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Amersham Life Sciences Inc ecl select tm western blotting detection kit
Ecl Select Tm Western Blotting Detection Kit, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecl+select+kit/ecl+select/pmc13028708-87-26-33
Average 86 stars, based on 1 article reviews
ecl select tm western blotting detection kit - by Bioz Stars, 2026-11
86/100 stars

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Related Articles

Incubation:

Article Title: Cefepime Alleviates Comorbid Pain and Depression Induced by Lipopolysaccharide in Female Mice
Article Snippet: Concisely, sixty micrograms of brain tissue samples were electrophoresed onto 12% SDS polyacrylamide gel using electrophoresis unit and transferred to a nitrocellulose membrane at 100 V for 1.5 h. Following blocking, membranes were incubated overnight at 4 °C with primary antibodies against GLT-1, Iba-1, and β-actin obtained from Santa Cruz Biotechnology, Dallas, TX, USA, followed by a 1 h incubation with HRP-conjugated secondary antibodies at room temperature ( ). .. After incubation, signals were visualized using ECL Select (Amersham, Buckinghamshire, UK), and the protein bands were quantified via densitometric analysis. β-actin was used as a loading control. ..

Article Title: Cefepime Alleviates Comorbid Pain and Depression Induced by Lipopolysaccharide in Female Mice
Article Snippet: Concisely, sixty micrograms of brain tissue samples were electrophoresed onto 12% SDS polyacrylamide gel using electrophoresis unit and transferred to a nitrocellulose membrane at 100 V for 1.5 h. Following blocking, membranes were incubated overnight at 4 ◦C with primary antibodies against GLT-1, Iba-1, and β-actin obtained from Santa Cruz Biotechnology, Dallas, TX, USA, followed by a 1 h incubation with HRP-conjugated secondary antibodies at room temperature (Table 1). .. After incubation, signals were visualized using ECL Select (Amersham, Buckinghamshire, UK), and the protein bands were quantified via densitometric analysis. β-actin was used as a loading control. ..

Control:

Article Title: Cefepime Alleviates Comorbid Pain and Depression Induced by Lipopolysaccharide in Female Mice
Article Snippet: Concisely, sixty micrograms of brain tissue samples were electrophoresed onto 12% SDS polyacrylamide gel using electrophoresis unit and transferred to a nitrocellulose membrane at 100 V for 1.5 h. Following blocking, membranes were incubated overnight at 4 °C with primary antibodies against GLT-1, Iba-1, and β-actin obtained from Santa Cruz Biotechnology, Dallas, TX, USA, followed by a 1 h incubation with HRP-conjugated secondary antibodies at room temperature ( ). .. After incubation, signals were visualized using ECL Select (Amersham, Buckinghamshire, UK), and the protein bands were quantified via densitometric analysis. β-actin was used as a loading control. ..

Article Title: Cefepime Alleviates Comorbid Pain and Depression Induced by Lipopolysaccharide in Female Mice
Article Snippet: Concisely, sixty micrograms of brain tissue samples were electrophoresed onto 12% SDS polyacrylamide gel using electrophoresis unit and transferred to a nitrocellulose membrane at 100 V for 1.5 h. Following blocking, membranes were incubated overnight at 4 ◦C with primary antibodies against GLT-1, Iba-1, and β-actin obtained from Santa Cruz Biotechnology, Dallas, TX, USA, followed by a 1 h incubation with HRP-conjugated secondary antibodies at room temperature (Table 1). .. After incubation, signals were visualized using ECL Select (Amersham, Buckinghamshire, UK), and the protein bands were quantified via densitometric analysis. β-actin was used as a loading control. ..

Western Blot:

Article Title: CrpH of Bordetella pertussis , a prototypic PepSY_TM protein supporting heme-copper oxidoreductase function
Article Snippet: The recombinant protein produced in E. coli was detected using an antibody against a His6 tag (SigmaAldrich) followed with an anti-mouse–HRP (dilution 1:5,000; Promega). .. Detection was performed with the Amersham ECL Prime Western Blotting System using the Amersham Imager 600 (GE). ..

Article Title: Pro-Inflammatory Response of Bovine Lung Explant Induced by Mycoplasma mycoides subsp. mycoides
Article Snippet: After washing three times with PBST and once with 10% skimmed milk in PBST for 10 min at room temperature, the membranes were incubated for 1 h at room temperature with goat anti-rabbit IgG-HRP (Bio-Rad) diluted to 1:3000, or anti-mouse IgG-HRP (GE Healthcare) diluted to 1:8000 in PBST containing 2.5% skimmed milk. .. After five washes with PBST for 5 min and one final wash with PBS for 10 min, the membranes were developed for chemiluminescence detection with the ECL Select TM Western blotting detection kit (Amersham TM , GE Healthcare, Little Chalmont, UK). .. Images were acquired using ChemiDoc MP (Bio-Rad) and Image Lab Software, version 4.0.1 (Bio-Rad).

Article Title: Downregulation of motility during stress requires stressosome input in Listeria monocytogenes strain EGD-e.
Article Snippet: Secondary anti-rabbit horseradish peroxidase-conjugated antibody (as09602; Agrisera, Vännäs, Sweden) was incubated (1:10,000 in PBS-T) for 1 h at room temperature. .. Membranes were washed for 4 × 5 min in PBS-T and ECL Prime Western Blotting System (Amersham/GE Healthcare) was used for detection according to the manufacturer. ..

Article Title: IFI207 promotes antiviral responses by modulating STING ubiquitination and degradation
Article Snippet: Proteins were detected with primary antibodies against STING (Cell Signaling Technology [CST] D1V5L), phospho-STING (CST D8F4W), phospho-TBK1 (CST D52C2), phospho-IRF3 (CST 4D4G), HA-tag (CST C29F4), FLAG-tag (Sigma F1804), V5-tag (Invitrogen 46-0705) or α-Tubulin (Sigma T6199). .. HRP-conjugated antibodies (CST anti-rabbit or anti-mouse HRP IgG,7074 and 7076, respectively) were used for detection with Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific, 322209), ECL Prime Western Blotting Reagent (GE Healthcare Amersham, RPN2232) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34095). .. RNA was isolated by RNeasy Mini Kit (QIAGEN 74106) with on-column DNase digestion using RNase-Free DNase Set (QIAGEN 79254).

Article Title: A new family of TonB-dependent copper transporters linked to respiratory oxidase function
Article Snippet: .. Detection was performed with the Amersham ECL Prime Western Blotting System using the Amersham Imager 600 (GE). ..

Sensitivity:

Article Title: IFI207 promotes antiviral responses by modulating STING ubiquitination and degradation
Article Snippet: Proteins were detected with primary antibodies against STING (Cell Signaling Technology [CST] D1V5L), phospho-STING (CST D8F4W), phospho-TBK1 (CST D52C2), phospho-IRF3 (CST 4D4G), HA-tag (CST C29F4), FLAG-tag (Sigma F1804), V5-tag (Invitrogen 46-0705) or α-Tubulin (Sigma T6199). .. HRP-conjugated antibodies (CST anti-rabbit or anti-mouse HRP IgG,7074 and 7076, respectively) were used for detection with Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific, 322209), ECL Prime Western Blotting Reagent (GE Healthcare Amersham, RPN2232) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34095). .. RNA was isolated by RNeasy Mini Kit (QIAGEN 74106) with on-column DNase digestion using RNase-Free DNase Set (QIAGEN 79254).



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