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Affinity Biosciences ecl development system
Ecl Development System, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecl+developer/ecl+hypersensitive+kit/pmc12756082-79-0-3
Average 86 stars, based on 1 article reviews
ecl development system - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Control:

Article Title: CMTM4 overexpression inhibited tumor growth through regulating the function of myeloid-derived suppressor cells in the cervical cancer microenvironment
Article Snippet: Following three washes with TBST (Tris-buffered saline containing 0.1% Tween), the membranes were incubated for 1.5 h with HRP-conjugated goat anti-rabbit IgG (Abcam, Cambridge, UK; cat. no. ab6721). .. Protein bands were detected using an ECL system (Affinity Biosciences, Cincinnati, OH, USA) with β-actin serving as the loading control. .. Band intensities were quantified using Quantity One software (Bio-Rad).

Incubation:

Article Title: Pinocembrin Ameliorates Skin Fibrosis via Inhibiting TGF-β1 Signaling Pathway.
Article Snippet: .. Then, the membranes were incubated with HRPconjugated secondary antibodies and detected with an ECL system (Affinity Bioscience, Cincinnati, OH, United States). ..

Article Title: Berberine from Tibetan Medicine Dracocephalum tanguticum Maxim Suppresses Colorectal Tumor Growth and Inhibits CD8⁺ T Cells Ferroptosis via the NRF2–SLC7A11–GPX4 Axis
Article Snippet: .. After washing, membranes were incubated with HRP-conjugated secondary antibodies, and protein bands were detected using an ECL kit (Affinity Biosciences). ..

Article Title: Decoding the exosomal nucleic acid delivery system axis of macrophage autophagy and immune reprogramming via multi-omics analysis
Article Snippet: .. ECL development system (Affinity Biosciences, Cincinnati, OH, USA, catalog #KF8003): The PVDF membrane was soaked in TBST containing 5% non-fat milk (blocking buffer) and blocked on a shaker at room temperature for 2 h. The corresponding primary antibody was diluted with blocking buffer, and the PVDF membrane was immersed in the primary antibody incubation solution and incubated overnight at 4 °C. ..

Membrane:

Article Title: Curcumin/piperine modulates the butyrate levels and alleviates diabetic kidney disease via Nrf2/HO-1 signaling pathway
Article Snippet: .. The following day, after washing the membrane with TBST for 30 min, incubate with goat anti-rabbit IgG (H + L) secondary antibody HRP conjugate for 90 min. After another TBST wash, protein visualization was performed using a hypersensitive ECL kit (Affinity Biosciences, Liyang City, China). .. GraphPad prism 9.5 (La Jolla, CA) was used for statistical analysis of the data.

Article Title: Decoding the exosomal nucleic acid delivery system axis of macrophage autophagy and immune reprogramming via multi-omics analysis
Article Snippet: .. ECL development system (Affinity Biosciences, Cincinnati, OH, USA, catalog #KF8003): The PVDF membrane was soaked in TBST containing 5% non-fat milk (blocking buffer) and blocked on a shaker at room temperature for 2 h. The corresponding primary antibody was diluted with blocking buffer, and the PVDF membrane was immersed in the primary antibody incubation solution and incubated overnight at 4 °C. ..

Blocking Assay:

Article Title: Decoding the exosomal nucleic acid delivery system axis of macrophage autophagy and immune reprogramming via multi-omics analysis
Article Snippet: .. ECL development system (Affinity Biosciences, Cincinnati, OH, USA, catalog #KF8003): The PVDF membrane was soaked in TBST containing 5% non-fat milk (blocking buffer) and blocked on a shaker at room temperature for 2 h. The corresponding primary antibody was diluted with blocking buffer, and the PVDF membrane was immersed in the primary antibody incubation solution and incubated overnight at 4 °C. ..



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