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lentiviral overexpression vectors pcslenti ef1 puro cmvaldh1b1 wpre  (Obio Technology Corp Ltd)

 
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    Obio Technology Corp Ltd lentiviral overexpression vectors pcslenti ef1 puro cmvaldh1b1 wpre
    Lentiviral Overexpression Vectors Pcslenti Ef1 Puro Cmvaldh1b1 Wpre, supplied by Obio Technology Corp Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ef1/0+dio+ef1%CE%B1+enphr3+eyfp+pa+raav+wpre/pm42284509-252-1-15
    Average 86 stars, based on 1 article reviews
    lentiviral overexpression vectors pcslenti ef1 puro cmvaldh1b1 wpre - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Over Expression:

    Article Title: BIN1 and ALDH1B1 Deficiency in Colonic Smooth Muscle Drives Mitochondrial Dysfunction and Fibrosis in Slow-Transit Constipation.
    Article Snippet: .. The lentiviral overexpression vectors pcSLenti-EF1-Puro-CMVALDH1B1-WPRE (ALDH1B1- OE) and pcSLenti-EF1-Puro- CMVBIN1-WPRE (BIN1-OE) were obtained from Shanghai Obio Technology (Group) Corp., Ltd. (Shanghai, China). ..

    Article Title: LincRNA-EPS alleviates osteoclastogenesis under inflammatory microenvironment through preventing excessive iron metabolism
    Article Snippet: .. Following lentivirus were purchased from OBiO Technology (Shanghai) Corp., Ltd: lincRNA-EPS overexpression lentivirus (pASLenti-pA-Ttc39aos1-CMV-EF1-EGFP-WPRE) and negative control (pASLenti-pA-MCS-CMV-EF1-EGFP-WPRE), Lcn2 knockdown lentivirus (pCLenti-U6-shRNA(Lcn2)-CMV-EGFP-WPRE) and negative control (pCLenti-U6-shRNA(NC)-CMV-EGFP-WPRE). ..

    Article Title: NBPF1 acts as a tumor suppressor in prostate cancer by regulating the PI3K/AKT pathway
    Article Snippet: Knockdown was performed using specific short hairpin RNAs (shRNAs) against NBPF1, which were commercially sourced from Tsingke Biotech (Guangzhou, China). .. Conversely, for overexpression, the NBPF1 coding sequence was cloned into the pcSLenti-CMV-MCS-3xFLAG-Puro-WPRE lentiviral construct, purchased from Obio Technology (Shanghai, China). ..

    Virus:

    Article Title: miRNA-378a-5p attenuates the development of abdominal aortic aneurysm via ABLIM1-MKL1 signaling pathways
    Article Snippet: Additionally, antagomir-378a-5p and antagomir-NC were purchased from Guangzhou RiboBio Co., Ltd. 6-week-old ApoE −/− mice were injected with antagomir-378a-5p and antagomir-NC via the tail vein 3 times per week for 4 weeks (50 nmol each time). .. Adeno-associated virus serotype 2/9 (AAV2/9) carrying the Ablim1 coding sequence (CDS) with a Sm22α promoter (AAV-SM22- Ablim1 ) or the control virus (AAV-SM22-NC) were constructed by OBiO Technology Corp., Ltd. 6-week-old ApoE −/− mice were injected with AAV-SM22- Ablim1 or the control virus (AAV-SM22-NC) via the tail vein at a dosage of 5×10 11 vg per mouse. ..

    Article Title: Targeted AAV6 gene therapy restores corneal endothelial function in three hereditary corneal dystrophies.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit ZO-1 antibody Abcam Cat#ab221547; RRID: AB_2892660 Alexa Fluor 594-conjugated donkey anti-rabbit IgG Abcam Cat#ab150076; RRID: AB_2782993 Goat Anti-Chst5 Antibody Absolute Biotech Cat#EB09182; RRID: AB_2244836 Rabbit WNT5A/B Polyclonal Antibody Proteintech Cat#55184-1-AP; RRID: AB_2881285 Rabbit CD45 antibody Abcam Cat#ab10558; RRID: AB_442810 Alexa Fluor 594-conjugated donkey anti-goat IgG Abcam Cat#ab150132; RRID: AB_2810222 β-actin antibody Abcam Cat#ab179467; RRID: AB_2737344 APC anti-mouse CD45 antibody BioLegend Cat#103111; RRID: AB_312976 APC anti-mouse CD4 antibody BioLegend Cat#100411; RRID: AB_312696 APC anti-mouse Ly6g antibody BioLegend Cat#164505; RRID: AB_2927993 APC Rat IgG2a, κ Isotype Ctrl Antibody BioLegend Cat#400512; RRID: AB_2814702 APC Rat IgG2b, κ Isotype Ctrl Antibody BioLegend Cat#400611; RRID: AB_326555 Bacterial and virus strains pAAV2-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV8-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV9-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-Chst5-3×FLAG-P2A-EGFP-tWPA OBiO Technology N/A pAAV6-CMV-Col8a2-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-Slc4a11-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-Wnt5a-3×FLAG-P2A-EGFP-WPRE OBiO Technology N/A pAAV6-U6-shWnt5a-CMV-EGFP-WPRE OBiO Technology N/A Biological samples MCD patient cornea samples Eye Bank of Qingdao Eye Hospital N/A Human donor cornea samples Eye Bank of Qingdao Eye Hospital N/A Chemicals, peptides, and recombinant proteins 4% Paraformaldehyde Fixative Solution Biosharp Cat#BL539A DAPI solution Solarbio Cat#C0065 Bright-Lite Luciferase Assay Vazyme Cat#DD1204-04 Alizarin red S solution Solarbio Cat#G1083 TransZol Up Plus RNA Kit TransGen Biotech Cat#ER501-01 PrimeScriptTM RT Reagent Kit Takara Cat#RR037A RIPA lysis buffer Solarbio Cat#P0010 PMSF Solarbio Cat#P0100 BCA Protein Assay Kit Solarbio Cat#PC0020 Alcian Blue staining solution Solarbio Cat#G1560 Dispase II Roche Cat#44390400 Trypsin Gibco Cat#25200-056 Collagenase A Roche Cat#48655301 10× Genomics Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 Pleasanton Cat#1000268 Thapsigargin Sigma-Aldrich Cat#T9033 (Continued on next page) Cell Reports Medicine 7, 102649, March 17, 2026 e1 .. C57BL/6J mice, aged 6–8 weeks, were procured from Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China).

    Article Title: miRNA-378a-5p attenuates the development of abdominal aortic aneurysm via ABLIM1-MKL1 signaling pathways
    Article Snippet: .. To achieve VSMCs-specific knockdown of Ablim1 , adeno-associated virus serotype 2/9 (AAV2/9) carrying mouse sh Ablim1 sequence with a Sm22α promoter (AAV-SM22-sh Ablim1 ) or the control virus (AAV-SM22-shNC) were constructed by OBiO Technology Corp., Ltd. 6-week-old ApoE −/− mice were injected with AAV-SM22-sh Ablim1 or the control virus (AAV-SM22-NC) via the tail vein at a dosage of 5×10 11 vg per mouse. ..

    Sequencing:

    Article Title: miRNA-378a-5p attenuates the development of abdominal aortic aneurysm via ABLIM1-MKL1 signaling pathways
    Article Snippet: Additionally, antagomir-378a-5p and antagomir-NC were purchased from Guangzhou RiboBio Co., Ltd. 6-week-old ApoE −/− mice were injected with antagomir-378a-5p and antagomir-NC via the tail vein 3 times per week for 4 weeks (50 nmol each time). .. Adeno-associated virus serotype 2/9 (AAV2/9) carrying the Ablim1 coding sequence (CDS) with a Sm22α promoter (AAV-SM22- Ablim1 ) or the control virus (AAV-SM22-NC) were constructed by OBiO Technology Corp., Ltd. 6-week-old ApoE −/− mice were injected with AAV-SM22- Ablim1 or the control virus (AAV-SM22-NC) via the tail vein at a dosage of 5×10 11 vg per mouse. ..

    Article Title: miRNA-378a-5p attenuates the development of abdominal aortic aneurysm via ABLIM1-MKL1 signaling pathways
    Article Snippet: .. To achieve VSMCs-specific knockdown of Ablim1 , adeno-associated virus serotype 2/9 (AAV2/9) carrying mouse sh Ablim1 sequence with a Sm22α promoter (AAV-SM22-sh Ablim1 ) or the control virus (AAV-SM22-shNC) were constructed by OBiO Technology Corp., Ltd. 6-week-old ApoE −/− mice were injected with AAV-SM22-sh Ablim1 or the control virus (AAV-SM22-NC) via the tail vein at a dosage of 5×10 11 vg per mouse. ..

    Article Title: NBPF1 acts as a tumor suppressor in prostate cancer by regulating the PI3K/AKT pathway
    Article Snippet: Knockdown was performed using specific short hairpin RNAs (shRNAs) against NBPF1, which were commercially sourced from Tsingke Biotech (Guangzhou, China). .. Conversely, for overexpression, the NBPF1 coding sequence was cloned into the pcSLenti-CMV-MCS-3xFLAG-Puro-WPRE lentiviral construct, purchased from Obio Technology (Shanghai, China). ..

    Bioprocessing:

    Article Title: miRNA-378a-5p attenuates the development of abdominal aortic aneurysm via ABLIM1-MKL1 signaling pathways
    Article Snippet: Additionally, antagomir-378a-5p and antagomir-NC were purchased from Guangzhou RiboBio Co., Ltd. 6-week-old ApoE −/− mice were injected with antagomir-378a-5p and antagomir-NC via the tail vein 3 times per week for 4 weeks (50 nmol each time). .. Adeno-associated virus serotype 2/9 (AAV2/9) carrying the Ablim1 coding sequence (CDS) with a Sm22α promoter (AAV-SM22- Ablim1 ) or the control virus (AAV-SM22-NC) were constructed by OBiO Technology Corp., Ltd. 6-week-old ApoE −/− mice were injected with AAV-SM22- Ablim1 or the control virus (AAV-SM22-NC) via the tail vein at a dosage of 5×10 11 vg per mouse. ..

    Article Title: miRNA-378a-5p attenuates the development of abdominal aortic aneurysm via ABLIM1-MKL1 signaling pathways
    Article Snippet: .. To achieve VSMCs-specific knockdown of Ablim1 , adeno-associated virus serotype 2/9 (AAV2/9) carrying mouse sh Ablim1 sequence with a Sm22α promoter (AAV-SM22-sh Ablim1 ) or the control virus (AAV-SM22-shNC) were constructed by OBiO Technology Corp., Ltd. 6-week-old ApoE −/− mice were injected with AAV-SM22-sh Ablim1 or the control virus (AAV-SM22-NC) via the tail vein at a dosage of 5×10 11 vg per mouse. ..

    Control:

    Article Title: miRNA-378a-5p attenuates the development of abdominal aortic aneurysm via ABLIM1-MKL1 signaling pathways
    Article Snippet: Additionally, antagomir-378a-5p and antagomir-NC were purchased from Guangzhou RiboBio Co., Ltd. 6-week-old ApoE −/− mice were injected with antagomir-378a-5p and antagomir-NC via the tail vein 3 times per week for 4 weeks (50 nmol each time). .. Adeno-associated virus serotype 2/9 (AAV2/9) carrying the Ablim1 coding sequence (CDS) with a Sm22α promoter (AAV-SM22- Ablim1 ) or the control virus (AAV-SM22-NC) were constructed by OBiO Technology Corp., Ltd. 6-week-old ApoE −/− mice were injected with AAV-SM22- Ablim1 or the control virus (AAV-SM22-NC) via the tail vein at a dosage of 5×10 11 vg per mouse. ..

    Article Title: miRNA-378a-5p attenuates the development of abdominal aortic aneurysm via ABLIM1-MKL1 signaling pathways
    Article Snippet: .. To achieve VSMCs-specific knockdown of Ablim1 , adeno-associated virus serotype 2/9 (AAV2/9) carrying mouse sh Ablim1 sequence with a Sm22α promoter (AAV-SM22-sh Ablim1 ) or the control virus (AAV-SM22-shNC) were constructed by OBiO Technology Corp., Ltd. 6-week-old ApoE −/− mice were injected with AAV-SM22-sh Ablim1 or the control virus (AAV-SM22-NC) via the tail vein at a dosage of 5×10 11 vg per mouse. ..

    Construct:

    Article Title: miRNA-378a-5p attenuates the development of abdominal aortic aneurysm via ABLIM1-MKL1 signaling pathways
    Article Snippet: Additionally, antagomir-378a-5p and antagomir-NC were purchased from Guangzhou RiboBio Co., Ltd. 6-week-old ApoE −/− mice were injected with antagomir-378a-5p and antagomir-NC via the tail vein 3 times per week for 4 weeks (50 nmol each time). .. Adeno-associated virus serotype 2/9 (AAV2/9) carrying the Ablim1 coding sequence (CDS) with a Sm22α promoter (AAV-SM22- Ablim1 ) or the control virus (AAV-SM22-NC) were constructed by OBiO Technology Corp., Ltd. 6-week-old ApoE −/− mice were injected with AAV-SM22- Ablim1 or the control virus (AAV-SM22-NC) via the tail vein at a dosage of 5×10 11 vg per mouse. ..

    Article Title: miRNA-378a-5p attenuates the development of abdominal aortic aneurysm via ABLIM1-MKL1 signaling pathways
    Article Snippet: .. To achieve VSMCs-specific knockdown of Ablim1 , adeno-associated virus serotype 2/9 (AAV2/9) carrying mouse sh Ablim1 sequence with a Sm22α promoter (AAV-SM22-sh Ablim1 ) or the control virus (AAV-SM22-shNC) were constructed by OBiO Technology Corp., Ltd. 6-week-old ApoE −/− mice were injected with AAV-SM22-sh Ablim1 or the control virus (AAV-SM22-NC) via the tail vein at a dosage of 5×10 11 vg per mouse. ..

    Article Title: NBPF1 acts as a tumor suppressor in prostate cancer by regulating the PI3K/AKT pathway
    Article Snippet: Knockdown was performed using specific short hairpin RNAs (shRNAs) against NBPF1, which were commercially sourced from Tsingke Biotech (Guangzhou, China). .. Conversely, for overexpression, the NBPF1 coding sequence was cloned into the pcSLenti-CMV-MCS-3xFLAG-Puro-WPRE lentiviral construct, purchased from Obio Technology (Shanghai, China). ..

    Injection:

    Article Title: miRNA-378a-5p attenuates the development of abdominal aortic aneurysm via ABLIM1-MKL1 signaling pathways
    Article Snippet: Additionally, antagomir-378a-5p and antagomir-NC were purchased from Guangzhou RiboBio Co., Ltd. 6-week-old ApoE −/− mice were injected with antagomir-378a-5p and antagomir-NC via the tail vein 3 times per week for 4 weeks (50 nmol each time). .. Adeno-associated virus serotype 2/9 (AAV2/9) carrying the Ablim1 coding sequence (CDS) with a Sm22α promoter (AAV-SM22- Ablim1 ) or the control virus (AAV-SM22-NC) were constructed by OBiO Technology Corp., Ltd. 6-week-old ApoE −/− mice were injected with AAV-SM22- Ablim1 or the control virus (AAV-SM22-NC) via the tail vein at a dosage of 5×10 11 vg per mouse. ..

    Article Title: miRNA-378a-5p attenuates the development of abdominal aortic aneurysm via ABLIM1-MKL1 signaling pathways
    Article Snippet: .. To achieve VSMCs-specific knockdown of Ablim1 , adeno-associated virus serotype 2/9 (AAV2/9) carrying mouse sh Ablim1 sequence with a Sm22α promoter (AAV-SM22-sh Ablim1 ) or the control virus (AAV-SM22-shNC) were constructed by OBiO Technology Corp., Ltd. 6-week-old ApoE −/− mice were injected with AAV-SM22-sh Ablim1 or the control virus (AAV-SM22-NC) via the tail vein at a dosage of 5×10 11 vg per mouse. ..

    Article Title: Hypoactivity, abnormal development of dendrites relevant to impaired synaptic transmission of calretinin-expressing interneurons in the medial prefrontal cortex underlies social deficits of mouse model in autism.
    Article Snippet: .. For optogenetic experiments, pAAV-hSyn-DIO-hChR2(H134R)-EYFP-WPRE (300 nL, titer: 6.63 × 1012 genome copies/mL, from OBiO Technology) was unilaterally injected into the mPFC (AP: 2.0 mm; ML: ± 0.3 mm; DV: -2.0 mm). ..

    Recombinant:

    Article Title: Targeted AAV6 gene therapy restores corneal endothelial function in three hereditary corneal dystrophies.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit ZO-1 antibody Abcam Cat#ab221547; RRID: AB_2892660 Alexa Fluor 594-conjugated donkey anti-rabbit IgG Abcam Cat#ab150076; RRID: AB_2782993 Goat Anti-Chst5 Antibody Absolute Biotech Cat#EB09182; RRID: AB_2244836 Rabbit WNT5A/B Polyclonal Antibody Proteintech Cat#55184-1-AP; RRID: AB_2881285 Rabbit CD45 antibody Abcam Cat#ab10558; RRID: AB_442810 Alexa Fluor 594-conjugated donkey anti-goat IgG Abcam Cat#ab150132; RRID: AB_2810222 β-actin antibody Abcam Cat#ab179467; RRID: AB_2737344 APC anti-mouse CD45 antibody BioLegend Cat#103111; RRID: AB_312976 APC anti-mouse CD4 antibody BioLegend Cat#100411; RRID: AB_312696 APC anti-mouse Ly6g antibody BioLegend Cat#164505; RRID: AB_2927993 APC Rat IgG2a, κ Isotype Ctrl Antibody BioLegend Cat#400512; RRID: AB_2814702 APC Rat IgG2b, κ Isotype Ctrl Antibody BioLegend Cat#400611; RRID: AB_326555 Bacterial and virus strains pAAV2-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV8-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV9-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-Chst5-3×FLAG-P2A-EGFP-tWPA OBiO Technology N/A pAAV6-CMV-Col8a2-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-Slc4a11-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-Wnt5a-3×FLAG-P2A-EGFP-WPRE OBiO Technology N/A pAAV6-U6-shWnt5a-CMV-EGFP-WPRE OBiO Technology N/A Biological samples MCD patient cornea samples Eye Bank of Qingdao Eye Hospital N/A Human donor cornea samples Eye Bank of Qingdao Eye Hospital N/A Chemicals, peptides, and recombinant proteins 4% Paraformaldehyde Fixative Solution Biosharp Cat#BL539A DAPI solution Solarbio Cat#C0065 Bright-Lite Luciferase Assay Vazyme Cat#DD1204-04 Alizarin red S solution Solarbio Cat#G1083 TransZol Up Plus RNA Kit TransGen Biotech Cat#ER501-01 PrimeScriptTM RT Reagent Kit Takara Cat#RR037A RIPA lysis buffer Solarbio Cat#P0010 PMSF Solarbio Cat#P0100 BCA Protein Assay Kit Solarbio Cat#PC0020 Alcian Blue staining solution Solarbio Cat#G1560 Dispase II Roche Cat#44390400 Trypsin Gibco Cat#25200-056 Collagenase A Roche Cat#48655301 10× Genomics Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 Pleasanton Cat#1000268 Thapsigargin Sigma-Aldrich Cat#T9033 (Continued on next page) Cell Reports Medicine 7, 102649, March 17, 2026 e1 .. C57BL/6J mice, aged 6–8 weeks, were procured from Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China).

    Luciferase:

    Article Title: Targeted AAV6 gene therapy restores corneal endothelial function in three hereditary corneal dystrophies.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit ZO-1 antibody Abcam Cat#ab221547; RRID: AB_2892660 Alexa Fluor 594-conjugated donkey anti-rabbit IgG Abcam Cat#ab150076; RRID: AB_2782993 Goat Anti-Chst5 Antibody Absolute Biotech Cat#EB09182; RRID: AB_2244836 Rabbit WNT5A/B Polyclonal Antibody Proteintech Cat#55184-1-AP; RRID: AB_2881285 Rabbit CD45 antibody Abcam Cat#ab10558; RRID: AB_442810 Alexa Fluor 594-conjugated donkey anti-goat IgG Abcam Cat#ab150132; RRID: AB_2810222 β-actin antibody Abcam Cat#ab179467; RRID: AB_2737344 APC anti-mouse CD45 antibody BioLegend Cat#103111; RRID: AB_312976 APC anti-mouse CD4 antibody BioLegend Cat#100411; RRID: AB_312696 APC anti-mouse Ly6g antibody BioLegend Cat#164505; RRID: AB_2927993 APC Rat IgG2a, κ Isotype Ctrl Antibody BioLegend Cat#400512; RRID: AB_2814702 APC Rat IgG2b, κ Isotype Ctrl Antibody BioLegend Cat#400611; RRID: AB_326555 Bacterial and virus strains pAAV2-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV8-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV9-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-Chst5-3×FLAG-P2A-EGFP-tWPA OBiO Technology N/A pAAV6-CMV-Col8a2-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-Slc4a11-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-Wnt5a-3×FLAG-P2A-EGFP-WPRE OBiO Technology N/A pAAV6-U6-shWnt5a-CMV-EGFP-WPRE OBiO Technology N/A Biological samples MCD patient cornea samples Eye Bank of Qingdao Eye Hospital N/A Human donor cornea samples Eye Bank of Qingdao Eye Hospital N/A Chemicals, peptides, and recombinant proteins 4% Paraformaldehyde Fixative Solution Biosharp Cat#BL539A DAPI solution Solarbio Cat#C0065 Bright-Lite Luciferase Assay Vazyme Cat#DD1204-04 Alizarin red S solution Solarbio Cat#G1083 TransZol Up Plus RNA Kit TransGen Biotech Cat#ER501-01 PrimeScriptTM RT Reagent Kit Takara Cat#RR037A RIPA lysis buffer Solarbio Cat#P0010 PMSF Solarbio Cat#P0100 BCA Protein Assay Kit Solarbio Cat#PC0020 Alcian Blue staining solution Solarbio Cat#G1560 Dispase II Roche Cat#44390400 Trypsin Gibco Cat#25200-056 Collagenase A Roche Cat#48655301 10× Genomics Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 Pleasanton Cat#1000268 Thapsigargin Sigma-Aldrich Cat#T9033 (Continued on next page) Cell Reports Medicine 7, 102649, March 17, 2026 e1 .. C57BL/6J mice, aged 6–8 weeks, were procured from Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China).

    Lysis:

    Article Title: Targeted AAV6 gene therapy restores corneal endothelial function in three hereditary corneal dystrophies.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit ZO-1 antibody Abcam Cat#ab221547; RRID: AB_2892660 Alexa Fluor 594-conjugated donkey anti-rabbit IgG Abcam Cat#ab150076; RRID: AB_2782993 Goat Anti-Chst5 Antibody Absolute Biotech Cat#EB09182; RRID: AB_2244836 Rabbit WNT5A/B Polyclonal Antibody Proteintech Cat#55184-1-AP; RRID: AB_2881285 Rabbit CD45 antibody Abcam Cat#ab10558; RRID: AB_442810 Alexa Fluor 594-conjugated donkey anti-goat IgG Abcam Cat#ab150132; RRID: AB_2810222 β-actin antibody Abcam Cat#ab179467; RRID: AB_2737344 APC anti-mouse CD45 antibody BioLegend Cat#103111; RRID: AB_312976 APC anti-mouse CD4 antibody BioLegend Cat#100411; RRID: AB_312696 APC anti-mouse Ly6g antibody BioLegend Cat#164505; RRID: AB_2927993 APC Rat IgG2a, κ Isotype Ctrl Antibody BioLegend Cat#400512; RRID: AB_2814702 APC Rat IgG2b, κ Isotype Ctrl Antibody BioLegend Cat#400611; RRID: AB_326555 Bacterial and virus strains pAAV2-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV8-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV9-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-Chst5-3×FLAG-P2A-EGFP-tWPA OBiO Technology N/A pAAV6-CMV-Col8a2-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-Slc4a11-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-Wnt5a-3×FLAG-P2A-EGFP-WPRE OBiO Technology N/A pAAV6-U6-shWnt5a-CMV-EGFP-WPRE OBiO Technology N/A Biological samples MCD patient cornea samples Eye Bank of Qingdao Eye Hospital N/A Human donor cornea samples Eye Bank of Qingdao Eye Hospital N/A Chemicals, peptides, and recombinant proteins 4% Paraformaldehyde Fixative Solution Biosharp Cat#BL539A DAPI solution Solarbio Cat#C0065 Bright-Lite Luciferase Assay Vazyme Cat#DD1204-04 Alizarin red S solution Solarbio Cat#G1083 TransZol Up Plus RNA Kit TransGen Biotech Cat#ER501-01 PrimeScriptTM RT Reagent Kit Takara Cat#RR037A RIPA lysis buffer Solarbio Cat#P0010 PMSF Solarbio Cat#P0100 BCA Protein Assay Kit Solarbio Cat#PC0020 Alcian Blue staining solution Solarbio Cat#G1560 Dispase II Roche Cat#44390400 Trypsin Gibco Cat#25200-056 Collagenase A Roche Cat#48655301 10× Genomics Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 Pleasanton Cat#1000268 Thapsigargin Sigma-Aldrich Cat#T9033 (Continued on next page) Cell Reports Medicine 7, 102649, March 17, 2026 e1 .. C57BL/6J mice, aged 6–8 weeks, were procured from Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China).

    Bicinchoninic Acid Protein Assay:

    Article Title: Targeted AAV6 gene therapy restores corneal endothelial function in three hereditary corneal dystrophies.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit ZO-1 antibody Abcam Cat#ab221547; RRID: AB_2892660 Alexa Fluor 594-conjugated donkey anti-rabbit IgG Abcam Cat#ab150076; RRID: AB_2782993 Goat Anti-Chst5 Antibody Absolute Biotech Cat#EB09182; RRID: AB_2244836 Rabbit WNT5A/B Polyclonal Antibody Proteintech Cat#55184-1-AP; RRID: AB_2881285 Rabbit CD45 antibody Abcam Cat#ab10558; RRID: AB_442810 Alexa Fluor 594-conjugated donkey anti-goat IgG Abcam Cat#ab150132; RRID: AB_2810222 β-actin antibody Abcam Cat#ab179467; RRID: AB_2737344 APC anti-mouse CD45 antibody BioLegend Cat#103111; RRID: AB_312976 APC anti-mouse CD4 antibody BioLegend Cat#100411; RRID: AB_312696 APC anti-mouse Ly6g antibody BioLegend Cat#164505; RRID: AB_2927993 APC Rat IgG2a, κ Isotype Ctrl Antibody BioLegend Cat#400512; RRID: AB_2814702 APC Rat IgG2b, κ Isotype Ctrl Antibody BioLegend Cat#400611; RRID: AB_326555 Bacterial and virus strains pAAV2-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV8-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV9-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-Chst5-3×FLAG-P2A-EGFP-tWPA OBiO Technology N/A pAAV6-CMV-Col8a2-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-Slc4a11-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-Wnt5a-3×FLAG-P2A-EGFP-WPRE OBiO Technology N/A pAAV6-U6-shWnt5a-CMV-EGFP-WPRE OBiO Technology N/A Biological samples MCD patient cornea samples Eye Bank of Qingdao Eye Hospital N/A Human donor cornea samples Eye Bank of Qingdao Eye Hospital N/A Chemicals, peptides, and recombinant proteins 4% Paraformaldehyde Fixative Solution Biosharp Cat#BL539A DAPI solution Solarbio Cat#C0065 Bright-Lite Luciferase Assay Vazyme Cat#DD1204-04 Alizarin red S solution Solarbio Cat#G1083 TransZol Up Plus RNA Kit TransGen Biotech Cat#ER501-01 PrimeScriptTM RT Reagent Kit Takara Cat#RR037A RIPA lysis buffer Solarbio Cat#P0010 PMSF Solarbio Cat#P0100 BCA Protein Assay Kit Solarbio Cat#PC0020 Alcian Blue staining solution Solarbio Cat#G1560 Dispase II Roche Cat#44390400 Trypsin Gibco Cat#25200-056 Collagenase A Roche Cat#48655301 10× Genomics Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 Pleasanton Cat#1000268 Thapsigargin Sigma-Aldrich Cat#T9033 (Continued on next page) Cell Reports Medicine 7, 102649, March 17, 2026 e1 .. C57BL/6J mice, aged 6–8 weeks, were procured from Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China).

    Staining:

    Article Title: Targeted AAV6 gene therapy restores corneal endothelial function in three hereditary corneal dystrophies.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit ZO-1 antibody Abcam Cat#ab221547; RRID: AB_2892660 Alexa Fluor 594-conjugated donkey anti-rabbit IgG Abcam Cat#ab150076; RRID: AB_2782993 Goat Anti-Chst5 Antibody Absolute Biotech Cat#EB09182; RRID: AB_2244836 Rabbit WNT5A/B Polyclonal Antibody Proteintech Cat#55184-1-AP; RRID: AB_2881285 Rabbit CD45 antibody Abcam Cat#ab10558; RRID: AB_442810 Alexa Fluor 594-conjugated donkey anti-goat IgG Abcam Cat#ab150132; RRID: AB_2810222 β-actin antibody Abcam Cat#ab179467; RRID: AB_2737344 APC anti-mouse CD45 antibody BioLegend Cat#103111; RRID: AB_312976 APC anti-mouse CD4 antibody BioLegend Cat#100411; RRID: AB_312696 APC anti-mouse Ly6g antibody BioLegend Cat#164505; RRID: AB_2927993 APC Rat IgG2a, κ Isotype Ctrl Antibody BioLegend Cat#400512; RRID: AB_2814702 APC Rat IgG2b, κ Isotype Ctrl Antibody BioLegend Cat#400611; RRID: AB_326555 Bacterial and virus strains pAAV2-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV8-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV9-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-Chst5-3×FLAG-P2A-EGFP-tWPA OBiO Technology N/A pAAV6-CMV-Col8a2-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-Slc4a11-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-Wnt5a-3×FLAG-P2A-EGFP-WPRE OBiO Technology N/A pAAV6-U6-shWnt5a-CMV-EGFP-WPRE OBiO Technology N/A Biological samples MCD patient cornea samples Eye Bank of Qingdao Eye Hospital N/A Human donor cornea samples Eye Bank of Qingdao Eye Hospital N/A Chemicals, peptides, and recombinant proteins 4% Paraformaldehyde Fixative Solution Biosharp Cat#BL539A DAPI solution Solarbio Cat#C0065 Bright-Lite Luciferase Assay Vazyme Cat#DD1204-04 Alizarin red S solution Solarbio Cat#G1083 TransZol Up Plus RNA Kit TransGen Biotech Cat#ER501-01 PrimeScriptTM RT Reagent Kit Takara Cat#RR037A RIPA lysis buffer Solarbio Cat#P0010 PMSF Solarbio Cat#P0100 BCA Protein Assay Kit Solarbio Cat#PC0020 Alcian Blue staining solution Solarbio Cat#G1560 Dispase II Roche Cat#44390400 Trypsin Gibco Cat#25200-056 Collagenase A Roche Cat#48655301 10× Genomics Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 Pleasanton Cat#1000268 Thapsigargin Sigma-Aldrich Cat#T9033 (Continued on next page) Cell Reports Medicine 7, 102649, March 17, 2026 e1 .. C57BL/6J mice, aged 6–8 weeks, were procured from Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China).

    Single Cell:

    Article Title: Targeted AAV6 gene therapy restores corneal endothelial function in three hereditary corneal dystrophies.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit ZO-1 antibody Abcam Cat#ab221547; RRID: AB_2892660 Alexa Fluor 594-conjugated donkey anti-rabbit IgG Abcam Cat#ab150076; RRID: AB_2782993 Goat Anti-Chst5 Antibody Absolute Biotech Cat#EB09182; RRID: AB_2244836 Rabbit WNT5A/B Polyclonal Antibody Proteintech Cat#55184-1-AP; RRID: AB_2881285 Rabbit CD45 antibody Abcam Cat#ab10558; RRID: AB_442810 Alexa Fluor 594-conjugated donkey anti-goat IgG Abcam Cat#ab150132; RRID: AB_2810222 β-actin antibody Abcam Cat#ab179467; RRID: AB_2737344 APC anti-mouse CD45 antibody BioLegend Cat#103111; RRID: AB_312976 APC anti-mouse CD4 antibody BioLegend Cat#100411; RRID: AB_312696 APC anti-mouse Ly6g antibody BioLegend Cat#164505; RRID: AB_2927993 APC Rat IgG2a, κ Isotype Ctrl Antibody BioLegend Cat#400512; RRID: AB_2814702 APC Rat IgG2b, κ Isotype Ctrl Antibody BioLegend Cat#400611; RRID: AB_326555 Bacterial and virus strains pAAV2-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV8-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV9-CMV-EGFP-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-Chst5-3×FLAG-P2A-EGFP-tWPA OBiO Technology N/A pAAV6-CMV-Col8a2-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-Slc4a11-3×FLAG-tWPA OBiO Technology N/A pAAV6-CMV-Wnt5a-3×FLAG-P2A-EGFP-WPRE OBiO Technology N/A pAAV6-U6-shWnt5a-CMV-EGFP-WPRE OBiO Technology N/A Biological samples MCD patient cornea samples Eye Bank of Qingdao Eye Hospital N/A Human donor cornea samples Eye Bank of Qingdao Eye Hospital N/A Chemicals, peptides, and recombinant proteins 4% Paraformaldehyde Fixative Solution Biosharp Cat#BL539A DAPI solution Solarbio Cat#C0065 Bright-Lite Luciferase Assay Vazyme Cat#DD1204-04 Alizarin red S solution Solarbio Cat#G1083 TransZol Up Plus RNA Kit TransGen Biotech Cat#ER501-01 PrimeScriptTM RT Reagent Kit Takara Cat#RR037A RIPA lysis buffer Solarbio Cat#P0010 PMSF Solarbio Cat#P0100 BCA Protein Assay Kit Solarbio Cat#PC0020 Alcian Blue staining solution Solarbio Cat#G1560 Dispase II Roche Cat#44390400 Trypsin Gibco Cat#25200-056 Collagenase A Roche Cat#48655301 10× Genomics Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 Pleasanton Cat#1000268 Thapsigargin Sigma-Aldrich Cat#T9033 (Continued on next page) Cell Reports Medicine 7, 102649, March 17, 2026 e1 .. C57BL/6J mice, aged 6–8 weeks, were procured from Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China).

    Negative Control:

    Article Title: LincRNA-EPS alleviates osteoclastogenesis under inflammatory microenvironment through preventing excessive iron metabolism
    Article Snippet: .. Following lentivirus were purchased from OBiO Technology (Shanghai) Corp., Ltd: lincRNA-EPS overexpression lentivirus (pASLenti-pA-Ttc39aos1-CMV-EF1-EGFP-WPRE) and negative control (pASLenti-pA-MCS-CMV-EF1-EGFP-WPRE), Lcn2 knockdown lentivirus (pCLenti-U6-shRNA(Lcn2)-CMV-EGFP-WPRE) and negative control (pCLenti-U6-shRNA(NC)-CMV-EGFP-WPRE). ..

    Knockdown:

    Article Title: LincRNA-EPS alleviates osteoclastogenesis under inflammatory microenvironment through preventing excessive iron metabolism
    Article Snippet: .. Following lentivirus were purchased from OBiO Technology (Shanghai) Corp., Ltd: lincRNA-EPS overexpression lentivirus (pASLenti-pA-Ttc39aos1-CMV-EF1-EGFP-WPRE) and negative control (pASLenti-pA-MCS-CMV-EF1-EGFP-WPRE), Lcn2 knockdown lentivirus (pCLenti-U6-shRNA(Lcn2)-CMV-EGFP-WPRE) and negative control (pCLenti-U6-shRNA(NC)-CMV-EGFP-WPRE). ..

    Article Title: miRNA-378a-5p attenuates the development of abdominal aortic aneurysm via ABLIM1-MKL1 signaling pathways
    Article Snippet: .. To achieve VSMCs-specific knockdown of Ablim1 , adeno-associated virus serotype 2/9 (AAV2/9) carrying mouse sh Ablim1 sequence with a Sm22α promoter (AAV-SM22-sh Ablim1 ) or the control virus (AAV-SM22-shNC) were constructed by OBiO Technology Corp., Ltd. 6-week-old ApoE −/− mice were injected with AAV-SM22-sh Ablim1 or the control virus (AAV-SM22-NC) via the tail vein at a dosage of 5×10 11 vg per mouse. ..

    Clone Assay:

    Article Title: NBPF1 acts as a tumor suppressor in prostate cancer by regulating the PI3K/AKT pathway
    Article Snippet: Knockdown was performed using specific short hairpin RNAs (shRNAs) against NBPF1, which were commercially sourced from Tsingke Biotech (Guangzhou, China). .. Conversely, for overexpression, the NBPF1 coding sequence was cloned into the pcSLenti-CMV-MCS-3xFLAG-Puro-WPRE lentiviral construct, purchased from Obio Technology (Shanghai, China). ..

    Plasmid Preparation:

    Article Title: MDK Activates the PI3K/AKT Axis to Induce AP2A1 Expression and Epithelial–Mesenchymal Transition in Colorectal Cancer
    Article Snippet: MDK shRNAs (pLV[shRNA]-EGFP:T2A:Puro-U6 > hMDK) were obtained from VectorBuilder (Guangzhou, Guangdong, China). .. The plasmid pSLenti-EF1-EGFP-P2A-Puro-CMV-MDK-3xFLAG-WPRE was acquired from OBiO Technology (Shanghai) Corp., Ltd., Shanghai, China. ..



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