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GENEFLOW LIMITED ez-ecl chemiluminescence detection kit
Ez Ecl Chemiluminescence Detection Kit, supplied by GENEFLOW LIMITED, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecl+detection+kit/ez+ecl+chemiluminescence+detection+kit/pm34007040-415-5-9
Average 90 stars, based on 1 article reviews
ez-ecl chemiluminescence detection kit - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Phosphoinositide 3-Kinase Alpha-Dependent Regulation of Branching Morphogenesis in Murine Embryonic Lung: Evidence for a Role in Determining Morphogenic Properties of FGF7
Article Snippet: Membranes were then incubated in an HRP-conjugated goat anti-rabbit secondary antibody (DAKO) before bands were visualised using an EZ-ECL chemiluminescence detection kit (Geneflow, Staffordshire, UK) with an ImageQuant developer (GE healthcare, Buckinghamshire, UK).

Article Title: Primary skeletal muscle cells from chronic kidney disease patients retain hallmarks of cachexia in vitro
Article Snippet: Following washing, membranes were incubated with species specific horseradish peroxides conjugated secondary antibodies and visualized using EZ‐chemiluminescence detection kit (Geneflow, Lichfield, UK).

Article Title: Hepatitis A Virus Cellular Receptor 1 (HAVcr-1) Initiates Prostate Cancer Progression in Human Cells via Hepatocyte Growth Factor (HGF)-Induced Changes in Junctional Integrity.
Article Snippet: EZ-ECL Chemiluminescent Detection Kit (Geneflow, Staffordshire, UK) was used for protein visualization.

Article Title: Optimisation of the foot-and-mouth disease virus 2A co-expression system for biomedical applications
Article Snippet: The following reagents were obtained from commercial suppliers: Pfu polymerase, T4 DNA ligase, restriction enzymes, JM109 competent cells, Quick TnT rabbit reticulocyte lysate kit (Promega, Madison, WI, USA); S 35 methionine (MP Biomedicals, Santa Ana, CA, USA); EDTA-free protease inhibitor cocktail (Sigma-Aldrich Company Ltd, Dorset, UK); pcDNA3.1, anti-RFP, rabbit purified polyclonal antibody against Red Fluorescent Protein (1:1000 dilution), anti-GFP, mAb 3E6, anti-Green Fluorescent Protein, mouse monoclonal antibody (1:1000 dilution), anti-β-tubulin, mouse monoclonal antibody (1:2000 dilution), Lipofectamine2000 transfection reagent, iBlot Gel Transfer Stacks, nitrocellulose (Life Technologies Ltd, Paisley, UK); secondary HRP-conjugated polyclonal goat anti-mouse and anti-rabbit antibodies (Dako UK Ltd, Ely, UK); rabbit anti-FMDV 2A antiserum raised against the synthetic peptide NH2-LLNFDLLKLAGDVESNPGP-COOH; EZ-ECL Chemiluminescence Detection Kit for HRP (Geneflow Ltd, Elmhurst, UK); VECTASHIELD mounting medium with DAPI (VECTOR Laboratories Ltd, Peterborough, UK); Oligonucleotides were purchased from Integrated DNA Technologies (Coralville, Iowa, USA).

Article Title: Multiple ubiquitin E3 ligase genes antagonistically regulate chloroplast-associated protein degradation
Article Snippet: EZ-Chemiluminescence Detection Kit for HRP , Geneflow , Cat#K1-0170.

Article Title: Multiple ubiquitin E3 ligase genes antagonistically regulate chloroplast-associated protein degradation
Article Snippet: Bands were detected using an EZ-Chemiluminescence Detection Kit for HRP (Geneflow), and recorded using an LAS-4000 imager (GE Healthcare).

Incubation:

Article Title: ATG5 is dispensable for ATG8ylation of cellular proteins
Article Snippet: Samples were transferred to Immobilon-FL PVDF membrane (Merck-Millipore) prior to blocking in 5% milk in PBS-T (0.1% Tween-20), overnight primary antibody incubation at 4°C, washing in PBS-T and secondary antibody incubation for 1 hour at room temperature. .. After washing, blots incubated with HPR-conjugated secondary antibodies were developed using HRP substrate (EZ-ECL chemiluminescence detection kit for HRP, Geneflow Ltd.) followed by exposure on an ImageQuant chemiluminescent imaging system (GE Healthcare). .. For blots probed with fluorescent secondary antibodies, detection was performed using an Odyssey IR imaging system (LI-COR Biosciences).

Imaging:

Article Title: ATG5 is dispensable for ATG8ylation of cellular proteins
Article Snippet: Samples were transferred to Immobilon-FL PVDF membrane (Merck-Millipore) prior to blocking in 5% milk in PBS-T (0.1% Tween-20), overnight primary antibody incubation at 4°C, washing in PBS-T and secondary antibody incubation for 1 hour at room temperature. .. After washing, blots incubated with HPR-conjugated secondary antibodies were developed using HRP substrate (EZ-ECL chemiluminescence detection kit for HRP, Geneflow Ltd.) followed by exposure on an ImageQuant chemiluminescent imaging system (GE Healthcare). .. For blots probed with fluorescent secondary antibodies, detection was performed using an Odyssey IR imaging system (LI-COR Biosciences).



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