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eclipse ti e microscope  (Nikon)


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    Nikon eclipse ti e microscope
    Eclipse Ti E Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ti+e+microscope/MM-200/pmc04764664-155-8-7
    Average 96 stars, based on 1 article reviews
    eclipse ti e microscope - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Retrospective evaluation of the reproductive status of male grey wolves (Canis lupus) in Germany based on animals found dead: Influence of age, body weight and season.
    Article Snippet: .. The presence of sperm cells was observed by the same person under the microscope with phase contrast optics and a 200 × magnification (Nikon, Tokyo, Japan). ..

    Article Title: The First of Us: Ophiocordyceps use a novel scramblase-binding peptide to manipulate zombie ants
    Article Snippet: .. Confocal spinning disk microscopy was performed on a Nikon Eclipse Ti2-E microscope equipped with a Yokogawa CSU-X1-M1 spinning disk, an Omicron LightHUB 6 laser box (GFP excitation 488 (4.48 mW), mKate2 excitation 561 (3.18 mW)), Nikon CFI Plan Apo lambda 60x/1.4 NA or 100x/1.45 NA oil immersion objectives, and a PrimeBSI sCMOS camera for signal detection. .. During acquisition the microscope was controlled using MetaMorph software (v7.10; Molecular Devices) and settings were kept constant in animals of each strain.

    Article Title: Macrophage Phenotype-Dependent Protein Corona Formation Governs Ligand Accessibility and Immune Clearance of Biomimetic Nanoparticles.
    Article Snippet: .. To induce M1 polarization, cells were stimulated with 100 ng/mL lipopolysaccharide (LPS) for 24 h. For M2 polarization, cells were treated with 40 ng/mL interleukin-4 (IL-4) for 48 h. Cellular morphology and phenotypic changes were observed using an optical microscope (Nikon Eclipse Ts2, Japan). ..

    Article Title: TRPV1 manipulating polarization of M1/M2 macrophages to promote skeletal muscle regeneration
    Article Snippet: .. Macrophage subtypes (M1: CD86-positive and F4/80-positive; M2: CD206-positive and F4/80-positive) were counted independently by two pathologists in five randomly selected non-overlapping fields per section at 400× magnification using a fluorescence microscopy (ECLIPSE Ti2, Nikon, Japan). .. For cytological immunofluorescence, cells were fixed in 4% paraformaldehyde, permeabilized with 0.5% Triton X-100 for 15 min. After blocking with 5% BSA, the slides were incubated with the primary antibody for TRPV1, CD86, CD206, mouse anti-MYH3 (1:200, Santa Cruz Biotechnology, sc-53091), rabbit anti-IL-6 polyclonal antibody (1:200, Proteintech, #21865-1-AP), and rabbit anti-Arginase-1 polyclonal antibody (1:200, Proteintech, #16001-1-AP).

    Article Title: Synergistic Potential of Microbial Consortia for the Management of Fusarium Wilt and Growth Enhancement in Strawberry (Fragaria ananassa)
    Article Snippet: Strawberry (Fragaria ananassa) is an important fruit crop that grows well in temperate and tropical climates.. Fusarium wilt is one of the major soil borne diseases of strawberry plants resulting in significant yield losses.. Due to the high residual effect of chemical fungicides and direct consumption of fruits, disease management through fungal and bacterial biocontrol agents paves the way for sustainable, eco-friendly and effective plant disease management.

    Article Title: Aquaporins in bovine sperm: Promising target for male reproductive strategy.
    Article Snippet: The search for new indicators for assessing the quality of cryopreserved semen is necessary to reduce the number of ineffective insemination procedures in cattle.. Aquaporins (AQPs) are small transmembrane proteins that facilitate the transport of water and other small solutes across the membrane.. In this study we identified and localized AQP3, AQP7, AQP8 and AQP11 in frozenthawed bull spermatozoa and investigated the relationship between their expression and specific semen quality parameters, as well as the concentrations of free amino acids (AA), biogenic amines (BA), and the composition of fatty acids (FA) in spermatozoa and seminal plasma.

    Confocal Laser Scanning Microscopy:

    Article Title: M2 macrophage-derived exosomes delivering haptoglobin and interleukin-10 plasmids for synergistic therapy of intracerebral hemorrhage
    Article Snippet: .. For CLSM observation, 100 μL ICG and M2-exo@ICG were incubated with M1 microglia for 0.25 h, 0.5 h, 1 h and 2 h. Then cells were washed, fixed, stained with DAPI and observed by CLSM (A1R+, Nikon, Japan). ..

    Article Title: M2 macrophage-derived exosomes delivering haptoglobin and interleukin-10 plasmids for synergistic therapy of intracerebral hemorrhage
    Article Snippet: .. For CLSM observation, 100 μL ICG and M2exo@ICG were incubated with M1 microglia for 0.25 h, 0.5 h, 1 h and 2 h. Then cells were washed, fixed, stained with DAPI and observed by CLSM (A1R+, Nikon, Japan). ..

    Incubation:

    Article Title: M2 macrophage-derived exosomes delivering haptoglobin and interleukin-10 plasmids for synergistic therapy of intracerebral hemorrhage
    Article Snippet: .. For CLSM observation, 100 μL ICG and M2-exo@ICG were incubated with M1 microglia for 0.25 h, 0.5 h, 1 h and 2 h. Then cells were washed, fixed, stained with DAPI and observed by CLSM (A1R+, Nikon, Japan). ..

    Article Title: M2 macrophage-derived exosomes delivering haptoglobin and interleukin-10 plasmids for synergistic therapy of intracerebral hemorrhage
    Article Snippet: .. For CLSM observation, 100 μL ICG and M2exo@ICG were incubated with M1 microglia for 0.25 h, 0.5 h, 1 h and 2 h. Then cells were washed, fixed, stained with DAPI and observed by CLSM (A1R+, Nikon, Japan). ..

    Staining:

    Article Title: M2 macrophage-derived exosomes delivering haptoglobin and interleukin-10 plasmids for synergistic therapy of intracerebral hemorrhage
    Article Snippet: .. For CLSM observation, 100 μL ICG and M2-exo@ICG were incubated with M1 microglia for 0.25 h, 0.5 h, 1 h and 2 h. Then cells were washed, fixed, stained with DAPI and observed by CLSM (A1R+, Nikon, Japan). ..

    Article Title: M2 macrophage-derived exosomes delivering haptoglobin and interleukin-10 plasmids for synergistic therapy of intracerebral hemorrhage
    Article Snippet: .. For CLSM observation, 100 μL ICG and M2exo@ICG were incubated with M1 microglia for 0.25 h, 0.5 h, 1 h and 2 h. Then cells were washed, fixed, stained with DAPI and observed by CLSM (A1R+, Nikon, Japan). ..

    Fluorescence:

    Article Title: TRPV1 manipulating polarization of M1/M2 macrophages to promote skeletal muscle regeneration
    Article Snippet: .. Macrophage subtypes (M1: CD86-positive and F4/80-positive; M2: CD206-positive and F4/80-positive) were counted independently by two pathologists in five randomly selected non-overlapping fields per section at 400× magnification using a fluorescence microscopy (ECLIPSE Ti2, Nikon, Japan). .. For cytological immunofluorescence, cells were fixed in 4% paraformaldehyde, permeabilized with 0.5% Triton X-100 for 15 min. After blocking with 5% BSA, the slides were incubated with the primary antibody for TRPV1, CD86, CD206, mouse anti-MYH3 (1:200, Santa Cruz Biotechnology, sc-53091), rabbit anti-IL-6 polyclonal antibody (1:200, Proteintech, #21865-1-AP), and rabbit anti-Arginase-1 polyclonal antibody (1:200, Proteintech, #16001-1-AP).



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    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
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    inverted eclipse ti e microscope  (Nikon)
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    Image Search Results


    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Journal: Biofilm

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    doi: 10.1016/j.bioflm.2025.100335

    Figure Lengend Snippet: Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Article Snippet: After five days of static incubation at 30 °C, three-dimensional biofilm structures were observed using an Eclipse Ti-E C2+ confocal laser scanning microscope (Nikon) equipped with CFI Plan Apo VC 20x/1.2 and CFI Plan Apo VC 60x/1.2 WI objective lenses.

    Techniques: Staining, Mutagenesis, Fluorescence, Microscopy

    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Journal: Biofilm

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    doi: 10.1016/j.bioflm.2025.100335

    Figure Lengend Snippet: Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Article Snippet: The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope.

    Techniques: Staining, Mutagenesis, Fluorescence, Microscopy