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eclipse e800 optical microscope  (Nikon)


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    Structured Review

    Nikon eclipse e800 optical microscope
    Eclipse E800 Optical Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 60152 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+e800+microscope/Objectives/pmc12756182-284-7-6
    Average 99 stars, based on 60152 article reviews
    eclipse e800 optical microscope - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Live Cell Imaging:

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine
    Article Snippet: The enteroids were imaged using a Zeiss LSM800 confocal microscope equipped with a 20x objective and a 40x immersion oil objective. .. Live-cell imaging was performed on a custom-built microscope based on an Eclipse Ti2 body (Nikon), using a 10x/0.45 Plan Apo Lambda air objective (Nikon), and a back-lit sCMOS camera with a physical pixel size of 11 μm (Prime 95B, Photometrics), and a final image pixel size of 1.12 μm. ..

    Microscopy:

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine
    Article Snippet: The enteroids were imaged using a Zeiss LSM800 confocal microscope equipped with a 20x objective and a 40x immersion oil objective. .. Live-cell imaging was performed on a custom-built microscope based on an Eclipse Ti2 body (Nikon), using a 10x/0.45 Plan Apo Lambda air objective (Nikon), and a back-lit sCMOS camera with a physical pixel size of 11 μm (Prime 95B, Photometrics), and a final image pixel size of 1.12 μm. ..

    Article Title: Establishment of a Three-Dimensional In Vitro Model of Taste Bud Cells for Fat Taste Investigation.
    Article Snippet: .. Fluorescence was monitored using a Nikon microscope (TiU) equipped with an EM-CCD (Luca-S) camera for real-time recording of digital images and an S-fluor 40× oil immersion objective (Nikon, Tokyo, Japan). ..

    Article Title: Phasic metabolic remodeling coordinates cardiac myofibroblast differentiation and function.
    Article Snippet: The coverslips were washed 3× with DPBS, they were then mounted onto glass slides using Prolong Glass Antifade Mountant (Invitrogen P36982) and allowed to cure for 1 d at room temperature in the dark. .. Cells were imaged using Nikon A1 laser scanning confocal microscope with a Nikon Plan Apo 60×1.4 oil immersion objective. .. Signals from mitochondria, the F-actin cytoskeleton, and nuclei were excited with 561, 488, and 405 nm lasers, respectively, and emissions were collected using 595/50, 525/50, and 450/50 nm band-pass filters, respectively.

    Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12
    Article Snippet: .. Two weeks later, single cell clones were identified by visual inspection with a 4 x /0.10 objective using a Nikon ECLIPSE Ts2 microscope (Nikon, Tokyo, Japan) and expanded. ..

    Article Title: PINK1-mediated mitophagy supports lung cancer initiation and limits the efficacy of KRAS -targeted therapy
    Article Snippet: After three additional washes with PBS, the samples were mounted with Fluoromount-G (Life Technologies, 00-4958-02). .. Confocal images were acquired using a Nikon A1R confocal microscope equipped with a Nikon Plan Fluor HN2 60× oil immersion objective. ..

    Article Title: Mitofusin Activation Delays Aging in Caenorhabditis elegans .
    Article Snippet: .. Confocal images were obtained on a Nikon Eclipse Ti2 confocal microscope using the Nikon CGI Plan Apo VC 60x A/1.20 WI water immersion objective (Nikon Corporation, Tokyo, Japan) [32]. ..

    Article Title: In vivo virus-free lineage tracing and live imaging reveal that NeuroD1 does not reprogram microglia into neurons
    Article Snippet: .. Confocal images of the fluorescent samples were acquired via ×20 (NA 0.75), ×40 (NA 1.30), or ×60 (NA 1.40) objectives of a Nikon A2 confocal microscope. ..

    Fluorescence:

    Article Title: Establishment of a Three-Dimensional In Vitro Model of Taste Bud Cells for Fat Taste Investigation.
    Article Snippet: .. Fluorescence was monitored using a Nikon microscope (TiU) equipped with an EM-CCD (Luca-S) camera for real-time recording of digital images and an S-fluor 40× oil immersion objective (Nikon, Tokyo, Japan). ..

    Electron Microscopy:

    Article Title: Establishment of a Three-Dimensional In Vitro Model of Taste Bud Cells for Fat Taste Investigation.
    Article Snippet: .. Fluorescence was monitored using a Nikon microscope (TiU) equipped with an EM-CCD (Luca-S) camera for real-time recording of digital images and an S-fluor 40× oil immersion objective (Nikon, Tokyo, Japan). ..

    Immunohistochemical staining:

    Article Title: Spatial Remodeling of the Immune Microenvironment During Laryngeal Carcinogenesis Reveals Prognostic Immune Patterns in Laryngeal Squamous Cell Carcinoma.
    Article Snippet: .. Following immunohistochemical staining, all TMA slides were digitized using a PreciPoint Fritz Histo Scanner (PreciPoint GmbH, Garching, Germany) equipped with a Nikon CFI Plan Apochromat 20× objective (Nikon Instruments Inc., Tokyo, Japan) to enable standardized digital pathological evaluation. .. Immunohistochemical evaluation was performed according to the recommendations of the International Immuno-Oncology Biomarker Working Group for the assessment of tumor-infiltrating lymphocytes (TILs) in solid tumors [32], with minor adaptations for TMA-based analysis.

    Staining:

    Article Title: Spatial Remodeling of the Immune Microenvironment During Laryngeal Carcinogenesis Reveals Prognostic Immune Patterns in Laryngeal Squamous Cell Carcinoma.
    Article Snippet: .. Following immunohistochemical staining, all TMA slides were digitized using a PreciPoint Fritz Histo Scanner (PreciPoint GmbH, Garching, Germany) equipped with a Nikon CFI Plan Apochromat 20× objective (Nikon Instruments Inc., Tokyo, Japan) to enable standardized digital pathological evaluation. .. Immunohistochemical evaluation was performed according to the recommendations of the International Immuno-Oncology Biomarker Working Group for the assessment of tumor-infiltrating lymphocytes (TILs) in solid tumors [32], with minor adaptations for TMA-based analysis.

    Confocal:

    Article Title: Phasic metabolic remodeling coordinates cardiac myofibroblast differentiation and function.
    Article Snippet: The coverslips were washed 3× with DPBS, they were then mounted onto glass slides using Prolong Glass Antifade Mountant (Invitrogen P36982) and allowed to cure for 1 d at room temperature in the dark. .. Cells were imaged using Nikon A1 laser scanning confocal microscope with a Nikon Plan Apo 60×1.4 oil immersion objective. .. Signals from mitochondria, the F-actin cytoskeleton, and nuclei were excited with 561, 488, and 405 nm lasers, respectively, and emissions were collected using 595/50, 525/50, and 450/50 nm band-pass filters, respectively.

    Article Title: PINK1-mediated mitophagy supports lung cancer initiation and limits the efficacy of KRAS -targeted therapy
    Article Snippet: After three additional washes with PBS, the samples were mounted with Fluoromount-G (Life Technologies, 00-4958-02). .. Confocal images were acquired using a Nikon A1R confocal microscope equipped with a Nikon Plan Fluor HN2 60× oil immersion objective. ..

    Article Title: Mitofusin Activation Delays Aging in Caenorhabditis elegans .
    Article Snippet: .. Confocal images were obtained on a Nikon Eclipse Ti2 confocal microscope using the Nikon CGI Plan Apo VC 60x A/1.20 WI water immersion objective (Nikon Corporation, Tokyo, Japan) [32]. ..

    Article Title: In vivo virus-free lineage tracing and live imaging reveal that NeuroD1 does not reprogram microglia into neurons
    Article Snippet: .. Confocal images of the fluorescent samples were acquired via ×20 (NA 0.75), ×40 (NA 1.30), or ×60 (NA 1.40) objectives of a Nikon A2 confocal microscope. ..

    Single Cell:

    Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12
    Article Snippet: .. Two weeks later, single cell clones were identified by visual inspection with a 4 x /0.10 objective using a Nikon ECLIPSE Ts2 microscope (Nikon, Tokyo, Japan) and expanded. ..

    Clone Assay:

    Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12
    Article Snippet: .. Two weeks later, single cell clones were identified by visual inspection with a 4 x /0.10 objective using a Nikon ECLIPSE Ts2 microscope (Nikon, Tokyo, Japan) and expanded. ..

    Process/Product Development:

    Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12
    Article Snippet: .. Two weeks later, single cell clones were identified by visual inspection with a 4 x /0.10 objective using a Nikon ECLIPSE Ts2 microscope (Nikon, Tokyo, Japan) and expanded. ..



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    (A) Twitching motility on 1%-agar KB medium overlaid on slides by the wild-type strain Psv NCPPB 3335 (Psv) and the Δ lysM mutants. Microscopy images were taken after 24h of incubation at 25°C with Nikon Eclipse <t>E800</t> light <t>microscope</t> (20x). The experiment was repeated three times with two repetitions per trial, and a representative image is shown per strain. (B-C) Swimming motility on 0.3%-agar KB medium. (B) Representative images of swimming halos formed by the wild-type strain Psv and the Δ lysM mutants after three days of incubation at 25°C. (C) Swimming halos are represented as the covered area (cm 2 ). Data are presented as means and standard deviation (SD) from three independent experiments, with at least four replicas per assay. Asterisks indicate statistically significant differences (ANOVA test; p < 0.0001), and “ns” indicates no significant differences between samples.
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    (A) Twitching motility on 1%-agar KB medium overlaid on slides by the wild-type strain Psv NCPPB 3335 (Psv) and the Δ lysM mutants. Microscopy images were taken after 24h of incubation at 25°C with Nikon Eclipse <t>E800</t> light <t>microscope</t> (20x). The experiment was repeated three times with two repetitions per trial, and a representative image is shown per strain. (B-C) Swimming motility on 0.3%-agar KB medium. (B) Representative images of swimming halos formed by the wild-type strain Psv and the Δ lysM mutants after three days of incubation at 25°C. (C) Swimming halos are represented as the covered area (cm 2 ). Data are presented as means and standard deviation (SD) from three independent experiments, with at least four replicas per assay. Asterisks indicate statistically significant differences (ANOVA test; p < 0.0001), and “ns” indicates no significant differences between samples.
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    Image Search Results


    (A) Twitching motility on 1%-agar KB medium overlaid on slides by the wild-type strain Psv NCPPB 3335 (Psv) and the Δ lysM mutants. Microscopy images were taken after 24h of incubation at 25°C with Nikon Eclipse E800 light microscope (20x). The experiment was repeated three times with two repetitions per trial, and a representative image is shown per strain. (B-C) Swimming motility on 0.3%-agar KB medium. (B) Representative images of swimming halos formed by the wild-type strain Psv and the Δ lysM mutants after three days of incubation at 25°C. (C) Swimming halos are represented as the covered area (cm 2 ). Data are presented as means and standard deviation (SD) from three independent experiments, with at least four replicas per assay. Asterisks indicate statistically significant differences (ANOVA test; p < 0.0001), and “ns” indicates no significant differences between samples.

    Journal: PLOS Pathogens

    Article Title: Secreted LysM proteins are required for niche competition and full virulence in Pseudomonas savastanoi during host plant infection

    doi: 10.1371/journal.ppat.1013121

    Figure Lengend Snippet: (A) Twitching motility on 1%-agar KB medium overlaid on slides by the wild-type strain Psv NCPPB 3335 (Psv) and the Δ lysM mutants. Microscopy images were taken after 24h of incubation at 25°C with Nikon Eclipse E800 light microscope (20x). The experiment was repeated three times with two repetitions per trial, and a representative image is shown per strain. (B-C) Swimming motility on 0.3%-agar KB medium. (B) Representative images of swimming halos formed by the wild-type strain Psv and the Δ lysM mutants after three days of incubation at 25°C. (C) Swimming halos are represented as the covered area (cm 2 ). Data are presented as means and standard deviation (SD) from three independent experiments, with at least four replicas per assay. Asterisks indicate statistically significant differences (ANOVA test; p < 0.0001), and “ns” indicates no significant differences between samples.

    Article Snippet: After incubation, the twitching areas were examined using a Nikon Eclipse E800 light microscope with a 20x objective lens.

    Techniques: Microscopy, Incubation, Light Microscopy, Standard Deviation