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protein bands  (MedChemExpress)


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    Structured Review

    MedChemExpress protein bands
    Protein Bands, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecl+kit/High+Sensitivity+ECL+Kit/pm42551197-155-2-14
    Average 95 stars, based on 45 article reviews
    protein bands - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    other:

    Article Title: Cuscuta chinensis Lam. extract alleviates ovariectomy-induced osteoporosis by inhibiting VCP-mediated METTL3 protein degradation.
    Article Snippet: After separation by 12% SDS-PAGE, protein samples were transferred onto PVDF membranes (FFP39, Beyotime).

    Blocking Assay:

    Article Title: Basp1 Intensifies Neutrophil Migration and NETs Formation in Traumatic Brain Injury.
    Article Snippet: Brain abundant membrane attached signal protein 1 (Basp1), a member of the neuronal growth-associated protein family, was originally identified for its high expression in the central nervous system.. Recent evidence suggests its involvement in peripheral diseases through modulation of myeloid cell function; however, the molecular mechanisms remain poorly defined.. In this study, by re-analyzing single-cell RNA sequencing data from peripheral blood of TBI patients and validating results in a mouse TBI model via flow cytometry, we demonstrated that Basp1 was specifically upregulated in neutrophils after injury.

    Incubation:

    Article Title: Basp1 Intensifies Neutrophil Migration and NETs Formation in Traumatic Brain Injury.
    Article Snippet: Brain abundant membrane attached signal protein 1 (Basp1), a member of the neuronal growth-associated protein family, was originally identified for its high expression in the central nervous system.. Recent evidence suggests its involvement in peripheral diseases through modulation of myeloid cell function; however, the molecular mechanisms remain poorly defined.. In this study, by re-analyzing single-cell RNA sequencing data from peripheral blood of TBI patients and validating results in a mouse TBI model via flow cytometry, we demonstrated that Basp1 was specifically upregulated in neutrophils after injury.

    Article Title: Tp53/HIF1α co-synergistically promotes CRMP4 expression via promoter-proximal interactions in colorectal cancer
    Article Snippet: The samples were resolved by 8-12% SDS-PAGE and transferred to nitrocellulose membranes (Cat. No. F619511, Sangon Biotech). .. To show the level changes of protein CRMP4, p53, HIF1α, HIF1β, Flag and β-Actin, aliquots of proteins were immunoblotted with primary antibodies against CRMP4 (1: 2,000, Cat. No. 13661-1-AP, Proteintech, AR TI CL E IN P RE SS Wuhan, China), p53 (1: 2,000), HIF1α (1: 2,000), HIF1β (1: 2,000, Cat. No. #5537, Proteintech), Flag (1: 5,000, Cat. No. 20543-1-AP), β-Actin (1: 5,000, Cat. No. 81115-1-RR, Proteintech) at 4°C overnight, and then incubated with HRP-conjugated Goat Anti-Rabbit IgG (1: 5,000, Cat. No. SA00001-2, Proteintech) or Anti-Mouse IgG (1: 5,000, Cat. No. SA00001-1) at room temperature for 1 h. Membranes were developed using the High Sensitivity ECL Kit (Cat. No. HY-K2005, MedChemExpress). .. Western blot images were scanned using the ChemiScope 6000EXP System (Clinx Science Instruments Co., Ltd, China).

    Control:

    Article Title: Basp1 Intensifies Neutrophil Migration and NETs Formation in Traumatic Brain Injury.
    Article Snippet: Brain abundant membrane attached signal protein 1 (Basp1), a member of the neuronal growth-associated protein family, was originally identified for its high expression in the central nervous system.. Recent evidence suggests its involvement in peripheral diseases through modulation of myeloid cell function; however, the molecular mechanisms remain poorly defined.. In this study, by re-analyzing single-cell RNA sequencing data from peripheral blood of TBI patients and validating results in a mouse TBI model via flow cytometry, we demonstrated that Basp1 was specifically upregulated in neutrophils after injury.

    Sensitivity:

    Article Title: Tp53/HIF1α co-synergistically promotes CRMP4 expression via promoter-proximal interactions in colorectal cancer
    Article Snippet: The samples were resolved by 8-12% SDS-PAGE and transferred to nitrocellulose membranes (Cat. No. F619511, Sangon Biotech). .. To show the level changes of protein CRMP4, p53, HIF1α, HIF1β, Flag and β-Actin, aliquots of proteins were immunoblotted with primary antibodies against CRMP4 (1: 2,000, Cat. No. 13661-1-AP, Proteintech, AR TI CL E IN P RE SS Wuhan, China), p53 (1: 2,000), HIF1α (1: 2,000), HIF1β (1: 2,000, Cat. No. #5537, Proteintech), Flag (1: 5,000, Cat. No. 20543-1-AP), β-Actin (1: 5,000, Cat. No. 81115-1-RR, Proteintech) at 4°C overnight, and then incubated with HRP-conjugated Goat Anti-Rabbit IgG (1: 5,000, Cat. No. SA00001-2, Proteintech) or Anti-Mouse IgG (1: 5,000, Cat. No. SA00001-1) at room temperature for 1 h. Membranes were developed using the High Sensitivity ECL Kit (Cat. No. HY-K2005, MedChemExpress). .. Western blot images were scanned using the ChemiScope 6000EXP System (Clinx Science Instruments Co., Ltd, China).

    Article Title: Perfluorooctane sulfonate (PFOS) induces Caspase-8-mediated pyroptosis via GSDMD and GSDME to drive retinal disease: Evidence from epidemiological and experimental studies.
    Article Snippet: While perfluorooctane sulfonate (PFOS) has been epidemiologically associated with ocular disorders, its direct specific role in retinal disease and the underlying molecular mechanisms are still not well understood.. We initially examined serum PFAS levels in relation to retinal diseases among 777 participants (666 controls vs. 111 cases) from the Isomer of C8 Health Project in China.. Then, C57BL/6 mice were orally gavaged with PFOS at doses of 0.8, 8, and 80 μg/kg/day for 90 days in vivo.

    Membrane:

    Article Title: Osthole promotes cell senescence-like phenotype to enhance radiosensitivity in non-small cell lung carcinoma possibly through modulating the Sirt1/NF-κB pathway
    Article Snippet: Background Radioresistance is a major problem in non-small cell lung carcinoma (NSCLC) treatment.. Osthole is a natural coumarin with antitumor effects.. This research sought to clarify if Osthole enhances the sensitivity of NSCLC to radiotherapy and to uncover the underlying mechanisms involved.

    Analysis:

    Article Title: Perfluorooctane sulfonate (PFOS) induces Caspase-8-mediated pyroptosis via GSDMD and GSDME to drive retinal disease: Evidence from epidemiological and experimental studies.
    Article Snippet: While perfluorooctane sulfonate (PFOS) has been epidemiologically associated with ocular disorders, its direct specific role in retinal disease and the underlying molecular mechanisms are still not well understood.. We initially examined serum PFAS levels in relation to retinal diseases among 777 participants (666 controls vs. 111 cases) from the Isomer of C8 Health Project in China.. Then, C57BL/6 mice were orally gavaged with PFOS at doses of 0.8, 8, and 80 μg/kg/day for 90 days in vivo.



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