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egfp  (Genecopoeia)


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    Structured Review

    Genecopoeia egfp
    Egfp, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egfp+insert/Human+cell+line+HEK293T+stably+expressing+CRISPR+Cas9%2C+AAVS1+inserted%2C+single+clone/pm39739600-337-12-13
    Average 94 stars, based on 9 article reviews
    egfp - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    other:

    Article Title: Structurally related phage helicases trigger type III-A3 retron-mediated anti-phage defense across diverse tailed phage families
    Article Snippet: Two microliters of the lysed culture were used as a template for PCR reactions with primers containing adapters for Illumina sequencing preparation.

    Article Title: Structural basis for retron co-option of anti-phage ATPase-nuclease.
    Article Snippet: The products were resolved on 15% urea–polyacrylamide gel electrophoresis gels, visualized by SYBR Gold staining and imaged using the Bio-Rad ChemiDoc system.

    Article Title: Structural basis for retron co-option of anti-phage ATPase-nuclease
    Article Snippet: The reaction was stopped by the addition of RNA loading dye (Thermo Fisher), and was then heat-denatured at 95 °C for 3 min.

    Expressing:

    Article Title: Structural Basis for Retron Co-option of Anti-phage ATPase-nuclease
    Article Snippet: .. HEK293T cells constitutively expressing SpCas9 from a CBh promoter at the AAVS1 Safe Harbor locus (GeneCopoeia, SL502) were maintained in high-glucose DMEM supplemented with GlutaMax (Thermo Fisher Scientific, 10569010). .. HEK293T cells constitutively expressing SpCas9 from a CBh promoter at the AAVS1 Safe Harbor locus (GeneCopoeia, SL502) were maintained in high-glucose DMEM supplemented with GlutaMax (Thermo Fisher Scientific, 10569010).

    Article Title: Structurally related phage helicases trigger type III-A3 retron-mediated anti-phage defense across diverse tailed phage families.
    Article Snippet: An Illumina NextSeq instrument was used to sequence the amplicons, and resulted reads were processed Geneious 2023.2.1 software to quantify the editing percentages. .. Mammalian cell culture conditions HEK293T cells with a constitutive Cbh Cas9 expression cassette at the AAVS1 locus (GeneCopoeia, SL502) was used for human cell editing experiments. .. Samples (3 μg total protein per lane) were separated by SDS–PAGE on 4%–15% gradient polyacrylamide gels and transferred to PVDF membranes (0.45 μm, Amersham) using a semi-dry blotting system (TE77PWR, Amersham Biosciences) at 2 mA/cm 2 for 50 min. Membranes were pre-activated in methanol and then blocked for 1 h in 2% blocking reagent (ECL, Amersham) in TBS-T buffer (20 mM Tris–HCl, pH 8.0, 180 mM NaCl, 0.1% Tween-20).

    Article Title: Distinct properties and activation of hexameric and heptameric Pannexin 1 channel concatemers
    Article Snippet: .. HEK293T cells stably expressing CRISPR/Cas9 nuclease (co-expressing copGFP and puromycin resistant gene and integrated at the human AAVS1 Safe Harbor Locus) were obtained from GeneCopoeia (Cat# SL502). ..

    Cell Culture:

    Article Title: Structurally related phage helicases trigger type III-A3 retron-mediated anti-phage defense across diverse tailed phage families.
    Article Snippet: An Illumina NextSeq instrument was used to sequence the amplicons, and resulted reads were processed Geneious 2023.2.1 software to quantify the editing percentages. .. Mammalian cell culture conditions HEK293T cells with a constitutive Cbh Cas9 expression cassette at the AAVS1 locus (GeneCopoeia, SL502) was used for human cell editing experiments. .. Samples (3 μg total protein per lane) were separated by SDS–PAGE on 4%–15% gradient polyacrylamide gels and transferred to PVDF membranes (0.45 μm, Amersham) using a semi-dry blotting system (TE77PWR, Amersham Biosciences) at 2 mA/cm 2 for 50 min. Membranes were pre-activated in methanol and then blocked for 1 h in 2% blocking reagent (ECL, Amersham) in TBS-T buffer (20 mM Tris–HCl, pH 8.0, 180 mM NaCl, 0.1% Tween-20).

    Generated:

    Article Title: Multiple cAMP/PKA complexes at the STIM1 ER/PM junction specified by E-Syt1 and E-Syt2 reciprocally gates ANO1 (TMEM16A) via Ca 2+
    Article Snippet: .. IRBIT −/− cell line 3 was generated by transfecting the Cas9 stable cell line from Genecopoeia (SL502) with three different gRNA for human IRBIT gene in HEK293T cells. .. The set of 3 gRNA plasmids pCRISPR-SG01 expressing the DNA sequences for gRNA for IRBIT were obtained from GeneCopoeia (cat #HCP291015-SG01-3).

    Article Title: Multiple cAMP/PKA complexes at the STIM1 ER/PM junction specified by E-Syt1 and E-Syt2 reciprocally gates ANO1 (TMEM16A) via Ca 2 .
    Article Snippet: .. Generating IRBIT knockout (IRBIT−/−) cells IRBIT−/− cell line 3 was generated by transfecting the Cas9 stable cell line from Genecopoeia (SL502) with three different gRNA for human IRBIT gene in HEK293T cells. .. The set of 3 gRNA plasmids pCRISPR-SG01 expressing the DNA sequences for gRNA for IRBIT were obtained from GeneCopoeia (cat #HCP291015-SG01-3).

    Stable Transfection:

    Article Title: Multiple cAMP/PKA complexes at the STIM1 ER/PM junction specified by E-Syt1 and E-Syt2 reciprocally gates ANO1 (TMEM16A) via Ca 2+
    Article Snippet: .. IRBIT −/− cell line 3 was generated by transfecting the Cas9 stable cell line from Genecopoeia (SL502) with three different gRNA for human IRBIT gene in HEK293T cells. .. The set of 3 gRNA plasmids pCRISPR-SG01 expressing the DNA sequences for gRNA for IRBIT were obtained from GeneCopoeia (cat #HCP291015-SG01-3).

    Article Title: Distinct properties and activation of hexameric and heptameric Pannexin 1 channel concatemers
    Article Snippet: .. HEK293T cells stably expressing CRISPR/Cas9 nuclease (co-expressing copGFP and puromycin resistant gene and integrated at the human AAVS1 Safe Harbor Locus) were obtained from GeneCopoeia (Cat# SL502). ..

    Article Title: Multiple cAMP/PKA complexes at the STIM1 ER/PM junction specified by E-Syt1 and E-Syt2 reciprocally gates ANO1 (TMEM16A) via Ca 2 .
    Article Snippet: .. Generating IRBIT knockout (IRBIT−/−) cells IRBIT−/− cell line 3 was generated by transfecting the Cas9 stable cell line from Genecopoeia (SL502) with three different gRNA for human IRBIT gene in HEK293T cells. .. The set of 3 gRNA plasmids pCRISPR-SG01 expressing the DNA sequences for gRNA for IRBIT were obtained from GeneCopoeia (cat #HCP291015-SG01-3).

    CRISPR:

    Article Title: Distinct properties and activation of hexameric and heptameric Pannexin 1 channel concatemers
    Article Snippet: .. HEK293T cells stably expressing CRISPR/Cas9 nuclease (co-expressing copGFP and puromycin resistant gene and integrated at the human AAVS1 Safe Harbor Locus) were obtained from GeneCopoeia (Cat# SL502). ..

    Knock-Out:

    Article Title: Multiple cAMP/PKA complexes at the STIM1 ER/PM junction specified by E-Syt1 and E-Syt2 reciprocally gates ANO1 (TMEM16A) via Ca 2 .
    Article Snippet: .. Generating IRBIT knockout (IRBIT−/−) cells IRBIT−/− cell line 3 was generated by transfecting the Cas9 stable cell line from Genecopoeia (SL502) with three different gRNA for human IRBIT gene in HEK293T cells. .. The set of 3 gRNA plasmids pCRISPR-SG01 expressing the DNA sequences for gRNA for IRBIT were obtained from GeneCopoeia (cat #HCP291015-SG01-3).



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