ect1/e6e7 (CHI Scientific Inc)
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Ect1/E6e7, supplied by CHI Scientific Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ect1/ect1%2Fe6e7/pmc07791384-69-0-7
Average 90 stars, based on 1 article reviews
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1) Product Images from "CXCL5 contributes to the tumorigenicity of cervical cancer and is post-transcriptionally regulated by miR-577"
Article Title: CXCL5 contributes to the tumorigenicity of cervical cancer and is post-transcriptionally regulated by miR-577
Journal: International Journal of Clinical and Experimental Pathology
doi:
Figure Legend Snippet: CXCL5 is up-regulated in cervical cancer tissues and cell lines. IHC staining was performed to analyze CXCL5 positive staining in cervical cancer tissues and para-carcinoma tissues (A: left: magnification 40×; right: magnification 200×) and quantitative analysis (B). Western blot was used to measure CXCL5 protein expression in human normal cervical squamous cell line Ect1/E6E7 and cervical cancer cell lines Hela and SiHa (C and D). *P < 0.05. CC, cervical cancer.
Techniques Used: Immunohistochemistry, Staining, Western Blot, Expressing
Related Articles
Transfection:Article Title: CXCL5 contributes to the tumorigenicity of cervical cancer and is post-transcriptionally regulated by miR-577 Article Snippet: Human normal cervical squamous cell line Ect1/E6E7 (CHI Scientific, Inc., Maynard, MA, USA) and cervical cancer cell lines Hela and SiHa (ATCC, USA) were maintained in DMEM; (Gibco, USA) containing 10% FBS (Gibco, USA) with 5% CO 2 at 37°C. miR-Con, miR-577 mimics, miR-577-Mut, and overexpressed CXCL5 plasmids were purchased from GenePharm (Shanghai, China) and transfected into Hela and SiHa cells using lipofectamine 2000 (Invitrogen). Immunohistochemistry:Article Title: CXCL5 contributes to the tumorigenicity of cervical cancer and is post-transcriptionally regulated by miR-577 Article Snippet: Human normal cervical squamous cell line Ect1/E6E7 (CHI Scientific, Inc., Maynard, MA, USA) and cervical cancer cell lines Hela and SiHa (ATCC, USA) were maintained in DMEM; (Gibco, USA) containing 10% FBS (Gibco, USA) with 5% CO 2 at 37°C. miR-Con, miR-577 mimics, miR-577-Mut, and overexpressed CXCL5 plasmids were purchased from GenePharm (Shanghai, China) and transfected into Hela and SiHa cells using lipofectamine 2000 (Invitrogen). Staining:Article Title: CXCL5 contributes to the tumorigenicity of cervical cancer and is post-transcriptionally regulated by miR-577 Article Snippet: Human normal cervical squamous cell line Ect1/E6E7 (CHI Scientific, Inc., Maynard, MA, USA) and cervical cancer cell lines Hela and SiHa (ATCC, USA) were maintained in DMEM; (Gibco, USA) containing 10% FBS (Gibco, USA) with 5% CO 2 at 37°C. miR-Con, miR-577 mimics, miR-577-Mut, and overexpressed CXCL5 plasmids were purchased from GenePharm (Shanghai, China) and transfected into Hela and SiHa cells using lipofectamine 2000 (Invitrogen). Western Blot:Article Title: CXCL5 contributes to the tumorigenicity of cervical cancer and is post-transcriptionally regulated by miR-577 Article Snippet: Human normal cervical squamous cell line Ect1/E6E7 (CHI Scientific, Inc., Maynard, MA, USA) and cervical cancer cell lines Hela and SiHa (ATCC, USA) were maintained in DMEM; (Gibco, USA) containing 10% FBS (Gibco, USA) with 5% CO 2 at 37°C. miR-Con, miR-577 mimics, miR-577-Mut, and overexpressed CXCL5 plasmids were purchased from GenePharm (Shanghai, China) and transfected into Hela and SiHa cells using lipofectamine 2000 (Invitrogen). Expressing:Article Title: CXCL5 contributes to the tumorigenicity of cervical cancer and is post-transcriptionally regulated by miR-577 Article Snippet: Human normal cervical squamous cell line Ect1/E6E7 (CHI Scientific, Inc., Maynard, MA, USA) and cervical cancer cell lines Hela and SiHa (ATCC, USA) were maintained in DMEM; (Gibco, USA) containing 10% FBS (Gibco, USA) with 5% CO 2 at 37°C. miR-Con, miR-577 mimics, miR-577-Mut, and overexpressed CXCL5 plasmids were purchased from GenePharm (Shanghai, China) and transfected into Hela and SiHa cells using lipofectamine 2000 (Invitrogen). |

