eif4e full length coding sequence (Addgene inc)
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eif4e full length coding sequence
Eif4e Full Length Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eif4e+full+length+coding+sequence/pHA-eIF4E+(Plasmid+%2317343)/pmc03381142-46-2-12
Average 93 stars, based on 20 article reviews
Eif4e Full Length Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eif4e+full+length+coding+sequence/pHA-eIF4E+(Plasmid+%2317343)/pmc03381142-46-2-12
Average 93 stars, based on 20 article reviews
eif4e full length coding sequence - by Bioz Stars,
2026-10
93/100 stars
Images
1) Product Images from "The PP242 Mammalian Target of Rapamycin (mTOR) Inhibitor Activates Extracellular Signal-regulated Kinase (ERK) in Multiple Myeloma Cells via a Target of Rapamycin Complex 1 (TORC1)/ Eukaryotic Translation Initiation Factor 4E (eIF-4E)/RAF Pathway and Activation Is a Mechanism of Resistance * "
Article Title: The PP242 Mammalian Target of Rapamycin (mTOR) Inhibitor Activates Extracellular Signal-regulated Kinase (ERK) in Multiple Myeloma Cells via a Target of Rapamycin Complex 1 (TORC1)/ Eukaryotic Translation Initiation Factor 4E (eIF-4E)/RAF Pathway and Activation Is a Mechanism of Resistance
Journal: The Journal of Biological Chemistry
doi: 10.1074/jbc.M111.304626
Figure Legend Snippet: Effects on 4E-BP1/eIF-4E mediate ERK activation. In A, 8226 or MM1.S cells treated with IGF-1 (200 ng/ml) ± rapamycin or pp242 for 30 min followed by eIF-4E precipitation and subsequent immunoblot of the precipitate for eIF-4E, 4E-BP1, and eIF-4G presence. In B, similar experiment performed in 8226 cells without presence of IGF-1. In C, empty vector (EV) or HA-eIF-4E- stably transfected MM1.S cells (left panel) or 8226 cells (right panel) treated with increasing concentrations of pp242 followed by immunoblot assay for phospho-ERK, total ERK, and HA tag. In D, empty vector or HA-eIF-4E transiently transfected 8226 cells treated with increasing concentrations of pp242 followed by immunoblot assay for phosphorylated and total ERK or phosphorylated and total MEK. In E, empty vector or eIF-4E-transfected cells treated with increasing concentrations of pp242 followed by immunoprecipitation of c-RAF and assay for raf in vitro kinase activity against MEK substrate. Fold increase in kinase activity calculated as described under “Experimental Procedures.”
Techniques Used: Activation Assay, Western Blot, Plasmid Preparation, Stable Transfection, Transfection, Immunoprecipitation, In Vitro, Activity Assay
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Article Snippet: Get e-Alerts ARTICLE | February 19, 2025 Sortase-Mediated Fluorescent Labeling of eIF4E for Investigating Translation Initiation Mechanisms , and Biochemistry Cite this: Biochemistry 2025, 64, 5, 1099–1108 https://doi.org/10.1021/acs.biochem.4c00851 Copyright © 2025 American Chemical Society Request reuse permissions Cite Share Jump to Justin Pi Simpson Joseph* Article Title: Prolonging lung cancer response to EGFR inhibition by targeting the selective advantage of resistant cells Article Snippet: Cells were co-transfected with 2 μg of the CRISPR/Cas9 plasmid and 2 μg of ssODN (50 μM) using a Nucleofector II and Amaxa Nucleofector kits (Lonza) according to the manufacturer’s instructions. .. The sequence encoding the Flag tag was cloned between the HindIII and KpnI sites of the FLAG-tag:Article Title: Prolonging lung cancer response to EGFR inhibition by targeting the selective advantage of resistant cells Article Snippet: Lentiviral vectors for inducible expression of CA-MNK2 (T379D) and SLUG were generated by Gibson assembly (New England Biolabs) or standard cloning in the pTRIPZ plasmid from pTK-Slug (a gift from Robert Weinberg, Addgene plasmid # 36986) ( ) and pDONR223-MKNK2 (a gift from William Hahn & David Root, Addgene plasmid # 23735) ( ). .. The sequence encoding the Flag tag was cloned between the HindIII and KpnI sites of the Article Title: Prolonging lung cancer response to EGFR inhibition by targeting the selective advantage of resistant cells Article Snippet: Cells were co-transfected with 2 μg of the CRISPR/Cas9 plasmid and 2 μg of ssODN (50 μM) using a Nucleofector II and Amaxa Nucleofector kits (Lonza) according to the manufacturer’s instructions. .. The sequence encoding the Flag tag was cloned between the HindIII and KpnI sites of the Clone Assay:Article Title: Prolonging lung cancer response to EGFR inhibition by targeting the selective advantage of resistant cells Article Snippet: Lentiviral vectors for inducible expression of CA-MNK2 (T379D) and SLUG were generated by Gibson assembly (New England Biolabs) or standard cloning in the pTRIPZ plasmid from pTK-Slug (a gift from Robert Weinberg, Addgene plasmid # 36986) ( ) and pDONR223-MKNK2 (a gift from William Hahn & David Root, Addgene plasmid # 23735) ( ). .. The sequence encoding the Flag tag was cloned between the HindIII and KpnI sites of the Article Title: Prolonging lung cancer response to EGFR inhibition by targeting the selective advantage of resistant cells Article Snippet: Cells were co-transfected with 2 μg of the CRISPR/Cas9 plasmid and 2 μg of ssODN (50 μM) using a Nucleofector II and Amaxa Nucleofector kits (Lonza) according to the manufacturer’s instructions. .. The sequence encoding the Flag tag was cloned between the HindIII and KpnI sites of the Plasmid Preparation:Article Title: Prolonging lung cancer response to EGFR inhibition by targeting the selective advantage of resistant cells Article Snippet: Lentiviral vectors for inducible expression of CA-MNK2 (T379D) and SLUG were generated by Gibson assembly (New England Biolabs) or standard cloning in the pTRIPZ plasmid from pTK-Slug (a gift from Robert Weinberg, Addgene plasmid # 36986) ( ) and pDONR223-MKNK2 (a gift from William Hahn & David Root, Addgene plasmid # 23735) ( ). .. The sequence encoding the Flag tag was cloned between the HindIII and KpnI sites of the Article Title: Sortase-Mediated Fluorescent Labeling of eIF4E for Investigating Translation Initiation Mechanisms. 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Horseradish peroxidase (HRP), streptavidin-coated 384-well plates (white, high binding capacity; cat #15505), and SuperSignal West Pico Chemiluminescent substrate kit were purchased from Pierce. eIF4GI-HaloTag human ORF in pFN21A was purchased from Promega (protein accession ID Q04637.4). eIF4EBP1-HaloTag human ORF in pFN21A was purchased from Promega (protein accession ID Q13541.3). pFN29K His6HaloTag T7 Flexi vector and the Flexi System cloning kit were purchased from Promega. Article Title: Prolonging lung cancer response to EGFR inhibition by targeting the selective advantage of resistant cells Article Snippet: Cells were co-transfected with 2 μg of the CRISPR/Cas9 plasmid and 2 μg of ssODN (50 μM) using a Nucleofector II and Amaxa Nucleofector kits (Lonza) according to the manufacturer’s instructions. .. The sequence encoding the Flag tag was cloned between the HindIII and KpnI sites of the Expressing:Article Title: Sortase-Mediated Fluorescent Labeling of eIF4E for Investigating Translation Initiation Mechanisms. Article Snippet: Get e-Alerts ARTICLE | February 19, 2025 Sortase-Mediated Fluorescent Labeling of eIF4E for Investigating Translation Initiation Mechanisms , and Biochemistry Cite this: Biochemistry 2025, 64, 5, 1099–1108 https://doi.org/10.1021/acs.biochem.4c00851 Copyright © 2025 American Chemical Society Request reuse permissions Cite Share Jump to Justin Pi Simpson Joseph* Construct:Article Title: Sortase-Mediated Fluorescent Labeling of eIF4E for Investigating Translation Initiation Mechanisms. Article Snippet: Get e-Alerts ARTICLE | February 19, 2025 Sortase-Mediated Fluorescent Labeling of eIF4E for Investigating Translation Initiation Mechanisms , and Biochemistry Cite this: Biochemistry 2025, 64, 5, 1099–1108 https://doi.org/10.1021/acs.biochem.4c00851 Copyright © 2025 American Chemical Society Request reuse permissions Cite Share Jump to Justin Pi Simpson Joseph* Binding Assay:Article Title: Supporting Information for High-Throughput Chemical Probing of Full-Length Protein-Protein Interactions Article Snippet: TCO-PEG4-NHS was purchased from Click Chemistry Tools and used as received. .. Horseradish peroxidase (HRP), streptavidin-coated 384-well plates (white, high binding capacity; cat #15505), and SuperSignal West Pico Chemiluminescent substrate kit were purchased from Pierce. eIF4GI-HaloTag human ORF in pFN21A was purchased from Promega (protein accession ID Q04637.4). eIF4EBP1-HaloTag human ORF in pFN21A was purchased from Promega (protein accession ID Q13541.3). pFN29K His6HaloTag T7 Flexi vector and the Flexi System cloning kit were purchased from Promega. Cloning:Article Title: Supporting Information for High-Throughput Chemical Probing of Full-Length Protein-Protein Interactions Article Snippet: TCO-PEG4-NHS was purchased from Click Chemistry Tools and used as received. .. 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