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Structured Review

Abbkine Inc ecl luminescent solution
Ecl Luminescent Solution, supplied by Abbkine Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecl+solution/ecl+luminescent+solution/pm42308795-121-6-9
Average 86 stars, based on 1 article reviews
ecl luminescent solution - by Bioz Stars, 2026-10
86/100 stars

Images

Related Articles

Membrane:

Article Title: ESM1 drives cancer angiogenesis and bevacizumab resistance via trioleate synthesis.
Article Snippet: .. After three additional 5-minute TBST washes, the membrane was treated with ECL Ultra-Sensitive Chemiluminescent Substrate (BMU102-CN, Abbkine) under dark conditions and imaged using a chemiluminescence detection system. .. The primary antibodies used are shown below: ESM1 (Abcam, ab103590), ANGPTL4 (Abcam, ab196746), FASN (Abcam, ab99359), Ubiquitin (Abcam, ab134953), NF-KB (Abcam, ab32536), IL-17 (Abcam, ab79056), Survivin (Abcam, ab134170), VEGFA (Abcam, ab155944), TWIST1 (Abcam, ab50887), P-AKT (Abcam, ab38449), Akt (Abcam, ab8805), VE-Cad (Abcam, ab205336), Flag (CST#14,793), CD99 (Abcam, ab75858), β-actin (Abcam, ab8226).

Article Title: Low-level expression of Cmyc in mature neurons: Maintaining neuronal function and preventing neurodegeneration
Article Snippet: The next day, the membranes were washed with tris-buffered saline with Tween 20 (TBST) for 10 minutes three times and then incubated with the corresponding secondary antibodies: HRP-conjugated goat anti-mouse IgG (1:10,000, ABclonal, Cat# AS003, RRID: AB_2769851) and HRP-conjugated goat anti-rabbit IgG (1:10,000, ABclonal, Cat# AS014, RRID: AB_2769854), for 2 hours at room temperature. .. After further washing with TBST for 10 minutes three times, the target protein bands on the membrane were developed using ECL chemiluminescence solution (Abbkine, Wuhan, China, Cat# BMU102), and the images were captured using a Bio-Rad imaging system (Bio-Rad, CFX Connect Real-Time System). .. The gray values of the target and reference bands were processed and analyzed using ImageJ software, with the relative protein expression quantified as the ratio of the target band’s gray value to that of the reference (GAPDH) band.

Article Title: Targeted activation of PPARG or AKT1 alleviates liver injury in mice with type 2 diabetes and sepsis by modulating inflammatory and metabolic pathways.
Article Snippet: The membrane was gently agitated in TBST buffer containing skimmed milk powder (ST677, Beyotime Biotechnology, Shanghai, China) for 2 h to completely blocking and incubated with the primary antibody solution at 4 °C overnight. .. The following morning, a suitably diluted additional antibody was prepared and combined with the cleaned membrane at ambient temperature for 2 h. The membrane was ultimately generated and imaged using an ultra-sensitive ECL chemiluminescent substrate (BMU102-CN, Abbkine Scientific, China) within a gel imaging system. .. Primary antibodies against p-AKT1 (1:1000; cst4060, CST, USA), AKT1 (AF0836), AMPK (AF6423), p-AMPK (AF3423), PPARG (AF6284) (all 1:1000; Affinity, Changzhou, China), PI3K (bs-10657R), p-PI3K (bs-6417R) (both 1:1000; Bioss, Beijing, China), as well as mTOR (66888-1-Ig) and GAPDH (66009-1-Ig) (1:1000 and 1:5000, respectively; Proteintech, Wuhan, China) were used.

Article Title: ESM1 drives cancer angiogenesis and bevacizumab resistance via trioleate synthesis
Article Snippet: .. After three additional 5-minute TBST washes, the membrane was treated with ECL Ultra-Sensitive Chemiluminescent Substrate (BMU102-CN, Abbkine) under dark conditions and imaged using a chemiluminescence detection system. .. The primary antibodies used are shown below: ESM1 (Abcam, ab103590), ANGPTL4 (Abcam, ab196746), FASN (Abcam, ab99359), Ubiquitin (Abcam, ab134953), NF-KB (Abcam, ab32536), IL-17 (Abcam, ab79056), Survivin (Abcam, ab134170), VEGFA (Abcam, ab155944), TWIST1 (Abcam, ab50887), P-AKT (Abcam, ab38449), Akt (Abcam, ab8805), VE-Cad (Abcam, ab205336), Flag (CST#14,793), CD99 (Abcam, ab75858), β-actin (Abcam, ab8226).

other:

Article Title: Berberine Attenuates Glucocorticoid-Induced Bone Loss in Mice: Associated with the Gut Microbiota–Glycerophospholipid Metabolic Axis
Article Snippet: Specific antibodies against COL1 (ab270993), BMP2 ( A27101 ) and β-actin (GB15003-100) were supplied by Abcam (Cambridge, UK), ABclonal Technology (Wuhan, China) and Servicebio (Wuhan, China).

Imaging:

Article Title: Low-level expression of Cmyc in mature neurons: Maintaining neuronal function and preventing neurodegeneration
Article Snippet: The next day, the membranes were washed with tris-buffered saline with Tween 20 (TBST) for 10 minutes three times and then incubated with the corresponding secondary antibodies: HRP-conjugated goat anti-mouse IgG (1:10,000, ABclonal, Cat# AS003, RRID: AB_2769851) and HRP-conjugated goat anti-rabbit IgG (1:10,000, ABclonal, Cat# AS014, RRID: AB_2769854), for 2 hours at room temperature. .. After further washing with TBST for 10 minutes three times, the target protein bands on the membrane were developed using ECL chemiluminescence solution (Abbkine, Wuhan, China, Cat# BMU102), and the images were captured using a Bio-Rad imaging system (Bio-Rad, CFX Connect Real-Time System). .. The gray values of the target and reference bands were processed and analyzed using ImageJ software, with the relative protein expression quantified as the ratio of the target band’s gray value to that of the reference (GAPDH) band.

Article Title: Targeted activation of PPARG or AKT1 alleviates liver injury in mice with type 2 diabetes and sepsis by modulating inflammatory and metabolic pathways.
Article Snippet: The membrane was gently agitated in TBST buffer containing skimmed milk powder (ST677, Beyotime Biotechnology, Shanghai, China) for 2 h to completely blocking and incubated with the primary antibody solution at 4 °C overnight. .. The following morning, a suitably diluted additional antibody was prepared and combined with the cleaned membrane at ambient temperature for 2 h. The membrane was ultimately generated and imaged using an ultra-sensitive ECL chemiluminescent substrate (BMU102-CN, Abbkine Scientific, China) within a gel imaging system. .. Primary antibodies against p-AKT1 (1:1000; cst4060, CST, USA), AKT1 (AF0836), AMPK (AF6423), p-AMPK (AF3423), PPARG (AF6284) (all 1:1000; Affinity, Changzhou, China), PI3K (bs-10657R), p-PI3K (bs-6417R) (both 1:1000; Bioss, Beijing, China), as well as mTOR (66888-1-Ig) and GAPDH (66009-1-Ig) (1:1000 and 1:5000, respectively; Proteintech, Wuhan, China) were used.

Article Title: Oxidative stress-driven m 5 C methylation by NSUN5 confers cisplatin resistance in lung adenocarcinoma through promoting glycolysis
Article Snippet: Equal protein loads were separated by SDS-PAGE gels (Cat# PG112, PG113, Epizyme Biotech, China) and subsequently transferred to polyvinylidene difluoride (PVDF) membranes (Cat# 03010040001, Roche, USA). .. Following overnight incubation with primary antibodies at 4 °C and washing, protein bands were detected using an enhanced chemiluminescence (ECL) kit (Cat# BMU102-CN, Abbkine, Wuhan, China) and visualized on a Tannon (Shanghai, China) imaging system. ..

Generated:

Article Title: Targeted activation of PPARG or AKT1 alleviates liver injury in mice with type 2 diabetes and sepsis by modulating inflammatory and metabolic pathways.
Article Snippet: The membrane was gently agitated in TBST buffer containing skimmed milk powder (ST677, Beyotime Biotechnology, Shanghai, China) for 2 h to completely blocking and incubated with the primary antibody solution at 4 °C overnight. .. The following morning, a suitably diluted additional antibody was prepared and combined with the cleaned membrane at ambient temperature for 2 h. The membrane was ultimately generated and imaged using an ultra-sensitive ECL chemiluminescent substrate (BMU102-CN, Abbkine Scientific, China) within a gel imaging system. .. Primary antibodies against p-AKT1 (1:1000; cst4060, CST, USA), AKT1 (AF0836), AMPK (AF6423), p-AMPK (AF3423), PPARG (AF6284) (all 1:1000; Affinity, Changzhou, China), PI3K (bs-10657R), p-PI3K (bs-6417R) (both 1:1000; Bioss, Beijing, China), as well as mTOR (66888-1-Ig) and GAPDH (66009-1-Ig) (1:1000 and 1:5000, respectively; Proteintech, Wuhan, China) were used.

Incubation:

Article Title: Oxidative stress-driven m 5 C methylation by NSUN5 confers cisplatin resistance in lung adenocarcinoma through promoting glycolysis
Article Snippet: Equal protein loads were separated by SDS-PAGE gels (Cat# PG112, PG113, Epizyme Biotech, China) and subsequently transferred to polyvinylidene difluoride (PVDF) membranes (Cat# 03010040001, Roche, USA). .. Following overnight incubation with primary antibodies at 4 °C and washing, protein bands were detected using an enhanced chemiluminescence (ECL) kit (Cat# BMU102-CN, Abbkine, Wuhan, China) and visualized on a Tannon (Shanghai, China) imaging system. ..

Article Title: α-Linolenic acid alleviates ovalbumin-induced allergic rhinitis in mice and modulates GPR120-NF-κB signaling.
Article Snippet: .. AR TIC LE IN PR ES S ARTICLE IN PRESS incubated with the corresponding secondary antibody (Cat. No. AS014, 1:8000, ABclonal Biological Technology) at room temperature for 2 h. After thorough washing, the signal was developed using the Ultra-Sensitive ECL Chemiluminescence Reagent Kit (Cat. No. BMU102, Abbkine Scientific Co., Ltd.). .. Protein optical density was measured using ImageJ-win64 software.



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