tris edta sucrose buffer (Thermo Fisher)

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Thermo Fisher tris edta sucrose buffer
Tris Edta Sucrose Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/edta+buffer/TES%2C+0%2E2M+buffer+soln%2E%2C+pH+7%2E5/pm42237848-62-8-28
Average 94 stars, based on 1 article reviews

tris edta sucrose buffer - by Bioz Stars, 2026-10

94/100 stars

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Preserving:

Article Title: Simulated Oxygen Supply Efficiency Assessment to Represent Stored Red Blood Cells Quality
Article Snippet: .. The following reagents and instruments were used in this study: bovine whole blood (Created Biotechnology, Beijing, China), sodium hydroxide (NaOH; Sinopharm, Shanghai, China), potassium chloride (KCl; Sinopharm, China), TES buffer (Thermo Fisher, Waltham, MA, USA), bovine serum albumin (BSA; Thermo Fisher, Waltham, MA, USA), Dimethylpolysiloxane (Thermo Fisher, Waltham, MA, USA), citrate-phosphate-dextrose-adenine-1 (CPDA-1) cell preservation buffer (Thermo Fisher, USA), leukoreduction filter (Bengbu Medical College, Bengbu, China), BLOODOX-2018 oxygen binding-dissociation analyzer ( Softron Biotechnology, Beijing, China), and ӒKTA pure chromatography system (GE Health, Marlborough, MA, USA). ..

Binding Assay:

Chromatography:

Purification:

Article Title: Single nucleotide polymorphism analysis of a Trichoderma reesei hyper-cellulolytic mutant developed in Japan.
Article Snippet: .. About 100mg of mycelia was added to a pre-warmed solution of TES (10mM Tris–HCl pH 7.5, 10mM EDTA, and 0.5% SDS), containing watersaturated phenol and zirconium beads, and vortexed at maximum speed for 15min at 65 C. The supernatant was extracted and purified with TRIzol LS reagent (Invitrogen, Carlsbad, CA, USA). .. After centrifugation, the supernatant was loaded onto an RNA Spin Mini column (GE Healthcare), and the RNA was further purified following the supplier’s instructions.

Incubation:

Article Title: Generation of human sequence antibodies in OmniChicken against the highly conserved mammalian target BDNF using three distinct discovery workflows.
Article Snippet: .. Following overnight incubation, periplasmic extract was prepared in Tris-EDTA-sucrose buffer (TES; 50 mM Tris-HCl, 1 mM ethylenediaminetetraacetic acid (EDTA), 20% Sucrose, pH 8) and blocked with 1% Casein/PBS (Thermo Scientific) to obtain a final concentration of 50% blocked periplasmic extract. .. Ninety-six well NUNC Maxisorp plates were pre-coated overnight at 4°C with 100 μL BDNF (Sino Biologics Inc, 50,240) at a concentration of 5 μg/mL in PBS pH 7.4.

Concentration Assay:

Nucleic Acid Electrophoresis:

Article Title: SecDF as Part of the Sec-Translocase Facilitates Efficient Secretion of Bacillus cereus Toxins and Cell Wall-Associated Proteins
Article Snippet: .. For gel electrophoresis, 8 ml of duplicate, independent and normalized supernatants of cultures grown in LBG were concentrated 40-fold by methanol:acetone precipitation, resuspended in 250 μl TES (20 mM Tris pH 7.5, 0.8% NaCl, 1 mM EDTA), and 12 μl of each sample was analyzed on a 4–20% SDS-polyacrylamide gel (Pierce) by silver staining (Sigma-Aldrich). ..

Silver Staining:

Centrifugation:

Article Title: Topoisomerases regulate alternative transcription start site selection in yeast
Article Snippet: .. Per 10 OD units, RNA was extracted with Tris-EDTA-SDS (TES) buffer (10 mM Tris-HCl, pH 7.5, 10 mM EDTA, 0.5% SDS) and Acid Phenol:Chloroform:Isoamyl alcohol (125:24:1, Ambion) at 65 °C for 45 min. After centrifugation at 4 °C, max speed, 10 min, the aqueous phase was transferred to cold ethanol with 0.3 M sodium acetate. ..

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