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ecl developing solution  (Bio-Rad)


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    Structured Review

    Bio-Rad ecl developing solution
    Ecl Developing Solution, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 15580 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecl+developer/Clarity+Western+ECL+Substrate/pm41242627-183-24-27
    Average 99 stars, based on 15580 article reviews
    ecl developing solution - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Western Blot:

    Article Title: Development of a high-throughput screening platform for identification of functional BACH1 inhibitors reveals compounds with anti-invasive potential
    Article Snippet: .. Following another set of TBST washes, the bound secondary antibodies were detected by enhanced chemiluminescence using ClarityTM Western ECL Blotting Substrate (Bio-Rad, Hercules, CA, USA). ..

    Article Title: Ketone body metabolism activates the immune response against Staphylococcus aureus infection by fueling the tricarboxylic acid cycle and affecting histone β-hydroxybutyrylation
    Article Snippet: .. Protein bands were visualized using the Clarity Western ECL Substrate (Bio-Rad, Hercules, CA, USA) on an LAS-4000 imaging system (Fujifilm, Tokyo, Japan). ..

    Article Title: Enhanced AAV production via rational design of a novel pHelper vector integrated with HSV-1 helper genes
    Article Snippet: HRP-conjugated secondary antibodies (anti-mouse IgG, Cell Signaling Technology, USA) were used at a 1 in 5000 dilution. .. The chemiluminescent signal was detected with Clarity Western ECL reagent (Bio-Rad Laboratories, UK) using iBright CL1500 system (ThermoFisher Scientific, USA). ..

    Article Title: Nitric oxide-dependent stabilization of vimentin confers chemoresistance in ovarian cancer
    Article Snippet: The membranes were blocked with 5% non-fat milk and incubated with primary antibody at 4°C (rabbit anti-iNOS [1:1,000, Cell Signaling, Cat. #95423], rabbit anti-vimentin [1:1,000, Cell Signaling, Cat. #5741], rabbit anti-B-actin horseradish peroxidase (HRP)-conjugated [1:7,000, Cell Signaling, Cat. #5125]) and then incubated with a secondary anti-rabbit horseradish peroxidase-conjugated antibody (secondary anti-rabbit HRP-conjugated antibody [1:3,000, Cell Signaling, Cat #7074]). .. Chemiluminescence signals were detected using Clarity ECL Western Blotting Substrates from Bio-Rad (Cat #1705060S). ..

    Article Title: Secreted enzyme uptake masks the in vivo phenotype of macrophage-specific lysosomal acid lipase deletion
    Article Snippet: .. Secondary polyclonal goat anti-rabbit antibody (P0448, 1:1,000; Agilent Technologies, Inc., Santa Clara, CA) conjugated to HRP was visualized using the Clarity Western ECL Substrate (Bio-Rad Laboratories, Hercules, CA) on a ChemiDocTM imaging system (Bio-Rad Laboratories). ..

    Article Title: Development of a PTEN -siRNA activated scaffold to promote axonal regrowth following spinal cord injury
    Article Snippet: .. The next day, each membrane was washed three times in TBST, incubated with a goat anti-rabbit HRP secondary antibody for 1 h at room temperature (1:10000; Abcam) and the protein bands visualised using ClarityTM Western ECL Substrate (BioRad). ..

    Article Title: Genetic inactivation of TRPM4 does not alter the temperature-dependent movement of mouse microglia
    Article Snippet: After three washes with phosphate buffered saline with Tween 20 (PBS-T), the membrane was incubated for 1 h at room temperature with an anti-rabbit IgG horseradish peroxidase (HRP)-linked antibody (1:1000, Cell Signaling Technology). .. After incubating the membrane for 5 min with Clarity Western ECL substrate (BioRad), proteins were detected with a molecular imager (ChemiDoc XRS+ imaging system, BioRad). .. Glass bottom dishes containing primary mouse microglia were placed on the stage of an inverted Keyence BZ-X700 (Keyence) fluorescence microscope fitted with a temperature-controlled stage incubator (Tokai Hit) under a circulating mixture of gases (20% O 2 , 5% CO 2 , and 75% N 2 ).

    Imaging:

    Article Title: Ketone body metabolism activates the immune response against Staphylococcus aureus infection by fueling the tricarboxylic acid cycle and affecting histone β-hydroxybutyrylation
    Article Snippet: .. Protein bands were visualized using the Clarity Western ECL Substrate (Bio-Rad, Hercules, CA, USA) on an LAS-4000 imaging system (Fujifilm, Tokyo, Japan). ..

    Article Title: Secreted enzyme uptake masks the in vivo phenotype of macrophage-specific lysosomal acid lipase deletion
    Article Snippet: .. Secondary polyclonal goat anti-rabbit antibody (P0448, 1:1,000; Agilent Technologies, Inc., Santa Clara, CA) conjugated to HRP was visualized using the Clarity Western ECL Substrate (Bio-Rad Laboratories, Hercules, CA) on a ChemiDocTM imaging system (Bio-Rad Laboratories). ..

    Article Title: Genetic inactivation of TRPM4 does not alter the temperature-dependent movement of mouse microglia
    Article Snippet: After three washes with phosphate buffered saline with Tween 20 (PBS-T), the membrane was incubated for 1 h at room temperature with an anti-rabbit IgG horseradish peroxidase (HRP)-linked antibody (1:1000, Cell Signaling Technology). .. After incubating the membrane for 5 min with Clarity Western ECL substrate (BioRad), proteins were detected with a molecular imager (ChemiDoc XRS+ imaging system, BioRad). .. Glass bottom dishes containing primary mouse microglia were placed on the stage of an inverted Keyence BZ-X700 (Keyence) fluorescence microscope fitted with a temperature-controlled stage incubator (Tokai Hit) under a circulating mixture of gases (20% O 2 , 5% CO 2 , and 75% N 2 ).

    Transfection:

    Article Title: Targeted mutagenesis of the ICP4 transactivation domain generates a protective DIVA vaccine against duck plague
    Article Snippet: Alexa Fluor 488-conjugated goat anti-rabbit IgG (H + L) secondary antibody (Invitrogen, A11008) and Alexa Fluor 568-conjugated goat anti-mouse IgG (H + L) secondary antibody (Invitrogen, A11004). .. The other reagents used and their sources were as follows: liposome transfection reagent (YESEN, 40802ES03), TransDetect Double-Luciferase Reporter Assay Kit (Transgen, TR202-02), RNA-easy TM Isolation Reagent (Vazyme, R701-01), SuperMix for qPCR (gDNA digester plus) (YEASEN, 11141ES60), qPCR SYBR Green Master Mix (YEASEN, 11184ES08), protein A/G Magnetic Beads (MedChemExpress, HY-K02),Sonication ChIP Kit (Abclonal, RK20258), DNAiso reagent (Takara, Cat No. 9770A), HiPure Viral DNA Kit (Magen, Cat No. 3191), ClonExpress II One Step Cloning Kit (Vazyme, Cat No. C112-01), Clarity Western ECL substrate (Bio-Rad, Cat No. 1705061). ..

    Reporter Assay:

    Article Title: Targeted mutagenesis of the ICP4 transactivation domain generates a protective DIVA vaccine against duck plague
    Article Snippet: Alexa Fluor 488-conjugated goat anti-rabbit IgG (H + L) secondary antibody (Invitrogen, A11008) and Alexa Fluor 568-conjugated goat anti-mouse IgG (H + L) secondary antibody (Invitrogen, A11004). .. The other reagents used and their sources were as follows: liposome transfection reagent (YESEN, 40802ES03), TransDetect Double-Luciferase Reporter Assay Kit (Transgen, TR202-02), RNA-easy TM Isolation Reagent (Vazyme, R701-01), SuperMix for qPCR (gDNA digester plus) (YEASEN, 11141ES60), qPCR SYBR Green Master Mix (YEASEN, 11184ES08), protein A/G Magnetic Beads (MedChemExpress, HY-K02),Sonication ChIP Kit (Abclonal, RK20258), DNAiso reagent (Takara, Cat No. 9770A), HiPure Viral DNA Kit (Magen, Cat No. 3191), ClonExpress II One Step Cloning Kit (Vazyme, Cat No. C112-01), Clarity Western ECL substrate (Bio-Rad, Cat No. 1705061). ..

    Isolation:

    Article Title: Targeted mutagenesis of the ICP4 transactivation domain generates a protective DIVA vaccine against duck plague
    Article Snippet: Alexa Fluor 488-conjugated goat anti-rabbit IgG (H + L) secondary antibody (Invitrogen, A11008) and Alexa Fluor 568-conjugated goat anti-mouse IgG (H + L) secondary antibody (Invitrogen, A11004). .. The other reagents used and their sources were as follows: liposome transfection reagent (YESEN, 40802ES03), TransDetect Double-Luciferase Reporter Assay Kit (Transgen, TR202-02), RNA-easy TM Isolation Reagent (Vazyme, R701-01), SuperMix for qPCR (gDNA digester plus) (YEASEN, 11141ES60), qPCR SYBR Green Master Mix (YEASEN, 11184ES08), protein A/G Magnetic Beads (MedChemExpress, HY-K02),Sonication ChIP Kit (Abclonal, RK20258), DNAiso reagent (Takara, Cat No. 9770A), HiPure Viral DNA Kit (Magen, Cat No. 3191), ClonExpress II One Step Cloning Kit (Vazyme, Cat No. C112-01), Clarity Western ECL substrate (Bio-Rad, Cat No. 1705061). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Targeted mutagenesis of the ICP4 transactivation domain generates a protective DIVA vaccine against duck plague
    Article Snippet: Alexa Fluor 488-conjugated goat anti-rabbit IgG (H + L) secondary antibody (Invitrogen, A11008) and Alexa Fluor 568-conjugated goat anti-mouse IgG (H + L) secondary antibody (Invitrogen, A11004). .. The other reagents used and their sources were as follows: liposome transfection reagent (YESEN, 40802ES03), TransDetect Double-Luciferase Reporter Assay Kit (Transgen, TR202-02), RNA-easy TM Isolation Reagent (Vazyme, R701-01), SuperMix for qPCR (gDNA digester plus) (YEASEN, 11141ES60), qPCR SYBR Green Master Mix (YEASEN, 11184ES08), protein A/G Magnetic Beads (MedChemExpress, HY-K02),Sonication ChIP Kit (Abclonal, RK20258), DNAiso reagent (Takara, Cat No. 9770A), HiPure Viral DNA Kit (Magen, Cat No. 3191), ClonExpress II One Step Cloning Kit (Vazyme, Cat No. C112-01), Clarity Western ECL substrate (Bio-Rad, Cat No. 1705061). ..

    SYBR Green Assay:

    Article Title: Targeted mutagenesis of the ICP4 transactivation domain generates a protective DIVA vaccine against duck plague
    Article Snippet: Alexa Fluor 488-conjugated goat anti-rabbit IgG (H + L) secondary antibody (Invitrogen, A11008) and Alexa Fluor 568-conjugated goat anti-mouse IgG (H + L) secondary antibody (Invitrogen, A11004). .. The other reagents used and their sources were as follows: liposome transfection reagent (YESEN, 40802ES03), TransDetect Double-Luciferase Reporter Assay Kit (Transgen, TR202-02), RNA-easy TM Isolation Reagent (Vazyme, R701-01), SuperMix for qPCR (gDNA digester plus) (YEASEN, 11141ES60), qPCR SYBR Green Master Mix (YEASEN, 11184ES08), protein A/G Magnetic Beads (MedChemExpress, HY-K02),Sonication ChIP Kit (Abclonal, RK20258), DNAiso reagent (Takara, Cat No. 9770A), HiPure Viral DNA Kit (Magen, Cat No. 3191), ClonExpress II One Step Cloning Kit (Vazyme, Cat No. C112-01), Clarity Western ECL substrate (Bio-Rad, Cat No. 1705061). ..

    Magnetic Beads:

    Article Title: Targeted mutagenesis of the ICP4 transactivation domain generates a protective DIVA vaccine against duck plague
    Article Snippet: Alexa Fluor 488-conjugated goat anti-rabbit IgG (H + L) secondary antibody (Invitrogen, A11008) and Alexa Fluor 568-conjugated goat anti-mouse IgG (H + L) secondary antibody (Invitrogen, A11004). .. The other reagents used and their sources were as follows: liposome transfection reagent (YESEN, 40802ES03), TransDetect Double-Luciferase Reporter Assay Kit (Transgen, TR202-02), RNA-easy TM Isolation Reagent (Vazyme, R701-01), SuperMix for qPCR (gDNA digester plus) (YEASEN, 11141ES60), qPCR SYBR Green Master Mix (YEASEN, 11184ES08), protein A/G Magnetic Beads (MedChemExpress, HY-K02),Sonication ChIP Kit (Abclonal, RK20258), DNAiso reagent (Takara, Cat No. 9770A), HiPure Viral DNA Kit (Magen, Cat No. 3191), ClonExpress II One Step Cloning Kit (Vazyme, Cat No. C112-01), Clarity Western ECL substrate (Bio-Rad, Cat No. 1705061). ..

    Chromatin Immunoprecipitation:

    Article Title: Targeted mutagenesis of the ICP4 transactivation domain generates a protective DIVA vaccine against duck plague
    Article Snippet: Alexa Fluor 488-conjugated goat anti-rabbit IgG (H + L) secondary antibody (Invitrogen, A11008) and Alexa Fluor 568-conjugated goat anti-mouse IgG (H + L) secondary antibody (Invitrogen, A11004). .. The other reagents used and their sources were as follows: liposome transfection reagent (YESEN, 40802ES03), TransDetect Double-Luciferase Reporter Assay Kit (Transgen, TR202-02), RNA-easy TM Isolation Reagent (Vazyme, R701-01), SuperMix for qPCR (gDNA digester plus) (YEASEN, 11141ES60), qPCR SYBR Green Master Mix (YEASEN, 11184ES08), protein A/G Magnetic Beads (MedChemExpress, HY-K02),Sonication ChIP Kit (Abclonal, RK20258), DNAiso reagent (Takara, Cat No. 9770A), HiPure Viral DNA Kit (Magen, Cat No. 3191), ClonExpress II One Step Cloning Kit (Vazyme, Cat No. C112-01), Clarity Western ECL substrate (Bio-Rad, Cat No. 1705061). ..

    Cloning:

    Article Title: Targeted mutagenesis of the ICP4 transactivation domain generates a protective DIVA vaccine against duck plague
    Article Snippet: Alexa Fluor 488-conjugated goat anti-rabbit IgG (H + L) secondary antibody (Invitrogen, A11008) and Alexa Fluor 568-conjugated goat anti-mouse IgG (H + L) secondary antibody (Invitrogen, A11004). .. The other reagents used and their sources were as follows: liposome transfection reagent (YESEN, 40802ES03), TransDetect Double-Luciferase Reporter Assay Kit (Transgen, TR202-02), RNA-easy TM Isolation Reagent (Vazyme, R701-01), SuperMix for qPCR (gDNA digester plus) (YEASEN, 11141ES60), qPCR SYBR Green Master Mix (YEASEN, 11184ES08), protein A/G Magnetic Beads (MedChemExpress, HY-K02),Sonication ChIP Kit (Abclonal, RK20258), DNAiso reagent (Takara, Cat No. 9770A), HiPure Viral DNA Kit (Magen, Cat No. 3191), ClonExpress II One Step Cloning Kit (Vazyme, Cat No. C112-01), Clarity Western ECL substrate (Bio-Rad, Cat No. 1705061). ..

    Membrane:

    Article Title: Development of a PTEN -siRNA activated scaffold to promote axonal regrowth following spinal cord injury
    Article Snippet: .. The next day, each membrane was washed three times in TBST, incubated with a goat anti-rabbit HRP secondary antibody for 1 h at room temperature (1:10000; Abcam) and the protein bands visualised using ClarityTM Western ECL Substrate (BioRad). ..

    Article Title: Genetic inactivation of TRPM4 does not alter the temperature-dependent movement of mouse microglia
    Article Snippet: After three washes with phosphate buffered saline with Tween 20 (PBS-T), the membrane was incubated for 1 h at room temperature with an anti-rabbit IgG horseradish peroxidase (HRP)-linked antibody (1:1000, Cell Signaling Technology). .. After incubating the membrane for 5 min with Clarity Western ECL substrate (BioRad), proteins were detected with a molecular imager (ChemiDoc XRS+ imaging system, BioRad). .. Glass bottom dishes containing primary mouse microglia were placed on the stage of an inverted Keyence BZ-X700 (Keyence) fluorescence microscope fitted with a temperature-controlled stage incubator (Tokai Hit) under a circulating mixture of gases (20% O 2 , 5% CO 2 , and 75% N 2 ).

    Incubation:

    Article Title: Development of a PTEN -siRNA activated scaffold to promote axonal regrowth following spinal cord injury
    Article Snippet: .. The next day, each membrane was washed three times in TBST, incubated with a goat anti-rabbit HRP secondary antibody for 1 h at room temperature (1:10000; Abcam) and the protein bands visualised using ClarityTM Western ECL Substrate (BioRad). ..



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