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avrii  (New England Biolabs)


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    Structured Review

    New England Biolabs avrii
    Avrii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 776 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecori-digested+lambda+zap+ii+vector/AvrII/custom%40r0174%4033463551
    Average 97 stars, based on 776 article reviews
    avrii - by Bioz Stars, 2026-10
    97/100 stars

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    Expressing:

    Article Title: Neuronally sensed oxygen drives behavior and development in human-infective, skin-penetrating nematodes
    Article Snippet: To drive expression in Sst- URX, we used SacI-HF (New England Biolabs, R3156) and AgeI-HF (New England Biolabs, R3552) to clone the Sst-gcy-36 promoter from pBMW15 into pRP08, resulting in pBMW31 ( Sst-gcy-36p::strGCaMP7f:: P2A ::strmScarlet-I::Sst-era-1 3′ UTR). .. To drive expression in Sst- BAG, we used AgeI-HF (New England Biolabs, R3552) and AvrII (New England Biolabs, R0174) to clone the strGCaMP7f:: P2A ::strmScarlet-I cassette from pBMW31 into pNB14, thus creating pBMW66 ( Sst-gcy-9p::strGCaMP7f:: P2A ::strmScarlet-I::Sst-era-1 3′ UTR). ..

    Article Title: Transcriptomic and functional analyses uncover a conserved effector driving genotype-dependent virulence in the Sphaerulina musiva-Populus trichocarpa interaction.
    Article Snippet: .. Subsequently, the plasmid was linearized using AvrII (New England Biolabs Inc., Ipswich, MA) and then transformed into the P. pastoris expression vector using the Pichia EasyComp Transformation Kit (Thermo Fisher, Waltham, MA). ..

    Cloning:

    Article Title: Cooperative and competitive interactions among transcription regulatory elements modulate transcription output
    Article Snippet: .. These barcoded plasmids were subsequently digested with AvrII (NEB, R0174S) and AseI (NEB, R0526S) to allow cloning of sixteen different target promoters. .. Target promoters (sequences available in Supplementary Table 2 (target promoter info)) were PCR-amplified from K562 genomic DNA using PrimeSTAR® GXL DNA Polymerase (TaKaRa, R050B) for 32 cycles with primer pairs engineered to introduce AvrII (CCTAAG) and AseI (ATTAAT) restriction sites, with the order of sites determined by whether the primary transcript of the promoter lies in the sense or antisense orientation (primer sequences are provided in Supplementary Table 3 (primers).

    Over Expression:

    Article Title: Loss of flavonol 3‐ O ‐glucosyltransferase activity confers soybean resistance to leaf‐chewing insects
    Article Snippet: .. Two overexpression vectors used in the complementation assays were built as follows: The coding sequences for Glyma07g14530‐S (the wild‐type, susceptible allele) and Glyma07g14530 ‐R (the resistant, mutant allele) were amplified from leaf DNA isolated from Benning and Benning M respectively, with the primers Asc I‐530‐OE‐F and Avr II‐530‐OE‐R (Table ) using the Phusion HF polymerase (New England Biolabs, Ipswich, MA). ..

    Mutagenesis:

    Article Title: Loss of flavonol 3‐ O ‐glucosyltransferase activity confers soybean resistance to leaf‐chewing insects
    Article Snippet: .. Two overexpression vectors used in the complementation assays were built as follows: The coding sequences for Glyma07g14530‐S (the wild‐type, susceptible allele) and Glyma07g14530 ‐R (the resistant, mutant allele) were amplified from leaf DNA isolated from Benning and Benning M respectively, with the primers Asc I‐530‐OE‐F and Avr II‐530‐OE‐R (Table ) using the Phusion HF polymerase (New England Biolabs, Ipswich, MA). ..

    Article Title: Progressive cognitive impairment and ventricular tachycardia in a boy with biallelic POLG variants and a de novo RYR2 variation.
    Article Snippet: .. The MIP1 fragment carrying the variant of interest was digested with NotI-HF and AvrII (NEB, US) and subcloned in pFL39MIP1 and pFL39MIP1HA digested with the same enzymes, in order to obtain the mip1W87R mutant alleles. ..

    Amplification:

    Article Title: Loss of flavonol 3‐ O ‐glucosyltransferase activity confers soybean resistance to leaf‐chewing insects
    Article Snippet: .. Two overexpression vectors used in the complementation assays were built as follows: The coding sequences for Glyma07g14530‐S (the wild‐type, susceptible allele) and Glyma07g14530 ‐R (the resistant, mutant allele) were amplified from leaf DNA isolated from Benning and Benning M respectively, with the primers Asc I‐530‐OE‐F and Avr II‐530‐OE‐R (Table ) using the Phusion HF polymerase (New England Biolabs, Ipswich, MA). ..

    Article Title: Activation of a gene cluster in Streptomyces lavendulae subsp. lavendulae CCM 3239 induced strevertene production
    Article Snippet: .. The construction of pKasOpAmRT2 involved multiple steps: (1) The bidirectional terminator T5 was excised as a 450-bp Eco RI- Hin dIII (filled in with Klenow enzyme) fragment from plasmid pBSBR5 (Javorova et al. ) and inserted into pKasOpAmRb digested with Nsi I (filled in with Klenow enzyme) and Eco RI, yielding pKasOpAmRT1. (2) A 1.7-kb Spe I- Eco RV fragment from pGUS (Myronovskyi et al. ) was cloned into pAPH3 (Csolleiova et al. ) digested with Xba I and Stu I to generate pAPH4. (3) A 3-kb Spe I- Avr II (filled in with Klenow enzyme) fragment from pAPH4 was cloned into pSB40N (Kormanec and Sevcikova ) digested with Spe I and Eco RV, resulting in pAPH4B. (4) The AprR aac3(IV) gene with oriT from plasmid pIJ773 (Gust et al. ) was amplified by PCR using high-fidelity Q5 DNA polymerase (New England Biolabs, San Diego, CA) and primers AmR1 and AmR2 to introduce flanking polylinker sites (Table ). ..

    Isolation:

    Article Title: Loss of flavonol 3‐ O ‐glucosyltransferase activity confers soybean resistance to leaf‐chewing insects
    Article Snippet: .. Two overexpression vectors used in the complementation assays were built as follows: The coding sequences for Glyma07g14530‐S (the wild‐type, susceptible allele) and Glyma07g14530 ‐R (the resistant, mutant allele) were amplified from leaf DNA isolated from Benning and Benning M respectively, with the primers Asc I‐530‐OE‐F and Avr II‐530‐OE‐R (Table ) using the Phusion HF polymerase (New England Biolabs, Ipswich, MA). ..

    Variant Assay:

    Article Title: Progressive cognitive impairment and ventricular tachycardia in a boy with biallelic POLG variants and a de novo RYR2 variation.
    Article Snippet: .. The MIP1 fragment carrying the variant of interest was digested with NotI-HF and AvrII (NEB, US) and subcloned in pFL39MIP1 and pFL39MIP1HA digested with the same enzymes, in order to obtain the mip1W87R mutant alleles. ..

    Sequencing:

    Article Title: Modified Fc-regions to enhance functional affinity of antibodies and antigen binding fragments thereof
    Article Snippet: .. Following sequence confirmation and maxiprep, 15 μg of plasmid DNA was digested with AfeI and AvrII (both NEB) and the insert gel purified. ..

    Plasmid Preparation:

    Article Title: Modified Fc-regions to enhance functional affinity of antibodies and antigen binding fragments thereof
    Article Snippet: .. Following sequence confirmation and maxiprep, 15 μg of plasmid DNA was digested with AfeI and AvrII (both NEB) and the insert gel purified. ..

    Article Title: Transcriptomic and functional analyses uncover a conserved effector driving genotype-dependent virulence in the Sphaerulina musiva-Populus trichocarpa interaction.
    Article Snippet: .. Subsequently, the plasmid was linearized using AvrII (New England Biolabs Inc., Ipswich, MA) and then transformed into the P. pastoris expression vector using the Pichia EasyComp Transformation Kit (Thermo Fisher, Waltham, MA). ..

    Article Title: Activation of a gene cluster in Streptomyces lavendulae subsp. lavendulae CCM 3239 induced strevertene production
    Article Snippet: .. The construction of pKasOpAmRT2 involved multiple steps: (1) The bidirectional terminator T5 was excised as a 450-bp Eco RI- Hin dIII (filled in with Klenow enzyme) fragment from plasmid pBSBR5 (Javorova et al. ) and inserted into pKasOpAmRb digested with Nsi I (filled in with Klenow enzyme) and Eco RI, yielding pKasOpAmRT1. (2) A 1.7-kb Spe I- Eco RV fragment from pGUS (Myronovskyi et al. ) was cloned into pAPH3 (Csolleiova et al. ) digested with Xba I and Stu I to generate pAPH4. (3) A 3-kb Spe I- Avr II (filled in with Klenow enzyme) fragment from pAPH4 was cloned into pSB40N (Kormanec and Sevcikova ) digested with Spe I and Eco RV, resulting in pAPH4B. (4) The AprR aac3(IV) gene with oriT from plasmid pIJ773 (Gust et al. ) was amplified by PCR using high-fidelity Q5 DNA polymerase (New England Biolabs, San Diego, CA) and primers AmR1 and AmR2 to introduce flanking polylinker sites (Table ). ..

    Purification:

    Article Title: Modified Fc-regions to enhance functional affinity of antibodies and antigen binding fragments thereof
    Article Snippet: .. Following sequence confirmation and maxiprep, 15 μg of plasmid DNA was digested with AfeI and AvrII (both NEB) and the insert gel purified. ..

    Transformation Assay:

    Article Title: Transcriptomic and functional analyses uncover a conserved effector driving genotype-dependent virulence in the Sphaerulina musiva-Populus trichocarpa interaction.
    Article Snippet: .. Subsequently, the plasmid was linearized using AvrII (New England Biolabs Inc., Ipswich, MA) and then transformed into the P. pastoris expression vector using the Pichia EasyComp Transformation Kit (Thermo Fisher, Waltham, MA). ..

    Clone Assay:

    Article Title: Activation of a gene cluster in Streptomyces lavendulae subsp. lavendulae CCM 3239 induced strevertene production
    Article Snippet: .. The construction of pKasOpAmRT2 involved multiple steps: (1) The bidirectional terminator T5 was excised as a 450-bp Eco RI- Hin dIII (filled in with Klenow enzyme) fragment from plasmid pBSBR5 (Javorova et al. ) and inserted into pKasOpAmRb digested with Nsi I (filled in with Klenow enzyme) and Eco RI, yielding pKasOpAmRT1. (2) A 1.7-kb Spe I- Eco RV fragment from pGUS (Myronovskyi et al. ) was cloned into pAPH3 (Csolleiova et al. ) digested with Xba I and Stu I to generate pAPH4. (3) A 3-kb Spe I- Avr II (filled in with Klenow enzyme) fragment from pAPH4 was cloned into pSB40N (Kormanec and Sevcikova ) digested with Spe I and Eco RV, resulting in pAPH4B. (4) The AprR aac3(IV) gene with oriT from plasmid pIJ773 (Gust et al. ) was amplified by PCR using high-fidelity Q5 DNA polymerase (New England Biolabs, San Diego, CA) and primers AmR1 and AmR2 to introduce flanking polylinker sites (Table ). ..

    Polymerase Chain Reaction:

    Article Title: Activation of a gene cluster in Streptomyces lavendulae subsp. lavendulae CCM 3239 induced strevertene production
    Article Snippet: .. The construction of pKasOpAmRT2 involved multiple steps: (1) The bidirectional terminator T5 was excised as a 450-bp Eco RI- Hin dIII (filled in with Klenow enzyme) fragment from plasmid pBSBR5 (Javorova et al. ) and inserted into pKasOpAmRb digested with Nsi I (filled in with Klenow enzyme) and Eco RI, yielding pKasOpAmRT1. (2) A 1.7-kb Spe I- Eco RV fragment from pGUS (Myronovskyi et al. ) was cloned into pAPH3 (Csolleiova et al. ) digested with Xba I and Stu I to generate pAPH4. (3) A 3-kb Spe I- Avr II (filled in with Klenow enzyme) fragment from pAPH4 was cloned into pSB40N (Kormanec and Sevcikova ) digested with Spe I and Eco RV, resulting in pAPH4B. (4) The AprR aac3(IV) gene with oriT from plasmid pIJ773 (Gust et al. ) was amplified by PCR using high-fidelity Q5 DNA polymerase (New England Biolabs, San Diego, CA) and primers AmR1 and AmR2 to introduce flanking polylinker sites (Table ). ..

    Introduce:

    Article Title: Activation of a gene cluster in Streptomyces lavendulae subsp. lavendulae CCM 3239 induced strevertene production
    Article Snippet: .. The construction of pKasOpAmRT2 involved multiple steps: (1) The bidirectional terminator T5 was excised as a 450-bp Eco RI- Hin dIII (filled in with Klenow enzyme) fragment from plasmid pBSBR5 (Javorova et al. ) and inserted into pKasOpAmRb digested with Nsi I (filled in with Klenow enzyme) and Eco RI, yielding pKasOpAmRT1. (2) A 1.7-kb Spe I- Eco RV fragment from pGUS (Myronovskyi et al. ) was cloned into pAPH3 (Csolleiova et al. ) digested with Xba I and Stu I to generate pAPH4. (3) A 3-kb Spe I- Avr II (filled in with Klenow enzyme) fragment from pAPH4 was cloned into pSB40N (Kormanec and Sevcikova ) digested with Spe I and Eco RV, resulting in pAPH4B. (4) The AprR aac3(IV) gene with oriT from plasmid pIJ773 (Gust et al. ) was amplified by PCR using high-fidelity Q5 DNA polymerase (New England Biolabs, San Diego, CA) and primers AmR1 and AmR2 to introduce flanking polylinker sites (Table ). ..



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