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modified egfp with an 8-base insertion  (Fasmac Co Ltd)

 
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    Fasmac Co Ltd modified egfp with an 8-base insertion
    Modified Egfp With An 8 Base Insertion, supplied by Fasmac Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egfp+insert/modified+egfp+with+an+8+base+insertion/pmc11928489-151-4-9
    Average 90 stars, based on 1 article reviews
    modified egfp with an 8-base insertion - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Modification:

    Article Title: Efficiency of genome editing using modified single-stranded oligodeoxyribonucleotides in human cells
    Article Snippet: The cell culture incubator (Thermo Fisher Scientific, Inc.) was maintained at 37 ̊C with 5% of CO 2 gas concentration. .. Modified EGFP with an 8-base insertion was synthesized by Fasmac (Japan) and was amplified by PCR using the inf-EGFP5 (5′-TAGAGCTAGCGAATTATGGTGAGCAAGGGCGAGG AG-3′) and GFP-sv40polyA-pCDH-R (5′-ATTTAAATTCGAATTATAAGATACATTG ATGAGTT-3′) primer pair. .. The resulting product was ligated with Eco RI-treated plasmid pCDH-CMV-MCS-EF1-RFP-Puro (System Biosciences) through the In-Fusion reaction.

    Synthesized:

    Article Title: Efficiency of genome editing using modified single-stranded oligodeoxyribonucleotides in human cells
    Article Snippet: The cell culture incubator (Thermo Fisher Scientific, Inc.) was maintained at 37 ̊C with 5% of CO 2 gas concentration. .. Modified EGFP with an 8-base insertion was synthesized by Fasmac (Japan) and was amplified by PCR using the inf-EGFP5 (5′-TAGAGCTAGCGAATTATGGTGAGCAAGGGCGAGG AG-3′) and GFP-sv40polyA-pCDH-R (5′-ATTTAAATTCGAATTATAAGATACATTG ATGAGTT-3′) primer pair. .. The resulting product was ligated with Eco RI-treated plasmid pCDH-CMV-MCS-EF1-RFP-Puro (System Biosciences) through the In-Fusion reaction.

    Amplification:

    Article Title: Efficiency of genome editing using modified single-stranded oligodeoxyribonucleotides in human cells
    Article Snippet: The cell culture incubator (Thermo Fisher Scientific, Inc.) was maintained at 37 ̊C with 5% of CO 2 gas concentration. .. Modified EGFP with an 8-base insertion was synthesized by Fasmac (Japan) and was amplified by PCR using the inf-EGFP5 (5′-TAGAGCTAGCGAATTATGGTGAGCAAGGGCGAGG AG-3′) and GFP-sv40polyA-pCDH-R (5′-ATTTAAATTCGAATTATAAGATACATTG ATGAGTT-3′) primer pair. .. The resulting product was ligated with Eco RI-treated plasmid pCDH-CMV-MCS-EF1-RFP-Puro (System Biosciences) through the In-Fusion reaction.

    Polymerase Chain Reaction:

    Article Title: Efficiency of genome editing using modified single-stranded oligodeoxyribonucleotides in human cells
    Article Snippet: The cell culture incubator (Thermo Fisher Scientific, Inc.) was maintained at 37 ̊C with 5% of CO 2 gas concentration. .. Modified EGFP with an 8-base insertion was synthesized by Fasmac (Japan) and was amplified by PCR using the inf-EGFP5 (5′-TAGAGCTAGCGAATTATGGTGAGCAAGGGCGAGG AG-3′) and GFP-sv40polyA-pCDH-R (5′-ATTTAAATTCGAATTATAAGATACATTG ATGAGTT-3′) primer pair. .. The resulting product was ligated with Eco RI-treated plasmid pCDH-CMV-MCS-EF1-RFP-Puro (System Biosciences) through the In-Fusion reaction.



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