fgfr2 expression constructs sc112364 (OriGene)
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fgfr2 expression constructs sc112364
Fgfr2 Expression Constructs Sc112364, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ect1/FGFR2+(NM_000141)+Human+Untagged+Clone/pm23185502-264-3-18
Average 90 stars, based on 1 article reviews
Fgfr2 Expression Constructs Sc112364, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ect1/FGFR2+(NM_000141)+Human+Untagged+Clone/pm23185502-264-3-18
Average 90 stars, based on 1 article reviews
fgfr2 expression constructs sc112364 - by Bioz Stars,
2026-11
90/100 stars
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1) Product Images from "Differential roles of fibroblast growth factor receptors (FGFR) 1, 2 and 3 in the regulation of S115 breast cancer cell growth."
Article Title: Differential roles of fibroblast growth factor receptors (FGFR) 1, 2 and 3 in the regulation of S115 breast cancer cell growth.
Journal: PloS one
doi: 10.1371/journal.pone.0049970
Figure Legend Snippet: Figure 1. FGFR1, 2 and 3 expression in FGFR-silenced S115 cells (sh cells). Cells were grown in DMEM containing 4% iFBS and 10 nM testosterone. RNA and protein were extracted from three independently cultured sub-confluent cell plates and analyzed using qRT-PCR and immunoblotting, respectively. A) FGFR1-3 mRNA expression in the five original FGFR-silenced cell pools (shR1B, shR1D, shR2IA, shR2ADG and shR3B) compared to the expression level in control cells (shLacZ). FGFR mRNA expression is normalized to cyclophilin B expression. The statistical differences between mRNA levels were tested by non-parametric Mann-Whitney U-test, * P,0.05, ** P,0.01. B) Overall relative FGFR mRNA expression level in sh cells. C) Relative FGFR1IgIIIb/c and FGFR2IgIIIb/c mRNA expression in cells that were chosen for further studies (shR1B, shR2IA and shR3B). D) FGFR protein levels in shR1B, shR2IA and shR3B cells. Immunoprecipitation of FGFR1 was performed using 150 mg of whole cell lysates. Detection of FGFR2 and FGFR3 by western blotting was performed using 20 mg of whole cell lysates. FGFR1 expression in shR1, shR2 and shR3 cells is normalized to the protein expression in shLacZ cells. FGFR2 and FGFR3 expression in shR1, shR2 and shR3 cells are normalized to b-actin expression and thereafter to expression in shLacZ. A representative immunoblot of three independent experiments is shown. doi:10.1371/journal.pone.0049970.g001
Techniques Used: Expressing, Cell Culture, Quantitative RT-PCR, Western Blot, Control, MANN-WHITNEY, Immunoprecipitation
Figure Legend Snippet: Figure 7. Overexpression of FGFR2IgIIIb and FGFR2IgIIIc in shLacZ cells. A) FGFR2 mRNA levels (relative to control-transfected cells (pCMV6-neo)) in FGFR2IgIIIb and FGFR2IgIIIc-transfected cells were analyzed by qRT-PCR. mRNA levels measured 24 h post-transfection are presented in a logarithmic scale. B) FGFR2 protein levels 24 h post-transfection shown by western blotting. C) Relative FGFR1 mRNA expression in FGFR2IgIIIb and FGFRIgIIIc-overexpressing cells 24 h post-transfection. The experiment was repeated twice with similar results. doi:10.1371/journal.pone.0049970.g007
Techniques Used: Over Expression, Control, Transfection, Quantitative RT-PCR, Western Blot, Expressing
Related Articles
Transfection:Article Title: Differential roles of fibroblast growth factor receptors (FGFR) 1, 2 and 3 in the regulation of S115 breast cancer cell growth. Article Snippet: The FGFR inhibitor PD173074 (Pfizer Pharmaceuticals, Ann Arbor, MI) was added 30 minutes prior to FGF-8 treatment. .. FGFR2 Transfections The FGFR2 expression constructs SC112364 and Expressing:Article Title: Differential roles of fibroblast growth factor receptors (FGFR) 1, 2 and 3 in the regulation of S115 breast cancer cell growth. Article Snippet: The FGFR inhibitor PD173074 (Pfizer Pharmaceuticals, Ann Arbor, MI) was added 30 minutes prior to FGF-8 treatment. .. FGFR2 Transfections The FGFR2 expression constructs SC112364 and Construct:Article Title: Differential roles of fibroblast growth factor receptors (FGFR) 1, 2 and 3 in the regulation of S115 breast cancer cell growth. Article Snippet: The FGFR inhibitor PD173074 (Pfizer Pharmaceuticals, Ann Arbor, MI) was added 30 minutes prior to FGF-8 treatment. .. FGFR2 Transfections The FGFR2 expression constructs SC112364 and Control:Article Title: Differential roles of fibroblast growth factor receptors (FGFR) 1, 2 and 3 in the regulation of S115 breast cancer cell growth. Article Snippet: The FGFR inhibitor PD173074 (Pfizer Pharmaceuticals, Ann Arbor, MI) was added 30 minutes prior to FGF-8 treatment. .. FGFR2 Transfections The FGFR2 expression constructs SC112364 and other:Article Title: Differential Roles of Fibroblast Growth Factor Receptors (FGFR) 1, 2 and 3 in the Regulation of S115 Breast Cancer Cell Growth Article Snippet: The FGFR inhibitor PD173074 (Pfizer Pharmaceuticals, Ann Arbor, MI) was added 30 minutes prior to FGF-8 treatment. |
