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control empty vectors plv-egfp  (Genechem)

 
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    Genechem control empty vectors plv-egfp
    Control Empty Vectors Plv Egfp, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egfp+empty+vector+control/control+vectors+plv+egfp/pm28432001-44-14-35
    Average 90 stars, based on 1 article reviews
    control empty vectors plv-egfp - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Recombinant:

    Article Title: Apoptosis repressor with caspase recruitment domain enhances survival and promotes osteogenic differentiation of human osteoblast cells under Zoledronate treatment
    Article Snippet: Recombinant virus plasmid pLV-nol3-enhanced green fluorescent protein (EGFP) and control vectors pLV-EGFP, together with packaging plasmids (pLP1, pLP2 and pLP/VSVG), were co-transfected into HEK293T cells using LipofectamineTM 2000 (all from Genechem Co., Ltd., Shanghai, China).

    Article Title: In vivo and in vitro study of osteogenic potency of endothelin-1 on bone marrow-derived mesenchymal stem cells.
    Article Snippet: Bone marrow-derived mesenchymal stem cells (BMSCs) are a major source of osteoblasts and are crucial for bone remolding and repair and thus they are widely used for tissue engineering applications.. Tissue engineering in combination with gene therapy is considered as a promising approach in new bone regeneration.. Endothelin-1 (EDN-1)is produced by vascular endothelial cells which plays an important role during bone development.

    Virus:

    Article Title: Apoptosis repressor with caspase recruitment domain enhances survival and promotes osteogenic differentiation of human osteoblast cells under Zoledronate treatment
    Article Snippet: Recombinant virus plasmid pLV-nol3-enhanced green fluorescent protein (EGFP) and control vectors pLV-EGFP, together with packaging plasmids (pLP1, pLP2 and pLP/VSVG), were co-transfected into HEK293T cells using LipofectamineTM 2000 (all from Genechem Co., Ltd., Shanghai, China).

    Article Title: In vivo and in vitro study of osteogenic potency of endothelin-1 on bone marrow-derived mesenchymal stem cells.
    Article Snippet: Bone marrow-derived mesenchymal stem cells (BMSCs) are a major source of osteoblasts and are crucial for bone remolding and repair and thus they are widely used for tissue engineering applications.. Tissue engineering in combination with gene therapy is considered as a promising approach in new bone regeneration.. Endothelin-1 (EDN-1)is produced by vascular endothelial cells which plays an important role during bone development.

    Plasmid Preparation:

    Article Title: Apoptosis repressor with caspase recruitment domain enhances survival and promotes osteogenic differentiation of human osteoblast cells under Zoledronate treatment
    Article Snippet: Recombinant virus plasmid pLV-nol3-enhanced green fluorescent protein (EGFP) and control vectors pLV-EGFP, together with packaging plasmids (pLP1, pLP2 and pLP/VSVG), were co-transfected into HEK293T cells using LipofectamineTM 2000 (all from Genechem Co., Ltd., Shanghai, China).

    Article Title: In vivo and in vitro study of osteogenic potency of endothelin-1 on bone marrow-derived mesenchymal stem cells.
    Article Snippet: Bone marrow-derived mesenchymal stem cells (BMSCs) are a major source of osteoblasts and are crucial for bone remolding and repair and thus they are widely used for tissue engineering applications.. Tissue engineering in combination with gene therapy is considered as a promising approach in new bone regeneration.. Endothelin-1 (EDN-1)is produced by vascular endothelial cells which plays an important role during bone development.

    Control:

    Article Title: Apoptosis repressor with caspase recruitment domain enhances survival and promotes osteogenic differentiation of human osteoblast cells under Zoledronate treatment
    Article Snippet: Recombinant virus plasmid pLV-nol3-enhanced green fluorescent protein (EGFP) and control vectors pLV-EGFP, together with packaging plasmids (pLP1, pLP2 and pLP/VSVG), were co-transfected into HEK293T cells using LipofectamineTM 2000 (all from Genechem Co., Ltd., Shanghai, China).

    Article Title: In vivo and in vitro study of osteogenic potency of endothelin-1 on bone marrow-derived mesenchymal stem cells.
    Article Snippet: Bone marrow-derived mesenchymal stem cells (BMSCs) are a major source of osteoblasts and are crucial for bone remolding and repair and thus they are widely used for tissue engineering applications.. Tissue engineering in combination with gene therapy is considered as a promising approach in new bone regeneration.. Endothelin-1 (EDN-1)is produced by vascular endothelial cells which plays an important role during bone development.



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    Image Search Results


    a, HCT116 cells stably expressing BRIP1 R162Q were transiently transfected using PEI with empty vector (EV-EGFP) or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.

    Journal: bioRxiv

    Article Title: Role of a childhood cancer-linked BRIP1/FANCJ germline variant in genomic instability and cancer cell vulnerability

    doi: 10.64898/2026.03.24.714005

    Figure Lengend Snippet: a, HCT116 cells stably expressing BRIP1 R162Q were transiently transfected using PEI with empty vector (EV-EGFP) or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.

    Article Snippet: For R-loop removal, cells were transiently transfected with an EGFP empty vector control (pEGFP-C1, Takara Bio, #632470), EGFP-tagged RNaseH1 wild-type (WT), or the catalytically inactive RNaseH1 D145N mutant as indicated.

    Techniques: Stable Transfection, Expressing, Transfection, Plasmid Preparation, Fluorescence, Western Blot, Construct, Immunofluorescence, Labeling, Confocal Microscopy, Two Tailed Test