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control empty vector pegfp c1  (Addgene inc)


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    Addgene inc control empty vector pegfp c1
    Control Empty Vector Pegfp C1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 224 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egfp+empty+vector+control/pEGFP(C1)-RepoMan+(Plasmid+%2344212)/pmc06953137-68-9-18
    Average 95 stars, based on 224 article reviews
    control empty vector pegfp c1 - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Transfection:

    Article Title: Specific proteolysis mediated by a p97-directed proteolysis-targeting chimera (p97-PROTAC)
    Article Snippet: .. Cells were transiently transfected with the following vectors: pcDNA5 FRT/TO GFP-ETV1, pcDNA5 FRT/TO GFP-Emerin, pEYFP-Coilin, pcDNA FRT/TO GFP, VCP(wt)-EGFP (Addgene, #23971), pEGFP-C1-tagged plasmids containing the exon 1 of HTT with 23 CAG repeats (pEGFP-Q23: wild-type HTT, Addgene, #40261) or 74 CAG repeats (pEGFP-Q74: mutant HTT, Addgene, #40262) and pEGFP-Q24 (generously provided by Dr. Maite Castro, Universidad Austral de Chile). ..

    Mutagenesis:

    Article Title: Specific proteolysis mediated by a p97-directed proteolysis-targeting chimera (p97-PROTAC)
    Article Snippet: .. Cells were transiently transfected with the following vectors: pcDNA5 FRT/TO GFP-ETV1, pcDNA5 FRT/TO GFP-Emerin, pEYFP-Coilin, pcDNA FRT/TO GFP, VCP(wt)-EGFP (Addgene, #23971), pEGFP-C1-tagged plasmids containing the exon 1 of HTT with 23 CAG repeats (pEGFP-Q23: wild-type HTT, Addgene, #40261) or 74 CAG repeats (pEGFP-Q74: mutant HTT, Addgene, #40262) and pEGFP-Q24 (generously provided by Dr. Maite Castro, Universidad Austral de Chile). ..

    other:

    Article Title: Nek family members regulate Rad54 during homologous recombination in developing mice
    Article Snippet: H&E images were captured using an Axioimager M1 microscope with ApoTome.2 (Zeiss, Germany) and Metafer4 (MetaSystems) software at 10x and 40x magnification and arranged with Fiji.

    Article Title: Nek family members regulate Rad54 during homologous recombination in developing mice
    Article Snippet: Correct ligation was confirmed with a control digest of the pEGFP constructs with XhoI and NotI-HF (New England Biolabs, USA) and sequencing with primers available at Microsynth AG (EGFPC-for, SV40-pArev).

    Recombinant:

    Article Title: Establishing synthetic ribbon-type active zones in a heterologous expression system
    Article Snippet: Recombinant DNA reagent , pCS2 + -CMV-Bassoon (plasmid) , This study , Addgene plasmid # 250160, RRID: Addgene 250160 , pCS2 + backbone, CMV promoter, rat Bassoon, Kanamycin resistance, SV40 poly(A). .. Recombinant DNA reagent , C1-CMV-palm-Bassoon(95–3938)-EGFP (plasmid) , This study , Addgene plasmid # 250161, RRID: Addgene_ 250161 , pEGFP-C1 backbone, CMV enhancer, palm-rat Bassoon (95–3938)-EGFP, Kanamycin resistance, SV40 poly(A). .. Recombinant DNA reagent , C1-CMV-palm-Bassoon(95–3938)-STOP (plasmid) , This study , Addgene plasmid # 250162, RRID: Addgene_ 250162 , pEGFP-C1 backbone, CMV enhancer, palm-rat Bassoon (95–3938)-STOP, Kanamycin resistance, SV40 poly(A).

    Article Title: Establishing synthetic ribbon-type active zones in a heterologous expression system
    Article Snippet: Recombinant DNA reagent , C1-CMV-palm-Bassoon(95–3938)-EGFP (plasmid) , This study , Addgene plasmid # 250161, RRID: Addgene_ 250161 , pEGFP-C1 backbone, CMV enhancer, palm-rat Bassoon (95–3938)-EGFP, Kanamycin resistance, SV40 poly(A). .. Recombinant DNA reagent , C1-CMV-palm-Bassoon(95–3938)-STOP (plasmid) , This study , Addgene plasmid # 250162, RRID: Addgene_ 250162 , pEGFP-C1 backbone, CMV enhancer, palm-rat Bassoon (95–3938)-STOP, Kanamycin resistance, SV40 poly(A). .. Recombinant DNA reagent , pHTN-CMV-EGFP-Ca V 1.3 (plasmid) , , – , pHTN backbone, CMV enhancer-promoter, EGFP-human CACNA1D, Ampicillin resistance, SV40 poly(A).

    Plasmid Preparation:

    Article Title: Establishing synthetic ribbon-type active zones in a heterologous expression system
    Article Snippet: Recombinant DNA reagent , pCS2 + -CMV-Bassoon (plasmid) , This study , Addgene plasmid # 250160, RRID: Addgene 250160 , pCS2 + backbone, CMV promoter, rat Bassoon, Kanamycin resistance, SV40 poly(A). .. Recombinant DNA reagent , C1-CMV-palm-Bassoon(95–3938)-EGFP (plasmid) , This study , Addgene plasmid # 250161, RRID: Addgene_ 250161 , pEGFP-C1 backbone, CMV enhancer, palm-rat Bassoon (95–3938)-EGFP, Kanamycin resistance, SV40 poly(A). .. Recombinant DNA reagent , C1-CMV-palm-Bassoon(95–3938)-STOP (plasmid) , This study , Addgene plasmid # 250162, RRID: Addgene_ 250162 , pEGFP-C1 backbone, CMV enhancer, palm-rat Bassoon (95–3938)-STOP, Kanamycin resistance, SV40 poly(A).

    Article Title: Establishing synthetic ribbon-type active zones in a heterologous expression system
    Article Snippet: Recombinant DNA reagent , C1-CMV-palm-Bassoon(95–3938)-EGFP (plasmid) , This study , Addgene plasmid # 250161, RRID: Addgene_ 250161 , pEGFP-C1 backbone, CMV enhancer, palm-rat Bassoon (95–3938)-EGFP, Kanamycin resistance, SV40 poly(A). .. Recombinant DNA reagent , C1-CMV-palm-Bassoon(95–3938)-STOP (plasmid) , This study , Addgene plasmid # 250162, RRID: Addgene_ 250162 , pEGFP-C1 backbone, CMV enhancer, palm-rat Bassoon (95–3938)-STOP, Kanamycin resistance, SV40 poly(A). .. Recombinant DNA reagent , pHTN-CMV-EGFP-Ca V 1.3 (plasmid) , , – , pHTN backbone, CMV enhancer-promoter, EGFP-human CACNA1D, Ampicillin resistance, SV40 poly(A).



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    Image Search Results


    a, HCT116 cells stably expressing BRIP1 R162Q were transiently transfected using PEI with empty vector (EV-EGFP) or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.

    Journal: bioRxiv

    Article Title: Role of a childhood cancer-linked BRIP1/FANCJ germline variant in genomic instability and cancer cell vulnerability

    doi: 10.64898/2026.03.24.714005

    Figure Lengend Snippet: a, HCT116 cells stably expressing BRIP1 R162Q were transiently transfected using PEI with empty vector (EV-EGFP) or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.

    Article Snippet: For R-loop removal, cells were transiently transfected with an EGFP empty vector control (pEGFP-C1, Takara Bio, #632470), EGFP-tagged RNaseH1 wild-type (WT), or the catalytically inactive RNaseH1 D145N mutant as indicated.

    Techniques: Stable Transfection, Expressing, Transfection, Plasmid Preparation, Fluorescence, Western Blot, Construct, Immunofluorescence, Labeling, Confocal Microscopy, Two Tailed Test