mouse anti eif4e2 (Proteintech)
Structured Review
Mouse Anti Eif4e2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eif4e2/EIF4E2+Antibody/pm41315768-681-38-36
Average 93 stars, based on 14 article reviews
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Blocking Assay:Article Title: WDR4 drives tumour-associated macrophage reprogramming and tumour progression via selective translation and membrane cholesterol efflux. Article Snippet: Tumour-associated macrophages (TAMs) play a pivotal role in shaping the immune microenvironment of hepatocellular carcinoma (HCC), influencing tumour progression and immunotherapy response.. WDR4, a tRNA-binding cofactor of the N-methylguanosine (mG) methyltransferase complex, remains poorly understood in its independent functions.. Here we show that WDR4 is significantly upregulated in HCC-associated TAMs and correlates with poor prognosis. Article Title: α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation. Article Snippet: Equal proteins were loaded onto 12% SDSPAGE gels and electrotransferred onto nitrocellulose membranes (Merck Millipore, Billerica, MA, USA). .. After blocking in 5% non-fat dry milk for 2 h, the membranes were incubated with the primary antibody at 4 °C overnight: FUT4 (19497-1-AP), PCNA (10205-2-AP), Cyclin D1 (26939-1- AP), Cyclin E1 (11554-1-AP), MMP-2 (10373-2-AP), MMP-9 (10375-2-AP), TIMP1 (16644-1-AP), TIMP2 (17353-1-AP), MEST (11118-1-AP), eIF4E2 (12227-1-AP), GIGYF2 (24790-1-AP) and GAPDH (10494-1-AP) (Proteintech, Wuhan, China); 4E-T (sc-134233, Santa Cruz, USA); LeY (ab-3359, Abcam, USA); biotinylated LTL (B-1325-2, Vector Laboratories, California, USA). .. After washing, the membranes were incubated with HRP-labeled goat antirabbit IgG (SA00001-2), HRP- HRP-labeled streptavidin (SA00001-0) or labeled goat anti-mouse IgG (SA00001-1) (Proteintech, Wuhan, China) for 1 h. The visualize immunoreactivity bands were detected with ECL detection system (Bio-Rad, USA). Article Title: α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation Article Snippet: Equal proteins were loaded onto 12% SDS-PAGE gels and electrotransferred onto nitrocellulose membranes (Merck Millipore, Billerica, MA, USA). .. After blocking in 5% non-fat dry milk for 2 h, the membranes were incubated with the primary antibody at 4 °C overnight: FUT4 (19497-1-AP), PCNA (10205-2-AP), Cyclin D1 (26939-1-AP), Cyclin E1 (11554-1-AP), MMP-2 (10373-2-AP), MMP-9 (10375-2-AP), TIMP1 (16644-1-AP), TIMP2 (17353-1-AP), MEST (11118-1-AP), Article Title: Comprehensive analysis of aberrant alternative splicing and RNA binding proteins regulators associated with myocardial ischemia reperfusion injury in mice. Article Snippet: .. After blocking with 5% non-fat dry milk at room temperature, the membranes were incubated overnight at 4°C with the following primary antibodies: anti-hypoxia-inducible factor 1 A(HIF1A) (Abcam, Cambridge, UK), anti-FN1 (Abcam, Cambridge, UK), anti-CREBRF (Thermo Fisher Scientific, Waltham, MA, USA), anti-LMNA (Abcam, Cambridge, UK), Incubation:Article Title: WDR4 drives tumour-associated macrophage reprogramming and tumour progression via selective translation and membrane cholesterol efflux. Article Snippet: Tumour-associated macrophages (TAMs) play a pivotal role in shaping the immune microenvironment of hepatocellular carcinoma (HCC), influencing tumour progression and immunotherapy response.. WDR4, a tRNA-binding cofactor of the N-methylguanosine (mG) methyltransferase complex, remains poorly understood in its independent functions.. Here we show that WDR4 is significantly upregulated in HCC-associated TAMs and correlates with poor prognosis. Article Title: α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation. Article Snippet: Equal proteins were loaded onto 12% SDSPAGE gels and electrotransferred onto nitrocellulose membranes (Merck Millipore, Billerica, MA, USA). .. After blocking in 5% non-fat dry milk for 2 h, the membranes were incubated with the primary antibody at 4 °C overnight: FUT4 (19497-1-AP), PCNA (10205-2-AP), Cyclin D1 (26939-1- AP), Cyclin E1 (11554-1-AP), MMP-2 (10373-2-AP), MMP-9 (10375-2-AP), TIMP1 (16644-1-AP), TIMP2 (17353-1-AP), MEST (11118-1-AP), eIF4E2 (12227-1-AP), GIGYF2 (24790-1-AP) and GAPDH (10494-1-AP) (Proteintech, Wuhan, China); 4E-T (sc-134233, Santa Cruz, USA); LeY (ab-3359, Abcam, USA); biotinylated LTL (B-1325-2, Vector Laboratories, California, USA). .. After washing, the membranes were incubated with HRP-labeled goat antirabbit IgG (SA00001-2), HRP- HRP-labeled streptavidin (SA00001-0) or labeled goat anti-mouse IgG (SA00001-1) (Proteintech, Wuhan, China) for 1 h. The visualize immunoreactivity bands were detected with ECL detection system (Bio-Rad, USA). Article Title: α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation Article Snippet: Equal proteins were loaded onto 12% SDS-PAGE gels and electrotransferred onto nitrocellulose membranes (Merck Millipore, Billerica, MA, USA). .. After blocking in 5% non-fat dry milk for 2 h, the membranes were incubated with the primary antibody at 4 °C overnight: FUT4 (19497-1-AP), PCNA (10205-2-AP), Cyclin D1 (26939-1-AP), Cyclin E1 (11554-1-AP), MMP-2 (10373-2-AP), MMP-9 (10375-2-AP), TIMP1 (16644-1-AP), TIMP2 (17353-1-AP), MEST (11118-1-AP), Article Title: Comprehensive analysis of aberrant alternative splicing and RNA binding proteins regulators associated with myocardial ischemia reperfusion injury in mice. Article Snippet: .. After blocking with 5% non-fat dry milk at room temperature, the membranes were incubated overnight at 4°C with the following primary antibodies: anti-hypoxia-inducible factor 1 A(HIF1A) (Abcam, Cambridge, UK), anti-FN1 (Abcam, Cambridge, UK), anti-CREBRF (Thermo Fisher Scientific, Waltham, MA, USA), anti-LMNA (Abcam, Cambridge, UK), |
