effectene (Promega)
90
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Promega
effectene
Effectene, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/effectene/effectene/pm14675151-60-32-23
Average 90 stars, based on 1 article reviews
Effectene, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/effectene/effectene/pm14675151-60-32-23
Average 90 stars, based on 1 article reviews
effectene - by Bioz Stars,
2026-11
90/100 stars
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Transfection:Article Title: C-terminal α Domain of p63 Binds to p300 to Coactivate β-Catenin Article Snippet: .. A total of 130 ng of DNA was transfected into each Article Title: Epistatic Rescue of Nkx2.5 Adult Cardiac Conduction Disease Phenotypes by Prospero-Related Homeobox Protein 1 and HDAC3 Article Snippet: .. NIH3T3 and H9c2 cell culture was carried out under standard conditions and transfections were carried out using Article Title: Link between steroidogenesis, the cell cycle, and PKA in adrenocortical tumor cells. Article Snippet: .. For 161 cell-cycle release of the H295R cells, following 24 or 48 h of synchronization, cells were 162 washed three times with PBS and cultured in medium without drugs for 6, 12, 24, 36, or 48 h. 163 For PKA activation or inhibition, cells were transiently transfected with an siRNA for 164 8 PRKAR1A, the control scramble RNA (siS), PKACA (in the PCDNA3+ vector, a gift from Dr 165 D. 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For transfection of the individual reporters we used Article Title: Salt-inducible kinases regulate growth through the Hippo signalling pathway in Drosophila Article Snippet: .. S2 and S2R+ cells were transfected with indicated plasmids using the Article Title: Curcumin ameliorates renal failure in 5/6 nephrectomized rats: role of inflammation. Article Snippet: doi: 10.1152/ajprenal.90732.2008 296:F1146-F1157, 2009.. First published 18 February 2009;Am J Physiol Renal Physiol T. W. B. Gehr S. S. Ghosh, H. D. Massey, R. Krieg, Z.. A. Fazelbhoy, S. Ghosh, D. A. Sica, I. Fakhry and nephrectomized rats: role of inflammation Curcumin ameliorates renal failure in 5/6 You might find this additional info useful... 64 articles, 24 of which you can access for free at: This article cites http://ajprenal.physiology.org/content/296/5/F1146.full#ref-list-1 6 other HighWire-hosted articles: This article has been cited by http://ajprenal.physiology.org/content/296/5/F1146#cited-by including high resolution figures, can be found at: Updated information and services http://ajprenal.physiology.org/content/296/5/F1146.full found at: can beAmerican Journal of Physiology - Renal Physiology about Additional material and information http://www.the-aps.org/publications/ajprenal This information is current as of January 18, 2013. Cell Culture:Article Title: Epistatic Rescue of Nkx2.5 Adult Cardiac Conduction Disease Phenotypes by Prospero-Related Homeobox Protein 1 and HDAC3 Article Snippet: .. NIH3T3 and H9c2 cell culture was carried out under standard conditions and transfections were carried out using Article Title: Link between steroidogenesis, the cell cycle, and PKA in adrenocortical tumor cells. Article Snippet: .. For 161 cell-cycle release of the H295R cells, following 24 or 48 h of synchronization, cells were 162 washed three times with PBS and cultured in medium without drugs for 6, 12, 24, 36, or 48 h. 163 For PKA activation or inhibition, cells were transiently transfected with an siRNA for 164 8 PRKAR1A, the control scramble RNA (siS), PKACA (in the PCDNA3+ vector, a gift from Dr 165 D. Calebiro), or the empty vector, or incubated with the PKA inhibitor PKI 14-22 166 (Calbiochem, Fontenay sous-Bois France ) and cultured with the different drugs for 24 h. 167 2.6 Transfection and co-transfection 168 H295R cells cultured in six-well plates were transfected with 100 pmol siRNA PRKAR1A 169 (UGAAUGGGCAACCAGUGUUdTdT), the siRNA negative control (siS) 170 (CAGUCGCGUUUGCGACUGGdTdT), 500 ng PKACA or PCDNA3+, as empty vector, 171 using the Activation Assay:Article Title: Link between steroidogenesis, the cell cycle, and PKA in adrenocortical tumor cells. Article Snippet: .. For 161 cell-cycle release of the H295R cells, following 24 or 48 h of synchronization, cells were 162 washed three times with PBS and cultured in medium without drugs for 6, 12, 24, 36, or 48 h. 163 For PKA activation or inhibition, cells were transiently transfected with an siRNA for 164 8 PRKAR1A, the control scramble RNA (siS), PKACA (in the PCDNA3+ vector, a gift from Dr 165 D. Calebiro), or the empty vector, or incubated with the PKA inhibitor PKI 14-22 166 (Calbiochem, Fontenay sous-Bois France ) and cultured with the different drugs for 24 h. 167 2.6 Transfection and co-transfection 168 H295R cells cultured in six-well plates were transfected with 100 pmol siRNA PRKAR1A 169 (UGAAUGGGCAACCAGUGUUdTdT), the siRNA negative control (siS) 170 (CAGUCGCGUUUGCGACUGGdTdT), 500 ng PKACA or PCDNA3+, as empty vector, 171 using the Inhibition:Article Title: Link between steroidogenesis, the cell cycle, and PKA in adrenocortical tumor cells. Article Snippet: .. For 161 cell-cycle release of the H295R cells, following 24 or 48 h of synchronization, cells were 162 washed three times with PBS and cultured in medium without drugs for 6, 12, 24, 36, or 48 h. 163 For PKA activation or inhibition, cells were transiently transfected with an siRNA for 164 8 PRKAR1A, the control scramble RNA (siS), PKACA (in the PCDNA3+ vector, a gift from Dr 165 D. Calebiro), or the empty vector, or incubated with the PKA inhibitor PKI 14-22 166 (Calbiochem, Fontenay sous-Bois France ) and cultured with the different drugs for 24 h. 167 2.6 Transfection and co-transfection 168 H295R cells cultured in six-well plates were transfected with 100 pmol siRNA PRKAR1A 169 (UGAAUGGGCAACCAGUGUUdTdT), the siRNA negative control (siS) 170 (CAGUCGCGUUUGCGACUGGdTdT), 500 ng PKACA or PCDNA3+, as empty vector, 171 using the Control:Article Title: Link between steroidogenesis, the cell cycle, and PKA in adrenocortical tumor cells. Article Snippet: .. For 161 cell-cycle release of the H295R cells, following 24 or 48 h of synchronization, cells were 162 washed three times with PBS and cultured in medium without drugs for 6, 12, 24, 36, or 48 h. 163 For PKA activation or inhibition, cells were transiently transfected with an siRNA for 164 8 PRKAR1A, the control scramble RNA (siS), PKACA (in the PCDNA3+ vector, a gift from Dr 165 D. Calebiro), or the empty vector, or incubated with the PKA inhibitor PKI 14-22 166 (Calbiochem, Fontenay sous-Bois France ) and cultured with the different drugs for 24 h. 167 2.6 Transfection and co-transfection 168 H295R cells cultured in six-well plates were transfected with 100 pmol siRNA PRKAR1A 169 (UGAAUGGGCAACCAGUGUUdTdT), the siRNA negative control (siS) 170 (CAGUCGCGUUUGCGACUGGdTdT), 500 ng PKACA or PCDNA3+, as empty vector, 171 using the Plasmid Preparation:Article Title: Link between steroidogenesis, the cell cycle, and PKA in adrenocortical tumor cells. Article Snippet: .. For 161 cell-cycle release of the H295R cells, following 24 or 48 h of synchronization, cells were 162 washed three times with PBS and cultured in medium without drugs for 6, 12, 24, 36, or 48 h. 163 For PKA activation or inhibition, cells were transiently transfected with an siRNA for 164 8 PRKAR1A, the control scramble RNA (siS), PKACA (in the PCDNA3+ vector, a gift from Dr 165 D. Calebiro), or the empty vector, or incubated with the PKA inhibitor PKI 14-22 166 (Calbiochem, Fontenay sous-Bois France ) and cultured with the different drugs for 24 h. 167 2.6 Transfection and co-transfection 168 H295R cells cultured in six-well plates were transfected with 100 pmol siRNA PRKAR1A 169 (UGAAUGGGCAACCAGUGUUdTdT), the siRNA negative control (siS) 170 (CAGUCGCGUUUGCGACUGGdTdT), 500 ng PKACA or PCDNA3+, as empty vector, 171 using the Incubation:Article Title: Link between steroidogenesis, the cell cycle, and PKA in adrenocortical tumor cells. Article Snippet: .. For 161 cell-cycle release of the H295R cells, following 24 or 48 h of synchronization, cells were 162 washed three times with PBS and cultured in medium without drugs for 6, 12, 24, 36, or 48 h. 163 For PKA activation or inhibition, cells were transiently transfected with an siRNA for 164 8 PRKAR1A, the control scramble RNA (siS), PKACA (in the PCDNA3+ vector, a gift from Dr 165 D. Calebiro), or the empty vector, or incubated with the PKA inhibitor PKI 14-22 166 (Calbiochem, Fontenay sous-Bois France ) and cultured with the different drugs for 24 h. 167 2.6 Transfection and co-transfection 168 H295R cells cultured in six-well plates were transfected with 100 pmol siRNA PRKAR1A 169 (UGAAUGGGCAACCAGUGUUdTdT), the siRNA negative control (siS) 170 (CAGUCGCGUUUGCGACUGGdTdT), 500 ng PKACA or PCDNA3+, as empty vector, 171 using the Negative Control:Article Title: Link between steroidogenesis, the cell cycle, and PKA in adrenocortical tumor cells. Article Snippet: .. For 161 cell-cycle release of the H295R cells, following 24 or 48 h of synchronization, cells were 162 washed three times with PBS and cultured in medium without drugs for 6, 12, 24, 36, or 48 h. 163 For PKA activation or inhibition, cells were transiently transfected with an siRNA for 164 8 PRKAR1A, the control scramble RNA (siS), PKACA (in the PCDNA3+ vector, a gift from Dr 165 D. Calebiro), or the empty vector, or incubated with the PKA inhibitor PKI 14-22 166 (Calbiochem, Fontenay sous-Bois France ) and cultured with the different drugs for 24 h. 167 2.6 Transfection and co-transfection 168 H295R cells cultured in six-well plates were transfected with 100 pmol siRNA PRKAR1A 169 (UGAAUGGGCAACCAGUGUUdTdT), the siRNA negative control (siS) 170 (CAGUCGCGUUUGCGACUGGdTdT), 500 ng PKACA or PCDNA3+, as empty vector, 171 using the other:Article Title: Nerfin-1 represses transcriptional output of Hippo signaling in cell competition Article Snippet: ON-TARGET plus siRNA SMARTpools were purchased from Dharmacon. |