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solution  (Cytiva Europe)


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    Structured Review

    Cytiva Europe solution
    Solution, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 98/100, based on 9908 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecl+solution/Amersham+ECL+Prime+Western+blot+detection+reagent/pm42214744-146-11-12
    Average 98 stars, based on 9908 article reviews
    solution - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    Transcranial Magnetic Stimulation:

    Article Title: Stemness overcomes IFNγ-induced antitumour exhaustion in human macrophages
    Article Snippet: Lysates were homogenized using QIAshredder (79656, Qiagen), denatured, and loaded onto SDS-polyacrylamide gels for electrophoresis (SDS-PAGE), followed by semi-dry blotting. .. Blots were blocked with 5% non-fat dry milk (68514-61-4, Biomol) in TBS-T (J77500.K2, ThermoFisher), and incubated with anti-MAFB (HPA005653, Sigma) or anti-MAF antibodies (HPA028289, Sigma), then with a secondary antibody, anti-rabbit IgG-HRP (12-348, Sigma), followed by incubation with ECL TM Prime Western Blot detection reagent (RPN2232, GE Healthcare Amersham TM ). .. Total RNA was isolated from iPSCs or differentiation stages using the RNeasy Mini Kit (74104, Qiagen), or RNeasy Micro Kit (74004, Qiagen), followed by cDNA synthesis using the High-Capacity Reverse Transcription Kit (4368814, Applied Biosystems).

    Incubation:

    Article Title: Stemness overcomes IFNγ-induced antitumour exhaustion in human macrophages
    Article Snippet: Lysates were homogenized using QIAshredder (79656, Qiagen), denatured, and loaded onto SDS-polyacrylamide gels for electrophoresis (SDS-PAGE), followed by semi-dry blotting. .. Blots were blocked with 5% non-fat dry milk (68514-61-4, Biomol) in TBS-T (J77500.K2, ThermoFisher), and incubated with anti-MAFB (HPA005653, Sigma) or anti-MAF antibodies (HPA028289, Sigma), then with a secondary antibody, anti-rabbit IgG-HRP (12-348, Sigma), followed by incubation with ECL TM Prime Western Blot detection reagent (RPN2232, GE Healthcare Amersham TM ). .. Total RNA was isolated from iPSCs or differentiation stages using the RNeasy Mini Kit (74104, Qiagen), or RNeasy Micro Kit (74004, Qiagen), followed by cDNA synthesis using the High-Capacity Reverse Transcription Kit (4368814, Applied Biosystems).

    Western Blot:

    Article Title: Stemness overcomes IFNγ-induced antitumour exhaustion in human macrophages
    Article Snippet: Lysates were homogenized using QIAshredder (79656, Qiagen), denatured, and loaded onto SDS-polyacrylamide gels for electrophoresis (SDS-PAGE), followed by semi-dry blotting. .. Blots were blocked with 5% non-fat dry milk (68514-61-4, Biomol) in TBS-T (J77500.K2, ThermoFisher), and incubated with anti-MAFB (HPA005653, Sigma) or anti-MAF antibodies (HPA028289, Sigma), then with a secondary antibody, anti-rabbit IgG-HRP (12-348, Sigma), followed by incubation with ECL TM Prime Western Blot detection reagent (RPN2232, GE Healthcare Amersham TM ). .. Total RNA was isolated from iPSCs or differentiation stages using the RNeasy Mini Kit (74104, Qiagen), or RNeasy Micro Kit (74004, Qiagen), followed by cDNA synthesis using the High-Capacity Reverse Transcription Kit (4368814, Applied Biosystems).

    Article Title: Reverse engineering Lewy bodies suggests α-synuclein pathology as condensatopathy
    Article Snippet: HRP-conjugated secondary antibodies used: goat anti-mouse (Sigma, A4416, 1:5000) and goat anti-rabbit (Sigma, A6154, 1:5000). .. Blots were developed using Amersham ECL Prime Western Blotting Detection Reagents (Cytiva, RPN2232) and imaged on Fusion FX device (Vilber). ..

    Article Title: TPD54 Downregulates ERK Signaling in Oral Squamous Cell Carcinoma Cells
    Article Snippet: Membranes were blocked with Tris-buffered saline (Takara Bio, Shiga, Japan) containing 0.2% skim milk (Cell Signaling Technology) for 1 h at room temperature and incubated sequentially with the appropriate primary and secondary antibodies as previously described [24]. .. Protein bands were detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare UK Ltd., Buckinghamshire, UK) and visualized using a ChemiDoc XRS Plus Imaging System (BioRad). .. Band intensities were quantified using ImageJ software (version 1.54d; NIH, Bethesda, MD, USA) and normalized to GAPDH.

    Article Title: Carbonic anhydrase VIII regulates IP3R1-dependent Ca 2+ signaling to coordinate insulin secretion and hepatic glucose production
    Article Snippet: .. Signals were detected using Amersham ECL Prime Western Blotting Detection Reagent (catalog no. RPN2232, Cytiva) and visualized using an ImageQuant LAS 4000-mini (GE Healthcare, Chicago, IL, USA). ..

    Imaging:

    Article Title: TPD54 Downregulates ERK Signaling in Oral Squamous Cell Carcinoma Cells
    Article Snippet: Membranes were blocked with Tris-buffered saline (Takara Bio, Shiga, Japan) containing 0.2% skim milk (Cell Signaling Technology) for 1 h at room temperature and incubated sequentially with the appropriate primary and secondary antibodies as previously described [24]. .. Protein bands were detected using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare UK Ltd., Buckinghamshire, UK) and visualized using a ChemiDoc XRS Plus Imaging System (BioRad). .. Band intensities were quantified using ImageJ software (version 1.54d; NIH, Bethesda, MD, USA) and normalized to GAPDH.

    Article Title: Maternal obesity accelerates cardiomyocyte maturation and programs adult heart dysfunction in offspring
    Article Snippet: Membranes were incubated with the appropriate primary antibodies (cf.), followed by HRP-conjugated secondary antibodies (Cell Signaling Technology). .. Protein bands were visualized using ECL Prime detection reagent (GE Healthcare), and images were acquired with a LAS4000 imaging system (GE Healthcare). .. Since no stable loading control protein was identified between the ND and HFD-Mat groups, target protein levels were normalized to total protein content using Ponceau Red staining (P7170, Sigma-Aldrich).



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