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dapi  (Beyotime)


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    Structured Review

    Beyotime dapi
    Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 34692 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ts2-fl+microscope/DAPI/pm42786917-164-8-9
    Average 99 stars, based on 34692 article reviews
    dapi - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Staining:

    Article Title: SATB1 lowers the T cell receptor signaling threshold for thymocyte positive selection via super-enhancer reprogramming.
    Article Snippet: Followed by 3 × PBS wash, followed by incubation with a secondary antibody in conjunctionwithAlexa 647fluorescence (FD0144) 1:1000 in 1 × PBS for onehour in the dark. .. Afterwashing three times, the nuclei were stained with 10 μg/ml DAPI (Beyotime). .. Images were acquired by a confocal microscope (Carl Zeiss, LSM 880 with Airyscan or Nikon A1 R) with a 100x objective.

    Article Title: CD147 drives keloid formation by promoting fibroblast proliferation via the CENPF signaling pathway.
    Article Snippet: After being inoculated onto 24-well culture plates and subjected to the indicated treatments, the CoraLite488 TUNEL assay apoptosis detection kit (Proteintech, Wuhan, China) was used to detect apoptotic cells according to the manufacturer’s instructions. .. Nuclei were stained with 4′,6- diamidino-2-phenylindole (DAPI, Beyotime, Shanghai, China) for 5 min. TUNEL-positive apoptotic cells were labeled with green fluorescence and visualized by a Leica Microsystems microscope (Solms, Germany). ..

    Article Title: The AGEs/RAGE Axis Contributes to Myocardial Cell Death in Type 1 Diabetic Cardiomyopathy via the ROS/NF‐ κ B/NLRP3 Inflammasome Pathway
    Article Snippet: β ‐Actin (Cat#R1207‐1) was purchased from Huabio (Harbin, China). .. Trypsin solution (Cat#C0201), CM‐H2DCFDA staining (Cat#S0035S), Cy3‐conjugated secondary antibody (Cat# A0521), FITC‐conjugated secondary antibody (Cat# A0568), 4 ′ ,6‐diamidino‐2‐phenylindole (DAPI) (CatC1005), RIPA (Cat#P0013B), protein SDS‐PAGE protein loading buffer (5X) (Cat#P0015), BCA protein concentration measurement kit (Cat#P0011), and lactate dehydrogenase (LDH) cytotoxicity assay kit (Cat#C0016) were purchased from Beyotime (Beijing, China). .. α ‐Actinin (Cat#GTX11007) was from GeneTex (Texas, United States).

    Blocking Assay:

    Article Title: NSUN3‐Associated m5C Modification of tRF5‐GlyTCC Suppresses Colorectal Cancer Progression via RPL23‐Linked Nucleolar Stress and p53‐Associated Autophagy and Enhances 5‐FU Sensitivity
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde for 15 min and permeabilized with 0.1% Triton X‐100 for 10 min. After blocking with 5% BSA for 1 h, cells were incubated with an anti‐NPM1 antibody (Abcam, UK) overnight at 4°C, followed by incubation with an Alexa Fluor‐conjugated secondary antibody for 1 h. Nuclei were counterstained with DAPI (Beyotime, China). ..

    Incubation:

    Article Title: NSUN3‐Associated m5C Modification of tRF5‐GlyTCC Suppresses Colorectal Cancer Progression via RPL23‐Linked Nucleolar Stress and p53‐Associated Autophagy and Enhances 5‐FU Sensitivity
    Article Snippet: .. Cells were fixed with 4% paraformaldehyde for 15 min and permeabilized with 0.1% Triton X‐100 for 10 min. After blocking with 5% BSA for 1 h, cells were incubated with an anti‐NPM1 antibody (Abcam, UK) overnight at 4°C, followed by incubation with an Alexa Fluor‐conjugated secondary antibody for 1 h. Nuclei were counterstained with DAPI (Beyotime, China). ..

    Article Title: Lineage-specific and gestational age–dependent DNA methylation programming orchestrates human placental development
    Article Snippet: Then, they were incubated with 5mC (61255, Active Motif), EGFR (ab30, Abcam), HLA-G (ab52454, Abcam), TFAP2C (sc12762, Santa Cruz Biotechnology), CDH2 (ab18203, Abcam), EpCAM (MA5-12436, Thermo Fisher Scientific), TP63 (ab124762, Abcam), SDC1 (67155-1-Ig, Proteintech), CGB (ab9582, Abcam), or α-SMA (MA1-06110, Thermo Fisher Scientific) overnight at 4°C. .. After multiple washes to remove residual primary antibody, cells or sections were then incubated with AF488/AF594-conjugated secondary antibodies (Thermo Fisher Scientific) for 1 hour at room temperature and mounted in prolonged anti-fade reagent with 4′,6-diamidino-2-phenylindole (DAPI, Beyotime). .. Confocal imaging was performed with Zeiss LSM 880 confocal microscopes or LEICA TCS SP8, and analyzed with Zeiss Zen blue edition.

    TUNEL Assay:

    Article Title: CD147 drives keloid formation by promoting fibroblast proliferation via the CENPF signaling pathway.
    Article Snippet: After being inoculated onto 24-well culture plates and subjected to the indicated treatments, the CoraLite488 TUNEL assay apoptosis detection kit (Proteintech, Wuhan, China) was used to detect apoptotic cells according to the manufacturer’s instructions. .. Nuclei were stained with 4′,6- diamidino-2-phenylindole (DAPI, Beyotime, Shanghai, China) for 5 min. TUNEL-positive apoptotic cells were labeled with green fluorescence and visualized by a Leica Microsystems microscope (Solms, Germany). ..

    Labeling:

    Article Title: CD147 drives keloid formation by promoting fibroblast proliferation via the CENPF signaling pathway.
    Article Snippet: After being inoculated onto 24-well culture plates and subjected to the indicated treatments, the CoraLite488 TUNEL assay apoptosis detection kit (Proteintech, Wuhan, China) was used to detect apoptotic cells according to the manufacturer’s instructions. .. Nuclei were stained with 4′,6- diamidino-2-phenylindole (DAPI, Beyotime, Shanghai, China) for 5 min. TUNEL-positive apoptotic cells were labeled with green fluorescence and visualized by a Leica Microsystems microscope (Solms, Germany). ..

    Fluorescence:

    Article Title: CD147 drives keloid formation by promoting fibroblast proliferation via the CENPF signaling pathway.
    Article Snippet: After being inoculated onto 24-well culture plates and subjected to the indicated treatments, the CoraLite488 TUNEL assay apoptosis detection kit (Proteintech, Wuhan, China) was used to detect apoptotic cells according to the manufacturer’s instructions. .. Nuclei were stained with 4′,6- diamidino-2-phenylindole (DAPI, Beyotime, Shanghai, China) for 5 min. TUNEL-positive apoptotic cells were labeled with green fluorescence and visualized by a Leica Microsystems microscope (Solms, Germany). ..

    Microscopy:

    Article Title: CD147 drives keloid formation by promoting fibroblast proliferation via the CENPF signaling pathway.
    Article Snippet: After being inoculated onto 24-well culture plates and subjected to the indicated treatments, the CoraLite488 TUNEL assay apoptosis detection kit (Proteintech, Wuhan, China) was used to detect apoptotic cells according to the manufacturer’s instructions. .. Nuclei were stained with 4′,6- diamidino-2-phenylindole (DAPI, Beyotime, Shanghai, China) for 5 min. TUNEL-positive apoptotic cells were labeled with green fluorescence and visualized by a Leica Microsystems microscope (Solms, Germany). ..

    BIA-KA:

    Article Title: The AGEs/RAGE Axis Contributes to Myocardial Cell Death in Type 1 Diabetic Cardiomyopathy via the ROS/NF‐ κ B/NLRP3 Inflammasome Pathway
    Article Snippet: β ‐Actin (Cat#R1207‐1) was purchased from Huabio (Harbin, China). .. Trypsin solution (Cat#C0201), CM‐H2DCFDA staining (Cat#S0035S), Cy3‐conjugated secondary antibody (Cat# A0521), FITC‐conjugated secondary antibody (Cat# A0568), 4 ′ ,6‐diamidino‐2‐phenylindole (DAPI) (CatC1005), RIPA (Cat#P0013B), protein SDS‐PAGE protein loading buffer (5X) (Cat#P0015), BCA protein concentration measurement kit (Cat#P0011), and lactate dehydrogenase (LDH) cytotoxicity assay kit (Cat#C0016) were purchased from Beyotime (Beijing, China). .. α ‐Actinin (Cat#GTX11007) was from GeneTex (Texas, United States).

    Protein Concentration:

    Article Title: The AGEs/RAGE Axis Contributes to Myocardial Cell Death in Type 1 Diabetic Cardiomyopathy via the ROS/NF‐ κ B/NLRP3 Inflammasome Pathway
    Article Snippet: β ‐Actin (Cat#R1207‐1) was purchased from Huabio (Harbin, China). .. Trypsin solution (Cat#C0201), CM‐H2DCFDA staining (Cat#S0035S), Cy3‐conjugated secondary antibody (Cat# A0521), FITC‐conjugated secondary antibody (Cat# A0568), 4 ′ ,6‐diamidino‐2‐phenylindole (DAPI) (CatC1005), RIPA (Cat#P0013B), protein SDS‐PAGE protein loading buffer (5X) (Cat#P0015), BCA protein concentration measurement kit (Cat#P0011), and lactate dehydrogenase (LDH) cytotoxicity assay kit (Cat#C0016) were purchased from Beyotime (Beijing, China). .. α ‐Actinin (Cat#GTX11007) was from GeneTex (Texas, United States).

    LDH Cytotoxicity Assay:

    Article Title: The AGEs/RAGE Axis Contributes to Myocardial Cell Death in Type 1 Diabetic Cardiomyopathy via the ROS/NF‐ κ B/NLRP3 Inflammasome Pathway
    Article Snippet: β ‐Actin (Cat#R1207‐1) was purchased from Huabio (Harbin, China). .. Trypsin solution (Cat#C0201), CM‐H2DCFDA staining (Cat#S0035S), Cy3‐conjugated secondary antibody (Cat# A0521), FITC‐conjugated secondary antibody (Cat# A0568), 4 ′ ,6‐diamidino‐2‐phenylindole (DAPI) (CatC1005), RIPA (Cat#P0013B), protein SDS‐PAGE protein loading buffer (5X) (Cat#P0015), BCA protein concentration measurement kit (Cat#P0011), and lactate dehydrogenase (LDH) cytotoxicity assay kit (Cat#C0016) were purchased from Beyotime (Beijing, China). .. α ‐Actinin (Cat#GTX11007) was from GeneTex (Texas, United States).



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    KY-05009 impairs platelet tethering to immobilized fibrinogen. Adhesion of (A) vehicle (0.5% v/v dimethylsulfoxide [DMSO]) or (B) KY-05009 (10 μM)-treated platelets (4 × 10 7 cells/mL) to fibrinogen (100 μg/mL). F-actin was stained with Alexa Fluor 568 phalloidin (1:100 dilution). Platelets were visualized with a Nikon Eclipse <t>Ts2</t> Fl <t>microscope</t> using a 40X/0.65 objective. Representative images are shown: (C) percentage platelet coverage and (D) mean data; n = 4. Data are presented as mean ± SEM. Data analyzed by 1-way ANOVA; ∗ P < .03.
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    Image Search Results


    KY-05009 impairs platelet tethering to immobilized fibrinogen. Adhesion of (A) vehicle (0.5% v/v dimethylsulfoxide [DMSO]) or (B) KY-05009 (10 μM)-treated platelets (4 × 10 7 cells/mL) to fibrinogen (100 μg/mL). F-actin was stained with Alexa Fluor 568 phalloidin (1:100 dilution). Platelets were visualized with a Nikon Eclipse Ts2 Fl microscope using a 40X/0.65 objective. Representative images are shown: (C) percentage platelet coverage and (D) mean data; n = 4. Data are presented as mean ± SEM. Data analyzed by 1-way ANOVA; ∗ P < .03.

    Journal: Research and Practice in Thrombosis and Haemostasis

    Article Title: TRAF2 and NCK interacting kinase: a novel regulator of integrin α IIb β 3 signaling in platelets

    doi: 10.1016/j.rpth.2025.103204

    Figure Lengend Snippet: KY-05009 impairs platelet tethering to immobilized fibrinogen. Adhesion of (A) vehicle (0.5% v/v dimethylsulfoxide [DMSO]) or (B) KY-05009 (10 μM)-treated platelets (4 × 10 7 cells/mL) to fibrinogen (100 μg/mL). F-actin was stained with Alexa Fluor 568 phalloidin (1:100 dilution). Platelets were visualized with a Nikon Eclipse Ts2 Fl microscope using a 40X/0.65 objective. Representative images are shown: (C) percentage platelet coverage and (D) mean data; n = 4. Data are presented as mean ± SEM. Data analyzed by 1-way ANOVA; ∗ P < .03.

    Article Snippet: Representative images were taken using a Nikon Eclipse Ts2 Fl microscope with a 40X/0.65 objective.

    Techniques: Staining, Microscopy

    KY-05009 treatment attenuates thrombus formation under arterial shear stress. DIOC6 labeled whole blood was treated with (A) vehicle (DMSO, 1% v/v), (B) KY-05009 (10 μM), (C) KY-05009 (50 μM), or (D) KY-05009 (100 μM) and perfused over collagen coated (100μg/mL) Vena8 Fluoro+ Biochips using a Cellix perfusion system at arterial sheer stress (20 dynes/cm 2 ) for 10 minutes. Thrombus formation was determined by monitoring the change in fluorescence intensity observed over recorded intervals. Representative images were taken using a Nikon Eclipse Ts2 Fl microscope with a 40X/0.65 objective. The fluorescence intensity of the formed thrombi were normalized to the maximum fluorescence intensity value of the vehicle after 10 minutes and expressed as a percentage; n = 6. Decimated averages at each time point are presented ± SEM. (E) Annexin V binding to platelets treated with vehicle (DMSO, 1% v/v) or KY-005009 (10-100 μM). Platelets were gated and 10 000 events were recorded. Mean ± SEM of raw median fluorescence intensity data are presented; n = 3. Thrombus formation data analyzed by 2-way ANOVA; flow cytometry data analyzed by 1-way ANOVA; ∗ P < .03.

    Journal: Research and Practice in Thrombosis and Haemostasis

    Article Title: TRAF2 and NCK interacting kinase: a novel regulator of integrin α IIb β 3 signaling in platelets

    doi: 10.1016/j.rpth.2025.103204

    Figure Lengend Snippet: KY-05009 treatment attenuates thrombus formation under arterial shear stress. DIOC6 labeled whole blood was treated with (A) vehicle (DMSO, 1% v/v), (B) KY-05009 (10 μM), (C) KY-05009 (50 μM), or (D) KY-05009 (100 μM) and perfused over collagen coated (100μg/mL) Vena8 Fluoro+ Biochips using a Cellix perfusion system at arterial sheer stress (20 dynes/cm 2 ) for 10 minutes. Thrombus formation was determined by monitoring the change in fluorescence intensity observed over recorded intervals. Representative images were taken using a Nikon Eclipse Ts2 Fl microscope with a 40X/0.65 objective. The fluorescence intensity of the formed thrombi were normalized to the maximum fluorescence intensity value of the vehicle after 10 minutes and expressed as a percentage; n = 6. Decimated averages at each time point are presented ± SEM. (E) Annexin V binding to platelets treated with vehicle (DMSO, 1% v/v) or KY-005009 (10-100 μM). Platelets were gated and 10 000 events were recorded. Mean ± SEM of raw median fluorescence intensity data are presented; n = 3. Thrombus formation data analyzed by 2-way ANOVA; flow cytometry data analyzed by 1-way ANOVA; ∗ P < .03.

    Article Snippet: Representative images were taken using a Nikon Eclipse Ts2 Fl microscope with a 40X/0.65 objective.

    Techniques: Shear, Labeling, Fluorescence, Microscopy, Binding Assay, Flow Cytometry