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NEN Life Science ecl plustm western blotting detection system
Ecl Plustm Western Blotting Detection System, supplied by NEN Life Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecl+plustm/ecl+western+blotting+detection+system/pm39074903-249-7-13
Average 90 stars, based on 1 article reviews
ecl plustm western blotting detection system - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Aminoguanidine exhibits an inhibitory effect on β‑amyloid‑induced damage in F98 glioma cells.
Article Snippet: The ECL-western blot detection kit (NEN, MA) was used for the visualization of immunoreactivity.

Incubation:

Article Title: Carbohydrate-based peritoneal dialysis fluid comprising glutamine residue
Article Snippet: Membranes were incubated with the HSP-72 antibody (SPA 810, Stressgen, B.C., Canada). .. Detection was accomplished by incubation with secondary, peroxidase-coupled antibodies (Sigma, USA) and enhanced chemiluminescence (ECL) using ECL Western blotting analysis system and protocols (Renaissance, NEN-Life Science Products, Boston, Mass., USA). .. Densitometry was performed with image analysis software (Molecular Analyst software, Bio-Rad, USA).

Article Title: Peritoneal dialysis fluid comprising a GSK-3 inhibitor
Article Snippet: .. Membranes are incubated with the respective primary antibody (HSP-72, p-GSK-3β) for 16 h. Detection is accomplished by incubation with secondary, peroxidase-coupled antibodies (anti-mouse or anti-rabbit IgG, both Dako Cytomation, Calif., USA) and enhanced chemiluminescence (ECL) using the ECL western blotting analysis system (Renaissance, NEN-Life Science Products, Boston, Mass., USA). .. Data Analysis All statistical analyses were performed using Sigmaplot 11.0 software (Systat Software GmbH, Erkrath, Germany).

Western Blot:

Article Title: Carbohydrate-based peritoneal dialysis fluid comprising glutamine residue
Article Snippet: Membranes were incubated with the HSP-72 antibody (SPA 810, Stressgen, B.C., Canada). .. Detection was accomplished by incubation with secondary, peroxidase-coupled antibodies (Sigma, USA) and enhanced chemiluminescence (ECL) using ECL Western blotting analysis system and protocols (Renaissance, NEN-Life Science Products, Boston, Mass., USA). .. Densitometry was performed with image analysis software (Molecular Analyst software, Bio-Rad, USA).

Article Title: Simultaneous measurement of ERK, p38, and JNK MAP kinase cascades in vascular smooth muscle cells
Article Snippet: .. Immune complexes were detected by the ECL Western blotting detection method (Renaissance Plus, NEN Life Sciences, Boston, MA) according to the manufacturer’s instructions and visualized using Kodak BioMax ML film. ..

Article Title: Quantitative assessment of the nanoanatomy of the contractile vacuole complex in Trypanosoma cruzi
Article Snippet: For TcVps34, the transferred membranes were incubated for 2 h with a 1:4,000 dilution of rat anti-HA high affinity monoclonal antibody (Roche Applied Science), and detection was carried out by incubating with a 1:5,000 dilution of a goat anti-rabbit or goat anti-rat conjugated to peroxidase (Sigma-Aldrich). .. The membranes were then developed with the ECL PlusTM Western Blotting Detection System (NEN Life Science Products). .. To control for sample loading, the blots were also probed with a monoclonal mouse anti–β-tubulin antibody (1:400 dilution; Chemicon International).

Article Title: Phosphatidylinositol kinase activities in Trypanosoma cruzi epimastigotes.
Article Snippet: Accepted Manuscript Title: Phosphatidylinositol kinase activities in Trypanosoma cruzi epimastigotes Author: Alba Marina Gimenez Marı́a Celeste Gesumarı́a Alejandra C. Schoijet Guillermo D. Alonso Mirtha M. Flawiá Graciela E. Racagni Estela E. Machado PII: S0166-6851(15)30033-5 DOI: http://dx.doi.org/doi:10.1016/j.molbiopara.2015.10.002 Reference: MOLBIO 10931 To appear in: Molecular & Biochemical Parasitology Received date: 10-4-2015 Revised date: 9-10-2015 Accepted date: 13-10-2015 Please cite this article as: Gimenez Alba Marina, Gesumaría María Celeste, Schoijet Alejandra C, Alonso Guillermo D, Flawiá Mirtha M, Racagni Graciela E, Machado Estela E.Phosphatidylinositol kinase activities in Trypanosoma cruzi epimastigotes.Molecular and Biochemical Parasitology http://dx.doi.org/10.1016/j.molbiopara.2015.10.002 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Progesterone regulation of tissue factor depends on MEK1/2 activation and requires the proline-rich site on progesterone receptor.
Article Snippet: To characterize the molecular mechanism and map the response element used by progesterone (P) to upregulate tissue factor (TF) in breast cancer cells.. TF expression and mRNA levels were analyzed in breast cancer ZR-75 and T47D cells, using Western blot and real-time PCR, respectively.. Mapping of the TF promoter was performed using luciferase vectors.

Article Title: Peritoneal dialysis fluid comprising a GSK-3 inhibitor
Article Snippet: .. Membranes are incubated with the respective primary antibody (HSP-72, p-GSK-3β) for 16 h. Detection is accomplished by incubation with secondary, peroxidase-coupled antibodies (anti-mouse or anti-rabbit IgG, both Dako Cytomation, Calif., USA) and enhanced chemiluminescence (ECL) using the ECL western blotting analysis system (Renaissance, NEN-Life Science Products, Boston, Mass., USA). .. Data Analysis All statistical analyses were performed using Sigmaplot 11.0 software (Systat Software GmbH, Erkrath, Germany).

Article Title: Quantitative assessment of the nanoanatomy of the contractile vacuole complex in Trypanosoma cruzi .
Article Snippet: For TcVps34, the transferred membranes were incubated for 2 h with a 1:4,000 dilution of rat anti-HA high affinity monoclonal antibody (Roche Applied Science), and detection was carried out by incubating with a 1:5,000 dilution of a goat anti-rabbit or goat antirat conjugated to peroxidase (Sigma-Aldrich). .. The membranes were then developed with the ECL PlusTM Western Blotting Detection System (NEN Life Science Products). .. To control for sample loading, the blots were also probed with a monoclonal mouse anti–β-tubulin antibody (1:400 dilution; Chemicon International).



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