ec monolayers (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc ec monolayers
Ec Monolayers, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 10034 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ec+buffer/RIPA+Buffer/pm39302113-77-7-16
Average 99 stars, based on 10034 article reviews

ec monolayers - by Bioz Stars, 2026-11

99/100 stars

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Western Blot:

Article Title: Bacterial stimulation triggers IRF7-mediated IFN-λ production in hepatocytes to attenuate sepsis
Article Snippet: .. The sequences of the primers used in this study are listed below, mIRF7 F: TACCCTGGAAGCATTTCGGT mIRF7 R: CACAGCGGAAGTTGGTCTTC mIfnl2 F: TGGGCCAGCCTCTTCATAC mIfnl2 R: GGGTGAGCAGGCGAAACA mIfnl3 F: CCAGTGGAAGCAAAGGATTGCC mIfnl3 R: GCACCTCATGTCCTTCTCAAGC mIfnlr1 F: ATGCCGTCGCTGGAACTGAAGT mIfnlr1 R: AGCACCTTGCTGGAGAGGAATC AR TIC LE IN PR ES S mTlr4 F: CCTGTGGACAAGGTCAGCAACTC mTlr4 R: CACTCAGACTCGGCACTTAGCAC mMDA5 F: GGATTCAGGCACCATGGGAA mMDA5 R: TGGTTGGGCCACTTCCATTT mRIG-I F: TGTGCCCCTACTGGTTGTG mRIG-I R: TCGTGAGAACACAGTTGCCT mGAPDH F: CAGTGGCAAAGTGGAGATTGTTGɑ mGAPDH R: TCGCTCCTGGAAGATGGTGAT hIRF7 F: AGCGGCTGCTATGAGGGGCT hIRF7 R: GCCACAGCCCAGGCCTTGAA hIFNL2 F: AGTCGCTTCTGCTGAAGGAC hIFNL2 R: TCCAGAACCTTCAGCGTCAG hIFNL3 F: TAAGAGGGCCAAAGATGCCTT hIFNL3 R: CTGGTCCAAGACATCCCCC hGAPDH F: GAGTCAACGGATTTGGTCGT hGAPDH R: GACAAGCTTCCCGTTCTCAG 2.12 Western Blotting Analysis Cells treated with E. coli or left untreated were lysed using RIPA buffer (9806, CST) supplemented with a protease/phosphatase inhibitor cocktail (5872, CST). ..

Analysis:

Saline:

Article Title: SLMO2 promotes chemoresistance in glioblastoma by regulating BNIP3-dependent mitophagy via the NF-κB/HIF-1α axis.
Article Snippet: .. Samples were washed with ice-cold phosphate-buffered saline and lysed in radioimmunoprecipitation assay buffer (RIPA buffer, CST, #9806) on ice for 30 min. .. The samples were prepared for loading by adding a 6 × Protein Loading Buffer (TransGen, DL101-02) and heating for 10 min at 100 ◦C.

Radio Immunoprecipitation:

Article Title: Onion Peel-Derived Exosome-Like Nanovesicles as a Novel Therapeutic Approach for Inflammatory Bowel Disease
Article Snippet: Pierce BCA protein assay kit (Cat. No. 23227) was procured from Thermo Scientific (Waltham, MA, USA). .. Radioimmunoprecipitation assay buffer (RIPA buffer, Cat. No. 9806), total NF-κB (Cat. No. 8242S), phosphorylated NF-κB (Cat. No. 3036S), total ERK (Cat. No. 9102S), phosphorylated ERK (Cat. No. 9201L), and E-cadherin (Cat. No. 14472S) were purchased from Cell Signalling Technology Inc. (Danvers, MA, USA). .. N-cadherin (Cat. No. E-AB70061) was purchased from Elabscience Biotechnology Co. (Wuhan, Hubei, Cina).

BIA-KA:

Article Title: Polymerase iota coordinates adaptation to replication stress in BRCA2-deficient mammary epithelial cells.
Article Snippet: .. Cells were lysed in RIPA buffer (CST, 9803/9806) supplemented with protease and phosphatase inhibitors (CST, 5872) and PMSF (CST, 8553), and protein concentrations were determined by BCA assay (Thermo Scientific, A53225). .. Equal amounts of total protein were resolved by SDS-PAGE (Bio-Rad, 4561084), transferred to PVDF membranes (Bio-Rad, 1620177), and probed with the following antibodies: α-BRCA2 (1:1000, CST Cat# 10741, RRID: AB_2797730), α-POLI (1:2000, Abcam Cat# ab157244, ABclonal Cat# A1942, RRID: AB_2763968), α-RAD52 (1:1000, Abcam Cat# ab124971, RRID: AB_10971685), α-β-Actin (1:2000, CST Cat# 3700, RRID: AB_2242334) and α-Vinculin (1:2000, CST Cat# 13901, RRID: AB_2728768).

Isolation:

Article Title: Microglial SIRT6 confers protection against neuroinflammation-associated depression through NRF2-HO1 signaling
Article Snippet: The following primary antibodies were used: anti-IBA1 (ab178846, Abcam), anti-Sirt6 (ab191385, Abcam), and anti-GFAP (ab7260, Abcam). .. Hippocampal tissues or isolated microglia were lysed in RIPA buffer (Cell Signaling Technology, 9806) supplemented with protease and phosphatase inhibitor cocktails (Roche, 11836170001, 04906837001). .. Protein concentrations were determined using the BCA protein assay kit (Pierce, 23225).

Article Title: Comparative analysis of select 3D human mesenchymal stromal cell-derived spheroid models for adipogenic differentiation
Article Snippet: .. Proteins were isolated using RIPA Buffer (Cell Signalling Technologies, Danvers, MA, USA). ..

Cell-Signaling:

Article Title: Ibrutinib-triggered matriptase maintains extracellular CD19 and limits antigen escape in B-cell malignancy
Article Snippet: .. Cell lysates were prepared in RIPA buffer (Cell Signaling Technology [CST], Danvers, MA, USA) supplemented with protease (Roche, Basel, Switzerland) and phosphatase inhibitors (AdooQ BioScience, Irvine, CA, USA). .. Protein concentrations were quantified using a SpectraMax iD3 reader (Molecular Devices, San Jose, CA, USA).

Recombinant:

Article Title: Protocol for endothelial cell-derived extracellular vesicle isolation and characterization.
Article Snippet: Mouse Anti-CD9 (Tetraspanin) Dilution 1 in 1000 Millipore Cat#CBL162 Rabbit Anti-GM130 (Golgi marker) Dilution 1 in 1000 Abcam Clone:EP892Y Cat#ab52649 Rabbit Anti-Syntenin-1 (Luminal marker) Dilution 1 in 1000 Abcam Clone: EPR8102 Cat#ab133267 Mouse Anti-TSG101 (Luminal marker) Dilution 1 in 2000 Abcam Clone:4A10 Cat#ab83 Rabbit Anti-VCAM-1 (Endothelial cell activation marker) Dilution 1 in 1000 Abcam Clone: EPR5047 Cat#ab134047 Anti-Mouse IgG (H+L), HRP conjugate Dilution 1 in 20000 Promega W402b Anti-Rabbit IgG (H+L), HRP conjugate Dilution 1 in 50000 Promega W401b Chemicals, peptides, and recombinant proteins NanoStandards 0,100 μm Cert mean diameter: 0,096 μm Exp. .. Uncertainty: 0,010 μm Size CV: 5,8 % Solids: 1,0 % Applied MicroSpheres Cat#75009-03 Lot:WS0024.2212 Recombinant Human tumour necrosis factor-α Biotechne Cat#210-TA-020/CF Amersham ECL Rainbow Marker - Full range VWR International Ltd Cat#RPN800E EGM-2 media supplemented with BulletKit Lonza Cat#CC-3162 NuPAGE MOPS SDS Running Buffer (20X), 500 mL ThermoFisher Scientific UK Ltd Cat#NP0001 Supersignal West Dura Extended Duration Substrate ThermoFisher Scientific UK Ltd Cat#34076 RIPA Buffer (10X) Cell Signalling Cat9806 (Continued on next page) 4 STAR Protocols 7, 104887, December 18, 2026 CRITICAL: Uranyl acetate is hazardous and should be handled and disposed of in accordance with institutional safety procedures and applicable local regulations. ..

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