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eclipse ti e c2 microscope  (Nikon)


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    Structured Review

    Nikon eclipse ti e c2 microscope
    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
    Eclipse Ti E C2 Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ti+microscope/C2%2B/pmc12811640-185-7-6
    Average 99 stars, based on 1 article reviews
    eclipse ti e c2 microscope - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7"

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    Journal: Biofilm

    doi: 10.1016/j.bioflm.2025.100335

    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.
    Figure Legend Snippet: Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Techniques Used: Staining, Mutagenesis, Fluorescence, Microscopy

    Related Articles

    other:

    Article Title: Hepatocyte IER2 attenuates APAP-induced liver injury by regulating ACOT2-mediated lipid metabolism in mice.
    Article Snippet: TUNELstained pathological staining sections were observed under a fluorescence microscope (Eclipse C1; Nikon).

    Article Title: Electroacupuncture Ameliorates Alcohol‐Related Cognitive Impairment by Modulating the Gut‐Brain Axis to Alleviate LPS Driven Neuroinflammation
    Article Snippet: Frozen brain sections were observed under a laser confocal microscope (FV1200, Olympus), and the immunofluorescence‐stained paraffin jejunal sections were examined under a fluorescence microscope (ECLIPSE C1, Nikon).

    Article Title: Endothelial exosomes remodel chromatin accessibility and superenhancer in cancer cells to promote liver metastatic colonization.
    Article Snippet: Labeled exosomes were added to the Mel270-GFP, Omm2.3GFP, and Mel270-HM3 subline#1-GFP cells for 24 h, which were subsequently visualized by confocal microscopy (Nikon C2, Tokyo, Japan).

    Confocal:

    Article Title: An oxygen-releasing mouthwash shows species-dependent antibacterial and antibiofilm activity against periodontal pathogens
    Article Snippet: .. Z-stacks were acquired using a Nikon C2 confocal microscope (Nikon Instruments Inc., Tokyo, Japan), and biofilm biomass and thickness were quantified separately using COMSTAT2 (version 2.1) [ , ]. ..

    Article Title: Early spinogenesis in postnatally-born hippocampal granule cells.
    Article Snippet: Finally, sections were washed 4 times with 0.1 M TBS containing 0.01% NaN3 and mounted with DAKO fluorescent mounting medium (Dako Cytomation, Carpinteria, CA, USA). .. To select suitable candidate cells for further analysis, 20 x and 40 x magnification image stacks were acquired with a confocal laser scanning microscope (Nikon D-Eclipse C2 controlled by Nikon EZ-C1 v3.60 software, equipped either with Nikon Plan Fluor 40 x, NA 1.3 or Plan Apo 20 ×, NA 0.75 objectives, all from Nikon Instruments, Amstelveen, the Netherlands). ..

    Article Title: Female iPSC X chromosome inactivation erosion and its transcriptomic effects during CRISPR gene editing and neural differentiation.
    Article Snippet: The following antibodies were used: rabbit anti-Oct4 (1:250) (abcam #ab181557), mouse anti-SSEA4 (1:250; abcam #ab16287), goat anti-Human Nanog (1:20; R&D Systems #AF1997), donkey anti-Rabbit IgG Alexa Fluor Plus 647 (Fisher Scientific #A32795), donkey anti-Mouse IgG Alexa Fluor Plus 594 (Fisher Scientific #A32744), and chicken anti-Goat IgG Alexa Fluor 488 (Fisher Scientific #A21467). .. Confocal images were acquired using a Nikon C2 Confocal Microscope with a 20× objective. .. Lentiviral vectors were generated by transfecting HEK293T cells with 3rd generation lentivirus packaging plasmids (pMDLg/pRRE, VSV-G, and pRSV-REV) with the desired vectors using lipofectamine 3000 (Invitrogen #L3000015).

    Microscopy:

    Article Title: An oxygen-releasing mouthwash shows species-dependent antibacterial and antibiofilm activity against periodontal pathogens
    Article Snippet: .. Z-stacks were acquired using a Nikon C2 confocal microscope (Nikon Instruments Inc., Tokyo, Japan), and biofilm biomass and thickness were quantified separately using COMSTAT2 (version 2.1) [ , ]. ..

    Article Title: Basis of the IgM cleavage activity of IgMDE Lsp and IgMDE Mma .
    Article Snippet: Abnormally elevated immunoglobulin M (IgM) levels can cause tissue damage and increase susceptibility to bacterial infections.. Therefore, the use of IgM-degrading enzymes is clinically important for treating diseases characterized by elevated IgM levels.. IgMDELsp and IgMDEMma are two newly discovered highly specific IgMdegrading enzymes.

    Article Title: Female iPSC X chromosome inactivation erosion and its transcriptomic effects during CRISPR gene editing and neural differentiation.
    Article Snippet: The following antibodies were used: rabbit anti-Oct4 (1:250) (abcam #ab181557), mouse anti-SSEA4 (1:250; abcam #ab16287), goat anti-Human Nanog (1:20; R&D Systems #AF1997), donkey anti-Rabbit IgG Alexa Fluor Plus 647 (Fisher Scientific #A32795), donkey anti-Mouse IgG Alexa Fluor Plus 594 (Fisher Scientific #A32744), and chicken anti-Goat IgG Alexa Fluor 488 (Fisher Scientific #A21467). .. Confocal images were acquired using a Nikon C2 Confocal Microscope with a 20× objective. .. Lentiviral vectors were generated by transfecting HEK293T cells with 3rd generation lentivirus packaging plasmids (pMDLg/pRRE, VSV-G, and pRSV-REV) with the desired vectors using lipofectamine 3000 (Invitrogen #L3000015).

    Article Title: Dietary L-Malic Acid Enhances Growth Performance and Oxidative Myofiber Composition in Nursery Piglets.
    Article Snippet: Finally, DAPI (diluted 1:500, C0060, Solarbio, Beijing, China) was applied for nuclear counterstaining, and the sections were incubated with a spontaneous fluorescence-quenching reagent (Solarbio, S2100; Beijing, China). .. Fluorescence images were captured with a Nikon Eclipse C1 microscope (Tokyo, https://doi.org/10.3390/molecules31193381 Japan) and subsequently scanned using a 3D HISTECH Panoramic MIDI slide scanner (3D HISTECH, Budapest, Hungary). .. Slow-twitch (type I) and fast-twitch (type II) fiber percentages were determined with ImageJ software (version 1.54k, National Institutes of Health, Bethesda, MD, USA).

    Analysis:

    Article Title: Early spinogenesis in postnatally-born hippocampal granule cells.
    Article Snippet: Finally, sections were washed 4 times with 0.1 M TBS containing 0.01% NaN3 and mounted with DAKO fluorescent mounting medium (Dako Cytomation, Carpinteria, CA, USA). .. To select suitable candidate cells for further analysis, 20 x and 40 x magnification image stacks were acquired with a confocal laser scanning microscope (Nikon D-Eclipse C2 controlled by Nikon EZ-C1 v3.60 software, equipped either with Nikon Plan Fluor 40 x, NA 1.3 or Plan Apo 20 ×, NA 0.75 objectives, all from Nikon Instruments, Amstelveen, the Netherlands). ..

    Laser-Scanning Microscopy:

    Article Title: Early spinogenesis in postnatally-born hippocampal granule cells.
    Article Snippet: Finally, sections were washed 4 times with 0.1 M TBS containing 0.01% NaN3 and mounted with DAKO fluorescent mounting medium (Dako Cytomation, Carpinteria, CA, USA). .. To select suitable candidate cells for further analysis, 20 x and 40 x magnification image stacks were acquired with a confocal laser scanning microscope (Nikon D-Eclipse C2 controlled by Nikon EZ-C1 v3.60 software, equipped either with Nikon Plan Fluor 40 x, NA 1.3 or Plan Apo 20 ×, NA 0.75 objectives, all from Nikon Instruments, Amstelveen, the Netherlands). ..

    Software:

    Article Title: Early spinogenesis in postnatally-born hippocampal granule cells.
    Article Snippet: Finally, sections were washed 4 times with 0.1 M TBS containing 0.01% NaN3 and mounted with DAKO fluorescent mounting medium (Dako Cytomation, Carpinteria, CA, USA). .. To select suitable candidate cells for further analysis, 20 x and 40 x magnification image stacks were acquired with a confocal laser scanning microscope (Nikon D-Eclipse C2 controlled by Nikon EZ-C1 v3.60 software, equipped either with Nikon Plan Fluor 40 x, NA 1.3 or Plan Apo 20 ×, NA 0.75 objectives, all from Nikon Instruments, Amstelveen, the Netherlands). ..

    Fluorescence:

    Article Title: Basis of the IgM cleavage activity of IgMDE Lsp and IgMDE Mma .
    Article Snippet: Abnormally elevated immunoglobulin M (IgM) levels can cause tissue damage and increase susceptibility to bacterial infections.. Therefore, the use of IgM-degrading enzymes is clinically important for treating diseases characterized by elevated IgM levels.. IgMDELsp and IgMDEMma are two newly discovered highly specific IgMdegrading enzymes.

    Article Title: Dietary L-Malic Acid Enhances Growth Performance and Oxidative Myofiber Composition in Nursery Piglets.
    Article Snippet: Finally, DAPI (diluted 1:500, C0060, Solarbio, Beijing, China) was applied for nuclear counterstaining, and the sections were incubated with a spontaneous fluorescence-quenching reagent (Solarbio, S2100; Beijing, China). .. Fluorescence images were captured with a Nikon Eclipse C1 microscope (Tokyo, https://doi.org/10.3390/molecules31193381 Japan) and subsequently scanned using a 3D HISTECH Panoramic MIDI slide scanner (3D HISTECH, Budapest, Hungary). .. Slow-twitch (type I) and fast-twitch (type II) fiber percentages were determined with ImageJ software (version 1.54k, National Institutes of Health, Bethesda, MD, USA).



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