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metafluor software  (universal imaging inc)

 
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    Structured Review

    universal imaging inc metafluor software
    Metafluor Software, supplied by universal imaging inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+te2000+5+microscope/metafluor+software/us09845318-286-15-17
    Average 90 stars, based on 1 article reviews
    metafluor software - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    other:

    Article Title: CDK4 inactivation inhibits apoptosis via mitochondria-ER contact remodeling in triple-negative breast cancer.
    Article Snippet: For ratiometric probe, the fluorescence ratio was analyzed with MetaFluor 6.3 (Universal Imaging) after removing of fluorescence background.

    Article Title: P2 Receptor Antagonists Rescue Defective Heme Content in an In Vitro SLC25A38-Associated Congenital Sideroblastic Anemia Cell Model
    Article Snippet: Images were acquired with a CoolSNAP HQ CCD camera (Roper Scientific, Trenton, NJ, USA), using MetaFluor 6.1 software (Universal Imaging Corporation, Downington, PA, USA) and processed using ImageJ software (ImageJ 1.54f, National Institutes of Health, USA, website: http://imagej.org ).

    Article Title: Protease-activated receptor-2 inhibitors for the treatment of sensory neuropathy induced by a marine neurotoxic poisoning
    Article Snippet: Emission was collected through a 415DCLP dichroic mirror, by a 14-bit CCD camera (EXiBlue, Qimaging).

    Article Title: CDK4 inactivation inhibits apoptosis via mitochondria-ER contact remodeling in triple-negative breast cancer
    Article Snippet: For ratiometric probe, the fluorescence ratio was analyzed with MetaFluor 6.3 (Universal Imaging) after removing of fluorescence background.

    Microscopy:

    Article Title: Protein kinase C (PKC) inhibitor Calphostin C activates PKC in a light-dependent manner at high concentrations via the production of singlet oxygen.
    Article Snippet: Calphostin C (Cal-C) is a protein kinase C (PKC) inhibitor that binds to its C1 domain.. The aim of the present study was to elucidate the action of Cal-C in addition to PKC inhibition.. First, we confirmed that Cal-C at low concentrations (<200 nM) inhibit phorbol ester-induced PKC translocation and G-protein-coupled receptor (GPCR)-mediated PKC activation.

    Article Title: Oncogenic GNAQ/11-induced remodeling of the IP3/Calcium signaling pathway protects Uveal Melanoma against Calcium-driven cell death
    Article Snippet: .. 100 000 cells plated on 30 mm glass coverslips were loaded with 4 μM Fura-2AM for 45 min. After 3 washes in 1.8 mM Ca 2+ HBSS, changes in [Ca 2+ ]i were monitored using an IX83 (Olympus) inverted microscope-based imaging system equipped with a 40×/1.35 UApo N340 high UV light transmittance oil immersion objective (Olympus), an ORCA-Flash4.0 Digital CMOS camera (Hamamatsu Photonics), a DG-4 Ultra High Speed Wavelength Switcher (Sutter Instruments) and METAFLUOR software (Universal Imaging) for image acquisition and analysis. .. For cellular viability assessment, eGFP- or eGFP-IP3R3-transfected cells were resuspended in 1 μg/ml Propidium Iodide (PI, 11435392, Fisher Scientific).

    Software:

    Article Title: Protein kinase C (PKC) inhibitor Calphostin C activates PKC in a light-dependent manner at high concentrations via the production of singlet oxygen.
    Article Snippet: Calphostin C (Cal-C) is a protein kinase C (PKC) inhibitor that binds to its C1 domain.. The aim of the present study was to elucidate the action of Cal-C in addition to PKC inhibition.. First, we confirmed that Cal-C at low concentrations (<200 nM) inhibit phorbol ester-induced PKC translocation and G-protein-coupled receptor (GPCR)-mediated PKC activation.

    Article Title: Osteopontin Regulates AQP4 Expression by TRPV4 Activation in Müller Cells: Implications for Retinal Homeostasis.
    Article Snippet: During the intense neuronal activity in the retina, Müller cells are exposed to a hypotonic environment and activate a regulatory volume decrease (RVD) response, which depends on Aquaporin-4 (AQP4) and the calcium channel Transient Receptor Potential Vanilloid 4 (TRPV4).. It was reported that Osteopontin (OPN), a cytokine and component of the extracellular matrix (ECM), may modulate the RVD of Müller cells.. In other cell types, OPN participates in cell survival and migration, which Müller cells undergo to maintain retinal homeostasis.

    Article Title: Oncogenic GNAQ/11-induced remodeling of the IP3/Calcium signaling pathway protects Uveal Melanoma against Calcium-driven cell death
    Article Snippet: .. 100 000 cells plated on 30 mm glass coverslips were loaded with 4 μM Fura-2AM for 45 min. After 3 washes in 1.8 mM Ca 2+ HBSS, changes in [Ca 2+ ]i were monitored using an IX83 (Olympus) inverted microscope-based imaging system equipped with a 40×/1.35 UApo N340 high UV light transmittance oil immersion objective (Olympus), an ORCA-Flash4.0 Digital CMOS camera (Hamamatsu Photonics), a DG-4 Ultra High Speed Wavelength Switcher (Sutter Instruments) and METAFLUOR software (Universal Imaging) for image acquisition and analysis. .. For cellular viability assessment, eGFP- or eGFP-IP3R3-transfected cells were resuspended in 1 μg/ml Propidium Iodide (PI, 11435392, Fisher Scientific).

    Fluorescence:

    Article Title: Osteopontin Regulates AQP4 Expression by TRPV4 Activation in Müller Cells: Implications for Retinal Homeostasis.
    Article Snippet: During the intense neuronal activity in the retina, Müller cells are exposed to a hypotonic environment and activate a regulatory volume decrease (RVD) response, which depends on Aquaporin-4 (AQP4) and the calcium channel Transient Receptor Potential Vanilloid 4 (TRPV4).. It was reported that Osteopontin (OPN), a cytokine and component of the extracellular matrix (ECM), may modulate the RVD of Müller cells.. In other cell types, OPN participates in cell survival and migration, which Müller cells undergo to maintain retinal homeostasis.

    Inverted Microscopy:

    Article Title: Osteopontin Regulates AQP4 Expression by TRPV4 Activation in Müller Cells: Implications for Retinal Homeostasis.
    Article Snippet: During the intense neuronal activity in the retina, Müller cells are exposed to a hypotonic environment and activate a regulatory volume decrease (RVD) response, which depends on Aquaporin-4 (AQP4) and the calcium channel Transient Receptor Potential Vanilloid 4 (TRPV4).. It was reported that Osteopontin (OPN), a cytokine and component of the extracellular matrix (ECM), may modulate the RVD of Müller cells.. In other cell types, OPN participates in cell survival and migration, which Müller cells undergo to maintain retinal homeostasis.

    Imaging:

    Article Title: Oncogenic GNAQ/11-induced remodeling of the IP3/Calcium signaling pathway protects Uveal Melanoma against Calcium-driven cell death
    Article Snippet: .. 100 000 cells plated on 30 mm glass coverslips were loaded with 4 μM Fura-2AM for 45 min. After 3 washes in 1.8 mM Ca 2+ HBSS, changes in [Ca 2+ ]i were monitored using an IX83 (Olympus) inverted microscope-based imaging system equipped with a 40×/1.35 UApo N340 high UV light transmittance oil immersion objective (Olympus), an ORCA-Flash4.0 Digital CMOS camera (Hamamatsu Photonics), a DG-4 Ultra High Speed Wavelength Switcher (Sutter Instruments) and METAFLUOR software (Universal Imaging) for image acquisition and analysis. .. For cellular viability assessment, eGFP- or eGFP-IP3R3-transfected cells were resuspended in 1 μg/ml Propidium Iodide (PI, 11435392, Fisher Scientific).



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    Image Search Results


    (A) Representative epifluorescence images (20×) of L4 animals expressing Pins-3::GFP under basal conditions (20 °C on Nematode Growth Media plates seeded with OP50), under oxidative stress (1 mM FeSO 4, 2 h) or heat stress (30 °C, 6 h) and in the presence of exogenous tyramine (15 mM). Scale bar 50 µm. (B) Corresponding quantification of fluorescence levels per worm. Scatter dot plot with relative expression of Pins-3::GFP normalized to basal conditions of each independent experiment. Line at the median. n = 50−200 animals per condition (distributed across 3–4 independent experiments). One-way ANOVA and Dunn’s post hoc test vs. basal were used. * p < 0.05, **** p < 0.0001. (C) Log 2 fold-changes in ins-3 transcript levels in animals exposed to oxidative stress (1 mM FeSO 4 , 2 h) or heat stress (30 °C, 6 h) and in the presence of exogenous Tyramine (15 mM). Negative and positive values indicate down- and up-regulation of ins-3 , respectively. Fold change was calculated as ΔCt basal conditions/ΔCt test conditions. Results are shown as mean ± s.e.m. n = 4 independent experiments. The data underlying this figure can be found at https://osf.io/wfgvs/ .

    Journal: PLOS Biology

    Article Title: The neurohormone tyramine stimulates the secretion of an insulin-like peptide from the Caenorhabditis elegans intestine to modulate the systemic stress response

    doi: 10.1371/journal.pbio.3002997

    Figure Lengend Snippet: (A) Representative epifluorescence images (20×) of L4 animals expressing Pins-3::GFP under basal conditions (20 °C on Nematode Growth Media plates seeded with OP50), under oxidative stress (1 mM FeSO 4, 2 h) or heat stress (30 °C, 6 h) and in the presence of exogenous tyramine (15 mM). Scale bar 50 µm. (B) Corresponding quantification of fluorescence levels per worm. Scatter dot plot with relative expression of Pins-3::GFP normalized to basal conditions of each independent experiment. Line at the median. n = 50−200 animals per condition (distributed across 3–4 independent experiments). One-way ANOVA and Dunn’s post hoc test vs. basal were used. * p < 0.05, **** p < 0.0001. (C) Log 2 fold-changes in ins-3 transcript levels in animals exposed to oxidative stress (1 mM FeSO 4 , 2 h) or heat stress (30 °C, 6 h) and in the presence of exogenous Tyramine (15 mM). Negative and positive values indicate down- and up-regulation of ins-3 , respectively. Fold change was calculated as ΔCt basal conditions/ΔCt test conditions. Results are shown as mean ± s.e.m. n = 4 independent experiments. The data underlying this figure can be found at https://osf.io/wfgvs/ .

    Article Snippet: For ins-3 and ins-4 expression levels analysis, animals containing the corresponding transcriptional GFP reporter ( Pins-3::GFP and Pins-4:GFP , respectively) were imaged using an epifluorescence microscope (Nikon Eclipse TE2000-5) coupled to a CCD camera (Nikon DS-Qi2) with 20× objective.

    Techniques: Expressing, Fluorescence

    Representative epifluorescence images (40×) of young not gravid adults expressing Pges-1::INS-3::VENUS in wild-type and tyra-3 null mutant background, in the absence or presence of exogenous tyramine (15 mM). Scale bar 25 µm. Fluorescence is observed in the two posterior coelomocytes of worms (inset). Right. Corresponding quantification of fluorescence intensity on the coelomocytes (normalized to wild-type animals without tyramine exposure). Line at the median. n = 35–40 animals per condition distributed across three independent experiments. For conditions with tyramine, a two-tailed Student’s t test vs. the same strain without tyramine was used. * p < 0.05. The data underlying this figure can be found at https://osf.io/wfgvs/ .

    Journal: PLOS Biology

    Article Title: The neurohormone tyramine stimulates the secretion of an insulin-like peptide from the Caenorhabditis elegans intestine to modulate the systemic stress response

    doi: 10.1371/journal.pbio.3002997

    Figure Lengend Snippet: Representative epifluorescence images (40×) of young not gravid adults expressing Pges-1::INS-3::VENUS in wild-type and tyra-3 null mutant background, in the absence or presence of exogenous tyramine (15 mM). Scale bar 25 µm. Fluorescence is observed in the two posterior coelomocytes of worms (inset). Right. Corresponding quantification of fluorescence intensity on the coelomocytes (normalized to wild-type animals without tyramine exposure). Line at the median. n = 35–40 animals per condition distributed across three independent experiments. For conditions with tyramine, a two-tailed Student’s t test vs. the same strain without tyramine was used. * p < 0.05. The data underlying this figure can be found at https://osf.io/wfgvs/ .

    Article Snippet: For ins-3 and ins-4 expression levels analysis, animals containing the corresponding transcriptional GFP reporter ( Pins-3::GFP and Pins-4:GFP , respectively) were imaged using an epifluorescence microscope (Nikon Eclipse TE2000-5) coupled to a CCD camera (Nikon DS-Qi2) with 20× objective.

    Techniques: Expressing, Mutagenesis, Fluorescence, Two Tailed Test