Review



ecl  (Cyanagen)


Bioz Verified Symbol Cyanagen is a verified supplier
Bioz Manufacturer Symbol Cyanagen manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Cyanagen ecl
    Ecl, supplied by Cyanagen, used in various techniques. Bioz Stars score: 99/100, based on 566 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecl+a/ecl/custom%40ecl%4042397737
    Average 99 stars, based on 566 article reviews
    ecl - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: 5-Iodotubercidin inhibits Epithelial to Mesenchymal Transition by inhibiting IKK/NFκB-dependent gene expression.
    Article Snippet: llowed by incubation with the primary antibodies at 4 °C overnight. Blots were 1 washed, incubated with secondary antibodies for one hour at RT and were developed using ECL 2 reagents (Westarnova 2.0, Cyanagen; Clarity Max, Bio-Rad) and bands were visualized using Fusion 3 Solo 6S chemiluminescence imager (Vilber). Densitometric analysis was performed using ImageJ v. 4 1.54d (https://imagej.nih.gov

    Incubation:

    Article Title: 5-Iodotubercidin inhibits Epithelial to Mesenchymal Transition by inhibiting IKK/NFκB-dependent gene expression.
    Article Snippet: llowed by incubation with the primary antibodies at 4 °C overnight. Blots were 1 washed, incubated with secondary antibodies for one hour at RT and were developed using ECL 2 reagents (Westarnova 2.0, Cyanagen; Clarity Max, Bio-Rad) and bands were visualized using Fusion 3 Solo 6S chemiluminescence imager (Vilber). Densitometric analysis was performed using ImageJ v. 4 1.54d (https://imagej.nih.gov

    Cell Culture:

    Article Title: 5-Iodotubercidin inhibits Epithelial to Mesenchymal Transition by inhibiting IKK/NFκB-dependent gene expression.
    Article Snippet: llowed by incubation with the primary antibodies at 4 °C overnight. Blots were 1 washed, incubated with secondary antibodies for one hour at RT and were developed using ECL 2 reagents (Westarnova 2.0, Cyanagen; Clarity Max, Bio-Rad) and bands were visualized using Fusion 3 Solo 6S chemiluminescence imager (Vilber). Densitometric analysis was performed using ImageJ v. 4 1.54d (https://imagej.nih.gov

    Software:

    Article Title: 5-Iodotubercidin inhibits Epithelial to Mesenchymal Transition by inhibiting IKK/NFκB-dependent gene expression.
    Article Snippet: llowed by incubation with the primary antibodies at 4 °C overnight. Blots were 1 washed, incubated with secondary antibodies for one hour at RT and were developed using ECL 2 reagents (Westarnova 2.0, Cyanagen; Clarity Max, Bio-Rad) and bands were visualized using Fusion 3 Solo 6S chemiluminescence imager (Vilber). Densitometric analysis was performed using ImageJ v. 4 1.54d (https://imagej.nih.gov

    Control:

    Article Title: 5-Iodotubercidin inhibits Epithelial to Mesenchymal Transition by inhibiting IKK/NFκB-dependent gene expression.
    Article Snippet: llowed by incubation with the primary antibodies at 4 °C overnight. Blots were 1 washed, incubated with secondary antibodies for one hour at RT and were developed using ECL 2 reagents (Westarnova 2.0, Cyanagen; Clarity Max, Bio-Rad) and bands were visualized using Fusion 3 Solo 6S chemiluminescence imager (Vilber). Densitometric analysis was performed using ImageJ v. 4 1.54d (https://imagej.nih.gov

    Milk:

    Article Title: 5-Iodotubercidin inhibits Epithelial to Mesenchymal Transition by inhibiting IKK/NFκB-dependent gene expression.
    Article Snippet: llowed by incubation with the primary antibodies at 4 °C overnight. Blots were 1 washed, incubated with secondary antibodies for one hour at RT and were developed using ECL 2 reagents (Westarnova 2.0, Cyanagen; Clarity Max, Bio-Rad) and bands were visualized using Fusion 3 Solo 6S chemiluminescence imager (Vilber). Densitometric analysis was performed using ImageJ v. 4 1.54d (https://imagej.nih.gov

    Western Blot:

    Article Title: 5-Iodotubercidin inhibits Epithelial to Mesenchymal Transition by inhibiting IKK/NFκB-dependent gene expression.
    Article Snippet: llowed by incubation with the primary antibodies at 4 °C overnight. Blots were 1 washed, incubated with secondary antibodies for one hour at RT and were developed using ECL 2 reagents (Westarnova 2.0, Cyanagen; Clarity Max, Bio-Rad) and bands were visualized using Fusion 3 Solo 6S chemiluminescence imager (Vilber). Densitometric analysis was performed using ImageJ v. 4 1.54d (https://imagej.nih.gov

    Modification:

    Article Title: 5-Iodotubercidin inhibits Epithelial to Mesenchymal Transition by inhibiting IKK/NFκB-dependent gene expression.
    Article Snippet: llowed by incubation with the primary antibodies at 4 °C overnight. Blots were 1 washed, incubated with secondary antibodies for one hour at RT and were developed using ECL 2 reagents (Westarnova 2.0, Cyanagen; Clarity Max, Bio-Rad) and bands were visualized using Fusion 3 Solo 6S chemiluminescence imager (Vilber). Densitometric analysis was performed using ImageJ v. 4 1.54d (https://imagej.nih.gov

    Extraction:

    Article Title: 5-Iodotubercidin inhibits Epithelial to Mesenchymal Transition by inhibiting IKK/NFκB-dependent gene expression.
    Article Snippet: llowed by incubation with the primary antibodies at 4 °C overnight. Blots were 1 washed, incubated with secondary antibodies for one hour at RT and were developed using ECL 2 reagents (Westarnova 2.0, Cyanagen; Clarity Max, Bio-Rad) and bands were visualized using Fusion 3 Solo 6S chemiluminescence imager (Vilber). Densitometric analysis was performed using ImageJ v. 4 1.54d (https://imagej.nih.gov

    Protein Extraction:

    Article Title: 5-Iodotubercidin inhibits Epithelial to Mesenchymal Transition by inhibiting IKK/NFκB-dependent gene expression.
    Article Snippet: llowed by incubation with the primary antibodies at 4 °C overnight. Blots were 1 washed, incubated with secondary antibodies for one hour at RT and were developed using ECL 2 reagents (Westarnova 2.0, Cyanagen; Clarity Max, Bio-Rad) and bands were visualized using Fusion 3 Solo 6S chemiluminescence imager (Vilber). Densitometric analysis was performed using ImageJ v. 4 1.54d (https://imagej.nih.gov

    Real-time Polymerase Chain Reaction:

    Article Title: 5-Iodotubercidin inhibits Epithelial to Mesenchymal Transition by inhibiting IKK/NFκB-dependent gene expression.
    Article Snippet: llowed by incubation with the primary antibodies at 4 °C overnight. Blots were 1 washed, incubated with secondary antibodies for one hour at RT and were developed using ECL 2 reagents (Westarnova 2.0, Cyanagen; Clarity Max, Bio-Rad) and bands were visualized using Fusion 3 Solo 6S chemiluminescence imager (Vilber). Densitometric analysis was performed using ImageJ v. 4 1.54d (https://imagej.nih.gov

    Membrane:

    Article Title: 5-Iodotubercidin inhibits Epithelial to Mesenchymal Transition by inhibiting IKK/NFκB-dependent gene expression.
    Article Snippet: llowed by incubation with the primary antibodies at 4 °C overnight. Blots were 1 washed, incubated with secondary antibodies for one hour at RT and were developed using ECL 2 reagents (Westarnova 2.0, Cyanagen; Clarity Max, Bio-Rad) and bands were visualized using Fusion 3 Solo 6S chemiluminescence imager (Vilber). Densitometric analysis was performed using ImageJ v. 4 1.54d (https://imagej.nih.gov

    Gentle:

    Article Title: 5-Iodotubercidin inhibits Epithelial to Mesenchymal Transition by inhibiting IKK/NFκB-dependent gene expression.
    Article Snippet: llowed by incubation with the primary antibodies at 4 °C overnight. Blots were 1 washed, incubated with secondary antibodies for one hour at RT and were developed using ECL 2 reagents (Westarnova 2.0, Cyanagen; Clarity Max, Bio-Rad) and bands were visualized using Fusion 3 Solo 6S chemiluminescence imager (Vilber). Densitometric analysis was performed using ImageJ v. 4 1.54d (https://imagej.nih.gov



    Similar Products

    98
    Cytiva Europe amersham ecl prime western blot detection reagent
    Amersham Ecl Prime Western Blot Detection Reagent, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecl+a/Amersham+ECL+Prime+Western+blot+detection+reagent/custom%40rpn2232%4010%2E22175%2Fmmb%2E15701
    Average 98 stars, based on 1 article reviews
    amersham ecl prime western blot detection reagent - by Bioz Stars, 2026-10
    98/100 stars
      Buy from Supplier

    96
    Cytiva Europe amersham ecl western blot detection reagent
    Amersham Ecl Western Blot Detection Reagent, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecl+a/Amersham+ECL+Western+blot+detection+reagent/custom%40rpn2106%4041046976
    Average 96 stars, based on 1 article reviews
    amersham ecl western blot detection reagent - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    99
    Bio-Rad ecl solution
    Ecl Solution, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecl+a/Clarity+ECL+Western+Substrate/pm42898112-257-241-243
    Average 99 stars, based on 1 article reviews
    ecl solution - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    97
    Bio-Rad chemiluminescence
    Chemiluminescence, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecl+a/Clarity+Max+Western+ECL+Substrate/pm42894850-242-4-5
    Average 97 stars, based on 1 article reviews
    chemiluminescence - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    97
    Bio-Rad clarity max western ecl substrate system
    Clarity Max Western Ecl Substrate System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecl+a/Clarity+Max+Western+ECL+Substrate/pmc13676815-438-27-35
    Average 97 stars, based on 1 article reviews
    clarity max western ecl substrate system - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    99
    Bio-Rad clarity western ecl substrate
    Clarity Western Ecl Substrate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecl+a/Clarity+Western+ECL+Substrate/pmc13676678-87-5-9
    Average 99 stars, based on 1 article reviews
    clarity western ecl substrate - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad clarity western ecl substrate bio rad
    Targeted activation of endogenous mitochondrial ClpP. (A) Western blots of mitochondrial lysates (30 μg) from wild‐type and ClpP‐KO HEK293T cells were probed with antibodies against ClpP and α‐tubulin, the latter being used as a loading control. (B) Analysis of ClpP and ClpX assembly was carried out in wild‐type HEK293T mitochondria through isokinetic sucrose gradient centrifugation. Mitochondrial lysate (0.65 mg) was centrifuged on a 10%–30% gradient using the SW 60Ti rotor at 40 000 rpm for 2 h and 15 min at 4°C. Small and large mitoribosomal subunits were used as a reference of migration and were immunodecorated by antibodies against marker proteins of the mt‐SSU (mS29; HUGO protein name: DAP3) and the mt‐LSU (uL13m; HUGO protein name: MRPL13). (C) Effect of ONC201‐mediated ClpP activation on casein hydrolysis in the PAGE‐based fluorescent assay. Mitochondrial lysates (50 μg) from wild‐type and KO cells were used as a source of ClpP activity, and incubated with ONC201 at a final concentration of 100 μM. Upper panels: Milk casein isoforms (α, β and k) are depicted to the left; molecular weight markers (M; Precision Plus Protein Dual Color Standards, <t>Bio‐Rad)</t> are indicated to the right. Middle panels: Total densitometric profile of fluorescent substrate was determined for each lane. Lower panels: Coomassie blue staining (CBS) of mitochondrial proteins was used as a loading control. (D) Chemical structure of DA29 (ClpP activator) and DA28 (negative control). (E) Effect of ClpP chemoactivation by DA29 on casein hydrolysis in the PAGE‐based fluorescent assay; DA28 was used as a negative control. For description, see panel C.
    Clarity Western Ecl Substrate Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecl+a/Clarity+Western+ECL+Substrate/pmc13675934-67-6-10
    Average 99 stars, based on 1 article reviews
    clarity western ecl substrate bio rad - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    97
    Bio-Rad clarity max western ecl substrate
    Targeted activation of endogenous mitochondrial ClpP. (A) Western blots of mitochondrial lysates (30 μg) from wild‐type and ClpP‐KO HEK293T cells were probed with antibodies against ClpP and α‐tubulin, the latter being used as a loading control. (B) Analysis of ClpP and ClpX assembly was carried out in wild‐type HEK293T mitochondria through isokinetic sucrose gradient centrifugation. Mitochondrial lysate (0.65 mg) was centrifuged on a 10%–30% gradient using the SW 60Ti rotor at 40 000 rpm for 2 h and 15 min at 4°C. Small and large mitoribosomal subunits were used as a reference of migration and were immunodecorated by antibodies against marker proteins of the mt‐SSU (mS29; HUGO protein name: DAP3) and the mt‐LSU (uL13m; HUGO protein name: MRPL13). (C) Effect of ONC201‐mediated ClpP activation on casein hydrolysis in the PAGE‐based fluorescent assay. Mitochondrial lysates (50 μg) from wild‐type and KO cells were used as a source of ClpP activity, and incubated with ONC201 at a final concentration of 100 μM. Upper panels: Milk casein isoforms (α, β and k) are depicted to the left; molecular weight markers (M; Precision Plus Protein Dual Color Standards, <t>Bio‐Rad)</t> are indicated to the right. Middle panels: Total densitometric profile of fluorescent substrate was determined for each lane. Lower panels: Coomassie blue staining (CBS) of mitochondrial proteins was used as a loading control. (D) Chemical structure of DA29 (ClpP activator) and DA28 (negative control). (E) Effect of ClpP chemoactivation by DA29 on casein hydrolysis in the PAGE‐based fluorescent assay; DA28 was used as a negative control. For description, see panel C.
    Clarity Max Western Ecl Substrate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecl+a/Clarity+Max+Western+ECL+Substrate/pmc13651822-284-12-17
    Average 97 stars, based on 1 article reviews
    clarity max western ecl substrate - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    Image Search Results


    Targeted activation of endogenous mitochondrial ClpP. (A) Western blots of mitochondrial lysates (30 μg) from wild‐type and ClpP‐KO HEK293T cells were probed with antibodies against ClpP and α‐tubulin, the latter being used as a loading control. (B) Analysis of ClpP and ClpX assembly was carried out in wild‐type HEK293T mitochondria through isokinetic sucrose gradient centrifugation. Mitochondrial lysate (0.65 mg) was centrifuged on a 10%–30% gradient using the SW 60Ti rotor at 40 000 rpm for 2 h and 15 min at 4°C. Small and large mitoribosomal subunits were used as a reference of migration and were immunodecorated by antibodies against marker proteins of the mt‐SSU (mS29; HUGO protein name: DAP3) and the mt‐LSU (uL13m; HUGO protein name: MRPL13). (C) Effect of ONC201‐mediated ClpP activation on casein hydrolysis in the PAGE‐based fluorescent assay. Mitochondrial lysates (50 μg) from wild‐type and KO cells were used as a source of ClpP activity, and incubated with ONC201 at a final concentration of 100 μM. Upper panels: Milk casein isoforms (α, β and k) are depicted to the left; molecular weight markers (M; Precision Plus Protein Dual Color Standards, Bio‐Rad) are indicated to the right. Middle panels: Total densitometric profile of fluorescent substrate was determined for each lane. Lower panels: Coomassie blue staining (CBS) of mitochondrial proteins was used as a loading control. (D) Chemical structure of DA29 (ClpP activator) and DA28 (negative control). (E) Effect of ClpP chemoactivation by DA29 on casein hydrolysis in the PAGE‐based fluorescent assay; DA28 was used as a negative control. For description, see panel C.

    Journal: The FASEB Journal

    Article Title: Elucidating the Molecular Impact of DA29‐Induced ClpP Chemoactivation on Mitochondrial Function

    doi: 10.1096/fj.202603398RR

    Figure Lengend Snippet: Targeted activation of endogenous mitochondrial ClpP. (A) Western blots of mitochondrial lysates (30 μg) from wild‐type and ClpP‐KO HEK293T cells were probed with antibodies against ClpP and α‐tubulin, the latter being used as a loading control. (B) Analysis of ClpP and ClpX assembly was carried out in wild‐type HEK293T mitochondria through isokinetic sucrose gradient centrifugation. Mitochondrial lysate (0.65 mg) was centrifuged on a 10%–30% gradient using the SW 60Ti rotor at 40 000 rpm for 2 h and 15 min at 4°C. Small and large mitoribosomal subunits were used as a reference of migration and were immunodecorated by antibodies against marker proteins of the mt‐SSU (mS29; HUGO protein name: DAP3) and the mt‐LSU (uL13m; HUGO protein name: MRPL13). (C) Effect of ONC201‐mediated ClpP activation on casein hydrolysis in the PAGE‐based fluorescent assay. Mitochondrial lysates (50 μg) from wild‐type and KO cells were used as a source of ClpP activity, and incubated with ONC201 at a final concentration of 100 μM. Upper panels: Milk casein isoforms (α, β and k) are depicted to the left; molecular weight markers (M; Precision Plus Protein Dual Color Standards, Bio‐Rad) are indicated to the right. Middle panels: Total densitometric profile of fluorescent substrate was determined for each lane. Lower panels: Coomassie blue staining (CBS) of mitochondrial proteins was used as a loading control. (D) Chemical structure of DA29 (ClpP activator) and DA28 (negative control). (E) Effect of ClpP chemoactivation by DA29 on casein hydrolysis in the PAGE‐based fluorescent assay; DA28 was used as a negative control. For description, see panel C.

    Article Snippet: Chemiluminescent signals were detected using the Clarity Western ECL substrate (Bio‐Rad) and the ChemiDoc MP Imaging System (Bio‐Rad).

    Techniques: Activation Assay, Western Blot, Control, Analysis, Gradient Centrifugation, Migration, Marker, Fluorescence, Activity Assay, Incubation, Concentration Assay, Molecular Weight, Staining, Negative Control