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anti fgfr2  (Proteintech)


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    Structured Review

    Proteintech anti fgfr2
    Anti Fgfr2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ect1/FGFR2+Antibody/pm41672244-87-28-36
    Average 93 stars, based on 22 article reviews
    anti fgfr2 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    other:

    Article Title: FGF22/FGFR2/YAP modulates ferroptosis to suppress neurodegeneration and cognitive impairment in Alzheimer's disease.
    Article Snippet: Ferroptosis, a programmed cell death triggered by iron accumulation and lipid peroxidation, has been increasingly recognized as a critical mechanism underlying neurodegenerative processes, including Alzheimer's disease (AD).. The mechanosensitive regulator YAP is implicated in AD progression and ferroptosis.. Here we confirmed that FGF22, a fibroblast growth factor, amelitorated cognitive deficits in β-Amyloid (1–42) (Aβ1–42) treated AD model mice through the FGFR2/YAP pathway, which was further ascertained by various biochemical analyses.

    Article Title: Targeting the FGF1/FGFR signaling pathway enhances the antitumor effect of oxaliplatin and alleviates chemotherapy-induced peripheral neuropathy.
    Article Snippet: Antibodies and conjugates were obtained from the following sources: rabbit monoclonal anti-FGF1 (PA5-79249), anti-p-FGFR (PA5-105938), and anti-p-FRS2 (PA5-118578) from Thermo Fisher Scientific; rabbit anti-FRS2 (11503-1- AP), anti-FGFR1 (60325-1-Ig), anti-FGFR2 (84205-4-RR), anti-FGFR3 (66954-1-Ig), and anti-FGFR4 (81069-1-RR) from ProteinTech Group; rabbit anti-p-PLCγ (M00677), anti-PLCγ (BM4477), anti-p-STAT3 (BM4835), anti-STAT3 (BM4052), anti-p-AKT (BM4721), anti-AKT (A00024), anti-p-ERK1/2 (BM3950), and anti-ERK1/2 (BM3966) from Boster Group; and mouse anti-β-actin (sc-47778) from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Staining:

    Article Title: Using Cancer-Associated Fibroblasts as a Shear-Wave Elastography Imaging Biomarker to Predict Anti-PD-1 Efficacy of Triple-Negative Breast Cancer.
    Article Snippet: .. The primary antibodies used for IF staining include VTN antibody (Proteintech, Wuhan, China, 66398-1-lg), FGFR2 antibody (Proteintech, Wuhan, China, 13042-1-AP), CD8a antibody (Proteintech, Wuhan, China, 29896-1-AP), and SHMT1 antibody (Proteintech, Wuhan, China, 67963-1-Ig). ..

    Blocking Assay:

    Article Title: Rejuvenation of THY1 + nucleus pulposus-derived stem cells promotes intervertebral disc regeneration through FGF10-FGFR1-CREB pathway and mitochondrial fission.
    Article Snippet: Equal amounts of protein from each sample were separated by SDS-PAGE on 8%, 10%, or 12.5% SDS-polyacrylamide gels and then transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore, USA). .. After blocking with 5% skim milk for 60 min at room temperature, the PVDF membranes were incubated overnight at 4°C with rabbit primary antibodies against FGF10 (1:1000, Abcam, UK), P16INK4A (1:1000, Proteintech, USA), P21 (1:1000, Proteintech, USA), GAPDH (1:5000, Proteintech, USA), FGFR1 (1:1000, Abcam, UK), pFGFR1 (1:1000, Abcam, UK), FGFR2 (1:1000, Affinity, USA), FGFR3 (1:1000, Affinity, USA), FGFR4 (1:1000, Affinity, USA), ARG2 (1:1000, Proteintech, USA), DRP1 (1:1000, Proteintech, USA), OPA1 (1:1000, Proteintech , USA), CREB (1:1000, HUABIO, China), pCREB (1:1000, HUABIO, China). ..

    Incubation:

    Article Title: Rejuvenation of THY1 + nucleus pulposus-derived stem cells promotes intervertebral disc regeneration through FGF10-FGFR1-CREB pathway and mitochondrial fission.
    Article Snippet: Equal amounts of protein from each sample were separated by SDS-PAGE on 8%, 10%, or 12.5% SDS-polyacrylamide gels and then transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore, USA). .. After blocking with 5% skim milk for 60 min at room temperature, the PVDF membranes were incubated overnight at 4°C with rabbit primary antibodies against FGF10 (1:1000, Abcam, UK), P16INK4A (1:1000, Proteintech, USA), P21 (1:1000, Proteintech, USA), GAPDH (1:5000, Proteintech, USA), FGFR1 (1:1000, Abcam, UK), pFGFR1 (1:1000, Abcam, UK), FGFR2 (1:1000, Affinity, USA), FGFR3 (1:1000, Affinity, USA), FGFR4 (1:1000, Affinity, USA), ARG2 (1:1000, Proteintech, USA), DRP1 (1:1000, Proteintech, USA), OPA1 (1:1000, Proteintech , USA), CREB (1:1000, HUABIO, China), pCREB (1:1000, HUABIO, China). ..



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    The inhibition of L. crispatus -derived D-LA on FOXD1 expression and growth of Ect1/E6E7 cells. A . immunofluorescence staining for HPV16 E7 protein in Ect1/E6E7 cells after 24-hour treatment with L. crispatus or L. gasseri culture supernatants, or D-LA. Nuclei are counterstained with DAPI (blue). Scale bar = 50 μm. B . HPV16 fluorescence intensity after treatments (mean±SD, n = 3; ** p < 0.01). C . CCK-8 assay measuring cell vitality after treatments (mean±SD, n = 3; ** p < 0.01). D . qPCR analysis of FOXD1 mRNA levels relative to GAPDH (mean±SD, n = 3; * p < 0.05, ** p < 0.01). Four co-culture system: without additives (control), with L. crispatus culture supernatant ( L. crispatus group), with L. gasseri culture supernatant ( L. gasseri group), or with purified D-LA (D-LA group)

    Journal: Journal of Translational Medicine

    Article Title: Integrated cross-sectional study and functional validation indicate the association of lactobacillus crispatus -derived D-lactic acid with cervical gene expression and precancerous cervical lesions

    doi: 10.1186/s12967-026-07982-w

    Figure Lengend Snippet: The inhibition of L. crispatus -derived D-LA on FOXD1 expression and growth of Ect1/E6E7 cells. A . immunofluorescence staining for HPV16 E7 protein in Ect1/E6E7 cells after 24-hour treatment with L. crispatus or L. gasseri culture supernatants, or D-LA. Nuclei are counterstained with DAPI (blue). Scale bar = 50 μm. B . HPV16 fluorescence intensity after treatments (mean±SD, n = 3; ** p < 0.01). C . CCK-8 assay measuring cell vitality after treatments (mean±SD, n = 3; ** p < 0.01). D . qPCR analysis of FOXD1 mRNA levels relative to GAPDH (mean±SD, n = 3; * p < 0.05, ** p < 0.01). Four co-culture system: without additives (control), with L. crispatus culture supernatant ( L. crispatus group), with L. gasseri culture supernatant ( L. gasseri group), or with purified D-LA (D-LA group)

    Article Snippet: The human immortalized cervical squamous cell line Ect1/E6E7 was purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).

    Techniques: Inhibition, Derivative Assay, Expressing, Immunofluorescence, Staining, Fluorescence, CCK-8 Assay, Co-Culture Assay, Control, Purification

    In vitro validation of key genes and associated ligand signaling involved in the response to chemoradiotherapy in cervical cancer. (A) mRNA expression of CAMP in normal cervical squamous epithelial cells from the external cervical os (Ect1/E6E7) and the cervical cancer cell line HeLa following chemoradiotherapy (RCT) treatment. RCT was simulated by X-ray irradiation at graded doses (Gy) for 30 min, followed by cisplatin (DDP) treatment for 24 h. (B–I) mRNA expression levels of differentially regulated ligand signals in HeLa cells from the Blank, CAMP overexpression (CAMP-OE), CAMP knockdown (CAMP-KD), RCT, RCT + CAMP-OE, and RCT + CAMP-KD groups. (J,K) mRNA expression levels of key ligand–receptor pairs in neutrophils and HeLa cells after co-culture before and after RCT treatment. Expression of the ligands COL1A1 (J) , COL1A2 (K) , and ANXA1 (L) was measured in HeLa cells, whereas expression of the receptors CD44 (M) , FPR1 (N) , and FPR2 (O) was assessed in neutrophils.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Single-cell and multi-omics analyses identify CAMP-associated neutrophil remodeling during radiochemotherapy in cervical cancer

    doi: 10.3389/fcell.2026.1773562

    Figure Lengend Snippet: In vitro validation of key genes and associated ligand signaling involved in the response to chemoradiotherapy in cervical cancer. (A) mRNA expression of CAMP in normal cervical squamous epithelial cells from the external cervical os (Ect1/E6E7) and the cervical cancer cell line HeLa following chemoradiotherapy (RCT) treatment. RCT was simulated by X-ray irradiation at graded doses (Gy) for 30 min, followed by cisplatin (DDP) treatment for 24 h. (B–I) mRNA expression levels of differentially regulated ligand signals in HeLa cells from the Blank, CAMP overexpression (CAMP-OE), CAMP knockdown (CAMP-KD), RCT, RCT + CAMP-OE, and RCT + CAMP-KD groups. (J,K) mRNA expression levels of key ligand–receptor pairs in neutrophils and HeLa cells after co-culture before and after RCT treatment. Expression of the ligands COL1A1 (J) , COL1A2 (K) , and ANXA1 (L) was measured in HeLa cells, whereas expression of the receptors CD44 (M) , FPR1 (N) , and FPR2 (O) was assessed in neutrophils.

    Article Snippet: HeLa and Ect1 cell lines were obtained from ATCC.

    Techniques: In Vitro, Biomarker Discovery, Expressing, Irradiation, Over Expression, Knockdown, Co-Culture Assay