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high sensitivity ecl kit  (MedChemExpress)


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    Structured Review

    MedChemExpress high sensitivity ecl kit
    High Sensitivity Ecl Kit, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 206 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecl+kit/Ultra+High+Sensitivity+ECL+Kit/pm42477307-34-50-54
    Average 97 stars, based on 206 article reviews
    high sensitivity ecl kit - by Bioz Stars, 2026-10
    97/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: Molecular Editing Reveals a Bilobalide Chemotype that Attenuates Prolyl Endopeptidase (PREP)-Linked Neuroinflammation.
    Article Snippet: Proteins were transferred nto 0.2- μm PVDF membranes (Thermo Scientific) and blocked ith blocking buffer (ACE Biotechnology). .. Membranes were ncubated with specific primary antibodies (refer to Table S2 ) vernight at 4◦C, followed by HRP or DyLight 488-conjugated secndary antibodies (refer to Table S2 ) for 1 h at room temperature. rotein bands were visualized with the Ultra High Sensitivity ECL it (MedChemExpress, NJ, USA) for HRP-secondary antibodies nd imaged using the ChemiDoc MP Imaging System (Bioad, CA, USA). ..

    Article Title: Dexmedetomidine Activates AMPK/SIRT1 Pathway to Reduce Inflammatory Levels and Neuronal Pyroptosis to Alleviate Neonatal Hypoxic-Ischemic Brain Damage.
    Article Snippet: Neonatal hypoxic-ischemic brain damage (HIBD) is the leading cause of neonatal death and long-term neurological dysfunction.. Dexmedetomidine (DEX) has been shown to have neuroprotective effects, but its mechanisms in HIBD remain incompletely understood.. This study aimed to explore whether DEX inhibits neuroinflammation and pyroptosis via activating adenosine monophosphate-activated protein kinase (AMPK)/sirtuin 1 (SIRT1) axis, thereby reducing HIBD.

    Article Title: Down-regulation of THBS2 inhibits the malignant progression of Burkitt lymphoma by blocking the PI3K/AKT/c-MYC pathway
    Article Snippet: .. The PVDF membrane was used for membrane transfer, blocked with 5% skim milk for 2 h, and then incubated with primary antibody dilution at 4°C overnight, and then incubated with the corresponding secondary antibody dilution for 2 h. ECL kit (HY-K1005, MedChemExpress) was used for chemiluminescence detection, and gel imaging system (Geldoc Go, Bio-Rad, Shanghai, China) was used to detect protein bands. ..

    Article Title: Glutamine sequestration promotes tumor growth and immune evasion through elevation of arginine methylation in hepatocellular carcinoma
    Article Snippet: Western blot was performed using primary antibodies against: MMA(#8711,1:1,000), ADMA(#13522,1:1,000), SDMA(#13222,1:1,000) from Cell Signaling, β-Actin (#AC026, 1:50,000) from ABclonal, SLC1A5 (#ab187692, 1:2,000),JMJD6 (#ab64575, 1:1,000) from Abcam, PRMT1(#11279-1, 1:2,000) and PRMT5 (#18436-1, 1:2,000)from proteintech. .. Protein signals were developed with ultra high sensitive ECL kit (#HY-K1005, MCE) and imaged using a MiniChemi 610 chemiluminescence imaging system with SageCapture software version 2.17.12.170316 (Sagecreation, China). .. Tissue sections were deparaffinized in xylene, dehydrated in an ethanol gradient, and subjected to antigen retrieval in sodium citrate solution (Servicebio, Wuhan, China) in a heater.

    Article Title: Down-regulation of RhoA inhibits YAP by activating Hippo signaling to promote macrophage M1 polarization and enhance its phagocytosis, thereby inhibiting AML progression in preclinical models.
    Article Snippet: Prepare the loading system, load 30 μg protein per well, perform 329 SDS-PAGE electrophoresis to separate the target protein (concentrating gel at 80 V, 330 separating gel at 120 V), transfer to PVDF membrane, incubate with 5% skimmed 331 milk for 2 h, then add the primary antibody dilution RhoA (ab187027, 1 : 5000, 332 Abcam, Cambridge, UK), LATS1 (ab243656, 1 : 1000, Abcam), p-LATS1 (ab305029, 333 1 : 1000, Abcam), YAP (ab52771, 1 : 10000, Abcam), p-YAP (ab76252, 1 : 10000, 334 Abcam), inducible nitric oxide synthase (iNOS, ab3523, 1 : 1000, Abcam), TNF-α 335 (ab183218, 1 : 2000, Abcam), SIRPα (ab260039, 1 : 1000, Abcam), CD47 (ab218810, 336 1 : 1000, Abcam), Bcl-2-associated x protein (Bax, ab182734, 1 : 1000, Abcam), 337 B-cell lymphoma-2 (Bcl-2, ab16904, 1 : 1000, Abcam), Cleaved Caspase-3 338 (ab214430, 1 : 5000, Abcam), arginase-1 (Arg-1, DF6657, 1 : 2000, Affinity, Jiangsu, 339 China), and GAPDH (ab181603, 1 : 10000, Abcam) at 4°C overnight, and then add 340 the corresponding secondary antibody dilution for 2 hours. .. ECL kit (HY-K1005, 341 MedChemExpress) was used for chemiluminescence detection, and 342 chemiluminescence imaging system (Geldoc Go, Bio-Rad, Shanghai, China) was used 343 Jo urn l P repro of 13 to collect protein band images. ..

    Membrane:

    Article Title: Dexmedetomidine Activates AMPK/SIRT1 Pathway to Reduce Inflammatory Levels and Neuronal Pyroptosis to Alleviate Neonatal Hypoxic-Ischemic Brain Damage.
    Article Snippet: Neonatal hypoxic-ischemic brain damage (HIBD) is the leading cause of neonatal death and long-term neurological dysfunction.. Dexmedetomidine (DEX) has been shown to have neuroprotective effects, but its mechanisms in HIBD remain incompletely understood.. This study aimed to explore whether DEX inhibits neuroinflammation and pyroptosis via activating adenosine monophosphate-activated protein kinase (AMPK)/sirtuin 1 (SIRT1) axis, thereby reducing HIBD.

    Article Title: Down-regulation of THBS2 inhibits the malignant progression of Burkitt lymphoma by blocking the PI3K/AKT/c-MYC pathway
    Article Snippet: .. The PVDF membrane was used for membrane transfer, blocked with 5% skim milk for 2 h, and then incubated with primary antibody dilution at 4°C overnight, and then incubated with the corresponding secondary antibody dilution for 2 h. ECL kit (HY-K1005, MedChemExpress) was used for chemiluminescence detection, and gel imaging system (Geldoc Go, Bio-Rad, Shanghai, China) was used to detect protein bands. ..

    Incubation:

    Article Title: Dexmedetomidine Activates AMPK/SIRT1 Pathway to Reduce Inflammatory Levels and Neuronal Pyroptosis to Alleviate Neonatal Hypoxic-Ischemic Brain Damage.
    Article Snippet: Neonatal hypoxic-ischemic brain damage (HIBD) is the leading cause of neonatal death and long-term neurological dysfunction.. Dexmedetomidine (DEX) has been shown to have neuroprotective effects, but its mechanisms in HIBD remain incompletely understood.. This study aimed to explore whether DEX inhibits neuroinflammation and pyroptosis via activating adenosine monophosphate-activated protein kinase (AMPK)/sirtuin 1 (SIRT1) axis, thereby reducing HIBD.

    Article Title: Down-regulation of THBS2 inhibits the malignant progression of Burkitt lymphoma by blocking the PI3K/AKT/c-MYC pathway
    Article Snippet: .. The PVDF membrane was used for membrane transfer, blocked with 5% skim milk for 2 h, and then incubated with primary antibody dilution at 4°C overnight, and then incubated with the corresponding secondary antibody dilution for 2 h. ECL kit (HY-K1005, MedChemExpress) was used for chemiluminescence detection, and gel imaging system (Geldoc Go, Bio-Rad, Shanghai, China) was used to detect protein bands. ..

    Sensitivity:

    Article Title: Hederacolchiside A1 targets ERO1A to suppress PI3K/AKT/mTOR signaling and induce ferroptosis in colorectal cancer.
    Article Snippet: Colorectal cancer (CRC) remains a major global health challenge due to high incidence, poor prognosis, and limited therapeutic options.. Ferroptosis, an iron-dependent form of regulated cell death, has emerged as a promising anti-tumor strategy, yet pharmacological inducers remain underexplored.. Hederacolchiside A1 (HeA1), a natural bioactive compound, was investigated for its potential anti-CRC effects and underlying mechanisms.

    Western Blot:

    Article Title: Hederacolchiside A1 targets ERO1A to suppress PI3K/AKT/mTOR signaling and induce ferroptosis in colorectal cancer.
    Article Snippet: Colorectal cancer (CRC) remains a major global health challenge due to high incidence, poor prognosis, and limited therapeutic options.. Ferroptosis, an iron-dependent form of regulated cell death, has emerged as a promising anti-tumor strategy, yet pharmacological inducers remain underexplored.. Hederacolchiside A1 (HeA1), a natural bioactive compound, was investigated for its potential anti-CRC effects and underlying mechanisms.

    Software:

    Article Title: Glutamine sequestration promotes tumor growth and immune evasion through elevation of arginine methylation in hepatocellular carcinoma
    Article Snippet: Western blot was performed using primary antibodies against: MMA(#8711,1:1,000), ADMA(#13522,1:1,000), SDMA(#13222,1:1,000) from Cell Signaling, β-Actin (#AC026, 1:50,000) from ABclonal, SLC1A5 (#ab187692, 1:2,000),JMJD6 (#ab64575, 1:1,000) from Abcam, PRMT1(#11279-1, 1:2,000) and PRMT5 (#18436-1, 1:2,000)from proteintech. .. Protein signals were developed with ultra high sensitive ECL kit (#HY-K1005, MCE) and imaged using a MiniChemi 610 chemiluminescence imaging system with SageCapture software version 2.17.12.170316 (Sagecreation, China). .. Tissue sections were deparaffinized in xylene, dehydrated in an ethanol gradient, and subjected to antigen retrieval in sodium citrate solution (Servicebio, Wuhan, China) in a heater.

    Modification:

    Article Title: Quercetin attenuates cisplatin-induced muscle atrophy via Hippo/YAP1 signaling pathway.
    Article Snippet: Dapeng Jiang jdp509@163.com 1 Department of Oncology (Ward 2), Shanghai Childrens Medical Center, Shanghai Jiao Tong University, Shanghai, China Abstract Muscle wasting is a common event among cancer patients receiving chemotherapy treatment and has been reported to affect their survival.. However, current therapies for counteracting this side effect are ineffective.. This study investigates quercetin’s efficacy against cisplatin-induced muscle atrophy and its mechanism.

    Radio Immunoprecipitation:

    Article Title: Quercetin attenuates cisplatin-induced muscle atrophy via Hippo/YAP1 signaling pathway.
    Article Snippet: Dapeng Jiang jdp509@163.com 1 Department of Oncology (Ward 2), Shanghai Childrens Medical Center, Shanghai Jiao Tong University, Shanghai, China Abstract Muscle wasting is a common event among cancer patients receiving chemotherapy treatment and has been reported to affect their survival.. However, current therapies for counteracting this side effect are ineffective.. This study investigates quercetin’s efficacy against cisplatin-induced muscle atrophy and its mechanism.

    Bicinchoninic Acid Protein Assay:

    Article Title: Quercetin attenuates cisplatin-induced muscle atrophy via Hippo/YAP1 signaling pathway.
    Article Snippet: Dapeng Jiang jdp509@163.com 1 Department of Oncology (Ward 2), Shanghai Childrens Medical Center, Shanghai Jiao Tong University, Shanghai, China Abstract Muscle wasting is a common event among cancer patients receiving chemotherapy treatment and has been reported to affect their survival.. However, current therapies for counteracting this side effect are ineffective.. This study investigates quercetin’s efficacy against cisplatin-induced muscle atrophy and its mechanism.

    CCK-8 Assay:

    Article Title: Quercetin attenuates cisplatin-induced muscle atrophy via Hippo/YAP1 signaling pathway.
    Article Snippet: Dapeng Jiang jdp509@163.com 1 Department of Oncology (Ward 2), Shanghai Childrens Medical Center, Shanghai Jiao Tong University, Shanghai, China Abstract Muscle wasting is a common event among cancer patients receiving chemotherapy treatment and has been reported to affect their survival.. However, current therapies for counteracting this side effect are ineffective.. This study investigates quercetin’s efficacy against cisplatin-induced muscle atrophy and its mechanism.

    Protease Inhibitor:

    Article Title: Quercetin attenuates cisplatin-induced muscle atrophy via Hippo/YAP1 signaling pathway.
    Article Snippet: Dapeng Jiang jdp509@163.com 1 Department of Oncology (Ward 2), Shanghai Childrens Medical Center, Shanghai Jiao Tong University, Shanghai, China Abstract Muscle wasting is a common event among cancer patients receiving chemotherapy treatment and has been reported to affect their survival.. However, current therapies for counteracting this side effect are ineffective.. This study investigates quercetin’s efficacy against cisplatin-induced muscle atrophy and its mechanism.

    Expressing:

    Article Title: Targeted modulation of Kupffer cell-monocyte communication by engineered extracellular vesicles to alleviate immunological liver injury
    Article Snippet: After blocking with 5% milk for 1 h, the membranes were incubated with primary antibodies (anti-TSG101 (ET1701-59, HuaBio), anti-ALIX (ET1705-74, HuaBio), anti-HSP70 (ET1601-11, HuaBio), anti-CytC (ET1610-60, HuaBio), anti-CD63 (25682-1-AP, Proteintech), anti-P-IKBα (ET1609-78, HuaBio), anti-IKBα (1603-6, HuaBio), anti-P-NF-κB P65 (HA723223, HuaBio), anti-NF-κB P65 (ET1603, HuaBio) at 4 °C overnight, followed by secondary antibody treatment (HRP-conjugated, Proteintech) for 1 h at a room temperature. .. Band detection was conducted with an ultrahigh-sensitivity ECL kit (MedChemExpress, USA), and ImageJ software was used to quantify expression levels. .. RNA was extracted from cells and liver tissues by a RNA extraction kit (Axygen, USA), followed by cDNA synthesis via the PrimeScript RT reagent kit (Takara, Japan).



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