA-induced RAW264.7 macrophages. ***, P<0.001; ****, P<0.0001 derived from Student’s t test.

Techniques: Expressing, Quantitative RT-PCR, Control, Western Blot, Derivative Assay

HG and PA treatments induce ROS over-production, oxidative stress, and mitochondrial dysfunction in macrophages. ( A ) Representative intracellular ROS photographs using DCFH-DA fluorescence probe in control RAW264.7 macrophages and those with treatments of 25 mmol/L HG and 60 μM PA for 48 h. Scale bars, 50 μm. ( B ) Quantification of fluorescence intensity of ROS in control and HG- and PA-induced RAW264.7 macrophages. ( C and D ) Measurement of MDA and GSH levels in control and HG- and PA-induced RAW264.7 macrophages. ( E ) Representative JC-1 staining photographs in control and HG- and PA-induced RAW264.7 macrophages. Green fluorescence represents that MMP is low and JC-1 exists in the cytoplasm in the form of monomer. Red fluorescence represents that MMP is high and JC-1 polymer is formed. Scale bars, 50 μm. ( F ) Calculation of a ratio of green fluorescence and red fluorescence for quantification MMP changes. ( G ) Detection of ATP in control and HG- and PA-induced RAW264.7 macrophages. ( H ) TEM for investigating the changes in mitochondrial morphology in control and HG- and PA-induced RAW264.7 macrophages. Scale bars, 500 nm. **, P<0.01; ***, P<0.001; ****, P<0.0001 derived from Student’s t test.

Journal: Journal of Inflammation Research

Article Title: HMGCS2 Palmitoylation Promotes Diabetes-Induced Erectile Dysfunction Through PPARα-Mediated Macrophage Extracellular Trap Formation

doi: 10.2147/JIR.S623377

Figure Lengend Snippet: HG and PA treatments induce ROS over-production, oxidative stress, and mitochondrial dysfunction in macrophages. ( A ) Representative intracellular ROS photographs using DCFH-DA fluorescence probe in control RAW264.7 macrophages and those with treatments of 25 mmol/L HG and 60 μM PA for 48 h. Scale bars, 50 μm. ( B ) Quantification of fluorescence intensity of ROS in control and HG- and PA-induced RAW264.7 macrophages. ( C and D ) Measurement of MDA and GSH levels in control and HG- and PA-induced RAW264.7 macrophages. ( E ) Representative JC-1 staining photographs in control and HG- and PA-induced RAW264.7 macrophages. Green fluorescence represents that MMP is low and JC-1 exists in the cytoplasm in the form of monomer. Red fluorescence represents that MMP is high and JC-1 polymer is formed. Scale bars, 50 μm. ( F ) Calculation of a ratio of green fluorescence and red fluorescence for quantification MMP changes. ( G ) Detection of ATP in control and HG- and PA-induced RAW264.7 macrophages. ( H ) TEM for investigating the changes in mitochondrial morphology in control and HG- and PA-induced RAW264.7 macrophages. Scale bars, 500 nm. **, P<0.01; ***, P<0.001; ****, P<0.0001 derived from Student’s t test.

Techniques: Fluorescence, Control, Staining, Polymer, Morphology, Derivative Assay

Pharmacological suppression of protein palmitoylation downregulates HMGCS2 palmitoylation and expression and hinders MET formation in HG- and PA-induced macrophages. ( A ) ABE assay for measuring HMGCS2 palmitoylation in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment, in the presence or absence of HAM. ( B ) Co-IP assay for detecting the interaction of HMGCS2 with PPARα in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. ( C ) qRT-PCR for measuring the mRNA level of HMGCS2 in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. ( D ) Representative Western blot of HMGCS2, PAD4, ME, and MPO in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. ( E–H ) Quantification of the protein expression levels of HMGCS2, PAD4, ME, and MPO in the above macrophages. **, P<0.01; ***, P<0.001; ****, P<0.0001 derived from one-way ANOVA followed by Tukey’s post hoc test.

Journal: Journal of Inflammation Research

Article Title: HMGCS2 Palmitoylation Promotes Diabetes-Induced Erectile Dysfunction Through PPARα-Mediated Macrophage Extracellular Trap Formation

doi: 10.2147/JIR.S623377

Figure Lengend Snippet: Pharmacological suppression of protein palmitoylation downregulates HMGCS2 palmitoylation and expression and hinders MET formation in HG- and PA-induced macrophages. ( A ) ABE assay for measuring HMGCS2 palmitoylation in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment, in the presence or absence of HAM. ( B ) Co-IP assay for detecting the interaction of HMGCS2 with PPARα in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. ( C ) qRT-PCR for measuring the mRNA level of HMGCS2 in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. ( D ) Representative Western blot of HMGCS2, PAD4, ME, and MPO in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. ( E–H ) Quantification of the protein expression levels of HMGCS2, PAD4, ME, and MPO in the above macrophages. **, P<0.01; ***, P<0.001; ****, P<0.0001 derived from one-way ANOVA followed by Tukey’s post hoc test.

Techniques: Expressing, Control, Co-Immunoprecipitation Assay, Quantitative RT-PCR, Western Blot, Derivative Assay

Pharmacological suppression of protein palmitoylation attenuates HG- and PA-induced ROS over-production, oxidative stress, and mitochondrial dysfunction in macrophages. ( A ) Representative intracellular ROS photographs by the use of DCFH-DA fluorescence probe in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. Scale bars, 50 μm. ( B ) Quantification of fluorescence intensity of ROS in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. ( C and D ) Measurement of MDA and GSH levels in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. ( E ) Representative JC-1 staining photographs in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. Green fluorescence represents that MMP is low and JC-1 exists in the cytoplasm in the form of monomer. Red fluorescence represents that MMP is high and JC-1 polymer is formed. Scale bars, 50 μm. ( F ) Calculation of a ratio of green fluorescence and red fluorescence for quantifying MMP changes. ( G ) Detection of ATP in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. ( H ) TEM for investigating the changes in mitochondrial morphology in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. Scale bars, 500 nm. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001 derived from one-way ANOVA followed by Tukey’s post hoc test.

Journal: Journal of Inflammation Research

Article Title: HMGCS2 Palmitoylation Promotes Diabetes-Induced Erectile Dysfunction Through PPARα-Mediated Macrophage Extracellular Trap Formation

doi: 10.2147/JIR.S623377

Figure Lengend Snippet: Pharmacological suppression of protein palmitoylation attenuates HG- and PA-induced ROS over-production, oxidative stress, and mitochondrial dysfunction in macrophages. ( A ) Representative intracellular ROS photographs by the use of DCFH-DA fluorescence probe in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. Scale bars, 50 μm. ( B ) Quantification of fluorescence intensity of ROS in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. ( C and D ) Measurement of MDA and GSH levels in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. ( E ) Representative JC-1 staining photographs in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. Green fluorescence represents that MMP is low and JC-1 exists in the cytoplasm in the form of monomer. Red fluorescence represents that MMP is high and JC-1 polymer is formed. Scale bars, 50 μm. ( F ) Calculation of a ratio of green fluorescence and red fluorescence for quantifying MMP changes. ( G ) Detection of ATP in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. ( H ) TEM for investigating the changes in mitochondrial morphology in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. Scale bars, 500 nm. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001 derived from one-way ANOVA followed by Tukey’s post hoc test.

Techniques: Fluorescence, Control, Staining, Polymer, Morphology, Derivative Assay

Palmitoylation inhibitor CMA attenuates MET formation and macrophage infiltration in corpus cavernosum of DMED model rats. ( A-D ) Detection of levels of cfDNA, MPO, NE, and PAD4 in corpus cavernosum of control rats, DMED model rats, and DMED model rats with CMA administration. ( E ) Representative IHC staining of MPO, NE, and PAD4 in corpus cavernosum of control rats, DMED model rats, and DMED model rats with CMA administration. Scale bars, 50 μm. ( F–H ) Quantification of the protein expression levels of MPO, NE, and PAD4 in corpus cavernosum of control rats, DMED model rats, and DMED model rats with CMA administration. ( I ) Representative flow cytometry of CD68 + , CD11b + , and F4/80 + macrophages in corpus cavernosum of control rats, DMED model rats, and DMED model rats with CMA administration. ( J ) Quantification of the ratios of CD68 + , CD11b + , and F4/80 + macrophages in corpus cavernosum of control rats, DMED model rats, and DMED model rats with CMA administration. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001 derived from one-way ANOVA followed by Tukey’s post hoc test.

Journal: Journal of Inflammation Research

Article Title: HMGCS2 Palmitoylation Promotes Diabetes-Induced Erectile Dysfunction Through PPARα-Mediated Macrophage Extracellular Trap Formation

doi: 10.2147/JIR.S623377

Figure Lengend Snippet: Palmitoylation inhibitor CMA attenuates MET formation and macrophage infiltration in corpus cavernosum of DMED model rats. ( A-D ) Detection of levels of cfDNA, MPO, NE, and PAD4 in corpus cavernosum of control rats, DMED model rats, and DMED model rats with CMA administration. ( E ) Representative IHC staining of MPO, NE, and PAD4 in corpus cavernosum of control rats, DMED model rats, and DMED model rats with CMA administration. Scale bars, 50 μm. ( F–H ) Quantification of the protein expression levels of MPO, NE, and PAD4 in corpus cavernosum of control rats, DMED model rats, and DMED model rats with CMA administration. ( I ) Representative flow cytometry of CD68 + , CD11b + , and F4/80 + macrophages in corpus cavernosum of control rats, DMED model rats, and DMED model rats with CMA administration. ( J ) Quantification of the ratios of CD68 + , CD11b + , and F4/80 + macrophages in corpus cavernosum of control rats, DMED model rats, and DMED model rats with CMA administration. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001 derived from one-way ANOVA followed by Tukey’s post hoc test.

Techniques: Control, Immunohistochemistry, Expressing, Flow Cytometry, Derivative Assay

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