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dapi  (Beyotime)


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    Structured Review

    Beyotime dapi
    Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32727 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ts2-fl+microscope/DAPI/pm42786917-164-8-9
    Average 99 stars, based on 32727 article reviews
    dapi - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Aged ZnO Nanoparticles Promote Breast Cancer Lung Metastasis by Remodeling the Pulmonary Microenvironment Through the CXCL10/Wnt/β‐Catenin Axis
    Article Snippet: NIH‐3T3 cells, RAW264.7 cells, or 4T1/MCF7 cells treated with 40 ng mL −1 rCXCL10 were seeded onto the coverslips and cultured for 72 h. Cells were fixed with 4% paraformaldehyde for 15 min, washed with PBS, permeabilized with 0.2% Triton X‐100 (Solarbio, T8200) for 5 min, and blocked with 5% BSA (Solarbio, A8850) for 2 h. For tissue sections, slides underwent deparaffinization, rehydration, and blocking with 5% BSA for 2 h. Both coverslips and tissue sections were incubated overnight at 4°C with primary antibodies against α‐SMA (Affinity, AF1032), CD206 (Affinity, DF4149), or β‐Catenin (Proteintech, PRO‐CY‐50WY). .. After three washes, samples were incubated for 1 h at room temperature in the dark with Fluor488‐conjugated secondary antibody (Immunoway, RS32109), counterstained with DAPI (Beyotime, C1005) for 3 min, and mounted with antifade medium (Abbkine, BMU104). ..

    Lysis:

    Article Title: A Nitric Oxide–Based STING Agonist Nanoadjuvant With ICD‐Derived Membrane Antigens Unleashes Robust Antitumor Immunity
    Article Snippet: Mouse ELISA kits for quantifying IL‐6, IL‐1β, TNF‐α, and IFN‐β were supplied by Beijing Solarbio Science & Technology Co., Ltd. (China). .. DAPI, PMSF, and RIPA lysis buffer were acquired from Beyotime Biotechnology (China). .. Antibodies targeting phospho‐TBK1 (Ser172, #5483), phospho‐STING (Ser365, #72971), total TBK1 (#38066), and total STING (#13647) were purchased from Cell Signaling Technology (CST) via Beijing Chengzhi Kewei Biotechnology Co., Ltd. (China).

    Fluorescence:

    Article Title: ATG5-overexpressing MSCs improve ligament healing with an associated shift toward M2 macrophage polarization in aged rats
    Article Snippet: .. Nuclei were counterstained with DAPI (Beyotime, China) for 5 min. Coverslips were mounted, and images were obtained using a Leica fluorescence microscope. ..

    Microscopy:

    Article Title: ATG5-overexpressing MSCs improve ligament healing with an associated shift toward M2 macrophage polarization in aged rats
    Article Snippet: .. Nuclei were counterstained with DAPI (Beyotime, China) for 5 min. Coverslips were mounted, and images were obtained using a Leica fluorescence microscope. ..

    Staining:

    Article Title: IL-4 and arginine synergy accelerates early bone regeneration via enhanced efferocytosis and ameliorated microenvironment
    Article Snippet: .. Nuclei were counterstained with DAPI (Beyotime) for 10 min. All staining was performed at room temperature in the dark. .. Finally, the fluorescence images of the stained cells were obtained using a confocal laser scanning microscope (Leica TCS SP8, Leica Microsystems, Wetzlar, Germany) and analyzed with ImageJ software.



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    KY-05009 impairs platelet tethering to immobilized fibrinogen. Adhesion of (A) vehicle (0.5% v/v dimethylsulfoxide [DMSO]) or (B) KY-05009 (10 μM)-treated platelets (4 × 10 7 cells/mL) to fibrinogen (100 μg/mL). F-actin was stained with Alexa Fluor 568 phalloidin (1:100 dilution). Platelets were visualized with a Nikon Eclipse <t>Ts2</t> Fl <t>microscope</t> using a 40X/0.65 objective. Representative images are shown: (C) percentage platelet coverage and (D) mean data; n = 4. Data are presented as mean ± SEM. Data analyzed by 1-way ANOVA; ∗ P < .03.
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    Image Search Results


    KY-05009 impairs platelet tethering to immobilized fibrinogen. Adhesion of (A) vehicle (0.5% v/v dimethylsulfoxide [DMSO]) or (B) KY-05009 (10 μM)-treated platelets (4 × 10 7 cells/mL) to fibrinogen (100 μg/mL). F-actin was stained with Alexa Fluor 568 phalloidin (1:100 dilution). Platelets were visualized with a Nikon Eclipse Ts2 Fl microscope using a 40X/0.65 objective. Representative images are shown: (C) percentage platelet coverage and (D) mean data; n = 4. Data are presented as mean ± SEM. Data analyzed by 1-way ANOVA; ∗ P < .03.

    Journal: Research and Practice in Thrombosis and Haemostasis

    Article Title: TRAF2 and NCK interacting kinase: a novel regulator of integrin α IIb β 3 signaling in platelets

    doi: 10.1016/j.rpth.2025.103204

    Figure Lengend Snippet: KY-05009 impairs platelet tethering to immobilized fibrinogen. Adhesion of (A) vehicle (0.5% v/v dimethylsulfoxide [DMSO]) or (B) KY-05009 (10 μM)-treated platelets (4 × 10 7 cells/mL) to fibrinogen (100 μg/mL). F-actin was stained with Alexa Fluor 568 phalloidin (1:100 dilution). Platelets were visualized with a Nikon Eclipse Ts2 Fl microscope using a 40X/0.65 objective. Representative images are shown: (C) percentage platelet coverage and (D) mean data; n = 4. Data are presented as mean ± SEM. Data analyzed by 1-way ANOVA; ∗ P < .03.

    Article Snippet: Representative images were taken using a Nikon Eclipse Ts2 Fl microscope with a 40X/0.65 objective.

    Techniques: Staining, Microscopy

    KY-05009 treatment attenuates thrombus formation under arterial shear stress. DIOC6 labeled whole blood was treated with (A) vehicle (DMSO, 1% v/v), (B) KY-05009 (10 μM), (C) KY-05009 (50 μM), or (D) KY-05009 (100 μM) and perfused over collagen coated (100μg/mL) Vena8 Fluoro+ Biochips using a Cellix perfusion system at arterial sheer stress (20 dynes/cm 2 ) for 10 minutes. Thrombus formation was determined by monitoring the change in fluorescence intensity observed over recorded intervals. Representative images were taken using a Nikon Eclipse Ts2 Fl microscope with a 40X/0.65 objective. The fluorescence intensity of the formed thrombi were normalized to the maximum fluorescence intensity value of the vehicle after 10 minutes and expressed as a percentage; n = 6. Decimated averages at each time point are presented ± SEM. (E) Annexin V binding to platelets treated with vehicle (DMSO, 1% v/v) or KY-005009 (10-100 μM). Platelets were gated and 10 000 events were recorded. Mean ± SEM of raw median fluorescence intensity data are presented; n = 3. Thrombus formation data analyzed by 2-way ANOVA; flow cytometry data analyzed by 1-way ANOVA; ∗ P < .03.

    Journal: Research and Practice in Thrombosis and Haemostasis

    Article Title: TRAF2 and NCK interacting kinase: a novel regulator of integrin α IIb β 3 signaling in platelets

    doi: 10.1016/j.rpth.2025.103204

    Figure Lengend Snippet: KY-05009 treatment attenuates thrombus formation under arterial shear stress. DIOC6 labeled whole blood was treated with (A) vehicle (DMSO, 1% v/v), (B) KY-05009 (10 μM), (C) KY-05009 (50 μM), or (D) KY-05009 (100 μM) and perfused over collagen coated (100μg/mL) Vena8 Fluoro+ Biochips using a Cellix perfusion system at arterial sheer stress (20 dynes/cm 2 ) for 10 minutes. Thrombus formation was determined by monitoring the change in fluorescence intensity observed over recorded intervals. Representative images were taken using a Nikon Eclipse Ts2 Fl microscope with a 40X/0.65 objective. The fluorescence intensity of the formed thrombi were normalized to the maximum fluorescence intensity value of the vehicle after 10 minutes and expressed as a percentage; n = 6. Decimated averages at each time point are presented ± SEM. (E) Annexin V binding to platelets treated with vehicle (DMSO, 1% v/v) or KY-005009 (10-100 μM). Platelets were gated and 10 000 events were recorded. Mean ± SEM of raw median fluorescence intensity data are presented; n = 3. Thrombus formation data analyzed by 2-way ANOVA; flow cytometry data analyzed by 1-way ANOVA; ∗ P < .03.

    Article Snippet: Representative images were taken using a Nikon Eclipse Ts2 Fl microscope with a 40X/0.65 objective.

    Techniques: Shear, Labeling, Fluorescence, Microscopy, Binding Assay, Flow Cytometry