inverted microscope (Nikon)
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Nikon
inverted microscope
Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 14830 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+te2000+5+microscope/Inverted+Microscopes/pmc10785265-180-6-8
Average 99 stars, based on 14830 article reviews
Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 14830 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+te2000+5+microscope/Inverted+Microscopes/pmc10785265-180-6-8
Average 99 stars, based on 14830 article reviews
inverted microscope - by Bioz Stars,
2026-11
99/100 stars
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Immunofluorescence:Article Title: Fejerin promotes autophagy via the PINK1-Parkin pathway and is protective against Parkinson’s disease pathology Article Snippet: The subsequent day, following a PBS wash, the cells were pairedwith suitably tagged secondary antibodies(1:100, BA1127orBA1126, Boster,Wuhan, China) and kept in the dark at 37 °C for 1 h. Finally, the coverslips were secured onto glass slides using an anti-fade mounting medium laced with DAPI (Abcam, Cambridge, UK) to highlight the cell nuclei. .. Finally, immunofluorescence snapshots were taken, and fluorescence intensity was measured with both a Fluorescence:Article Title: Fejerin promotes autophagy via the PINK1-Parkin pathway and is protective against Parkinson’s disease pathology Article Snippet: The subsequent day, following a PBS wash, the cells were pairedwith suitably tagged secondary antibodies(1:100, BA1127orBA1126, Boster,Wuhan, China) and kept in the dark at 37 °C for 1 h. Finally, the coverslips were secured onto glass slides using an anti-fade mounting medium laced with DAPI (Abcam, Cambridge, UK) to highlight the cell nuclei. .. Finally, immunofluorescence snapshots were taken, and fluorescence intensity was measured with both a Article Title: 3D-printed PLCL scaffolds for breast reconstructive tissue engineering: a pre-clinical study in a rabbit model Article Snippet: At day 7 of differentiation, cells were labelled for 20 min at 37 °C with two fluorescent dyes, 1 μg/ml each: Hoechst 33,342 (Invitrogen—H3570; Thermofisher) and BODIPY 493/503 (Invitrogen—D3922; Thermofisher). .. Following two PBS washes, visualization and quantification were carried out by fluorescence microscopy using a Nikon Article Title: Mechanism of luteolin in treating chronic heart failure by regulating HIF-1 signaling pathway-mediated mitochondrial damage to inhibit cardiomyocyte apoptosis Article Snippet: The next day, a fluorophore-conjugated secondary antibody (Servicebio, Wuhan, China) and DAPI nuclear counterstain (Servicebio, Wuhan, China) were added sequentially in the dark. .. Fluorescence images were captured using an Article Title: Natural product-like phenanthroquinolizidines: asymmetric synthesis and anti-glioblastoma activity evaluation. Article Snippet: Phenanthroquinolizidine alkaloids exhibit exceptional potency and broad-spectrum antitumor activities against various human malignancies.. Here, utilizing a ring-expansion strategy based on phenanthroindolizidines, we report the substrate-controlled asymmetric synthesis of a pair of novel enantiomeric natural product-like phenanthroquinolizidines, S-CAT6 and R-CAT6.. The synthesis employs (L)or (D)-2-aminoadipic acid dimethyl ester as the chiral pool source, streamlining the sequence through a one-pot iodination/nucleophilic substitution, followed by E-ring cyclization, Friedel-Crafts acylation for D-ring annulation, and terminal deoxygenation. Article Title: Mechanistic insights into ultraviolet-crosslinked sulfonated hyaluronic acid microspheres for stable biointerfaces on biodegradable cerebrovascular magnesium implants Article Snippet: .. Optical microscopy analysis: All samles were examined using an Article Title: M2 Macrophage-Derived Small Extracellular Vesicle miR-106a-Loaded QCG Hydrogel Targets the RNF128-LXR Pathway to Reprogram Chondrocyte Cholesterol Metabolism for Osteoarthritis Therapy. 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Article Title: A smart magneto-responsive ternary 3D platform for actively steering stereoscopic infiltration and chronological osteogenic differentiation of BMSCs. Article Snippet: The limited effective infiltration of cells into the interior of scaffolds remains a critical bottleneck limiting the therapeutic application of bone tissue engineering.. To address this challenge, this study developed a physically self-assembled ternary smart magneto-responsive composite platform (MGO/CS) based on chitosan, graphene oxide, and superparamagnetic iron oxide nanoparticles (SPIONs).. 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Following two PBS washes, visualization and quantification were carried out by fluorescence microscopy using a Nikon Analysis:Article Title: 3D-printed PLCL scaffolds for breast reconstructive tissue engineering: a pre-clinical study in a rabbit model Article Snippet: At day 7 of differentiation, cells were labelled for 20 min at 37 °C with two fluorescent dyes, 1 μg/ml each: Hoechst 33,342 (Invitrogen—H3570; Thermofisher) and BODIPY 493/503 (Invitrogen—D3922; Thermofisher). .. Following two PBS washes, visualization and quantification were carried out by fluorescence microscopy using a Nikon Article Title: Mechanistic insights into ultraviolet-crosslinked sulfonated hyaluronic acid microspheres for stable biointerfaces on biodegradable cerebrovascular magnesium implants Article Snippet: .. Optical microscopy analysis: All samles were examined using an Construct:Article Title: A smart magneto-responsive ternary 3D platform for actively steering stereoscopic infiltration and chronological osteogenic differentiation of BMSCs. Article Snippet: The limited effective infiltration of cells into the interior of scaffolds remains a critical bottleneck limiting the therapeutic application of bone tissue engineering.. To address this challenge, this study developed a physically self-assembled ternary smart magneto-responsive composite platform (MGO/CS) based on chitosan, graphene oxide, and superparamagnetic iron oxide nanoparticles (SPIONs).. 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To address this challenge, this study developed a physically self-assembled ternary smart magneto-responsive composite platform (MGO/CS) based on chitosan, graphene oxide, and superparamagnetic iron oxide nanoparticles (SPIONs).. Although the incorporation of SPIONs reduced the mean pore size of the scaffold from 306.64 ± 52.25 μm to 185.39 ± 28.32 μm (p < 0.05), this smart platform, driven by a 3 mT weak static magnetic field (SMF), successfully mitigated the dependence of the threedimensional colonization of stem cells on pore size, thereby partially overcoming the constraints imposed by conventional geometric topography on deep cell infiltration. Article Title: Allogeneic Human Bone Marrow‐Derived Mesenchymal Stem Cells Accelerate Diabetic Wound Healing via Inflammation Suppression and Angiogenesis Promotion: A Preclinical Study Article Snippet: After 16–18 h of incubation, HUVECs migrated to the bottom surface of the transwell membranes and were fixed with 4% Paraformaldehyde (PFA; ChemCruz Biochemicals, Netherlands) for 1 h at room temperature, followed by cell staining with 1% crystal violet dye. .. The remaining HUVECs in the upper chamber were scraped out by the swabs, and stained cells were captured by Nikon |
