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human fgfr2 protein  (Carna Inc)


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    Structured Review

    Carna Inc human fgfr2 protein
    Human Fgfr2 Protein, supplied by Carna Inc, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ect1/FGFR2/us09108973-803-3-13
    Average 94 stars, based on 12 article reviews
    human fgfr2 protein - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: Pyrimidine derivative, method for preparing same and use thereof in medicine
    Article Snippet: .. Recombinant human FGFR protein was purchased from Carna bioscience (Japan, Cat. No: FGFR1 #08-133, FGFR2 #08-134, FGFR3 #08-135, and FGFR4 #08-136). ..

    Activity Assay:

    Article Title: 2 H -pyrazolo[3,4- d ]pyrimidin-4-amine derivatives as novel selective fibroblast growth factor receptor 2 (FGFR2) inhibitors.
    Article Snippet: .. FGFR kinase activity inhibition assay PLW559 was preincubated with FGFR1, FGFR2, FGFR3 or FGFR4 kinase (carna Biosciences) and substrate peptide (Peptide30, Gl) in 50 mmol/l hePes, ph 7.5, 10 mmol/l Mgcl2, 1 mmol/l eGta, 0.01% Brij-35, 2 mmol/l Dtt, and 0.05% Bsa for 30 min at room temperature. compounds were transferred 200 nl of each well from the 384-well echo plate to a 384-well assay plate in duplicates by echo. atP was added to a total concentration of 100 μmol/l and incubated for a period of time, and reactions were stopped using 100 mmol/l hePes, ph 7.5, 50 mmol/l eDta, 0.015% Brij-35 and 0.2% coating reagent #3 (Perkinelmer). the substrate conversion ratio was calculated for each PlW559 concentration and was then used to calculate ic50 values. ..

    Inhibition:

    Article Title: 2 H -pyrazolo[3,4- d ]pyrimidin-4-amine derivatives as novel selective fibroblast growth factor receptor 2 (FGFR2) inhibitors.
    Article Snippet: .. FGFR kinase activity inhibition assay PLW559 was preincubated with FGFR1, FGFR2, FGFR3 or FGFR4 kinase (carna Biosciences) and substrate peptide (Peptide30, Gl) in 50 mmol/l hePes, ph 7.5, 10 mmol/l Mgcl2, 1 mmol/l eGta, 0.01% Brij-35, 2 mmol/l Dtt, and 0.05% Bsa for 30 min at room temperature. compounds were transferred 200 nl of each well from the 384-well echo plate to a 384-well assay plate in duplicates by echo. atP was added to a total concentration of 100 μmol/l and incubated for a period of time, and reactions were stopped using 100 mmol/l hePes, ph 7.5, 50 mmol/l eDta, 0.015% Brij-35 and 0.2% coating reagent #3 (Perkinelmer). the substrate conversion ratio was calculated for each PlW559 concentration and was then used to calculate ic50 values. ..

    Concentration Assay:

    Article Title: 2 H -pyrazolo[3,4- d ]pyrimidin-4-amine derivatives as novel selective fibroblast growth factor receptor 2 (FGFR2) inhibitors.
    Article Snippet: .. FGFR kinase activity inhibition assay PLW559 was preincubated with FGFR1, FGFR2, FGFR3 or FGFR4 kinase (carna Biosciences) and substrate peptide (Peptide30, Gl) in 50 mmol/l hePes, ph 7.5, 10 mmol/l Mgcl2, 1 mmol/l eGta, 0.01% Brij-35, 2 mmol/l Dtt, and 0.05% Bsa for 30 min at room temperature. compounds were transferred 200 nl of each well from the 384-well echo plate to a 384-well assay plate in duplicates by echo. atP was added to a total concentration of 100 μmol/l and incubated for a period of time, and reactions were stopped using 100 mmol/l hePes, ph 7.5, 50 mmol/l eDta, 0.015% Brij-35 and 0.2% coating reagent #3 (Perkinelmer). the substrate conversion ratio was calculated for each PlW559 concentration and was then used to calculate ic50 values. ..

    Incubation:

    Article Title: 2 H -pyrazolo[3,4- d ]pyrimidin-4-amine derivatives as novel selective fibroblast growth factor receptor 2 (FGFR2) inhibitors.
    Article Snippet: .. FGFR kinase activity inhibition assay PLW559 was preincubated with FGFR1, FGFR2, FGFR3 or FGFR4 kinase (carna Biosciences) and substrate peptide (Peptide30, Gl) in 50 mmol/l hePes, ph 7.5, 10 mmol/l Mgcl2, 1 mmol/l eGta, 0.01% Brij-35, 2 mmol/l Dtt, and 0.05% Bsa for 30 min at room temperature. compounds were transferred 200 nl of each well from the 384-well echo plate to a 384-well assay plate in duplicates by echo. atP was added to a total concentration of 100 μmol/l and incubated for a period of time, and reactions were stopped using 100 mmol/l hePes, ph 7.5, 50 mmol/l eDta, 0.015% Brij-35 and 0.2% coating reagent #3 (Perkinelmer). the substrate conversion ratio was calculated for each PlW559 concentration and was then used to calculate ic50 values. ..

    Article Title: rlay-10k_20201231.htm
    Article Snippet: The selectivity of RLY-4008 for FGFR2 over FGFR1 was determined by comparing the potency (IC50) of RLY-4008 and other clinical non-selective FGFR inhibitors in biochemical assays using Caliper technology (PerkinElmer). .. Human FGFR1 and FGFR2 (Carna Biosciences) were incubated with a peptide substrate (PerkinElmer) in the presence of varying concentrations of the indicated inhibitor for 30 minutes. ..

    Article Title: Inhibitors of fibroblast growth factor receptor kinases
    Article Snippet: Both substrate and product were measured and the ratio of these values used to generate % conversion of substrate to product by the LabChip EZ reader (Perkin Elmer). .. Wild type FGFR2 (Carna Bioscience, 08-134) at 0.06 nM was prepared with 1.5 μM substrate, 10 mM MgCl and 100 μM ATP in a buffer containing 50 nM HEPES, 1 mM EGTA, 0.01% Brij-35, 0.05% BSA, and 2 mM DTT prior to addition of compounds in DMSO and incubation for 80 minutes at room temperature. ..

    Article Title: Discovery of KIN-3248, An Irreversible, Next Generation FGFR Inhibitor for the Treatment of Advanced Tumors Harboring FGFR2 and/or FGFR3 Gene Alterations.
    Article Snippet: Fibroblast growth factor receptor (FGFR) alterations are present as oncogenic drivers and bypass mechanisms in many forms of cancer.. These alterations can include fusions, amplifications, rearrangements, and mutations.. Acquired drug resistance to current FGFR inhibitors often results in disease progression and unfavorable outcomes for patients.

    Mutagenesis:

    Article Title: A Model for Decoding Resistance in Precision Oncology: Acquired Resistance to FGFR inhibitors in Cholangiocarcinoma.
    Article Snippet: .. FGFR2 wild type and mutant cellular IC50 values were determined by Carna Biosciences, Inc. using the NanoBRET Target Engagement Assay. ..

    Drug discovery:

    Article Title: A Model for Decoding Resistance in Precision Oncology: Acquired Resistance to FGFR inhibitors in Cholangiocarcinoma.
    Article Snippet: .. FGFR2 wild type and mutant cellular IC50 values were determined by Carna Biosciences, Inc. using the NanoBRET Target Engagement Assay. ..



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    In vitro validation of key genes and associated ligand signaling involved in the response to chemoradiotherapy in cervical cancer. (A) mRNA expression of CAMP in normal cervical squamous epithelial cells from the external cervical os <t>(Ect1/E6E7)</t> and the cervical cancer cell line HeLa following chemoradiotherapy (RCT) treatment. RCT was simulated by X-ray irradiation at graded doses (Gy) for 30 min, followed by cisplatin (DDP) treatment for 24 h. (B–I) mRNA expression levels of differentially regulated ligand signals in HeLa cells from the Blank, CAMP overexpression (CAMP-OE), CAMP knockdown (CAMP-KD), RCT, RCT + CAMP-OE, and RCT + CAMP-KD groups. (J,K) mRNA expression levels of key ligand–receptor pairs in neutrophils and HeLa cells after co-culture before and after RCT treatment. Expression of the ligands COL1A1 (J) , COL1A2 (K) , and ANXA1 (L) was measured in HeLa cells, whereas expression of the receptors CD44 (M) , FPR1 (N) , and FPR2 (O) was assessed in neutrophils.
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    The inhibition of L. crispatus -derived D-LA on FOXD1 expression and growth of Ect1/E6E7 cells. A . immunofluorescence staining for HPV16 E7 protein in Ect1/E6E7 cells after 24-hour treatment with L. crispatus or L. gasseri culture supernatants, or D-LA. Nuclei are counterstained with DAPI (blue). Scale bar = 50 μm. B . HPV16 fluorescence intensity after treatments (mean±SD, n = 3; ** p < 0.01). C . CCK-8 assay measuring cell vitality after treatments (mean±SD, n = 3; ** p < 0.01). D . qPCR analysis of FOXD1 mRNA levels relative to GAPDH (mean±SD, n = 3; * p < 0.05, ** p < 0.01). Four co-culture system: without additives (control), with L. crispatus culture supernatant ( L. crispatus group), with L. gasseri culture supernatant ( L. gasseri group), or with purified D-LA (D-LA group)

    Journal: Journal of Translational Medicine

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus -derived D-lactic acid with cervical gene expression and precancerous cervical lesions

    doi: 10.1186/s12967-026-07982-w

    Figure Lengend Snippet: The inhibition of L. crispatus -derived D-LA on FOXD1 expression and growth of Ect1/E6E7 cells. A . immunofluorescence staining for HPV16 E7 protein in Ect1/E6E7 cells after 24-hour treatment with L. crispatus or L. gasseri culture supernatants, or D-LA. Nuclei are counterstained with DAPI (blue). Scale bar = 50 μm. B . HPV16 fluorescence intensity after treatments (mean±SD, n = 3; ** p < 0.01). C . CCK-8 assay measuring cell vitality after treatments (mean±SD, n = 3; ** p < 0.01). D . qPCR analysis of FOXD1 mRNA levels relative to GAPDH (mean±SD, n = 3; * p < 0.05, ** p < 0.01). Four co-culture system: without additives (control), with L. crispatus culture supernatant ( L. crispatus group), with L. gasseri culture supernatant ( L. gasseri group), or with purified D-LA (D-LA group)

    Article Snippet: The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).

    Techniques: Inhibition, Derivative Assay, Expressing, Immunofluorescence, Staining, Fluorescence, CCK-8 Assay, Co-Culture Assay, Control, Purification

    In vitro validation of key genes and associated ligand signaling involved in the response to chemoradiotherapy in cervical cancer. (A) mRNA expression of CAMP in normal cervical squamous epithelial cells from the external cervical os (Ect1/E6E7) and the cervical cancer cell line HeLa following chemoradiotherapy (RCT) treatment. RCT was simulated by X-ray irradiation at graded doses (Gy) for 30 min, followed by cisplatin (DDP) treatment for 24 h. (B–I) mRNA expression levels of differentially regulated ligand signals in HeLa cells from the Blank, CAMP overexpression (CAMP-OE), CAMP knockdown (CAMP-KD), RCT, RCT + CAMP-OE, and RCT + CAMP-KD groups. (J,K) mRNA expression levels of key ligand–receptor pairs in neutrophils and HeLa cells after co-culture before and after RCT treatment. Expression of the ligands COL1A1 (J) , COL1A2 (K) , and ANXA1 (L) was measured in HeLa cells, whereas expression of the receptors CD44 (M) , FPR1 (N) , and FPR2 (O) was assessed in neutrophils.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Single-cell and multi-omics analyses identify CAMP-associated neutrophil remodeling during radiochemotherapy in cervical cancer

    doi: 10.3389/fcell.2026.1773562

    Figure Lengend Snippet: In vitro validation of key genes and associated ligand signaling involved in the response to chemoradiotherapy in cervical cancer. (A) mRNA expression of CAMP in normal cervical squamous epithelial cells from the external cervical os (Ect1/E6E7) and the cervical cancer cell line HeLa following chemoradiotherapy (RCT) treatment. RCT was simulated by X-ray irradiation at graded doses (Gy) for 30 min, followed by cisplatin (DDP) treatment for 24 h. (B–I) mRNA expression levels of differentially regulated ligand signals in HeLa cells from the Blank, CAMP overexpression (CAMP-OE), CAMP knockdown (CAMP-KD), RCT, RCT + CAMP-OE, and RCT + CAMP-KD groups. (J,K) mRNA expression levels of key ligand–receptor pairs in neutrophils and HeLa cells after co-culture before and after RCT treatment. Expression of the ligands COL1A1 (J) , COL1A2 (K) , and ANXA1 (L) was measured in HeLa cells, whereas expression of the receptors CD44 (M) , FPR1 (N) , and FPR2 (O) was assessed in neutrophils.

    Article Snippet: HeLa and Ect1 cell lines were obtained from ATCC.

    Techniques: In Vitro, Biomarker Discovery, Expressing, Irradiation, Over Expression, Knockdown, Co-Culture Assay